ANTIOXDATIVE ACTIVITIES OF WILD MACRO FUNGI GANODERMA APPLANATUM (PERS.) PAT.

The present research work was under taken to evaluate antioxidant activity and quantitative determination of phytoconstituents of various solvent extracts of wild macro fungi Ganoderma applanatum (Pers.) Pat. using in vitro models. The total phenolic content was estimated using Folin-Ciocalteu method and total flavonoid content was determined using aluminum chloride. In vitro antioxidant activity of solvent extracts were studied in different systems including DPPH scavenging assay, reducing power assay, metal chelating activity, superoxide radical assay and hydrogen peroxide activity along with standard. The results revealed that, the total phenolics and flavonoids were high and extracts showed good antioxidant capacity in DPPH radical scavenging assay compared to other in vitro models. The methanol extract can be considered as a valuable source of antioxidant products as the obtained values are more or less near to the used standard compounds. The present study concludes that methanolic extract of study species is effective in scavenging free radicals and has the potential to be powerful antioxidant. This potent antioxidant activity may be attributed to its high phenolic and flavonoid content. Keywords: Antioxidant activity, Ganoderma applanatum, Radical scavenging, Phenolics, flavonoids

Analgesic use and potential hearing loss: a pharmacovigilance study

Background: Hearing loss is the most common sensory deficit in humans today. In India, as per WHO there are approximately 63 million people, who are suffering from significant auditory impairment and estimated prevalence at 6.3% in Indian population. The objective of the study was to determine the potential hearing loss in patients on analgesics.Methods: Prospective, observational study examined the association between analgesic use and potential hearing loss in 30 patients receiving NSAIDS. Data was collected using specialized designed case record form. The clinical signs like onset of tinnitus, appearance of another sound that didn't exist before, sinus infection or a respiratory illness, development of vertigo or spinning sensation were looked for. Audiometry test was advised for all the patients receiving analgesics.Results: Total of 30 patients were included in the study. Male predominance was noted Maximum patients were in the age group between 35-45 years (33.33%). The socioeconomic status was analyzed using revised kuppuswamy scale. 30% (n=09) of patients were in lower class. Majority of the patients were reported with road traffic accidents (n=19; 63.33%) and received analgesics for more than 2 weeks. Patients received different combination of NSAIDs among which aceclofenac was most commonly prescribed. 11 (36.66%) patients had hearing loss received either diclofenac or aceclofenac for more than 25 days.Conclusions: Regular usage of NSAIDS may increase the risk of hearing loss and the impact could be larger on younger individuals. Hence, extra precaution to be taken while prescribing NSAIDS

System-wide Perturbation Analysis with Nearly Complete Coverage of the Yeast Proteome by Single-shot Ultra HPLC Runs on a Bench Top Orbitrap

Yeast remains an important model for systems biology and for evaluating proteomics strategies. In-depth shotgun proteomics studies have reached nearly comprehensive coverage, and rapid, targeted approaches have been developed for this organism. Recently, we demonstrated that single LC-MS/MS analysis using long columns and gradients coupled to a linear ion trap Orbitrap instrument had an unexpectedly large dynamic range of protein identification (Thakur, S. S., Geiger, T., Chatterjee, B., Bandilla, P., Frohlich, F., Cox, J., and Mann, M. (2011) Deep and highly sensitive proteome coverage by LC-MS/MS without prefractionation. Mol. Cell Proteomics 10, 10.1074/mcp.M110.003699). Here we couple an ultra high pressure liquid chromatography system to a novel bench top Orbitrap mass spectrometer (Q Exactive) with the goal of nearly complete, rapid, and robust analysis of the yeast proteome. Single runs of filter-aided sample preparation (FASP)-prepared and LysC-digested yeast cell lysates identified an average of 3923 proteins. Combined analysis of six single runs improved these values to more than 4000 identified proteins/run, close to the total number of proteins expressed under standard conditions, with median sequence coverage of 23%. Because of the absence of fractionation steps, only minuscule amounts of sample are required. Thus the yeast model proteome can now largely be covered within a few hours of measurement time and at high sensitivity. Median coverage of proteins in Kyoto Encyclopedia of Genes and Genomes pathways with at least 10 members was 88%, and pathways not covered were not expected to be active under the conditions used. To study perturbations of the yeast proteome, we developed an external, heavy lysine-labeled SILAC yeast standard representing different proteome states. This spike-in standard was employed to measure the heat shock response of the yeast proteome. Bioinformatic analysis of the heat shock response revealed that translation-related functions were down-regulated prominently, including nucleolar processes. Conversely, stress-related pathways were up-regulated. The proteomic technology described here is straightforward, rapid, and robust, potentially enabling widespread use in the yeast and other biological research communities

Image based Seen Detection in Real Time Video Interpretation for Surveillance Systems using Support Vectors Machine

People that are perpetually hunting for knowledge will benefit from data acquisition. The early phase in video data acquisition is splitting the video into images. Many images are tiny and don't reveal a lot about the picture's information. Scene boundary identification, or video segmentation into action sequences, enables a more complete comprehension of the image sequence by classifying images based on comparable visual content. The purpose of this article is to discuss video scene recognition, particularly video structure extraction for pattern comparison with significant properties. The article designed and developed a methodology that would include stages for image collection, detecting commonalities among frames, selecting important frames, and detecting the time at where the relevant frame is identified. The pictures are generated by Python's OpenCV and scene classification metrics are used to assess the method. As assessed by numerous parameters, the findings shows that scene identification and accuracy are considerable. Furthermore, we investigated and researched contemporary identification and assessment techniques. Moreover, we have tested extensively our research framework on a variety of publicly available event video databases, and these outperformed several futuristic techniques. The outcomes of this research can be utilized to generate real-time definitional video assessments

Anatomical variations of sinonasal region: a CT scan study

Multislice CT is currently the imaging modality of choice for evaluating PNS & adjacent structures. Such a method has been increasingly utilized in the assessment of anatomical variations, allowing their accurate identification with high anatomical details. Some anatomical variations may predispose to sinonasal disease, constituting areas of high risk for injuries & complications during surgical procedures. Therefore, the recognition of such variations is critical in the preoperative evaluation for endoscopic surgery

Specific absorbed fraction of energy of silicon-boron alloys

213-217We have studied the energy absorption buildup factors and specific absorbed fraction of energy for the silicon-boron alloys of different composition such as alloy A-Si0.95-B0.05, alloy B- Si0.9-B0.1, alloy C- Si0.8-B0.2, alloy D- Si0.7-B0.3, alloy E- Si0.6-B0.4 and alloy F- Si0.5-B0.5. for wide energy range (0.015–15 MeV) up to the penetration depth of 40 mfp using geometric progression fitting method. Buildup factors increase with the increase in the penetration depth. It has been found that the shielding parameters such as mass attenuation coefficient, effective atomic number and buildup factor values are larger for the silicon-boron alloy Si0.95-B0.05 than the other studied silicon-boron alloys. Specific absorbed fraction of energy is maximum for the silicon-boron alloy Si0.95-B0.05. Hence, we can conclude that the silicon-boron alloy Si0.95-B0.05 is a good absorber of X-rays, gamma and neutrons among the studied alloys. The present study is useful in the field of radiation shielding

Beach Morphological Characteristics and Coastal Processes Along Dakshina Kannada Coast, West Coast of India

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv

Baseline Scenario of Rainy Season Pearl Millet in Haryana - Working Paper Series No. 40

Pearl millet has been cultivated in Haryana as a dual purpose crop where grain and fodder are most often valued equally, which supports poor smallholders and livestock in the harsh agro-climatic region. Currently, the productivity levels are low because of limited adoption of dryland technologies by the poor. The HOPE project aimed at increasing the productivity of pearl millet by 35-40 % over the base level by introducing on-shelf technology and improved management practices in the targeted clusters over a period of four years. In this regard, the baseline survey was carried out in the primary project intervention area (HOPE) where improved technologies have been introduced, and in matching control villages with comparable agro-ecological and market conditions in non-intervention areas (non-HOPE), where improved technologies have not been made. The objective of this baseline survey was to appraise the existing situation of the targeted cluster villages with respect to adoption of technologies, productivity, income, yield gaps and other socioeconomic issues. Pearl millet is the major crop in the rainy season followed by mustard and gram in the rabi (postrainy) season. The average productivity of pearl millet ranges from 1.54 – 1.79 t/ha as against the potential yield of 2.5 – 2.9 t/ha, leaving a yield gap of 58-101%. In the HOPE clusters, adoption rate of hybrids is at the peak, covering around 48% (out of 124.5 ha) under public bred hybrids (HHB 67, HHB 67 Improved), and 50 % under proprietary hybrids. While the same in non-HOPE area was 35% (out of 54.8 ha) under public bred hybrids, and rest of the area is under proprietary hybrids. The yield gap of improved hybrids of pearl millet was estimated as 58-101%, as compared to the potential yield, which shows further scope for improvement in productivity levels. On an average, farmers were reaping a net benefit of is Rs.2108 per ha in HOPE areas, while it was Rs.6238 per ha in non-HOPE area, after accounting the total costs. Farmers most preferred traits in public hybrids of pearl millet inter alia include more palatable grain and fodder quality and disease-pest-moisture stress. Key critical constraints expressed by the farmers are shortage of labour especially during harve

Specific absorbed fraction of energy of silicon-boron alloys

We have studied the energy absorption buildup factors and specific absorbed fraction of energy for the silicon-boron alloys of different composition such as alloy A-Si0.95-B0.05, alloy B- Si0.9-B0.1, alloy C- Si0.8-B0.2, alloy D- Si0.7-B0.3, alloy E- Si0.6-B0.4 and alloy F- Si0.5-B0.5. for wide energy range (0.015–15 MeV) up to the penetration depth of 40 mfp using geometric progression fitting method. Buildup factors increase with the increase in the penetration depth. It has been found that the shielding parameters such as mass attenuation coefficient, effective atomic number and buildup factor values are larger for the silicon-boron alloy Si0.95-B0.05 than the other studied silicon-boron alloys. Specific absorbed fraction of energy is maximum for the silicon-boron alloy Si0.95-B0.05. Hence, we can conclude that the silicon-boron alloy Si0.95-B0.05 is a good absorber of X-rays, gamma and neutrons among the studied alloys. The present study is useful in the field of radiation shielding