Thymosin β4 as basis for creation of a reparation preparation of new generation

On the basis of its multipurpose activities during regeneration of fabrics in various experiments on animals, Thymosin β4 has the potential for new researches, in kidneys and a liver, and also recovery of a spinal cord, bones and injury of ligaments. Besides, it can be useful in case of treatment of a wide range of other diseases, including concerning consequences of old bacterial damages and viral infection

Analysis of rare type of plants from botanical graden collection at SRU “Belsu” (Belgorod, Russia)

The aim of the study was the comprehensive analysis of rare plants from the collection fund of the botanical garden at the National Research University “BelSU

Role of membrane lipids in the regulation of erythrocytic oxygen-transport function in cardiovascular diseases

The composition and condition of membrane lipids, the morphology of erythrocytes, and hemoglobin distribution were explored with the help of laser interference microscopy (LIM) and Raman spectroscopy. It is shown that patients with cardiovascular diseases (CVD) have significant changes in the composition of their phospholipids and the fatty acids of membrane lipids. Furthermore, the microviscosity of the membranes and morphology of the erythrocytes are altered causing disordered oxygen transport by hemoglobin. Basic therapy carried out with the use of antiaggregants, statins, antianginals, beta-blockers, and calcium antagonists does not help to recover themorphofunctional properties of erythrocytes. Based on the results the authors assume that, for the relief of the ischemic crisis and further therapeutic treatment, it is necessary to include, in addition to cardiovascular disease medicines, medication that increases the ability of erythrocytes’ hemoglobin to transport oxygen to the tissues. We assume that the use of LIM and Raman spectroscopy is advisable for early diagnosis of changes in the structure and functional state of erythrocytes when cardiovascular diseases develop

Ion channel TRPA₁ is a promising therapeutic target for treatment of pain

In the last time information about the role of TRPA₁ in pain and cold sensitivity, as well as in the formation and maintenance of inflammation is increasing in scientific literature. Given this information, the interest for search and study of pharmacological agents, which selectively blocked of TRPA₁ and reduced the severity of pain and inflammation is increasin

Сlinical and epidemiological features of rotavirus infections in infants

Objective: to study the clinical and epidemiological characteristics, the nature of the course and outcomes of the disease in children with rotavirus mono- and mixed viral - bacterial lesions of the gastrointestinal tract.Materials and methods: in this work the results of clinical observations of 143 patients with rotavirus infection before the age of 1 year. At the same time 69 children were diagnosed with rotavirus gastroenteritis, while 74 people. there has been a combination of rotavirus with the pathogenic microflora.Results and conclusion: The clinical picture of combined forms of rotavirus infection differed for signs of defeat all parts of the gastrointestinal tract, inflammatory changes in the blood and coprogram. Combined damage to the intestinal mucosa and the associated inflammation conditioned a «non-smooth» during the acute period of the disease, which significantly increased the duration of treatment and hospital stay

Minimisation of the viral contamination risk of heterologous immunoglobulins in the context of the requirements of the State Pharmacopoeia of the Russian Federation

To ensure the safety and to secure the approval of injectable medicinal products based on antigen-specific immunoglobulins of animal origin, it is necessary to exclude their contamination with adventitious human pathogens. Ensuring the viral safety of heterologous immunoglobulins presents a major challenge, because the State Pharmacopoeia of the Russian Federation, 14 edition, lacks production stage-specific viral safety requirements for such medicinal products. The aim of the study was to analyse the requirements set forth in general and individual monographs of the State Pharmacopoeia of the Russian Federation, the European Pharmacopoeia, (10th edition), the British Pharmacopoeia (2019), the United States Pharmacopoeia (USP 43–NF 38), the Japanese Pharmacopoeia (17th edition), as well as the recommendations of the European Medicines Agency and the World Health Organisation concerning the viral safety of medicinal products for human use based on heterologous antigen-specific immunoglobulins. The authors analysed regulatory requirements for the following: serum/plasma-producing animals; immunisation antigens for the animals; quarantine of the animals; viral contamination tests for immune animal serum/plasma pools; model viruses to validate viral inactivation/removal processes at different stages of vaccine production; viral load reduction at each inactivation/ removal step; testing of materials obtained at critical production stages. The authors drafted sections for quality standards on production stage-specific measures to minimise the viral contamination risk of medicinal products for human use based on heterologous immunoglobulins, which they proposed for inclusion to the State Pharmacopoeia of the Russian Federation

НАРУШЕНИЯ МЕТАБОЛИЧЕСКОЙ АКТИВНОСТИ КИШЕЧНОЙ МИКРОФЛОРЫ И МЕСТНОГО ИММУНИТЕТА ПРИ РОТАВИРУСНОЙ ИНФЕКЦИИ

The paper describes the research on metabolic activity of intestinal microflora and secretory immunoglobulin A (sIgA) content in coprofiltrate of rotavirus infection patients depending on disease course. It is established that a long-lasting clinical oppression of metabolic processes of microbiocenosis and local immunity deficiency define a rough course of rotavirus infection. Исследована метаболическая активность микрофлоры кишечника и содержание секреторного иммуноглобулина А (sIgA) в копрофильтрате у больных ротавирусной инфекцией (РИ) в зависимости от характера течения заболевания. Установлено, что длительно сохраняющееся в течение болезни угнетение метаболических процессов микробиоценоза и недостаточность местного иммунитета определяют негладкий характер течения РИ.

Cholera in Azov area

The purpose of research is analysis of clinical course and treatment results of patients with cholera in the Azov area. Materials and methods. During the period from 29.05.2011 to 19.08.2011 33 cases of cholera (32 adults and 1 child) and 25 vibrio carriers (22 adults and 3 children), which were caused by toxigenic strains of Vibrio cholera El Tor serogroup O1 Ogawa. Results. Likely factors of disease transmission in Mariupol are sea and river water, and the fish that were caught in the waters of the city. Typical and watery diarrhoea, vomiting, abdominal pain and lack of normal body temperature, dehydration syndrome, characterized clinical cholera for adults in most cases. The mean duration of diarrhoea was 6,6 days. At 46.9% observed atypical symptoms in 10 (31,3%) – abdominal pain (1 patient cramping in 7 cases, localized in the epigastria region, at 2-over stomach). In 5 patients (15,6%) had an increase in body temperature to 37,2–37,7 degrees Celsius. In 15 (46,9%) patients had severe nausea accompanied by vomiting. Easy for cholera was observed in 1 (3.1%), moderate – in 14 (43,8%), heavy – in 17 (53,1%) patients. Dehydration I level is set at 4 (12,5%), II – from 6 (18,7%), III – in 18 (56,3%), IV – 4 (12,5%) patients. Cholera outbreak was characterized by a predominance of severe disease and severe dehydration (III and IV), which was observed in 68.8% of patients. The decisive factor in the treatment of cholera patients was initiated in a timely manner rehydration therapy, in particular the introduction of the solution «Trisol». Against the background of rehydration therapy hyperkalaemia was observed in 9,4% of cases, vascular rehydration at 9,4%, the cell rehydration in 3,1% of patients. Fatal accidents cholera outbreaks have not been observed. Conclusion. Clinical diagnosis of cholera and the provision of medical care in the prehospital phase were poor, indicating the need for systematic conducting training seminars among experts of ambulance, as well as doctors of primary medical network. Cholera hospitals (or base hospitals) must be equipped with a mobile diagnostics (ultrasound, X-rays) and modern laboratory equipment. The required list of drug supply cholera hospitals should be expanded in view of possible complications and comorbidities

Синтез и характеристика фотоактивных арилазидных производных пептидов семакса и Pro-Gly-Pro

The photoaffine arylazide derivatives of peptides semax and Pro-Gly-Pro have been synthesized. It was shown that these compounds have cytoptotective action in culture of PC12 cells under conditions of oxidative stress.Осуществлен синтез п-азидотетрафторбензойных производных пептидов семакса и Pro-Gly-Pro. Показано, что полученные фотоаффинные арилазидные зонды обладают цтопротективным действием на культивируемые клетки PC12 в условиях окислительного процесс

К ПРОБЛЕМЕ ДИАГНОСТИКИ ВРОЖДЕННОГО ТУБЕРКУЛЕЗА (КЛИНИЧЕСКИЙ СЛУЧАЙ)

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