1,722 research outputs found

    Drosophila embryos as model systems for monitoring bacterial infection in real time.

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    Journal ArticleResearch Support, Non-U.S. Gov'tDrosophila embryos are well studied developmental microcosms that have been used extensively as models for early development and more recently wound repair. Here we extend this work by looking at embryos as model systems for following bacterial infection in real time. We examine the behaviour of injected pathogenic (Photorhabdus asymbiotica) and non-pathogenic (Escherichia coli) bacteria and their interaction with embryonic hemocytes using time-lapse confocal microscopy. We find that embryonic hemocytes both recognise and phagocytose injected wild type, non-pathogenic E. coli in a Dscam independent manner, proving that embryonic hemocytes are phagocytically competent. In contrast, injection of bacterial cells of the insect pathogen Photorhabdus leads to a rapid 'freezing' phenotype of the hemocytes associated with significant rearrangement of the actin cytoskeleton. This freezing phenotype can be phenocopied by either injection of the purified insecticidal toxin Makes Caterpillars Floppy 1 (Mcf1) or by recombinant E. coli expressing the mcf1 gene. Mcf1 mediated hemocyte freezing is shibire dependent, suggesting that endocytosis is required for Mcf1 toxicity and can be modulated by dominant negative or constitutively active Rac expression, suggesting early and unexpected effects of Mcf1 on the actin cytoskeleton. Together these data show how Drosophila embryos can be used to track bacterial infection in real time and how mutant analysis can be used to genetically dissect the effects of specific bacterial virulence factors.Wellcome TrustBBSR

    Quantum Clock Synchronization Based on Shared Prior Entanglement

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    We demonstrate that two spatially separated parties (Alice and Bob) can utilize shared prior quantum entanglement, and classical communications, to establish a synchronized pair of atomic clocks. In contrast to classical synchronization schemes, the accuracy of our protocol is independent of Alice or Bob's knowledge of their relative locations or of the properties of the intervening medium.Comment: 4 page

    Protection of Pepper Plants from Drought by Microbacterium sp 3J1 by Modulation of the Plant's Glutamine and alpha-ketoglutarate Content: A Comparative Metabolomics Approach

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    Vilchez JI, Niehaus K, Dowling DN, Gonzalez-Lopez J, Manzanera M. Protection of Pepper Plants from Drought by Microbacterium sp 3J1 by Modulation of the Plant's Glutamine and alpha-ketoglutarate Content: A Comparative Metabolomics Approach. FRONTIERS IN MICROBIOLOGY. 2018;9: 17.Drought tolerance of plants such as tomato or pepper can be improved by their inoculation with rhizobacteria such as Microbacterium sp. 3J1. This interaction depends on the production of trehalose by the microorganisms that in turn modulate the phyto-hormone profile of the plant. In this work we describe the characterization of metabolic changes during the interaction of pepper plants with Microbacterium sp. 3J1 and of the microorganism alone over a period of drought. Our main findings include the observation that the plant responds to the presence of the microorganism by changing the C and N metabolism based on its glutamine and alpha-ketoglutarate content, these changes contribute to major changes in the concentration of molecules involved in the balance of the osmotic pressure. These include sugars and amino-acids; the concentration of antioxidant molecules, of metabolites involved in the production of phytohormones like ethylene, and of substrates used for lignin production such as ferulic and sinapic acids. Most of the altered metabolites of the plant when inoculated with Microbacterium sp. 3J1 in response to drought coincided with the profile of altered metabolites in the microorganism alone when subjected to drought, pointing to a response by which the plant relies on the microbe for the production of such metabolites. To our knowledge this is the first comparative study of the microbe colonized-plant and microbe alone metabolomes under drought stress

    Simulations of atomic trajectories near a dielectric surface

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    We present a semiclassical model of an atom moving in the evanescent field of a microtoroidal resonator. Atoms falling through whispering-gallery modes can achieve strong, coherent coupling with the cavity at distances of approximately 100 nanometers from the surface; in this regime, surface-induced Casmir-Polder level shifts become significant for atomic motion and detection. Atomic transit events detected in recent experiments are analyzed with our simulation, which is extended to consider atom trapping in the evanescent field of a microtoroid.Comment: 29 pages, 10 figure

    Ion trap simulations of quantum fields in an expanding universe

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    We propose an experiment in which the phonon excitation of ion(s) in a trap, with a trap frequency exponentially modulated at rate kappa, exhibits a thermal spectrum with an Unruh temperature given by k(B)T=h kappa. We discuss the similarities of this experiment to the response of detectors in a de Sitter universe and the usual Unruh effect for uniformly accelerated detectors. We demonstrate a new Unruh effect for detectors that respond to antinormally ordered moments using the ion's first blue sideband transition

    Nano-displacement measurements using spatially multimode squeezed light

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    We demonstrate the possibility of surpassing the quantum noise limit for simultaneous multi-axis spatial displacement measurements that have zero mean values. The requisite resources for these measurements are squeezed light beams with exotic transverse mode profiles. We show that, in principle, lossless combination of these modes can be achieved using the non-degenerate Gouy phase shift of optical resonators. When the combined squeezed beams are measured with quadrant detectors, we experimentally demonstrate a simultaneous reduction in the transverse x- and y- displacement fluctuations of 2.2 dB and 3.1 dB below the quantum noise limit.Comment: 21 pages, 9 figures, submitted to "Special Issue on Fluctuations & Noise in Photonics & Quantum Optics" of J. Opt.

    Quantum computation with linear optics

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    We present a constructive method to translate small quantum circuits into their optical analogues, using linear components of present-day quantum optics technology only. These optical circuits perform precisely the computation that the quantum circuits are designed for, and can thus be used to test the performance of quantum algorithms. The method relies on the representation of several quantum bits by a single photon, and on the implementation of universal quantum gates using simple optical components (beam splitters, phase shifters, etc.). The optical implementation of Brassard et al.'s teleportation circuit, a non-trivial 3-bit quantum computation, is presented as an illustration.Comment: LaTeX with llncs.cls, 11 pages with 5 postscript figures, Proc. of 1st NASA Workshop on Quantum Computation and Quantum Communication (QCQC 98

    High precision fundamental constants at the TeV scale

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    This report summarizes the proceedings of the 2014 Mainz Institute for Theoretical Physics (MITP) scientific program on "High precision fundamental constants at the TeV scale". The two outstanding parameters in the Standard Model dealt with during the MITP scientific program are the strong coupling constant αs\alpha_s and the top-quark mass mtm_t. Lacking knowledge on the value of those fundamental constants is often the limiting factor in the accuracy of theoretical predictions. The current status on αs\alpha_s and mtm_t has been reviewed and directions for future research have been identified.Comment: 57 pages, 24 figures, pdflate
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