372 research outputs found
IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.
Centrosome amplification is a hallmark of cancer, and centrosome clustering is essential for cancer cell survival. The mitotic kinesin HSET is an essential contributor to this process. Recent studies have highlighted novel functions for intraflagellar transport (IFT) proteins in regulating motors and mitotic processes. Here, using siRNA knock-down of various IFT proteins or AID-inducible degradation of endogenous IFT88 in combination with small-molecule inhibition of HSET, we show that IFT proteins together with HSET are required for efficient centrosome clustering. We identify a direct interaction between the kinesin HSET and IFT proteins, and we define how IFT proteins contribute to clustering dynamics during mitosis using high-resolution live imaging of centrosomes. Finally, we demonstrate the requirement of IFT88 for efficient centrosome clustering in a variety of cancer cell lines naturally harboring supernumerary centrosomes and its importance for cancer cell proliferation. Overall, our data unravel a novel role for the IFT machinery in centrosome clustering during mitosis in cells harboring supernumerary centrosomes
Theory of the c-Axis Penetration Depth in the Cuprates
Recent measurements of the London penetration depth tensor in the cuprates
find a weak temperature dependence along the c-direction which is seemingly
inconsistent with evidence for d-wave pairing deduced from in-plane
measurements. We demonstrate in this paper that these disparate results are not
in contradiction, but can be explained within a theory based on incoherent
quasiparticle hopping between the CuO2 layers. By relating the calculated
temperature dependence of the penetration depth \lambda_c(T) to the c-axis
resistivity, we show how the measured ratio \lambda_c^2(0) / \lambda_c^2(T) can
provide insight into the behavior of c-axis transport below Tc and the related
issue of ``confinement.''Comment: 4 pages, REVTEX with psfig, 3 PostScript figures included in
compressed for
Frequency Characteristics of Visually Induced Motion Sickness
This article was published in the journal, Human Factors [Sage Publications / © Human Factors and Ergonomics Society.]. The definitive version is available at: http://dx.doi.org/10.1177/0018720812469046Objective: The aim of this study was to explore
the frequency response of visually induced motion
sickness (VIMS) for oscillating linear motion in the foreand-
aft axis.
Background: Simulators, virtual environments,
and commercially available video games that create an
illusion of self-motion are often reported to induce
the symptoms seen in response to true motion. Often
this human response can be the limiting factor in the
acceptability and usability of such systems. Whereas
motion sickness in physically moving environments
is known to peak at an oscillation frequency around
0.2 Hz, it has recently been suggested that VIMS peaks
at around 0.06 Hz following the proposal that the
summed response of the visual and vestibular selfmotion
systems is maximized at this frequency. Methods: We exposed 24 participants to random
dot optical flow patterns simulating oscillating foreand-
aft motion within the frequency range of 0.025 to
1.6 Hz. Before and after each 20-min exposure, VIMS was
assessed with the Simulator Sickness Questionnaire.
Also, a standard motion sickness scale was used to rate
symptoms at 1-min intervals during each trial.
Results: VIMS peaked between 0.2 and 0.4 Hz with
a reducing effect at lower and higher frequencies.
Conclusion: The numerical prediction of the
“crossover frequency” hypothesis, and the design
guidance curve previously proposed, cannot be accepted
when the symptoms are purely visually induced.
Application: In conditions in which stationary
observers are exposed to optical flow that simulates
oscillating fore-and-aft motion, frequencies around 0.2
to 0.4 Hz should be avoided
Wild Animals in Our Backyard. A Contextual Approach to the Intrinsic Value of Animals
As a reflection on recent debates on the value of wild animals we examine the question of the intrinsic value of wild animals in both natural and man-made surroundings. We examine the concepts being wild and domesticated. In our approach we consider animals as dependent on their environment, whether it is a human or a natural environment. Stressing this dependence we argue that a distinction can be made between three different interpretations of a wild animal’s intrinsic value: a species-specific, a naturalistic, and an individualistic interpretation. According to the species-specific approach, the animal is primarily considered as a member of its species; according to the naturalistic interpretation, the animal is seen as dependent on the natural environment; and according to the individualistic approach, the animal is seen in terms of its relationship to humans. In our opinion, the species-specific interpretation, which is the current dominant view, should be supplemented—but not replaced by—naturalistic and individualistic interpretations, which focus attention on the relationship of the animal to the natural and human environments, respectively. Which of these three interpretations is the most suitable in a given case depends on the circumstances and the opportunity for the animal to grow and develop according to its nature and capabilities
Quantifying Rates of Evolutionary Adaptation in Response to Ocean Acidification
The global acidification of the earth's oceans is predicted to impact biodiversity via physiological effects impacting growth, survival, reproduction, and immunology, leading to changes in species abundances and global distributions. However, the degree to which these changes will play out critically depends on the evolutionary rate at which populations will respond to natural selection imposed by ocean acidification, which remains largely unquantified. Here we measure the potential for an evolutionary response to ocean acidification in larval development rate in two coastal invertebrates using a full-factorial breeding design. We show that the sea urchin species Strongylocentrotus franciscanus has vastly greater levels of phenotypic and genetic variation for larval size in future CO2 conditions compared to the mussel species Mytilus trossulus. Using these measures we demonstrate that S. franciscanus may have faster evolutionary responses within 50 years of the onset of predicted year-2100 CO2 conditions despite having lower population turnover rates. Our comparisons suggest that information on genetic variation, phenotypic variation, and key demographic parameters, may lend valuable insight into relative evolutionary potentials across a large number of species
Amiloride-sensitive channels in type I fungiform taste cells in mouse
<p>Abstract</p> <p>Background</p> <p>Taste buds are the sensory organs of taste perception. Three types of taste cells have been described. Type I cells have voltage-gated outward currents, but lack voltage-gated inward currents. These cells have been presumed to play only a support role in the taste bud. Type II cells have voltage-gated Na<sup>+ </sup>and K<sup>+ </sup>current, and the receptors and transduction machinery for bitter, sweet, and umami taste stimuli. Type III cells have voltage-gated Na<sup>+</sup>, K<sup>+</sup>, and Ca<sup>2+ </sup>currents, and make prominent synapses with afferent nerve fibers. Na<sup>+ </sup>salt transduction in part involves amiloride-sensitive epithelial sodium channels (ENaCs). In rodents, these channels are located in taste cells of fungiform papillae on the anterior part of the tongue innervated by the chorda tympani nerve. However, the taste cell type that expresses ENaCs is not known. This study used whole cell recordings of single fungiform taste cells of transgenic mice expressing GFP in Type II taste cells to identify the taste cells responding to amiloride. We also used immunocytochemistry to further define and compare cell types in fungiform and circumvallate taste buds of these mice.</p> <p>Results</p> <p>Taste cell types were identified by their response to depolarizing voltage steps and their presence or absence of GFP fluorescence. TRPM5-GFP taste cells expressed large voltage-gated Na<sup>+ </sup>and K<sup>+ </sup>currents, but lacked voltage-gated Ca<sup>2+ </sup>currents, as expected from previous studies. Approximately half of the unlabeled cells had similar membrane properties, suggesting they comprise a separate population of Type II cells. The other half expressed voltage-gated outward currents only, typical of Type I cells. A single taste cell had voltage-gated Ca<sup>2+ </sup>current characteristic of Type III cells. Responses to amiloride occurred only in cells that lacked voltage-gated inward currents. Immunocytochemistry showed that fungiform taste buds have significantly fewer Type II cells expressing PLC signalling components, and significantly fewer Type III cells than circumvallate taste buds.</p> <p>Conclusion</p> <p>The principal finding is that amiloride-sensitive Na<sup>+ </sup>channels appear to be expressed in cells that lack voltage-gated inward currents, likely the Type I taste cells. These cells were previously assumed to provide only a support function in the taste bud.</p
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