1,216 research outputs found
Different forms of the bovine PrP gene have five or six copies of a short, G-C-rich element within the protein-coding exon
Current models of the virus-like agents of scrapie and bovine spongiform encephalopathy (BSE) have to take into account that structural changes in a host-encoded protein (PrP protein) exhibit an effect on the time course of these diseases and the survival time of any man or animal exposed to these pathogens. We report here the sequence of different forms of the bovine PrP gene which contain either five or six copies of a short, G-C-rich element which encodes the octapeptide Pro-His-Gly-Gly-Gly-Trp-Gly-Gln or its longer variants Pro-Gln/His-Gly-Gly-Gly-Gly-Trp-Gly-Gln. Out of 12 cattle, we found eight animals homozygous for genes with six copies of the Gly-rich peptide (6:6), while four were heterozygous (6:5). Two confirmed cases of BSE occurred in (6: 6) homozygous animals. Bovine spongiform encephalopathy (BSE) is a transmissible disease (Fraser et al., 1988; Dawson et al., 1990; Barlow & Middleton, 1990) which produces neuropathological lesions in cattle similar to those seen in ovine scrapie (Wells et al., 1987) and the rare human dementias Creutzfeldt-Jakob disease (CJD) and Gerstmann-Str/iussler syndrome (GSS) (Beck & Daniel, 1987). A cellular membrane protein (PrP) has a key role in the transmission and development of these diseases. This protein accumulates in the brain and other tissues during the protracted time course of these diseases and, in a disease-specific, protease-resistant isoform (SAF-PrP), has been purified by subcellular fractionation of scrapie
Lateral current density fronts in asymmetric double-barrier resonant-tunneling structures
We present a theoretical analysis and numerical simulations of lateral
current density fronts in bistable resonant-tunneling diodes with Z-shaped
current-voltage characteristics. The bistability is due to the charge
accumulation in the quantum well of the double-barrier structure. We focus on
asymmetric structures in the regime of sequential incoherent tunneling and
study the dependence of the bistability range, the front velocity and the front
width on the structure parameters. We propose a sectional design of a structure
that is suitable for experimental observation of front propagation and discuss
potential problems of such measurements in view of our theoretical findings. We
point out the possibility to use sectional resonant-tunneling structures as
controllable three-terminal switches.Comment: to appear in J.Appl.Phy
Electron mean free path from angle-dependent photoelectron spectroscopy of aerosol particles
We propose angle-resolved photoelectron spectroscopy of aerosol particles as
an alternative way to determine the electron mean free path of low energy
electrons in solid and liquid materials. The mean free path is obtained from
fits of simulated photoemission images to experimental ones over a broad range
of different aerosol particle sizes. The principal advantage of the aerosol
approach is twofold. Firstly, aerosol photoemission studies can be performed
for many different materials, including liquids. Secondly, the size-dependent
anisotropy of the photoelectrons can be exploited in addition to size-dependent
changes in their kinetic energy. These finite size effects depend in different
ways on the mean free path and thus provide more information on the mean free
path than corresponding liquid jet, thin film, or bulk data. The present
contribution is a proof of principle employing a simple model for the
photoemission of electrons and preliminary experimental data for potassium
chloride aerosol particles
Atomic correlations in itinerant ferromagnets: quasi-particle bands of nickel
We measure the band structure of nickel along various high-symmetry lines of
the bulk Brillouin zone with angle-resolved photoelectron spectroscopy. The
Gutzwiller theory for a nine-band Hubbard model whose tight-binding parameters
are obtained from non-magnetic density-functional theory resolves most of the
long-standing discrepancies between experiment and theory on nickel. Thereby we
support the view of itinerant ferromagnetism as induced by atomic correlations.Comment: 4 page REVTeX 4.0, one figure, one tabl
Lifetime of d-holes at Cu surfaces: Theory and experiment
We have investigated the hole dynamics at copper surfaces by high-resolution
angle-resolved photoemission experiments and many-body quasiparticle GW
calculations. Large deviations from a free-electron-like picture are observed
both in the magnitude and the energy dependence of the lifetimes, with a clear
indication that holes exhibit longer lifetimes than electrons with the same
excitation energy. Our calculations show that the small overlap of d- and
sp-states below the Fermi level is responsible for the observed enhancement.
Although there is qualitative good agreement of our theoretical predictions and
the measured lifetimes, there still exist some discrepancies pointing to the
need of a better description of the actual band structure of the solid.Comment: 15 pages, 7 figures, 1 table, to appear in Phys. Rev.
Epidemiological Characteristics of Classical Scrapie Outbreaks in 30 Sheep Flocks in the United Kingdom
Most previous analyses of scrapie outbreaks have focused on flocks run by research institutes, which may not reflect the field situation. Within this study, we attempt to rectify this deficit by describing the epidemiological characteristics of 30 sheep flocks naturally-infected with classical scrapie, and by exploring possible underlying causes of variation in the characteristics between flocks, including flock-level prion protein (PrP) genotype profile. In total, the study involved PrP genotype data for nearly 8600 animals and over 400 scrapie cases.We found that most scrapie cases were restricted to just two PrP genotypes (ARQ/VRQ and VRQ/VRQ), though two flocks had markedly different affected genotypes, despite having similar underlying genotype profiles to other flocks of the same breed; we identified differences amongst flocks in the age of cases of certain PrP genotypes; we found that the age-at-onset of clinical signs depended on peak incidence and flock type; we found evidence that purchasing infected animals is an important means of introducing scrapie to a flock; we found some evidence that flock-level PrP genotype profile and flock size account for variation in outbreak characteristics; identified seasonality in cases associated with lambing time in certain flocks; and we identified one case that was homozygous for phenylalanine at codon 141, a polymorphism associated with a very high risk of atypical scrapie, and 28 cases that were heterozygous at this codon.This paper presents the largest study to date on commercially-run sheep flocks naturally-infected with classical scrapie, involving 30 study flocks, more than 400 scrapie cases and over 8500 PrP genotypes. We show that some of the observed variation in epidemiological characteristics between farms is related to differences in their PrP genotype profile; although much remains unexplained and may instead be attributed to the stochastic nature of scrapie dynamics
Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in Acinetobacter baumannii
The reference method for cefiderocol antimicrobial susceptibility testing is broth microdilution (BMD) with iron-depleted-Mueller-Hinton (ID-MH) medium, whereas breakpoints recommended for disk diffusion (DD) are based on MH-agar plates. We aimed to compare the performance of the commercial BMD tests ComASP (Liofilchem) and UMIC (Bruker), and DD and E-test using MH- and ID-MH-agar plates with the reference BMD method using 100 carbapenem-resistant-A. baumannii isolates. Standard BMD was performed according to the EUCAST guidelines; DD and E-test were carried out using two commercial MH-agar plates (BioMérieux and Liofilchem) and an in-house ID-MH-agar plate, while ComASP and UMIC were performed according to the manufacturer's guidelines. DD performed with the ID-MH-agar plates led to a higher categorical agreement (CA, 95.1%) with standard BMD and fewer categorization errors compared to the commercial MH-agar plates (CA BioMérieux 91.1%, Liofilchem 89.2%). E-test on ID-MH-agar plates exhibited a significantly higher essential agreement (EA, 75%) with standard BMD compared to the two MH-agar plates (EA BioMérieux 57%, Liofilchem 44%), and showed a higher performance in detecting high-level resistance than ComASP and UMIC (mean log2 difference with standard BMD for resistant isolates of 0.5, 2.83, and 2.08, respectively). In conclusion, DD and E-test on ID-MH-agar plates exhibit a higher diagnostic performance than on MH-agar plates and the commercial BMD methods. Therefore, we recommend using ID-MH-agar plates for cefiderocol susceptibility testing of A. baumannii
Fluctuations and differential contraction during regeneration of Hydra vulgaris tissue toroids
We studied regenerating bilayered tissue toroids dissected from Hydra
vulgaris polyps and relate our macroscopic observations to the dynamics of
force-generating mesoscopic cytoskeletal structures. Tissue fragments undergo a
specific toroid-spheroid folding process leading to complete regeneration
towards a new organism. The time scale of folding is too fast for biochemical
signalling or morphogenetic gradients which forced us to assume purely
mechanical self-organization. The initial pattern selection dynamics was
studied by embedding toroids into hydro-gels allowing us to observe the
deformation modes over longer periods of time. We found increasing mechanical
fluctuations which break the toroidal symmetry and discuss the evolution of
their power spectra for various gel stiffnesses. Our observations are related
to single cell studies which explain the mechanical feasibility of the folding
process. In addition, we observed switching of cells from a tissue bound to a
migrating state after folding failure as well as in tissue injury.
We found a supra-cellular actin ring assembled along the toroid's inner edge.
Its contraction can lead to the observed folding dynamics as we could confirm
by finite element simulations. This actin ring in the inner cell layer is
assembled by myosin- driven length fluctuations of supra-cellular
{\alpha}-actin structures (myonemes) in the outer cell-layer.Comment: 19 pages and 8 figures, submitted to New Journal of Physic
Review of Person Re-identification Techniques
Person re-identification across different surveillance cameras with disjoint
fields of view has become one of the most interesting and challenging subjects
in the area of intelligent video surveillance. Although several methods have
been developed and proposed, certain limitations and unresolved issues remain.
In all of the existing re-identification approaches, feature vectors are
extracted from segmented still images or video frames. Different similarity or
dissimilarity measures have been applied to these vectors. Some methods have
used simple constant metrics, whereas others have utilised models to obtain
optimised metrics. Some have created models based on local colour or texture
information, and others have built models based on the gait of people. In
general, the main objective of all these approaches is to achieve a
higher-accuracy rate and lowercomputational costs. This study summarises
several developments in recent literature and discusses the various available
methods used in person re-identification. Specifically, their advantages and
disadvantages are mentioned and compared.Comment: Published 201
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