Studies on traditional Indian (turmeric) pickle as probiotic pickle for therapeutic uses in view of COVID-19 pandemic

143-152The aim of present study was to assess therapeutic potential of traditional turmeric pickle by characterizing the potential health boosting probiotics present in it and recommend it as an attractive food supplement. Probiotics TP1, TP2 and TP3 isolated from turmeric pickle had shown good antagonistic activity against all test pathogens i.e., Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus with 100% inhibition. Most potential strain amongst them was identified as Enterococcus durans TP2 strain by 16S rRNA gene technique. Further safety assessment was confirmed by evaluating haemolytic activity, DNase and gelatinase activities and all the three probiotic isolates TP1, TP2 and TP3 had shown -ve results, thereby proving their completely safe status. The strain exhibited high tolerance to acid with minimum cell survival of 39.24% after 180 min at pH 2.0, high bile salt tolerance expressed cell survival of 93.69% after 8 h at 2.0% bile salt concentration, exhibited satisfactory auto aggregation potential after 5 h i.e., higher than 40% and reveal the strong hydrophobicity. The strain TP2 also exhibited good antioxidant activity, depletion of sodium nitrite and cholesterol reduction and was found to be susceptible to most of the antibiotics used. These observations designate that Enterococcus durans TP2 [MH916769] as a good probiotic candidate for enhancing the further therapeutic potential of well known immunity booster – Turmeric in the fermented pickle, thus recommending it as highly beneficial product for health

Rationality verses irrationality in fixed dose combinations: at a tertiary teaching hospital of rural Chhattisgarh, India

Background: Rational drug prescribing can be defined as appropriate drugs prescribed in the right dose, at correct time intervals and for a sufficient duration. Irrational drug use is a common problem in many countries of the world.Methods: A prospective observational study was conducted, total 300 patients attending various outpatient departments of tertiary health care rural hospital in Rajnandgaon district were interviewed and their prescriptions were analysed.Results: Total 350 drugs were prescribed 60 (17.14%) were prescribed by generic name and the rest 290 (82.86%) were prescribed by brand name. Only 18 (5.14%) drugs were not prescribed from hospital formulary. 264 (75.43%) drugs were dispensed from hospital pharmacy. On the basis of rationality score 53% prescriptions were rational, 30% semi rational and 17% irrational.Conclusions: In a rural hospital, where hospital formulary is based on WHO Essential medicine list, hundred percent utilization of hospital pharmacy service doctors and patients would ensure rational prescribing benefits of the patients coming from rural and uneducated background

A study of adverse drug reaction profile of tuberculosis patients attending DOTS center at Dr. Bhim Rao Ambedkar memorial hospital, Raipur, Chhattisgarh, India

Background: Tuberculosis is second leading cause of death in the world. The causative organism is Mycobacterium tuberculosis. The objective was to study the adverse reactions of the patients attending the DOTS center and to assess their causality and severity of reported ADRs.Methods: Present study was a prospective observational study carried at the DOTS center of Dr. Bhim Rao Ambedkar Memorial Hospital, Raipur, Chhattisgarh, India between August 2011 to July 2012 (One year). The patients were monitored for adverse drug reactions. The assessment of ADRs were based upon the WHO assessment scale, Naranjo scale, European A.B.O scale.Results: Total number of patients attending DOTS center was 816. The number of males (428) exceeded that of females (388). Majority of patients in this study belonged to 21-30 years (26.96%) next 31-40 years (25.24%) and 41-50 years (16.5%) of age group. Prevalence of ADRs were more in males (57%) than in females 32 (43%). Majority of ADRs reported were moderate 33 (35.22%) followed by 29 (46,77%) were mild, no severe ADRs reported. According to severity of ADRs seen were gastritis 28 (45%) followed by 10 (16% ) rashes , 10 (16,12%) of arthralgia, 3 (4.83%) of hepatitis, 6 (9.7%) of peripheral neuropathy, 2 (3%) onsets of ADRs after starting anti-tubercular drug were 12 (19.35%) in 0-1 week followed by 19 (30%) ADRs showed onset in 1-2 week and 2-3 week, 8 (13%) in 3-4 week 3 (5%) in 4-5 week and 1 (2%) in 5-6 week.Conclusions: The casual link between the ADRs and the suspected anti-tubercular drug by Naranjo scale definitely relationship was established between the anti-tubercular drug and ADRs in 7 (11.25%) patient while 22 (35.45%) probable and 33 (53.22%) ADRs were categorized as possible

Variation in mitochondrial function in hypoxia-sensitive and hypoxia-tolerant human glioma cells

We have shown previously that human glioblastoma multiforme cells vary in their ability to survive under hypoxic conditions. Under oxygen limiting conditions, hypoxia-tolerant cells decrease their oxygen consumption rate whereas hypoxia-sensitive cells continue to consume oxygen at a relatively steady rate until the oxygen supply becomes exhausted. We now show that hypoxia-tolerant and hypoxia-sensitive cells exhibit distinct patterns of mitochondrial function in response to hypoxic challenge. Hypoxia-tolerant cell lines retain stable mitochondrial membrane potential and ATP concentration when incubated under oxygen limiting conditions. In addition, hypoxia-tolerant cell lines are consistently more sensitive to a wide spectrum of inhibitors of mitochondrial function than are hypoxia-sensitive cells. In contrast, the hypoxia-sensitive cells are unable to maintain stable mitochondrial membrane potential and ATP levels when incubated at reduced oxygen tension. These results demonstrate significant differences in the mitochondrial function between these two phenotypes and reinforce previous data that suggest a regulatory role for mitochondria in the development of hypoxia tolerance

Assessment of knowledge of pharmacotherapeutics amongst medical undergraduates of a tertiary care teaching hospital of Chhattisgarh, India: a questionnaire based study

Background: Knowledge of pharmacology forms the basis of rational pharmacotherapy practice. Teaching the medical students about systematic application of pharmacology in patients’ care forms an essential component. It facilitates the medical students to develop a methodical approach in solving patients’ clinical problems.Methods: A questionnaire-based study was conducted, at Government Medical College Rajnandgaon involving second year MBBS students. Total 100 students participated in the study.Results: Hundred percent of the students responded that pharmacology was presently taught to them, seventy nine percent of them agreed with the fact that pharmacology was preferred to pass the MBBS, eighty eight percent of them responded that in pharmacokinetics was the least preferred topic, ninety seven of them were not aware of the essential drug list. Their suggestions regarding the change in teaching methodology was recorded.Conclusions: This study concludes that efforts are needed to develop a curriculum that encompasses important aspects of clinical pharmacology and therapeutics along with incorporation of the useful suggestions by the undergraduate students

Oxidation of thiocarbamyls with lead tetraacetate

1178-118

Irrigated pinto bean crop stress and yield assessment using ground based low altitude remote sensing technology

The pinto bean is one of widely consumed legume crop that constitutes over 42% of the U.S dry bean production. However, limited studies have been conducted in past to assess its quantitative and qualitative yield potentials. Emerging remote sensing technologies can help in such assessment. Therefore, this study evaluates the role of ground-based multispectral imagery derived vegetation indices (VIs) for irrigated the pinto bean stress and yield assessments. Studied were eight cultivars of the pinto bean grown under conventional and strip tillage treatments and irrigated at 52% and 100% of required evapotranspiration. Imagery data was acquired using a five-band multispectral imager at early, mid and late growth stages. Commonly used 25 broadband VIs were derived to capture crop stress traits and yield potential. Principal component analysis and Spearman’s rank correlation tests were conducted to identify key VIs and their correlation (rs) with abiotic stress at each growth stage. Transformed difference vegetation index, nonlinear vegetation index (NLI), modified NLI and infrared percentage vegetation index (IPVI) were consistent in accounting the stress response and crop yield at all growth stages (rs \u3e 0.60, coefficient of determination (R2): 0.50–0.56, P \u3c 0.05). Ten other VIs significantly accounted for crop stress at early and late stages. Overall, identified key VIs may be helpful to growers for precise crop management decision making and breeders for crop stress response and yield assessments

Sistema Solar: Planetas Clássicos

O conhecimento curricular de Astronomia para surdos, não pode ser empobrecido, subtraído, fragmentado, mas sim formulado para corresponder a sua identidade de cognição, sem distanciar-se, porém, do direito inalienável a tudo que devem conhecer. Métodos de ensino não podem ser únicos para todos e, um sistema educacional que não revela estas diferenças está fadado em provocar a exclusão destes educandos por considerá-los inaptos, intelectualmente. Sendo assim, ao organizar o conteúdo que será trabalhado em sala de aula, o professor terá sempre em mente o tema Sistema Solar /Planetas Clássicos. Este tema está diretamente ligado a outros temas, permitindo ao aluno surdo fazer parte desse todo tão complexo que é o Universo em que vivemo