Hydrodynamics and mass transfer in straight fiber bundles with non-uniform porosity

The present study investigates the effects of non-uniformity in a bundle's porosity by considering a model channel made up of "dense" (low porosity) and "loose" (high porosity) regions. In a first, simplified, approach these regions are treated as non-interacting porous media and previously obtained computational results are used for the Darcy permeability and the Sherwood number. In a second, and more complete, approach 3-D CFD simulations are conducted for a checkerboard arrangement of alternately "dense" and "loose" regions with square-arrayed fibers, accounting for entry effects and for interactions between regions. Non-uniformity causes a significant increase of the permeability and a strong reduction of the Sherwood number. These effects are larger, approaching those obtained for non-interacting regions, if the regions' length scale is large. The attainment of fully developed conditions is greatly shifted forward in non-uniform bundles and the mass transfer development length may largely exceed the physical length of most hollow-fiber devices

Performance Comparison of Alternative Hollow-Fiber Modules for Hemodialysis by Means of a CFD-Based Model

Commercial hemodialyzers are hollow-fiber cylindrical modules with dimensions and inlet– outlet configurations dictated mostly by practice. However, alternative configurations are possible, and one may ask how they would behave in terms of performance. In principle, it would be possible to depart from the standard counter-flow design, while still keeping high clearance values, thanks to the increase in the shell-side Sherwood number (Sh) due to the cross-flow. To elucidate these aspects, a previously developed computational model was used in which blood and dialysate are treated as flowing through two interpenetrating porous media. Measured Darcy permeabilities and mass transfer coefficients derived from theoretical arguments and CFD simulations conducted at unit-cell scale were used. Blood and dialysate were alternately simulated via an iterative strategy, while appropriate source terms accounted for water and solute exchanges. Several module configurations sharing the same membrane area, but differing in overall geometry and inlet–outlet arrangement, were simulated, including a commercial unit. Although the shell-side Sherwood number increased in almost all the alternative configurations (from 14 to 25 in the best case), none of them outperformed in terms of clearance the commercial one, approaching the latter (257 vs. 255 mL/min) only in the best case. These findings confirmed the effectiveness of the established commercial module design for the currently available membrane properties

Influence of bundle porosity on shell-side hydrodynamics and mass transfer in regular fiber arrays: A computational study

CFD predictions of the effects of a fiber bundle porosity on shell-side hydrodynamics and mass transfer under conditions of steady laminar flow were obtained. Fluid was assumed to flow around regular hexag-onal or square arrays of cylindrical fibers of different pitch to diameter ratios, yielding bundle porosities ranging from the theoretical minimum up to similar to 1. A large number of axial, transverse and mixed flow combinations were simulated by letting the axial and transverse flow Reynolds numbers and the trans-verse flow attack angle vary. Both fully developed and developing conditions (entrance effects) were con-sidered. The continuity and momentum equations, along with a transport equation for the concentra-tion of a high-Schmidt number solute, were solved by a finite volume CFD code. Fully developed condi-tions were simulated by the well-established "unit cell" approach, in which the computational domain is two-dimensional and includes a single fiber with the associated fluid, periodic boundary conditions are imposed between all opposite sides and compensative terms are introduced to account for large-scale longitudinal or transversal gradients. Developing flow was studied by using a fully three-dimensional computational domain. Predictions were validated against experimental, computational and analytic liter-ature results. The simulations showed that lattices with different porosities exhibit a qualitatively similar behavior, but differ significantly in important quantities such as the Darcy permeability, the Sherwood number and the hydrodynamic and mass transfer development length

A pilot-plant for the selective recovery of magnesium and calcium from waste brines

The problem of brines disposal has raised great interest towards new strategies for their valorisation through the recovery of minerals or energy. As an example, the spent brine from ion exchange resins regeneration is often discharged into rivers or lakes, thus impacting on the process sustainability. However, such brines can be effectively reconcentrated, after removal of bivalent cations, and reused for the resins regeneration. This work focuses on developing and testing a pilot plant for selective recovery of magnesium and calcium from spent brines exploiting a novel proprietary crystallization unit. This is part of a larger treatment chain for the complete regeneration of the brine, developed within the EU-funded ZERO BRINE project. The pilot crystallizer was tested with the retentate of the nanofiltration unit processing the spent brine from the industrial water production plant of Evides Industriewater B.V. (Rotterdam, The Netherlands). Magnesium and calcium hydroxide were selectively precipitated by adding alkaline solution in two consecutive steps and controlling reaction pH. Performance was assessed in terms of recovery efficiency and purity of produced crystals, observing in most investigated cases a recovery of about 100% and 97% and a purity above 90% and 96%, for magnesium and calcium hydroxide, respectively

Cloning of the Repertoire of Individual Plasmodium falciparum var Genes Using Transformation Associated Recombination (TAR)

One of the major virulence factors of the malaria causing parasite is the Plasmodium falciparum encoded erythrocyte membrane protein 1 (PfEMP1). It is translocated to It the membrane of infected erythrocytes and expressed from approximately 60 var genes in a mutually exclusive manner. Switching of var genes allows the parasite to alter functional and antigenic properties of infected erythrocytes, to escape the immune defense and to establish chronic infections. We have developed an efficient method for isolating VAR genes from telomeric and other genome locations by adapting transformation-associated recombination (TAR) cloning, which can then be analyzed and sequenced. For this purpose, three plasmids each containing a homologous sequence representing the upstream regions of the group A, B, and C var genes and a sequence homologous to the conserved acidic terminal segment (ATS) of var genes were generated. Co-transfection with P. falciparum strain ITG2F6 genomic DNA in yeast cells yielded 200 TAR clones. The relative frequencies of clones from each group were not biased. Clones were screened by PCR, as well as Southern blotting, which revealed clones missed by PCR due to sequence mismatches with the primers. Selected clones were transformed into E. coli and further analyzed by RFLP and end sequencing. Physical analysis of 36 clones revealed 27 distinct types potentially representing 50% of the var gene repertoire. Three clones were selected for sequencing and assembled into single var gene containing contigs. This study demonstrates that it is possible to rapidly obtain the repertoire of var genes from P. falciparum within a single set of cloning experiments. This technique can be applied to individual isolates which will provide a detailed picture of the diversity of var genes in the field. This is a powerful tool to overcome the obstacles with cloning and assembly of multi-gene families by simultaneously cloning each member

Refined human artificial chromosome vectors for gene therapy and animal transgenesis

Human artificial chromosomes (HACs) have several advantages as gene therapy vectors, including stable episomal maintenance, and the ability to carry large gene inserts. We previously developed HAC vectors from the normal human chromosomes using a chromosome engineering technique. However, endogenous genes were remained in these HACs, limiting their therapeutic applications. In this study, we refined a HAC vector without endogenous genes from human chromosome 21 in homologous recombination-proficient chicken DT40 cells. The HAC was physically characterized using a transformation-associated recombination (TAR) cloning strategy followed by sequencing of TAR-bacterial artificial chromosome clones. No endogenous genes were remained in the HAC. We demonstrated that any desired gene can be cloned into the HAC using the Cre-loxP system in Chinese hamster ovary cells, or a homologous recombination system in DT40 cells. The HAC can be efficiently transferred to other type of cells including mouse ES cells via microcell-mediated chromosome transfer. The transferred HAC was stably maintained in vitro and in vivo. Furthermore, tumor cells containing a HAC carrying the suicide gene, herpes simplex virus thymidine kinase (HSV-TK), were selectively killed by ganciclovir in vitro and in vivo. Thus, this novel HAC vector may be useful not only for gene and cell therapy, but also for animal transgenesis

Transgenic Expression of Nonclassically Secreted FGF Suppresses Kidney Repair

FGF1 is a signal peptide-less nonclassically released growth factor that is involved in angiogenesis, tissue repair, inflammation, and carcinogenesis. The effects of nonclassical FGF export in vivo are not sufficiently studied. We produced transgenic mice expressing FGF1 in endothelial cells (EC), which allowed the detection of FGF1 export to the vasculature, and studied the efficiency of postischemic kidney repair in these animals. Although FGF1 transgenic mice had a normal phenotype with unperturbed kidney structure, they showed a severely inhibited kidney repair after unilateral ischemia/reperfusion. This was manifested by a strong decrease of postischemic kidney size and weight, whereas the undamaged contralateral kidney exhibited an enhanced compensatory size increase. In addition, the postischemic kidneys of transgenic mice were characterized by hyperplasia of interstitial cells, paucity of epithelial tubular structures, increase of the areas occupied by connective tissue, and neutrophil and macrophage infiltration. The continuous treatment of transgenic mice with the cell membrane stabilizer, taurine, inhibited nonclassical FGF1 export and significantly rescued postischemic kidney repair. It was also found that similar to EC, the transgenic expression of FGF1 in monocytes and macrophages suppresses kidney repair. We suggest that nonclassical export may be used as a target for the treatment of pathologies involving signal peptide-less FGFs

Evidence for long-range glycosyl transfer reactions in the gas phase

AbstractA long-range glycosyl transfer reaction was observed in the collision-induced dissociation Fourier transform (CID FT) mass spectra of benzylamine-labeled and 9-aminofluorene-labeled lacto-N-fucopentaose I (LNFP I) and lacto-N-difucohexaose I (LNDFH I). The transfer reaction was observed for the protonated molecules but not for the sodiated molecules. The long-range glycosyl transfer reaction involved preferentially one of the two L-fucose units in labeled LNDFH I. CID experiments with labeled LNFP I and labeled LNFP II determined the fucose with the greatest propensity for migration. Further experiments were performed to determine the final destination of the migrating fucose. Molecular modeling supported the experiments and reaction mechanisms are proposed