Arf6 determines tissue architecture by stabilizing intercellular adhesion

Correct cell shape is indispensable for tissue architecture, with cell shape being determined by cortical actin and surface adhesion. The role of adhesion in remodeling tissue is to counteract the deformation of cells by force, resulting from actomyosin contractility, and to maintain tissue integrity. The dynamics of this adhesion is critical to the processes of cell shape formation and maintenance. Here, we show that the trafficking molecule, Arf6, has a direct impact on cell elongation, by acting to stabilize E-cadherin-based adhesion complexes at the cell surface, in addition to its canonical role in endocytosis. We demonstrate that these functions of Arf6 are dependent on the molecule Flotillin1, which recruits Arf6 to the plasma membrane. Our data suggest that Arf6 and Flotillin1 operate in a pathway distinct from clathrin-mediated endocytosis. Altogether, we demonstrate that Arf6- and Flotillin1-dependent regulation of the dynamics of cell adhesion contributes to molding tissue in vivo

The Technique of Fitball Aerobics in Physical Culture Classes for Female Students

The article sheds some light upon the technique of using fitball, a type of health-improving aerobics. The technique of fitball aerobics in classes touches upon physical education of the first and the second year female students. Development and implementation of the technique includes three stages. The first stage is the organization, methodological approaches, the second - the introduction into the educational process of students, the third is the method testing in a pedagogical experiment. The experimental material confirms that the methodology of fitball aerobics is effective for the development of motor characteristics, aims at the harmonious development of individual students, helps to improve overall fitness and is aimed at the preservation of health. In addition, the results suggest a fundamentally new approach to organizing and conducting of physical training in higher educational institutions. The technique of fitball is an alternative to the traditional means of physical education used in the classroom for physical education in higher educational institutions that do not satisfy the needs of female students. Exercises developed by the author, the use of a line method when performing exercises in the main classes according to musical accompaniment, allows to create a positive emotional background on lessons and a motivation to continue this type of exercises after graduation from the university.В статье рассматривается методика использования фитбола, одного из видов оздоровительной аэробики. Разработана методика применения фитбол-аэробики на занятиях по физической культуре со студентками первого, второго курсов. Разработка и внедрение методики предусматривает три этапа. Первый этап - организация, методологические подходы, второй - внедрение в учебный процесс студенток, третий апробация методики в условиях педагогического эксперимента. Представленные экспериментальные материалы подтверждают, что методика фитбол- аэробики является эффективной для развития двигательных качеств, направлена на гармоничное развитие личности студенток, способствует повышению уровня общей подготовленности и направлена на сохранение здоровья. Кроме того, полученные результаты указывают на принципиально новый подход к организации и проведению занятий по физической культуре в высшем образовательном учреждении. Методика фитбола является альтернативной по отношению к традиционным средствам физического воспитания, используемых на занятиях по физической культуре в высших учебных заведениях, которые не удовлетворяют запросы студенток. Разработанные автором комплексы упражнений, применение поточного метода при выполнении упражнений в основной части занятия с музыкальным сопровождением, позволяет на занятии создать положительный эмоциональный фон и сформировать мотивацию к продолжению занятий после окончания вуза

Photoionization and transient Wannier-Stark ladder in silicon: First-principles simulations versus Keldysh theory

Nonlinear photoionization of dielectrics and semiconductors is widely treated in the framework of the Keldysh theory whose validity is limited to photon energies that are small compared to the band gap and relatively low laser intensities. The time-dependent density functional theory (TDDFT) simulations, which are free of these limitations, enable one to gain insight into nonequilibrium dynamics of the electronic structure. Here we apply TDDFT to investigate the photoionization of silicon crystal by ultrashort laser pulses in a wide range of laser wavelengths and intensities and compare the results with predictions of the Keldysh theory. Photoionization rates derived from the simulations considerably exceed the data obtained with the Keldysh theory within the validity range of the latter. Possible reasons for the discrepancy are discussed and we provide fundamental data on the photoionization rates beyond the limits of the Keldysh theory. By investigating the features of the Stark shift as a function of photon energy and laser field strength, a manifestation of the transient Wannier-Stark ladder states is revealed, which become blurred with increasing laser field strength. Finally, it is shown that the TDDFT simulations can potentially provide reliable data on the electron damping time that is of high importance for large-scale modeling

ІННОВАЦІЙНІ ТЕХНОЛОГІЇ НАВЧАННЯ ПРИ ВИКЛАДАННІ ДИСЦИПЛІН ХІРУРГІЧНОГО ЦИКЛУ

     In the article described surgery subject   study experience in theConstantinemedical school for prepare qualified nurses.У статті описано досвід викладання дисциплін хірургічного профілю у Костянтинівському медичному училищі для підготовки висококваліфікованого середнього медичного персоналу

Age peculiarities of the pro-inflammatory component of cytokine profile in chronic odontogenic infection affected by polymorbid pathology

This study aimed to study the features of pro-inflammatory component of the cytokine profile in chronic odontogenic infection affected by polymorbid patholog

Comparative analysis of indicators of physical development and physical fitness of university students of various educational physical culture groups during the СOVID-19 pandemic

The purpose of the study is a comparative analysis of some indicators of physical development and physical fitness of university students of various physical education groups before and after the restrictions of COVID-19. The article presents the experience of monitoring the physical development of higher school students by the Laboratory of Sports and Tourism (LS&T) of the Institute of Physical Culture of Sports and Tourism (IFKSiT) of the Siberian Federal University (SFU).The indicators of physical development and physical fitness of students of 1–3 courses, boys and girls aged 17–22 years of physical education groups: aerobics, basketball and special medical group (SMG) of various institutes of SFU are represented, the analysis shows that these indicators in the groups under study during the predominance of distance learning are insignificantly reduced. The most reduced indicator is the relative strength indicator. The lowest indicators of physical development and physical fitness for all the considered parameters are characteristic of SMG students. The least reduced indicators of physical development and physical fitness were characteristic of the representatives of the aerobics groups, while the flexibility indicator was improved. The current unfavorable epidemiological situation has confirmed the need for the development of various systems for monitoring indicators of physical and functional conditions, physical development, health and physical fitness for higher education, both in the context of the implementation of the subject of physical culture in the educational process and correction of developmental influences during classes, and in the formation of a health-preserving environment of educational institutions

Cytotoxic properties of the complex of the antitumor antibiotic bleomycetin and Bacillus intermedius ribonuclease

It was shown possible to change the cytotoxic properties of the antitumor antibiotic bleomycetin by its binding to Bacillus intermedius RNAse. The complexing lowered the antibiotic effect on DNA in the cells of the human amnion. At the same time the experiments with human red blood cells indicated that RNAse of B.intermedius in complex with bleomycetin - Fe (II) increased the antibiotic capacity for the cell membrane break down

Innovational methods of development of intellectual labor for economy’s security

The notion “development of intellectual labor for the purpose of economy’s security” is viewed as development of society’s intellectual potential that includes the protected socio-economic information, developed by a person or a group of persons. The social factors that reduce economic security and their consequences in economy are given, namely: negative dynamics of implementing new progressive technologies into production, insufficient coordination of work in the sphere of innovational development, etc. The forms of intellectual development of human resources (intellectual development of personality, control over intellectual information) are offered, which bring the country’s economy to competitiveness and security. The traditional and innovational methods of intellectual labor development are studied (studying in universities and colleges, increase of personnel’s qualification in view of academic degrees (Ph.D., doctor of economics), as well as receipt of economic information through Internet resources, scientific publication, statistical information, etc.), as well as the methods of development of IT services and methods of prevention of intellectual diversions and violation of information confidentiality. It is offered to implement the program of equal initial possibilities for intellectual development of human resources in view of access to higher education, creative activities, as well as legal protection for everyone, etc. Analysis of implementation of innovational methods of intellectual labor development supposes planning activities in view of development of intellectual labor for the purpose of the region’s economy’s security.peer-reviewe

Modification of Bacillus intermedius ribonuclease by urea derivatives

The results of the 'Bacillus intermedius' RNAase modification by urea derivatives are presented. The modifiers synthesized for that purpose were the following: N-(4-chlorbenzoyl)-N'-benzolsulfonyl urea (M 1) and N-(4-chlorbenzoyl)carbomoyl-ε-aminocapronic acid (M 2). It was shown that RNAase modified by M 2 stimulated the cell general metabolism by the test of the cell absorption of neutral red and had a more marked ability to disrupt RNA of the cell plasmatic membranes in comparison to that of the native enzyme

Course of acute coronary syndrome in patients with fraillty

The goal of this study was to study the course and outcome of acute coronary syndrome with ST-segment elevation and without ST-segment elevation in patients of older age groups with frailt