13 research outputs found
FEATURES OF THE CLINICAL SIGNIFICANCE OF POLYMORPHIC VARIANTS OF ENOS AND AGTR2 GENES IN PATIENTS WITH CAD
Coronary heart disease (CHD) is a major cause of mortality. Morphological substrate of CHD in most cases is atherosclerosis, which is based on structural genes polymorphism eNOS and AGTR2. The aim of the study was to study the prevalence of eNOS and AGTR2 genes in patients with coronary artery disease and the association of these genes with coronary heart disease. The study involved 187 patients aged 36 to 86 years (62,2Β±11,2) with different forms of CHD: stable and unstable angina, myocardial infarction and 45 people without CHD. Determination of gene polymorphisms was performed by real-time PCR analyzer of nucleic acids IQ 5 Bio-Rad. Statistical analysis was performed using Statistica 10.0. The study revealed a significant difference between the incidence of homozygous AA allelic variant gene AGTR2 group of patients with myocardial infarction and the comparison group; polymorphic variant AA AGTR2 gene is associated with earlier onset of coronary artery disease; It found that carriers of the polymorphic variant gene GA AGTR2 beginning statistically CHD occurred significantly later than in carriers of alleles GG and AA; age CHD debut TT allele carriers of the eNOS gene is associated with an earlier onset of the disease and statistically significantly different from the age of first CHD in carriers of alleles of polymorphic variants of GG and GT; revealed a positive correlation between the polymorphic allele AGTR2 geneΒ with the presence of arterial hypertension in patients with coronary artery disease; It determined that the T allele carriers of the polymorphic gene eNOS is associated more early onset of hypertension, found the association of the polymorphic allele gene AGTR2 the need to use higher doses of ACE inhibitor β perindopril
MSMEG_2731, an Uncharacterized Nucleic Acid Binding Protein from Mycobacterium smegmatis, Physically Interacts with RPS1
While the M. smegmatis genome has been sequenced, only a small portion of the genes have been characterized experimentally. Here, we purify and characterize MSMEG_2731, a conserved hypothetical alanine and arginine rich M. smegmatis protein. Using ultracentrifugation, we show that MSMEG_2731 is a monomer in vitro. MSMEG_2731 exists at a steady level throughout the M. smegmatis life-cycle. Combining results from pull-down techniques and LS-MS/MS, we show that MSMEG_2731 interacts with ribosomal protein S1. The existence of this interaction was confirmed by co-immunoprecipitation. We also show that MSMEG_2731 can bind ssDNA, dsDNA and RNA in vitro. Based on the interactions of MSMEG_2731 with RPS1 and RNA, we propose that MSMEG_2731 is involved in the transcription-translation process in vivo
Mycobacterium tuberculosis ribosomal protein S1 (RpsA) and variants with truncated C-terminal end show absence of interaction with pyrazinoic acid.
Pyrazinamide (PZA) is an antibiotic used in first- and second-line tuberculosis treatment regimens. Approximately 50% of multidrug-resistant tuberculosis and over 90% of extensively drug-resistant tuberculosis strains are also PZA resistant. Despite the key role played by PZA, its mechanisms of action are not yet fully understood. It has been postulated that pyrazinoic acid (POA), the hydrolyzed product of PZA, could inhibit trans-translation by binding to Ribosomal protein S1 (RpsA) and competing with tmRNA, the natural cofactor of RpsA. Subsequent data, however, indicate that these early findings resulted from experimental artifact. Hence, in this study we assess the capacity of POA to compete with tmRNA for RpsA. We evaluated RpsA wild type (WT), RpsA βA438, and RpsA βA438 variants with truncations towards the carboxy terminal end. Interactions were measured using Nuclear Magnetic Resonance spectroscopy (NMR), Isothermal Titration Calorimetry (ITC), Microscale Thermophoresis (MST), and Electrophoretic Mobility Shift Assay (EMSA). We found no measurable binding between POA and RpsA (WT or variants). This suggests that RpsA may not be involved in the mechanism of action of PZA in Mycobacterium tuberculosis, as previously thought. Interactions observed between tmRNA and RpsA WT, RpsA βA438, and each of the truncated variants of RpsA βA438, are reported
[Positional cloning of chromosomal regions controlling QTLs in chickens] ΠΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ°ΠΉΠΎΠ½ΠΎΠ² Ρ ΡΠΎΠΌΠΎΡΠΎΠΌ, ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠΈΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ Ρ ΠΊΡΡ
Π‘Π°Π·Π°Π½ΠΎΠ² Π.Π.; Π¦Π°ΡΠ΅Π²Π° Π.Π.; Π‘ΠΌΠΈΡΠ½ΠΎΠ² Π.Π€.; ΠΠ°ΡΠ΄Π΅ΡΠΊΠ° Π.; ΠΠΎΡΡΠ°ΠΊ Π.; Π―ΡΠ°ΠΊ Π.; Π ΠΎΠΌΠ°Π½ΠΎΠ² M.Π.
ΠΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²ΠΎ Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎ ΡΠ΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π΄ΠΎΠΌΠ°ΡΠ½ΠΈΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΈΠΌΠ΅ΡΡ ΡΠ»ΠΎΠΆΠ½ΡΠΉ ΠΏΠΎΠ»ΠΈΠ³Π΅Π½Π½ΡΠΉ ΡΠΈΠΏ Π½Π°ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΡΡ ΠΌΠ½ΠΎΠ³ΠΈΠΌΠΈ Π³Π΅Π½Π°ΠΌΠΈ, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΠΌΠΈ Π² Π»ΠΎΠΊΡΡΠ°Ρ
QTL (quantitative trait loci). ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠΉ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠΉ Π°ΡΡ
ΠΈΡΠ΅ΠΊΡΡΡΡ QTL ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅Ρ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ Ρ ΡΠΎΡΠΊΠΈ Π·ΡΠ΅Π½ΠΈΡ ΠΎΠ±ΡΠ΅ΠΉ Π³Π΅Π½Π΅ΡΠΈΠΊΠΈ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π΄Π°Π½Π½ΡΠ΅ ΠΎ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ
ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ
ΠΈΠ· ΡΠ°ΠΉΠΎΠ½ΠΎΠ² QTL ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π² ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΆΠΈΠ²ΠΎΡΠ½ΠΎΠ²ΠΎΠ΄ΡΡΠ²Π΅ Π΄Π»Ρ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² (marker assisted selection, MAS).
Π Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ Π½Π°ΠΌΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΡΠ΄ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² ΠΏΠΎ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΌΡ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π΄Π²ΡΡ
ΡΠ°ΠΉΠΎΠ½ΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 4 Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ QTL ΡΠΎΠ»ΡΠΈΠ½Ρ ΡΠΊΠΎΡΠ»ΡΠΏΡ Π½Π° 53 Π½Π΅Π΄Π΅Π»ΠΈ ΠΆΠΈΠ·Π½ΠΈ (ST53) ΠΈ ΠΌΠ°ΡΡΡ Π±Π΅Π»ΠΊΠ° Π² ΡΠΉΡΠ΅ Π½Π° 33 Π½Π΅Π΄Π΅Π»Π΅ (AW33). Π£ΠΊΠ°Π·Π°Π½Π½ΡΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ ΡΠ°Π·Π»ΠΈΡΠ°ΡΡΡΡ Ρ Π΄Π²ΡΡ
Π»ΠΈΠ½ΠΈΠΉ ΠΊΡΡ (ΠΏΠΎΠ»ΡΡΠΊΠ°Ρ Π·Π΅Π»Π΅Π½ΠΎΠ½ΠΎΠ³Π°Ρ ΠΈ ΡΠΎΠ΄-Π°ΠΉΠ»Π΅Π½Π΄) Π½Π° 3,3% ΠΈ 7,5%, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ° AW33 Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌ MCW170 (Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ°ΡΡΡΠΎΡΠ½ΠΈΠ΅ 1ΡΠ) ΠΈ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ ΠΏΠΎΠ»Π½ΠΎΠ΅ ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ QTL ST53 Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌ MCW114. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π±Π°Π· Π΄Π°Π½Π½ΡΡ
ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠΈ ΠΠ½ΡΠ΅ΡΠ½Π΅Ρ ΠΏΠΎΠΊΠ°Π·Π°Π½Π° Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ° AW33 Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»Π°, ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ Π»ΠΎΠΊΡΡΠ°ΠΌΠΈ MCW0170 ΠΈ LEI0081, ΠΈ QTL ST53 Π²Π½ΡΡΡΠΈ ΡΠ°ΠΉΠΎΠ½Π° Ρ Π³ΡΠ°Π½ΠΈΡΠ°ΠΌΠΈ MCW0114 ΠΈ ADL0241. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ Π³ΡΠΈΠ΄ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ BAC-Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ ΠΊΡΡΠΈΡΡ 031-JF256-BI (http://hbz.tamu.edu) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΠΠ-Π·ΠΎΠ½Π΄ΠΎΠ² ΠΌΠ΅ΡΠ΅Π½ΡΡ
-32P-dCTP ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠΎΠ² MCW0170, LEI0081, MCW0114 ΠΈ ADL0241. ΠΡΠ°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΏΡΠΈ ΠΏΠΎΠΌΠΎΡΠΈ ΡΠΊΠ°Π½Π΅ΡΠ° FX-scan ΠΈ ΠΏΠ°ΠΊΠ΅ΡΠ° ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΡΡ
ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌ Quantity One. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΊΠΎΠΎΡΠ΄ΠΈΠ½Π°ΡΡ Π΄Π²Π°Π΄ΡΠ°ΡΠΈ ΠΊΠ»ΠΎΠ½ΠΎΠ², ΠΈΠΌΠ΅ΡΡΠΈΡ
Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΠΠ Π²ΡΡΠ°Π²ΠΊΠΈ Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ Π»ΠΎΠΊΡΡΠ°ΠΌΠΈ MCW0170, LEI0081, MCW0114 ΠΈ ADL0241.
ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡ Β«Π·Π°ΡΠΊΠΎΡΠΈΡΡΒ» ΡΠ°ΠΉΠΎΠ½Ρ QTL Π½Π° Π΄Π΅ΡΠ°Π»ΡΠ½ΠΎ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΡ
ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΡ
Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠ°ΡΡΠ°Ρ
Β«ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ-ΠΌΡΡΡΒ» Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ, Π²ΡΡΠ²ΠΈΡΡ Π³Π΅Π½Ρ, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΠ΅ Π·Π° QTL ΡΠΎΠ»ΡΠΈΠ½Ρ ΡΠΊΠΎΡΠ»ΡΠΏΡ ΠΈ ΠΌΠ°ΡΡΡ Π±Π΅Π»ΠΊΠ° Π² ΡΠΉΡΠ΅. ( ΠΠ°Π½Π΄ΠΈΠ΄Π°ΡΡΠΊΠΈΠ΅ ΠΏΡΠΎΠ΅ΠΊΡΡ.
[Positional cloning of quantitative trait loci in the domestic fowl] ΠΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π»ΠΎΠΊΡΡΠΎΠ² ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Ρ Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ
ΠΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²ΠΎ Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎ ΡΠ΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π΄ΠΎΠΌΠ°ΡΠ½ΠΈΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΈΠΌΠ΅ΡΡ ΡΠ»ΠΎΠΆΠ½ΡΠΉ ΠΏΠΎΠ»ΠΈΠ³Π΅Π½Π½ΡΠΉ ΡΠΈΠΏ Π½Π°ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΡΡ ΠΌΠ½ΠΎΠ³ΠΈΠΌΠΈ Π³Π΅Π½Π°ΠΌΠΈ, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΠΌΠΈ Π² Π»ΠΎΠΊΡΡΠ°Ρ
QTL (quantitative trait loci). ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΡΠ΄ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² ΠΏΠΎ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΌΡ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π΄Π²ΡΡ
ΡΠ°ΠΉΠΎΠ½ΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 4 Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ QTL ΡΠΎΠ»ΡΠΈΠ½Ρ ΡΠΊΠΎΡΠ»ΡΠΏΡ Π½Π° 53 Π½Π΅Π΄Π΅Π»ΠΈ ΠΆΠΈΠ·Π½ΠΈ (ST53) ΠΈ ΠΌΠ°ΡΡΡ Π±Π΅Π»ΠΊΠ° Π² ΡΠΉΡΠ΅ Π½Π° 33 Π½Π΅Π΄Π΅Π»Π΅ (AW33). Π£ΠΊΠ°Π·Π°Π½Π½ΡΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ ΡΠ°Π·Π»ΠΈΡΠ°ΡΡΡΡ Ρ Π΄Π²ΡΡ
Π»ΠΈΠ½ΠΈΠΉ ΠΊΡΡ (ΠΏΠΎΠ»ΡΡΠΊΠ°Ρ Π·Π΅Π»Π΅Π½ΠΎΠ½ΠΎΠ³Π°Ρ ΠΈ ΡΠΎΠ΄-Π°ΠΉΠ»Π΅Π½Π΄) Π½Π° 3,3% ΠΈ 7,5%, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΊΠΎΡΠ΅Π³ΡΠ΅Π³Π°ΡΠΈΠΈ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΡ
ΠΠΠ-ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΌΠ°ΡΠΊΠ΅ΡΡ ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»ΠΎΠ² Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ°ΡΡΡ (ΡΠ°ΠΉΠΎΠ½ΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ), ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠΈΡ
QTL AW33 ΠΈ ST53. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π±Π°Π· Π΄Π°Π½Π½ΡΡ
ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠΈ ΠΠ½ΡΠ΅ΡΠ½Π΅Ρ ΠΏΠΎΠΊΠ°Π·Π°Π½Π° Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ° AW33 Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΠΈΠ½ΡΠ΅ΡΠ²Π°Π»Π°, ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ Π»ΠΎΠΊΡΡΠ°ΠΌΠΈ MCW0170 ΠΈ LEI0081, ΠΈ QTL ST53 Π²Π½ΡΡΡΠΈ ΡΠ°ΠΉΠΎΠ½Π° Ρ Π³ΡΠ°Π½ΠΈΡΠ°ΠΌΠΈ MCW0114 ΠΈ ADL0241. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΠΊΠΎΠΎΡΠ΄ΠΈΠ½Π°ΡΡ Π΄Π²Π°Π΄ΡΠ°ΡΠΈ ΠΊΠ»ΠΎΠ½ΠΎΠ², ΠΈΠΌΠ΅ΡΡΠΈΡ
Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ ΠΠΠ Π²ΡΡΠ°Π²ΠΊΠΈ Ρ ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠΌΠΈ Π»ΠΎΠΊΡΡΠ°ΠΌΠΈ MCW0170, LEI0081, MCW0114 ΠΈ ADL0241. ΠΠ΅ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π°ΡΡΠ΅Π½ΡΠΈΡΠ½ΠΎΡΡΠΈ ΠΊΠ»ΠΎΠ½ΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π΄Π»Ρ ΡΡΠ΅Ρ
ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΡ
Π»ΠΎΠΊΡΡΠΎΠ² (MCW0170, MCW0114 ΠΈ ADL0241) ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»Π° ΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠ΅ Π΄Π²ΡΡ
BAC-ΠΊΠ»ΠΎΠ½ΠΎΠ² Π»ΠΎΠΊΡΡΡ MCW0170 (QTL AW33), Π΄Π΅Π²ΡΡΠΈ β Π»ΠΎΠΊΡΡΡ MCW0114 (QTL ST53) ΠΈ ΡΠ΅ΡΡΡΠ΅Ρ
BAC-ΠΊΠ»ΠΎΠ½ΠΎΠ² Π»ΠΎΠΊΡΡΡ ADL0241 (QTL ST53). ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΠΎΠΉ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ ΠΠΠ-ΠΠΠ in situ (FISH) ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° Π²Π½ΡΡΡΠΈΡ
ΡΠΎΠΌΠΎΡΠΎΠΌΠ½Π°Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ 15-ΡΠΈ BAC-ΠΊΠ»ΠΎΠ½ΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
ΠΌΠΈΠΊΡΠΎΡΠ°ΡΠ΅Π»Π»ΠΈΡΠ½ΡΠ΅ Π»ΠΎΠΊΡΡΡ. ΠΡΡ
ΠΎΠ΄Ρ ΠΈΠ· ΡΡΠ΅Π΄Π½Π΅Π³ΠΎ ΡΠ°Π·ΠΌΠ΅ΡΠ° Π²ΡΡΠ°Π²ΠΊΠΈ β 150 Ρ.ΠΏ.Π½. β ΠΌΠΎΠΆΠ½ΠΎ Π³ΠΎΠ²ΠΎΡΠΈΡΡ ΠΎ ΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π΄Π²ΡΡ
ΡΡΠ°ΡΡΠΊΠΎΠ² Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 4 Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ ΡΡΠΌΠΌΠ°ΡΠ½ΠΎΠΉ Π΄Π»ΠΈΠ½ΠΎΠΉ 300 Ρ.ΠΏ.Π½. Π΄Π»Ρ QTL AW33 ΠΈ 1950 Ρ.ΠΏ.Π½. Π΄Π»Ρ QTL ST53
Chromosomal localization of large insert clones of the chicken genome: expanding the comparative map
Fluorescent in situ hybridization (FISH) mapping provides a convenient method for aligning clone-based physical maps with the cytogenetic map of the chicken. This also provides framework marker locations for future cytogenetic mapping using dual label techniques. In the course of screening chicken BAC libraries with linkage map markers, several BACs were selected for FISH mapping. These clones derived from filter hybridization using conventional probes for chicken genes on chromosomes 1 (MGP, MGF, TYR), 4 (ALB, ANXA5, ENDRA) and Z (CTSL, NTRK2, TPM2, and UBAP2). Cytogenetic assignments were first done for seven genes (MGP, MGF, ANXA5, ENDRA, CTSL, TPM2, and UBAP2), and chromosome location was defined more precisely for three other genes (TYR, ALB, and NTRK2). These data provide new information for comparative mapping of conserved segments of orthologous genes between chicken and human chromosomes, including GGA1-HSA12, GGA1-HSA11, GGA4-HSA4 and GGAZ-HSA9. Acknowledgements: This work was supported by grants from the Russian Foundation for Basic Researches 03-04-48060-a, Russian Ministry of Education PD02-1.4-175 and INTAS 04-2163 and by the USDA/CSREES (Project numbers: 99-35205-8566 and 2001-52100-11225)
Chromosomal localization of three GGA4 genes using BAC-based FISH mapping: a region of conserved synteny between the chicken and human genomes
The chicken homologues of three genes spaced widely on HSA4q, ANXA5 (annexin A5), ALB (albumin) and EDNRA (endothelin receptor type-A) have been mapped to the GGA4 linkage map but with some uncertainty as to their relative location (SCHMID et al. 2000; SUCHYTA et al. 2001). A cytogenetic map assignment has been made only for the ALB gene (SUZUKI et al. 1999). Here we report the chromosomal localization of all three genes on GGA4 by fluorescence in situ hybridization (FISH) using BAC clones as probes..
[Chromosomal localization of continuous genomic clones in the chicken with a view of comparative mapping] ΠΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π½Π° Ρ ΡΠΎΠΌΠΎΡΠΎΠΌΠ°Ρ ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΡΡ Π³Π΅Π½ΠΎΠΌΠ½ΡΡ ΠΊΠ»ΠΎΠ½ΠΎΠ² Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ Π² ΡΠ΅Π»ΡΡ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠ°ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ
Π€Π»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½Π°Ρ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΡ in situ (FISH) ΡΠ²Π»ΡΠ΅ΡΡΡ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΎΠ²ΠΌΠ΅ΡΠ΅Π½ΠΈΡ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠ°ΡΡ ΠΊΠΎΠ½ΡΠΈΠ³ΠΎΠ² ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΡΡ
Π³Π΅Π½ΠΎΠΌΠ½ΡΡ
ΠΊΠ»ΠΎΠ½ΠΎΠ² ΠΈ ΡΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΊΠ°ΡΡ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π΄Π²ΡΡΠ²Π΅ΡΠ½ΡΠΉ Π²Π°ΡΠΈΠ°Π½Ρ ΡΡΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΌΠΎΠΆΠ΅Ρ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ Π²Π·Π°ΠΈΠΌΠ½ΡΡ ΠΎΡΠΈΠ΅Π½ΡΠ°ΡΠΈΡ ΠΊΠ»ΠΎΠ½ΠΎΠ² Π΄ΡΡΠ³ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π΄ΡΡΠ³Π°. Π ΡΠ°ΠΌΠΊΠ°Ρ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΈ Π°Π½Π°Π»ΠΈΠ·Ρ BAC-ΠΊΠ»ΠΎΠ½ΠΎΠ², ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
ΠΈΠ·Π²Π΅ΡΡΠ½ΡΠ΅ ΠΌΠ°ΡΠΊΠ΅ΡΡ Π³ΡΡΠΏΠΏ ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΡ, Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΎ ΡΠ°ΠΊΠΈΡ
ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΡΡ
Π³Π΅Π½ΠΎΠΌΠ½ΡΡ
ΠΊΠ»ΠΎΠ½ΠΎΠ² Π±ΡΠ»ΠΈ Π²ΡΠ±ΡΠ°Π½Ρ Π΄Π»Ρ ΠΊΠ°ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ FISH. BAC-ΠΊΠ»ΠΎΠ½Ρ Π±ΡΠ»ΠΈ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Ρ ΠΏΡΡΠ΅ΠΌ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ Π½Π° ΡΠ΅ΠΏΠ»ΠΈΠΊΠ°Ρ
Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ ΠΠΠ-Π·ΠΎΠ½Π΄ΠΎΠ², ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡΠΈΡ
Π³Π΅Π½Ρ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 1 (MGP, MGF, TYR), Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 4 (ALB, ANXA5, ENDRA) ΠΈ Z-Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ (CTSL, NTRK2, TPM2 ΠΈ UBAP2). Π¦ΠΈΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ Π±ΡΠ»Π° Π²ΠΏΠ΅ΡΠ²ΡΠ΅ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π° Π΄Π»Ρ ΡΠ΅ΠΌΠΈ Π³Π΅Π½ΠΎΠ² (MGP, MGF, ANXA5, ENDRA, CTSL, TPM2 ΠΈ UBAP2). ΠΠ·Π²Π΅ΡΡΠ½Π°Ρ ΠΈΠ· ΡΠ°Π±ΠΎΡ Π΄ΡΡΠ³ΠΈΡ
Π°Π²ΡΠΎΡΠΎΠ² Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΡΡΠ΅Ρ
Π³Π΅Π½ΠΎΠ² (TYR, ALB ΠΈ NTRK2) Π±ΡΠ»Π° ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° Π±ΠΎΠ»Π΅Π΅ ΡΠΎΡΠ½ΠΎ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΡΠ³Π»ΡΠ±ΠΈΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΈΡ ΠΎΠ± ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ·ΠΌΠ΅ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈ Π΄ΠΎΠΌΠ°ΡΠ½Π΅ΠΉ ΠΊΡΡΠΈΡΡ Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ ΡΠ΅ΡΡΡΠ΅Ρ
ΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ½ΡΡ
ΠΏΠ°Ρ GGA1βHSA12, GGA1βHSA11, GGA4βHSA4 ΠΈ GGAZβHSA9.
ΠΠ°Π½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° ΡΠ°ΡΡΠΈΡΠ½ΠΎ ΡΠΈΠ½Π°Π½ΡΠΈΡΠΎΠ²Π°Π»Π°ΡΡ Π³ΡΠ°Π½ΡΠ°ΠΌΠΈ Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ Π€ΠΎΠ½Π΄Π° Π€ΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ (03-04-48060-a), ΠΠΈΠ½ΠΈΡΡΠ΅ΡΡΡΠ²Π° ΠΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π Π€ (PD02-1.4-175), INTAS (04-2163) ΠΈ USDA/CSREES (99-35205-8566 ΠΈ 2001-52100-11225).
ΠΠ΄ΡΠ΅Ρ Π΄Π»Ρ ΠΏΠ΅ΡΠ΅ΠΏΠΈΡΠΊΠΈ: Π€Π°ΠΊΡ: +7 812 428 77 33; E.mail: [email protected]
GTP-independent tRNA Delivery to the Ribosomal P-site by a Novel Eukaryotic Translation Factor*
During translation, aminoacyl-tRNAs are delivered to the ribosome by specialized GTPases called translation factors. Here, we report the tRNA binding to the P-site of 40 S ribosomes by a novel GTP-independent factor eIF2D isolated from mammalian cells. The binding of tRNAiMet occurs after the AUG codon finds its position in the P-site of 40 S ribosomes, the situation that takes place during initiation complex formation on the hepatitis C virus internal ribosome entry site or on some other specific RNAs (leaderless mRNA and A-rich mRNAs with relaxed scanning dependence). Its activity in tRNA binding with 40 S subunits does not require the presence of the aminoacyl moiety. Moreover, the factor possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40 S subunit. The corresponding gene is found in all eukaryotes and includes an SUI1 domain present also in translation initiation factor eIF1. The versatility of translation initiation strategies in eukaryotes is discussed