TRIM2, a novel member of the antiviral family, limits New World arenavirus entry

Tripartite motif (TRIM) proteins belong to a large family with many roles in host biology, including restricting virus infection. Here, we found that TRIM2, which has been implicated in cases of Charcot-Marie-Tooth disease (CMTD) in humans, acts by blocking hemorrhagic fever New World arenavirus (NWA) entry into cells. We show that Trim2-knockout mice, as well as primary fibroblasts from a CMTD patient with mutations in TRIM2, are more highly infected by the NWAs Junin and Tacaribe virus than wild-type mice or cells are. Using mice with different Trim2 gene deletions and TRIM2 mutant constructs, we demonstrate that its antiviral activity is uniquely independent of the RING domain encoding ubiquitin ligase activity. Finally, we show that one member of the TRIM2 interactome, signal regulatory protein alpha (SIRPA), a known inhibitor of phagocytosis, also restricts NWA infection and conversely that TRIM2 limits phagocytosis of apoptotic cells. In addition to demonstrating a novel antiviral mechanism for TRIM proteins, these studies suggest that the NWA entry and phagocytosis pathways overlap.Peer reviewe

Signal-regulatory protein alpha is a pan-antiviral entry factor that targets viruses using endocytic pathways

Primary and raw data from the manuscript "Signal-regulatory protein alpha is a pan-antiviral entry factor that targets viruses using endocytic pathways

TRIM2, a novel member of the antiviral family, limits new world arenavirus entry

Data from to the manuscript "TRIM2, a novel member of the antiviral family, limits new world arenavirus entry

CACNA1S haploinsufficiency confers resistance to New World arenavirus infection

Primary and raw data from the manuscript "CACNA1S haploinsufficiency confers resistance to New World arenavirus infection"THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

CACNA1S haploinsufficiency confers resistance to New World arenavirus infection

Primary and raw data from the manuscript "CACNA1S haploinsufficiency confers resistance to New World arenavirus infection"THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

TRIM2, a novel member of the antiviral family, limits new world arenavirus entry

Data related to the manuscript "TRIM2, a novel member of the antiviral family, limits new world arenavirus entry

Signal-regulatory protein alpha is a pan-antiviral entry factor that targets viruses using endocytic pathways

Primary and raw data from the manuscript "Signal-regulatory protein alpha is a pan-antiviral entry factor that targets viruses using endocytic pathways