Afiks Derivatif Pembentuk Kata Kerja dalam Bahasa Sumbawa Dialek Tongo

Munculnya judul ini dilatarbelakangi oleh adanya suatu pemikiran bahwa bahasa yang memberi kendala bagi penuturnya harus mendapat penanganan yang memadai sehingga penuturnya, makalah ini bertujuan mendeskripsikan afik-afiks pembentuk kata kerja memahami azas-azas yang ada di dalam bahasanya. Dengan adanya pemahaman yang memadai terhadap azas-azas yang ada dalam bahasanya, penutur bahasa yang bersangkutan tidak akan tejebak dalam carut-marut berbahasa dan carut-marut berpikir menggunakan bahasanya. Dengan demikiandan azas atau kaidah pembentukan kata kerja dalam bahasa bahasa Sumbawa dialek Tongo. Untuk menjawab permasalahan tersebut, peneliti menggunakan metode distribusional dengan teknik urai unsur langsung, teknik sisip, teknik ganti, teknik oposisi biner dan metode padan dengan teknik alat penentu referen dan alat penentu bahasa lain. Melalui penerapan kedua metode tersebut, diketahuilah bahwa terdapat tujuh afiks derivatif, yakni: {ba-}, {ra-}{ i-}, { sa-}1, {sa-}, {N-}, { ka1-}, dan {ka2-}

Spectral Difference Equations Satisfied by KP Soliton Wavefunctions

The Baker-Akhiezer (wave) functions corresponding to soliton solutions of the KP hierarchy are shown to satisfy eigenvalue equations for a commutative ring of translational operators in the spectral parameter. In the rational limit, these translational operators converge to the differential operators in the spectral parameter previously discussed as part of the theory of "bispectrality". Consequently, these translational operators can be seen as demonstrating a form of bispectrality for the non-rational solitons as well.Comment: to appear in "Inverse Problems

FORMULASI SUBSTRAT DASAR KOTORAN AYAM DAN LIMBAH CAIR TEMPE DENGAN INOKULUM RUMEN SAPI UNTUK STUDI AWAL PRODUKSI BIOGAS

Production of biogas using the basic substrate of tempeh’s liquid waste and chicken’s waste with the bacteria inoculum of cow’s rumen has been experimentally conducted. This study aims to produce biogas using the substrate with the bacteria inoculum of cow’s rumen and know the optimal mixture ratio of the basic substrate and the inoculum. In this study, the amounts of basic substrate applied were 500 g chicken’s waste and 500 mL liquid waste of tempeh. The study was designed using Complete Random Design comprising 6 treatments with 3 times repetitions, respectively. The treatments tested were P0 (100% basic substrate), P1 (80% basic substrate + 20% inoculum), P2 (60% basic substrate + 40% inoculum), P3 (40% basic substrate + 60% inoculum), P4 (20% basic substrate + 80% inoculum), P5 (100% inoculum). These treatments were fermented with anaerob bacteria for 10 days in the fermentor 1000 mL mounted with the biogas collecting balloon. Variables observed in this study were the measurements of biogas volume, culture medium pH, and biogas burning temperature, and the testing of biogas flame colour. As result of observation, unlike P5, the examined treatments of P0, P1, P2, P3 and P4 could able to produce biogas with the respectively produced volume of 53,6 cm3, 56,7 cm3, 30,0 cm3, 90,0 cm3 and 73,3 cm3. While, the measured values of culture medium pH and biogas burning temperature were 6,23-6,63 and 152oC with the flame of red colour. This study shows that the optimal mixture ratio of the basic substrate and the inoculum for producing biogas is 2:3, and the produced biogas is potential to be used as an alternative energy. Keywords: Biogas, Chicken’s waste, Liquid waste of tempeh, Cow’s rume

T4-Related Bacteriophage LIMEstone Isolates for the Control of Soft Rot on Potato Caused by ‘Dickeya solani’

The bacterium ‘Dickeya solani’, an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_LIMEstone2. The LIMEstone phages have a T4-related genome organization and share DNA similarity with Salmonella phage ViI. Microbiological and molecular characterization of the phages deemed them suitable and promising for use in phage therapy. The phages reduced disease incidence and severity on potato tubers in laboratory assays. In addition, in a field trial of potato tubers, when infected with ‘Dickeya solani’, the experimental phage treatment resulted in a higher yield. These results form the basis for the development of a bacteriophage-based biocontrol of potato plants and tubers as an alternative for the use of antibiotics

Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis)

<p>Abstract</p> <p>Background</p> <p>The cotton boll weevil (<it>Anthonomus grandis</it>) is a serious insect-pest in the Americas, particularly in Brazil. The use of chemical or biological insect control is not effective against the cotton boll weevil because of its endophytic life style. Therefore, the use of biotechnological tools to produce insect-resistant transgenic plants represents an important strategy to reduce the damage to cotton plants caused by the boll weevil. The present study focuses on the identification of novel molecules that show improved toxicity against the cotton boll weevil. <it>In vitro </it>directed molecular evolution through DNA shuffling and phage display screening was applied to enhance the insecticidal activity of variants of the Cry8Ka1 protein of <it>Bacillus thuringiensis</it>.</p> <p>Results</p> <p>Bioassays carried out with <it>A. grandis </it>larvae revealed that the LC₅₀of the screened mutant Cry8Ka5 toxin was 3.15-fold higher than the wild-type Cry8Ka1 toxin. Homology modelling of Cry8Ka1 and the Cry8Ka5 mutant suggested that both proteins retained the typical three-domain Cry family structure. The mutated residues were located mostly in loops and appeared unlikely to interfere with molecular stability.</p> <p>Conclusions</p> <p>The improved toxicity of the Cry8Ka5 mutant obtained in this study will allow the generation of a transgenic cotton event with improved potential to control <it>A. grandis</it>.</p

Global, regional, and national burden of epilepsy, 1990 - 2016 : a systematic analysis for the Global Burden of Disease Study 2016

Background: Seizures and their consequences contribute to the burden of epilepsy because they can cause health loss (premature mortality and residual disability). Data on the burden of epilepsy are needed for health-care planning and resource allocation. The aim of this study was to quantify health loss due to epilepsy by age, sex, year, and location using data from the Global Burden of Diseases, Injuries, and Risk Factors Study. Methods: We assessed the burden of epilepsy in 195 countries and territories from 1990 to 2016. Burden was measured as deaths, prevalence, and disability-adjusted life-years (DALYs; a summary measure of health loss defined by the sum of years of life lost [YLLs] for premature mortality and years lived with disability), by age, sex, year, location, and Socio-demographic Index (SDI; a compound measure of income per capita, education, and fertility). Vital registrations and verbal autopsies provided information about deaths, and data on the prevalence and severity of epilepsy largely came from population representative surveys. All estimates were calculated with 95% uncertainty intervals (UIs). Interpretation: Despite the decrease in the disease burden from 1990 to 2016, epilepsy is still an important cause of disability and mortality. Standardised collection of data on epilepsy in population representative surveys will strengthen the estimates, particularly in countries for which we currently have no or sparse data and if additional data is collected on severity, causes, and treatment. Sizeable gains in reducing the burden of epilepsy might be expected from improved access to existing treatments in low-income countries and from the development of new effective drugs worldwide