Gap filling and noise reduction of unevenly sampled data by means of the Lomb-Scargle periodogram

International audienceThe Lomb-Scargle periodogram is widely used for the estimation of the power spectral density of unevenly sampled data. A small extension of the algorithm of the Lomb-Scargle periodogram permits the estimation of the phases of the spectral components. The amplitude and phase information is sufficient for the construction of a complex Fourier spectrum. The inverse Fourier transform can be applied to this Fourier spectrum and provides an evenly sampled series (Scargle, 1989). We are testing the proposed reconstruction method by means of artificial time series and real observations of mesospheric ozone, having data gaps and noise. For data gap filling and noise reduction, it is necessary to modify the Fourier spectrum before the inverse Fourier transform is done. The modification can be easily performed by selection of the relevant spectral components which are above a given confidence limit or within a certain frequency range. Examples with time series of lower mesospheric ozone show that the reconstruction method can reproduce steep ozone gradients around sunrise and sunset and superposed planetary wave-like oscillations observed by a ground-based microwave radiometer at Payerne

Long-term observation of midlatitude quasi 2-day waves by a water vapor radiometer

A mesospheric water vapor data set obtained by the middle atmospheric water vapor radiometer (MIAWARA) close to Bern, Switzerland (46.88°&thinsp;N, 7.46°&thinsp;E) during October 2010 to September 2017 is investigated to study the long-term evolution and variability of quasi 2-day waves (Q2DWs). We present a climatological overview and an insight on the dynamical behavior of these waves with the occurring spectrum of periods as seen from a midlatitude observation site. Such a large and nearly continuous measurement data set as ours is rare and of high scientific value. The core results of our investigation indicate that the activity of the Q2DW manifests in burst-like events and is higher during winter months (November–February) than during summer months (May–August) for the altitude region of the mesosphere (up to 0.02&thinsp;hPa in winter and up to 0.05&thinsp;hPa in summer) accessible for the instrument. Single Q2DW events reach at most about 0.8&thinsp;ppm in the H2O amplitudes. Further, monthly mean Q2DW amplitude spectra are presented and reveal a high-frequency variability between different months. A large fraction of identified Q2DW events (20&thinsp;%) develop periods between 38 and 40&thinsp;h. Further, we show the temporal evolution of monthly mean Q2DW oscillations continuously for all months and separated for single months over 7 years. The analysis of autobicoherence spectra gives evidence that Q2DWs are sometimes phase coupled to diurnal oscillations to a high degree and to waves with a period close to 18&thinsp;h.</p

Global sounding of F region irregularities by COSMIC during a geomagnetic storm

We analyse reprocessed electron density profiles and total electron content (TEC) profiles of the ionosphere in September 2008 (around solar minimum) and September 2013 (around solar maximum) obtained by the Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC/FORMOSAT-3). The TEC profiles describe the total electron content along the ray path from the GPS satellite to the low Earth orbit as function of the tangent point of the ray. Some of the profiles in the magnetic polar regions show small-scale fluctuations on spatial scales &lt;50&thinsp;km. Possibly the trajectory of the tangent point intersects spatial electron density irregularities in the magnetic polar region. For derivation of the morphology of the electron density and TEC fluctuations, a 50&thinsp;km high-pass filter is applied in the s domain, where s is the distance between a reference point (bottom tangent point) and the tangent point. For each profile, the mean of the fluctuations is calculated for tangent point altitudes between 400 and 500&thinsp;km. At first glance, the global maps of ΔNe and ΔTEC are quite similar. However, ΔTEC might be more reliable since it is based on fewer retrieval assumptions. We find a significant difference if the arithmetic mean or the median is applied to the global map of September 2013. In agreement with literature, ΔTEC is enhanced during the post-sunset rise of the equatorial ionosphere in September 2013, which is associated with spread F and equatorial plasma bubbles. The global map of ΔTEC at solar maximum (September 2013) has stronger fluctuations than those at solar minimum (September 2008). We obtained new results when we compare the global maps of the quiet phase and the storm phase of the geomagnetic storm of 15 July 2012. It is evident that the TEC fluctuations are increased and extended over the southern magnetic polar region at the day of the geomagnetic storm. The north–south asymmetry of the storm response is more pronounced in the upper ionosphere (ray tangent points h&thinsp;=&thinsp;400–500&thinsp;km) than in the lower ionosphere (ray tangent points h&thinsp;=&thinsp;200–300&thinsp;km).</p

Trend analysis of the 20-year time series of stratospheric ozone profiles observed by the GROMOS microwave radiometer at Bern

The ozone radiometer GROMOS (GROund-based Millimeter-wave Ozone Spectrometer) has been performing continuous observations of stratospheric ozone profiles since 1994 above Bern, Switzerland (46.95° N, 7.44° E, 577 m). GROMOS is part of the Network for the Detection of Atmospheric Composition Change (NDACC). From November 1994 to October 2011, the ozone line spectra were measured by a filter bench (FB). In July 2009, a fast Fourier transform spectrometer (FFTS) was added as a back end to GROMOS. The new FFTS and the original FB measured in parallel for over 2 years. The ozone profiles retrieved separately from the ozone line spectra of FB and FFTS agree within 5% at pressure levels from 30 to 0.5 hPa, from October 2009 to August 2011. A careful harmonisation of both time series has been carried out by taking the FFTS as the reference instrument for the FB. This enables us to assess the long-term trend derived from stratospheric ozone observations at Bern. The trend analysis was performed by using a robust multilinear parametric trend model which includes a linear term, the solar variability, the El Niño–Southern Oscillation (ENSO) index, the quasi-biennial oscillation (QBO), the annual and semi-annual oscillation and several harmonics with period lengths between 3 and 24 months. Over the last years, some experimental and modelling trend studies have shown that the stratospheric ozone trend is levelling off or even turning positive. With our observed ozone profiles, we are able to support this statement by reporting a statistically significant trend of +3.14% decade$^{-1}$ at 4.36 hPa (37.76 km), covering the period from January 1997 to January 2015, above Bern. Additionally, we have estimated a negative trend over this period of -3.94%decade$^{-1}$ at 0.2 hPa (59 km)

Overexpression of heat shock protein 27 (HSP27) increases gemcitabine sensitivity in pancreatic cancer cells through S-phase arrest and apoptosis

We previously established a role for HSP27 as a predictive marker for therapeutic response towards gemcitabine in pancreatic cancer. Here, we investigate the underlying mechanisms of HSP27-mediated gemcitabine sensitivity. Utilizing a pancreatic cancer cell model with stable HSP27 overexpression, cell cycle arrest and apoptosis induction were analysed by flow cytometry, nuclear staining, immunoblotting and mitochondrial staining. Drug sensitivity studies were performed by proliferation assays. Hyperthermia was simulated using mild heat shock at 41.8 degrees C. Upon gemcitabine treatment, HSP27-overexpressing cells displayed an early S-phase arrest subsequently followed by a strongly increased sub-G1 fraction. Apoptosis was characterized by PARP-, CASPASE 3-, CASPASE 8-, CASPASE 9- and BIM- activation along with a mitochondrial membrane potential loss. It was reversible through chemical caspase inhibition. Importantly, gemcitabine sensitivity and PARP cleavage were also elicited by heat shock-induced HSP27 overexpression, although to a smaller extent, in a panel of pancreatic cancer cell lines. Finally, HSP27-overexpressing pancreatic cancer cells displayed an increased sensitivity also towards death receptor-targeting agents, suggesting another pro-apoptotic role of HSP27 along the extrinsic apoptosis pathway. Taken together, in contrast to the well-established anti-apoptotic properties of HSP27 in cancer, our study reveals novel pro-apoptotic functions of HSP27mediated through both the intrinsic and the extrinsic apoptotic pathwaysat least in pancreatic cancer cells. HSP27 could represent a predictive marker of therapeutic response towards specific drug classes in pancreatic cancer and provides a novel molecular rationale for current clinical trials applying the combination of gemcitabine with regional hyperthermia in pancreatic cancer patients

Significant decline of mesospheric water vapor at the NDACC site near Bern in the period 2007 to 2018

The middle atmospheric water vapor radiometer MIAWARA is located close to Bern in Zimmerwald (46.88∘&thinsp;N, 7.46∘&thinsp;E; 907 m) and is part of the Network for the Detection of Atmospheric Composition Change (NDACC). Initially built in the year 2002, a major upgrade of the instrument's spectrometer allowed middle atmospheric water vapor to be continuously measured since April 2007. Thenceforward to May 2018, a time series of more than 11 years has been gathered, that makes a first trend estimate possible. For the trend estimation, a robust multilinear parametric trend model has been used. The trend model encompasses a linear term, a solar activity tracker, the El Niño–Southern Oscillation (ENSO) index and the Quasi-Biennial Oscillation (QBO) as well as the annual and semi-annual oscillation. In the time period April 2007 to May 2018 we find a significant decline in water vapor by -0.6±0.2&thinsp;ppm per decade between 61 and 72 km. Below the stratopause level (∼48 km) a smaller reduction of H2O of up to -0.3±0.1&thinsp;ppm per decade is detected.</p

Origin of solvent-induced polymorphism in self-assembly of trimesic acid monolayers at solid-liquid interfaces

Encoding information in the chemical structure of tectons is the pivotal strategy in self-assembly for the realization of targeted supramolecular structures. However, frequently observed polymorphism in supramolecular monolayers provides experimental evidence for a decisive additional influence of environmental parameters, such as solute concentration or type of solvent, on structure selection. While concentration-induced polymorphism is comparatively well understood, the thermodynamical and molecular origins of solvent-induced polymorphism remain elusive. To shed light on this fundamental aspect of self-assembly, we explore the solvent-induced polymorphism of trimesic acid (TMA) monolayers on graphite as prototypical example. Using the homologous series of fatty acids as solvents, TMA self-assembles into the anticipated chickenwire polymorph for longer chain fatty acids, whereas the more densely packed, but still porous flower polymorph emerges in shorter chain fatty acids. According to our initial working hypothesis, the origin of this solvent-induced polymorphism lies in a solvent-dependence of the free energy gain. Utilizing an adapted Born-Haber cycle constructed from measured TMA sublimation and dissolution enthalpies as well as Density Functional Theory calculated monolayer binding energies, we quantitatively assessed the self-assembly thermodynamics of both polymorphs in hexanoic, heptanoic, and nonanoic acid. Yet, in contrast to the experimental findings, these results suggest superior thermodynamical stability of the chickenwire polymorph in all solvents. On the other hand, additional experiments comprising variable temperature Scanning Tunneling Microscopy corroborate that the flower polymorph is thermodynamically most stable in hexanoic acid. To resolve this apparent contradiction, we propose a thermodynamical stabilization of the flower polymorph in hexanoic acid through the stereochemically specific co-adsorption of shape-matched solvent molecules in its unique smaller elongated pores. This alternative explanation gains further support from experiments with side-substituted hexanoic acid solvents. Combination of a quantitative thermodynamic analysis and studies with systematic variations of the solvent’s molecular structure holds great promise to enhance the understanding of thus far underexplored solvent effects

Comparison and synergy of stratospheric ozone measurements by satellite limb sounders and the ground-based microwave radiometer SOMORA

International audienceStratospheric O3 profiles obtained by the satellite limb sounders Aura/MLS, ENVISAT/MIPAS, ENVISAT/GOMOS, SAGE-II, SAGE-III, UARS/HALOE are compared to coincident O3 profiles of the ground-based microwave radiometer SOMORA in Switzerland. Data from the various measurement techniques are within 10% at altitudes below 45 km. At altitudes 45?60 km, the relative O3 differences are within a range of 50% Larger deviations at upper altitudes are attributed to larger relative measurement errors caused by lower O3 concentrations. The spatiotemporal characteristics of the O3 differences (satellite ? ground station) are investigated by analyzing about 5000 coincident profile pairs of Aura/MLS (retrieval version 1.5) and SOMORA. The probability density function of the O3 differences is represented by a Gaussian normal distribution (except for profile pairs around the stratopause at noon). The dependence of the O3 differences on the horizontal distance between the sounding volumes of Aura/MLS and SOMORA is derived. While the mean bias (Aura/MLS ? SOMORA) is constant with increasing horizontal distance (up to 800 km), the standard deviation of the O3 differences increases from around 8 to 12% in the mid-stratosphere. Geographical maps yield azimuthal dependences and horizontal gradients of the O3 difference field around the SOMORA ground station. Coherent oscillations of O3 are present in the time series of Aura/MLS and SOMORA (e.g., due to traveling planetary waves). Ground- and space-based measurements often complement one another. We introduce the double differencing technique which allows both the cross-validation of two satellites by means of a ground station and the cross-validation of distant ground stations by means of one satellite. Temporal atmospheric noise in the geographical ozone map over Payerne is significantly reduced by combination of the data from SOMORA and Aura/MLS. These analyses illustrate the synergy between ground-based and space-based measurements

Optimization of cell-laden bioinks for 3D bioprinting and efficient infection with influenza A virus

Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of alginate, gelatin and Matrigel were used to provide a scaffold for a 3D arrangement of human alveolar A549 cells. A blend containing 20% Matrigel provided the optimal conditions for spatial distribution and viability of the printed cells. Infection of the 3D model with a seasonal influenza A strain resulted in widespread distribution of the virus and a clustered infection pattern that is also observed in the natural lung but not in two-dimensional (2D) cell culture, which demonstrates the advantage of 3D printed constructs over conventional culture conditions. The bioink supported viral replication and proinflammatory interferon release of the infected cells. We consider our strategy to be paradigmatic for the generation of humanized 3D tissue models by bioprinting to study infections and develop new antiviral strategies.DFG, 325093850, Open Access Publizieren 2017 - 2018 / Technische Universität Berli