MIP-TFMAA-co-TRIM as Selective Adsorption of β-sitosterol

A functional monomer of trifluoromethylacrylic acid (TFMAA), a cross-linker of trimethylolpropane trimethacrylate (TRIM) and a template molecule of β-sitosterol have been used to produce a molecularly imprinted polymer, MIP_TFMAA-co-TRIM by a polymerization process based on non-covalent interaction between the monomer and the template. After the removal of the template, we used the product of MIP as an adsorbent of β-sitosterol and determined the adsorption capacity. The adsorption-desorption process for β-sitosterol and the selectivity test for β-sitosterol and cholesterol were also studied. High Performance Liquid Chromatography (HPLC) and UV-vis spectrophotometry were methods for measuring the amount of .materials adsorbed. Langmuir and Freundlich equations were models used to study the adsorption isotherm of the removal of β-sitosterol. The adsorption of β-sitosterol on MIP_TFMAA-co-TRIM followed the Freundlich isotherm with a capacity of 0.61 mg/g. The first and the second adsorption-desorption processes showed that the recovery percentages of β-sitosterol from MIP_TFMAA-co-TRIM were 66.36 and 40.56%, respectively. The MIP was more selective for β-sitosterol than for cholesterol

Aktivitas Antibakteri Dari Fraksi Artocarpus Integer (Thunb.) Merr. Dengan Metode Difusi Agar (Antibacterial Activity of Artocarpus Integer (Thunb.) Merr. Fraction by Difusi Agar Method).

Artocarpus integer (Thunb.) Merr. (Cempedak) merupakan tanaman famili Moraceae yang banyak dibudidayakan di Luwu, Sulawesi Selatan. Ekstraksi dan uji bioaktivitas antibakteri kayu batang Artocarpus integer (Thunb.) Merr. telah dilakukan. Kayu batang diserut, dikeringkan, dihaluskan, dan dimaserasi dengan metanol hingga diperoleh ekstrak kasar metanol. Ekstrak metanol kemudian dipartisi berturut-turut dalam n-heksan, kloroform, dan etil asetat sehingga diperoleh fraksi n-heksan, kloroform, etil asetat, dan metanol. Uji aktivitas antibakteri dengan metode difusi agar menggunakan 2 bakteri gram negatif (E. coli dan S. typhi) dan 2 gram positif (S. aureus dan S. pneumonia). Zona hambat terbesar yang diperoleh masing-masing fraksi adalah fraksi n-heksan sebesar 12,80 mm pada bakteri S. pneumonia, fraksi kloroform sebesar 13,80 mm pada bakteri S. pneumonia, fraksi etil asetat sebesar 9,80 mm pada bakteri S. typhi, dan fraksi metanol sebesar 12,90 pada bakteri S. pneumonia. Fraksi kloroform berpotensi kuat sebagai antibakteri karena memiliki daya hambat terbesar terhadap S. pneumonia