Cloud 9: Bringing the abstract to light

This document chronicles my journey designing the lighting for Carol Churchill’s Cloud 9 in the Creative Arts Center at West Virginia University. Starting with the initial read, this paper will continue through the early meetings and initial concepts discussed by the design team. Proceeding through the evolution of the design up to and throughout the tech process and will conclude with an evaluation of the overall experience. I have also provided a combination of paperwork items which were essential in the mounting of the production including: light plot, the channel hookup, cue list, and the EOS magic sheet. In addition, there are images from initial research and from the production itself

A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome

Multinucleate cellular syncytial formation is a hallmark of skeletal muscle differentiation. Myomaker, encoded by Mymk (Tmem8c), is a well-conserved plasma membrane protein required for myoblast fusion to form multinucleated myotubes in mouse, chick, and zebrafish. Here, we report that autosomal recessive mutations in MYMK (OMIM 615345) cause Carey-Fineman-Ziter syndrome in humans (CFZS; OMIM 254940) by reducing but not eliminating MYMK function. We characterize MYMK-CFZS as a congenital myopathy with marked facial weakness and additional clinical and pathologic features that distinguish it from other congenital neuromuscular syndromes. We show that a heterologous cell fusion assay in vitro and allelic complementation experiments in mymk knockdown and mymkinsT/insT zebrafish in vivocan differentiate between MYMK wild type, hypomorphic and null alleles. Collectively, these data establish that MYMK activity is necessary for normal muscle development and maintenance in humans, and expand the spectrum of congenital myopathies to include cell-cell fusion deficits

Probing the Inner Circumgalactic Medium and Quasar Illumination around the Reddest `Extremely Red Quasar' (ERQ)

Dusty quasars might be in a young stage of galaxy evolution with prominent quasar feedback. A recently discovered population of luminous, extremely red quasars at $z\sim$~2--4 has extreme spectral properties related to exceptionally powerful quasar-driven outflows. We present Keck/KCWI observations of the reddest known ERQ, at $z=$\,2.3184, with extremely fast [\ion{O}{III}]~$\lambda$5007 outflow at $\sim$6000~km~s$^{-1}$. The Ly$\alpha$ halo spans $\sim$100~kpc. The halo is kinematically quiet, with velocity dispersion $\sim$300~km~s$^{-1}$ and no broadening above the dark matter circular velocity down to the spatial resolution $\sim$6~kpc from the quasar. We detect spatially-resolved \ion{He}{II}~$\lambda$1640 and \ion{C}{IV}~$\lambda$1549 emissions with kinematics similar to the Ly$\alpha$ halo and a narrow component in the [\ion{O}{III}]~$\lambda$5007. Quasar reddening acts as a coronagraph allowing views of the innermost halo. A narrow Ly$\alpha$ spike in the quasar spectrum is inner halo emission, confirming the broad \ion{C}{IV}~$\lambda$1549 in the unresolved quasar is blueshifted by $2240$~km~s$^{-1}$ relative to the halo frame. We propose the inner halo is dominated by moderate-speed outflow driven in the past and the outer halo dominated by inflow. The high central concentration of the halo and the symmetric morphology of the inner region are consistent with the ERQ being in earlier evolutionary stage than blue quasars. The \ion{He}{II}~$\lambda$1640/Ly$\alpha$ ratio of the inner halo and the asymmetry level of the overall halo are dissimilar to Type~II quasars, suggesting unique physical conditions for this ERQ that are beyond orientation differences from other quasar populations. We find no evidence of mechanical quasar feedback in the Ly$\alpha$-emitting halo.Comment: 20 pages, 18 figures, published in MNRA

Introduction to special section: Outstanding problems in quantifying the radiative impact of mineral dust

International audienceThis paper provides an introduction to the special section of the Journal of Geophysical Research on mineral dust. We briefly review the current experimental and theoretical approaches used to quantify the dust radiative impacts, highlight the outstanding issues, and discuss possible strategies to overcome the emerging problems. We also introduce the contributing papers of this special section. Despite the recent notable advances in dust studies, we demonstrate that the radiative effects of dust remain poorly quantified due to both limited data and incomplete understanding of relative physical and chemical processes. The foremost needs are (1) to quantify the spatial and temporal variations of dust burden in the atmosphere and develop a predictive capability for the size‐ and composition‐resolved dust particle distribution; (2) to develop a quantitative description of the processes that control the spatial and temporal variabilities of dust physical and chemical properties and radiative effects; (3) to develop new instrumentation (especially to measure the dust particle size distribution in a wide range from about 0.01 μm to 100 μm, scattering phase function and light absorption by dust particles); and (4) to develop new techniques for interpreting and merging the diverse information from satellite remote sensing, in situ and ground‐based measurements, laboratory studies, and model simulations. Because dust distribution and effects are heterogeneous, both spatially and temporally, a promising strategy to advance our knowledge is to perform comprehensive studies at the targeted regions affected by mineral dust of both natural and anthropogenic origin

Thermal Shift Assay for Small GTPase Stability Screening: Evaluation and Suitability

Thermal unfolding methods are commonly used as a predictive technique by tracking the protein's physical properties. Inherent protein thermal stability and unfolding profiles of biotherapeutics can help to screen or study potential drugs and to find stabilizing or destabilizing conditions. Differential scanning calorimetry (DSC) is a 'Gold Standard' for thermal stability assays (TSA), but there are also a multitude of other methodologies, such as differential scanning fluorimetry (DSF). The use of an external probe increases the assay throughput, making it more suitable for screening studies, but the current methodologies suffer from relatively low sensitivity. While DSF is an effective tool for screening, interpretation and comparison of the results is often complicated. To overcome these challenges, we compared three thermal stability probes in small GTPase stability studies: SYPRO Orange, 8-anilino-1-naphthalenesulfonic acid (ANS), and the Protein-Probe. We studied mainly KRAS, as a proof of principle to obtain biochemical knowledge through TSA profiles. We showed that the Protein-Probe can work at lower concentration than the other dyes, and its sensitivity enables effective studies with non-covalent and covalent drugs at the nanomolar level. Using examples, we describe the parameters, which must be taken into account when characterizing the effect of drug candidates, of both small molecules and Designed Ankyrin Repeat Proteins