Steady state electrospun polyamide nanofibres for the use in MBR

Electrospinning is the process to generate nanofibers. Some very specific application fields are targeted in the literature about electrospinning. One of these research fields is filtration. In this field, mainly air filtration is targeted. Recent studies showed the strong performance of nanofibrous material in liquid filtration. For liquid (such as water) filtration the material needs to be flawless and pressure resistant. To cope with the first problem, we have developed a specific technology based on nozzle electrospinning. The key feature in our technology is the possibility to electrospin under steady state conditions. In each time period, the amount of polymer that is pumped out of the needle as a solution is the same as the amount of polymer that is deposited in the form of nanofibers. In the case of water filtration, a polyamide 6 nanofibrous nonwoven is created. The polyamide is chosen because of its high inherent strength. This high mechanical strength makes it possible for the material to withstand the pressure applied on the material during filtration. At this moment, projects are running to investigate if the polyamide 6 nanofibrous structures give an added value in the field of water filtration. The first results show that nanofibres enhance the water flux values at the same pressure. Next to flux investigations, experiments concerning the use of the membranes in a membrane bioreactor (MBR) are performed. These experiments show that the nanofibrous material has the same filtration efficiency as commercial MBR membranes. It even surpasses that efficiency with an extra functionalisation

Functionalized nanofiber membranes for disinfection of water

Nowadays, water is often disinfected with biocides which can result in the formation of harmful by-products. Therefore new developments in water treatment are needed. One of such new developments is presented in this study. Nanofiber membranes produced by the electrospinning technique offer great potential in water filtration. Nanofiber structures have a small pore size, a large specific surface area and interconnected open pore structure which makes them appropriate for water filtration. However, the pathogen removal efficiency is not yet satisfactory. To improve this, disinfectants as a functional agent on nanofiber membranes were used in this study. The pathogen removal of the functionalized membranes was evaluated with wastewater from a general hospital with ca. 107 culturable organisms per 100 ml before filtration. Pathogen removal of about 4 to 5,5 log10 is possible with selected biocides. This is higher than a nanofibers membrane without disinfectant, which gives a removal of 2 log10 and commercial membranes with a removal of 3 to 4 log10. In addition to this, the biocides stay immobilized inside the membrane and only a limited fraction (10%) leaches into the water

A retrospective study on semen quality parameters from four different Dutch horse breeds with different levels of inbreeding

A high degree of inbreeding has been reported to negatively impact semen quality in Friesian horses and Shetland ponies. Both breeds are characterized by a closed studbook, small population size, and high incidence of inbreeding. The Dutch Warmblood studbook (KWPN: Koninklijk Warmblood Paardenstamboek Nederland) is a much larger studbook with two distinct populations: the KWPN-Riding horses, managed as an 'open' studbook, and the KWPN-Harness horses, representing a much smaller subpopulation within the KWPN breed and managed as an 'almost closed' studbook. It was recently reported that the degree of inbreeding in KWPN-Harness horses has increased in recent decades due to the small gene pool; however, the degree of inbreeding is still lower than that of Friesian horses and Shetland ponies. We hypothesized that a high or rising degree of inbreeding might negatively impact semen quality. In the present study, we retrospectively compared semen quality parameters of stallions from four different breeds or types (Friesian Horses, Shetland Ponies, KWPN-Riding horses, and KWPN-Harness horses), each reported with different degrees of inbreeding. Semen concentration, and percentages of motile, morphologically normal and live spermatozoa, and the total number of morphologically normal, progressive motile spermatozoa per ejaculate (TNM) were analyzed for 2832 semen evaluations performed over a 15-year period. KWPN-Harness horses had a significantly lower sperm concentration, % motile spermatozoa and % live spermatozoa than KWPN-Riding horses but the % motile and % morphologically normal spermatozoa and TNM in both KWPN-Harness and KWPN-Riding horses were significantly higher than in Friesian horses and Shetland ponies. These results suggest a lower semen quality in KWPN-Harness than KWPN-Riding horses, potentially as a result of a higher coefficient of inbreeding. The negative trend observed in the KWPN-Harness horses may be a warning sign, and breeders or stud books should monitor the degree of inbreeding carefully to avoid a further reduction in semen quality, to the levels observed in Friesian horses and Shetland ponies

Pure preovulatory follicular fluid promotes in vitro maturation of in vivo aspirated equine oocytes

International audienceIn the mare, rates of fertilization and development are low in oocytes matured in vitro, and a closer imitation of in vivo conditions during oocyte maturation might be beneficial. The aims of the present study were, therefore, to investigate whether (1) equine oocytes can be matured in vitro in pure equine preovulatory follicular fluid, (2) priming of the follicular fluid donor with crude equine gonadotrophins (CEG) before aspiration of preovulatory follicular fluid promotes the in vitro maturation rate, (3) the in vitro maturation rate differs between oocytes aspirated during estrus and those aspirated again 8 days after the initial follicular aspiration, and (4) high follicular concentrations of meiosis activating sterols (MAS) are beneficial for in vitro maturation of equine oocytes. During estrus, 19 pony mares were treated with 25 mg CEG. After 24 h (A1) and again after 8 days (A2), all follicles ≥4 mm were aspirated and incubated individually for 30 h in the following culture media: standard culture medium (SM), preovulatory follicular fluid collected before CEG containing low MAS concentrations (FF1), preovulatory follicular fluid collected before CEG containing high MAS concentrations (FF2) or preovulatory follicular fluid collected 35 h after administration of CEG containing low MAS concentrations (FF3). Cumulus expansion rate was significantly affected by culture medium. The overall nuclear maturation rate was significantly higher for oocytes collected at A1 (67%) than for oocytes collected at A2 (30%). For oocytes collected at A1, the maturation rates were 71% (FF1), 61% (FF2), 79% (FF3) and 56% (SM). An electrophoretic protein analysis of the culture media revealed the presence of a 200-kDa protein in FF3. The results demonstrate that (1) equine oocytes can be matured during culture in pure equine preovulatory follicular fluid, (2) preovulatory follicular fluid collected after gonadotrophin-priming seems superior in supporting in vitro maturation than standard culture medium, (3) oocytes aspirated 8 days after a previous aspiration are less competent for in vitro maturation than oocytes recovered during the initial aspiration, and (4) the regulation of meiotic resumption during in vitro culture of equine oocytes might be related to the presence of a 200-kDa protein

A retrospective study on semen quality parameters from four different Dutch horse breeds with different levels of inbreeding

A high degree of inbreeding has been reported to negatively impact semen quality in Friesian horses and Shetland ponies. Both breeds are characterized by a closed studbook, small population size, and high incidence of inbreeding. The Dutch Warmblood studbook (KWPN: Koninklijk Warmblood Paardenstamboek Nederland) is a much larger studbook with two distinct populations: the KWPN-Riding horses, managed as an 'open' studbook, and the KWPN-Harness horses, representing a much smaller subpopulation within the KWPN breed and managed as an 'almost closed' studbook. It was recently reported that the degree of inbreeding in KWPN-Harness horses has increased in recent decades due to the small gene pool; however, the degree of inbreeding is still lower than that of Friesian horses and Shetland ponies. We hypothesized that a high or rising degree of inbreeding might negatively impact semen quality. In the present study, we retrospectively compared semen quality parameters of stallions from four different breeds or types (Friesian Horses, Shetland Ponies, KWPN-Riding horses, and KWPN-Harness horses), each reported with different degrees of inbreeding. Semen concentration, and percentages of motile, morphologically normal and live spermatozoa, and the total number of morphologically normal, progressive motile spermatozoa per ejaculate (TNM) were analyzed for 2832 semen evaluations performed over a 15-year period. KWPN-Harness horses had a significantly lower sperm concentration, % motile spermatozoa and % live spermatozoa than KWPN-Riding horses but the % motile and % morphologically normal spermatozoa and TNM in both KWPN-Harness and KWPN-Riding horses were significantly higher than in Friesian horses and Shetland ponies. These results suggest a lower semen quality in KWPN-Harness than KWPN-Riding horses, potentially as a result of a higher coefficient of inbreeding. The negative trend observed in the KWPN-Harness horses may be a warning sign, and breeders or stud books should monitor the degree of inbreeding carefully to avoid a further reduction in semen quality, to the levels observed in Friesian horses and Shetland ponies

Electrospun nanofibre membranes functionalised with TiO2 nanoparticles: Evaluation of humic acid and bacterial removal from polluted water

This study presents contact experiments and filtration tests with nanofibre membranes containing TiO2 nanoparticles in view of effluent treatment. The first part of this study focuses on the removal of dissolved organic matter, more specifically humic acids. Removal of humic acids from secondary wastewater treatment plant effluent as well as from synthetic water solutions was tested. Also the bactericidal effect of the TiO2 functionalised nanofibre membranes was examined. Contact experiments with TiO2 functionalised membranes showed removal of humic acids (83% degradation after 2 h) and Staphylococcus aureus (4.5 log(10)/100 ml after 6 h). Also the possibility of using (TiO2 functionalised) nanofibre membranes for effluent filtration in view of water reuse, was examined. Such secondary effluent filtration tests improved water quality as a reduction in turbidity (69%), humic acids (37%) and bacterial activity (76%) was observed. It can be concluded that nanofibre membranes could be used as a high-flux filtration technique for effluent recuperation

A retrospective study on semen quality parameters from four different Dutch horse breeds with different levels of inbreeding

A high degree of inbreeding has been reported to negatively impact semen quality in Friesian horses and Shetland ponies. Both breeds are characterized by a closed studbook, small population size, and high incidence of inbreeding. The Dutch Warmblood studbook (KWPN: Koninklijk Warmblood Paardenstamboek Nederland) is a much larger studbook with two distinct populations: the KWPN-Riding horses, managed as an 'open' studbook, and the KWPN-Harness horses, representing a much smaller subpopulation within the KWPN breed and managed as an 'almost closed' studbook. It was recently reported that the degree of inbreeding in KWPN-Harness horses has increased in recent decades due to the small gene pool; however, the degree of inbreeding is still lower than that of Friesian horses and Shetland ponies. We hypothesized that a high or rising degree of inbreeding might negatively impact semen quality. In the present study, we retrospectively compared semen quality parameters of stallions from four different breeds or types (Friesian Horses, Shetland Ponies, KWPN-Riding horses, and KWPN-Harness horses), each reported with different degrees of inbreeding. Semen concentration, and percentages of motile, morphologically normal and live spermatozoa, and the total number of morphologically normal, progressive motile spermatozoa per ejaculate (TNM) were analyzed for 2832 semen evaluations performed over a 15-year period. KWPN-Harness horses had a significantly lower sperm concentration, % motile spermatozoa and % live spermatozoa than KWPN-Riding horses but the % motile and % morphologically normal spermatozoa and TNM in both KWPN-Harness and KWPN-Riding horses were significantly higher than in Friesian horses and Shetland ponies. These results suggest a lower semen quality in KWPN-Harness than KWPN-Riding horses, potentially as a result of a higher coefficient of inbreeding. The negative trend observed in the KWPN-Harness horses may be a warning sign, and breeders or stud books should monitor the degree of inbreeding carefully to avoid a further reduction in semen quality, to the levels observed in Friesian horses and Shetland ponies

Initial testing of electrospun nanofibre filters in water filtration applications

The aim of this study was to evaluate the use of nanofibre microfiltration membranes, spun by an innovative electrospinning technique, in water filtration applications. As such, this study bridges the gap between developments in electrospinning techniques for the production of flat-sheet membranes and the application of these membranes in water filtration. Three different applications were examined. Firstly, the use of the membrane (functionalised or non-functionalised) for the removal of pathogens was investigated. Secondly, the electrospun flat-sheet membranes were applied for wastewater treatment in a laboratory-scale submerged membrane bioreactor (MBR). In addition to these applications, physical properties such as clean water permeability (CWP) and strength were also examined. The tests showed that the electrospun membranes can be used for water filtration applications, but that further improvements are necessary before these membranes can be practically employed. In particular, the level of functionality and the properties of irreversible fouling require further research.Keywords: electrospinning, nanofibre, microfiltration, pathogen removal, MB