Ability of skin bacteria on the Panamanian frog species, Craugastor fitzingeri, to inhibit the fungal pathogen Batrachochytrium dendrobatidis

An emerging infectious disease caused by the fungus Batrachochytrium dendrobatidis (Bd) is leading to global amphibian declines and is threatening the biodiversity of amphibians. Bd susceptibility varies among individuals, species, and populations perhaps due to defensive mechanisms such as symbiotic skin microbes. Some species of amphibians such as Craugastor fitzingeri, a terrestrial frog native to Central America, continue to persist in Bd-positive environments in Panama. My study focused on identifying antifungal bacterial isolates and determining the culturability of the bacterial community on 15 individuals of C. fitzingeri. Morphologically distinct isolates were challenged against Bd in inhibition assays to determine an inhibition score for each isolate. The 16S rRNA sequences of all cultured isolates were aligned and grouped in Operational Taxonomic Units (OTUs). The relative abundance of cultured OTUs was compared to that of the entire bacterial community obtained with the culture-independent method. Over 80% of the individuals had at least one morphologically distinct Bd-inhibitory isolate at an inhibition score of 80% or greater. In comparing culturability, the cultured community was significantly more relatively abundant than the entire culture-independent community (Wilcoxon test: W=101130, p= 2.53e-14). Specifically, Cellulomonas, Comamonas testosteroni, and Acinetobacter johnsonii were highly relative abundant and were culturable. However, one relatively abundant species of Pseudomonas was not culturable. Additionally, I was able to culture 17.92% of the total relative abundance within the entire bacterial community. Identifying inhibitory isolates and the relative abundances of culturable OTUs are crucial steps to designing an ideal probiotic to potentially protect amphibian populations from Bd

Prelamin A Influences a Program of Gene Expression In Regulation of Cell Cycle Control

The A-type lamins are intermediate filament proteins that constitute a major part of the eukaryotic nuclear lamina—a tough, polymerized, mesh lining of the inner nuclear membrane, providing shape and structural integrity to the nucleus. Lamin A (LA) filaments also permeate the nucleoplasm, providing additional structural support, but also scaffolding numerous tethered molecules to stabilize, organize, and facilitate molecular interactions to accomplish critical functions of cellular metabolism. Over the past 2 decades, much attention has been focused on roles of LA in maintenance of nuclear structural integrity. Only since the late 1990s have scientists discovered the devastating effects of LA gene (LMNA) mutations, as they have associated hundreds of LMNA mutations to a large group of diseases, called laminopathies, with a broad spectrum of phenotypes, ranging from skeletal, muscular, and neurological defects, to defective lipid storage, to accelerated aging phenotypes in diseases called progerias. Recent advances demonstrate LA regulatory functions include cell signaling, cell cycle regulation, transcription, chromatin organization, viral egress, and DNA damage repair. Amidst the flurry of fascinating research, only recently have researchers begun to focus attention on the different isoforms that exist for LA, a precursor form among them. LA is initially synthesized as Prelamin A (PreA), and undergoes a series of modifications that truncate the protein to produce “mature” LA. Existence of the precursor form, and its complex maturation pathway, have puzzled researchers since their realization. With a pattern of expression related to cell cycle phase, we hypothesized a role for PreA in cell cycle control. To investigate, we have performed array studies to assess gene expression effects at the levels of transcript expression, protein expression, and phosphorylation modification status. Here, we present evidence for a PreA-mediated program of cell cycle regulatory gene and protein expression modulation. Implicated pathways include RB-E2F, p53, p27Kip1, FoxOs, p300, and the Cyclins, with additional evidence indicating a role for the Pin1 prolyl isomerase in mediating PreA regulation of the cell cycle

Markov chain Monte Carlo analysis of Bianchi VII_h models

We have extended the analysis of Jaffe et al. to a complete Markov chain Monte Carlo (MCMC) study of the Bianchi type ${\rm VII_h}$ models including a dark energy density, using 1-year and 3-year Wilkinson Microwave Anisotropy Probe (WMAP) cosmic microwave background (CMB) data. Since we perform the analysis in a Bayesian framework our entire inference is contained in the multidimensional posterior distribution from which we can extract marginalised parameter constraints and the comparative Bayesian evidence. Treating the left-handed Bianchi CMB anisotropy as a template centred upon the `cold-spot' in the southern hemisphere, the parameter estimates derived for the total energy density, `tightness' and vorticity from 3-year data are found to be: $\Omega_{tot} = 0.43\pm 0.04$, $h = 0.32^{+0.02}_{-0.13}$, $\omega = 9.7^{+1.6}_{-1.5}\times 10^{-10}$ with orientation $\gamma = {337^{\circ}}^{+17}_{-23}$). This template is preferred by a factor of roughly unity in log-evidence over a concordance cosmology alone. A Bianchi type template is supported by the data only if its position on the sky is heavily restricted. The low total energy density of the preferred template, implies a geometry that is incompatible with cosmologies inferred from recent CMB observations. Jaffe et al. found that extending the Bianchi model to include a term in $\Omega_{\Lambda}$ creates a degeneracy in the $\Omega_m - \Omega_{\Lambda}$ plane. We explore this region fully by MCMC and find that the degenerate likelihood contours do not intersect areas of parameter space that 1 or 3 year WMAP data would prefer at any significance above $2\sigma$. Thus we can confirm that a physical Bianchi ${\rm VII_h}$ model is not responsible for this signature.Comment: 8 pages, 10 figures, significant update to include more accurate results and conclusions to match version accepted by MNRA

Production of gaseous fuel by pyrolysis of municipal solid waste

Pilot plant tests were conducted on a simulated solid waste which was a mixture of shredded newspaper, wood waste, polyethylene plastics, crushed glass, steel turnings, and water. Tests were conducted at 1400 F in a lead-bath pyrolyser. Cold feed was deaerated by compression and was dropped onto a moving hearth of molten lead before being transported to a sealed storage container. About 80 percent of the feed's organic content was converted to gaseous products which contain over 90 percent of the potential waste energy; 12 percent was converted to water; and 8 percent remained as partially pyrolyzed char and tars. Nearly half of the carbon in the feed is converted to benzene, toluene and medium-quality fuel gas, a potential credit of over $25 per ton of solid waste. The system was shown to require minimal preprocessing and less sorting then other methods

Ion laser plasmas

The typical noble gas ion laser plasma consists of a high-current-density glow discharge in a noble gas, in the presence of a magnetic field. Typical CW plasma conditions are current densities of 100 to 2000 A/cm^2, tube diameters of 1 to 10 mm, filling pressures of 0.1 to 1.0 torr, and an axial magnetic field of the order of 1000 G. Under these conditions the typical fractional ionization is about 2 percent and the electron temperature between 2 and 4 eV. Pulsed ion lasers typically use higher current densities and lower operating pressures. This paper discusses the properties of ion laser plasmas, in terms of both their external discharge parameters and their internal ion and excited state densities. The effect these properties have on laser operation is explained. Many interesting plasma effects, which are important in ion lasers, are given attention. Among these are discharge nonuniformity near tube constrictions, extremely high ion radial drift velocities, wall losses intermediate between ambipolar diffusion and free fall, gas pumping effects, and radiation trapping. The current status of ion laser technology is briefly reviewed

Experimental studies on the impact properties of water ice

Experimental studies on the impact of ice particles at very low velocity were continued. These measurements have applications in the dynamics of Saturn's rings. Initially data were obtained on the coefficient of restitution for ice spheres of one radius of curvature. The type of measurements were expanded to include restitution data for balls with a variety of surfaces as well as sticking forces between ice particles. Significant improvements were made to this experiment, the most important being the construction of a new apparatus. The new apparatus consists of a smaller version of the disk pendulum and a stainless steel, double-walled cryostat. The apparatus has proved to be a significant improvement over the old one. Measurements can now be made at temperatures near 90 K, comparable to the temperature of the environment of Saturn's rings, and with much greater temperature stability. It was found that a roughened contact surface or the presence of frost can cause a much larger change in the restitution measure than the geometrical effect of the radius of curvature

A universal relationship between magnetization and local structure changes below the ferromagnetic transition in La_{1-x}Ca_xMnO_3; evidence for magnetic dimers

We present extensive X-ray Absorption Fine Structure (XAFS) measurements on La_{1-x}Ca_xMnO_3 as a function of B-field (to 11T) and Ca concentration, x (21-45%). These results reveal local structure changes (associated with polaron formation) that depend only on the magnetization for a given sample, irrespective of whether the magnetization is achieved through a decrease in temperature or an applied magnetic field. Furthermore, the relationship between local structure and magnetization depends on the hole doping. A model is proposed in which a filamentary magnetization initially develops via the aggregation of pairs of Mn atoms involving a hole and an electron site. These pairs have little distortion and it is likely that they pre-form at temperatures above T_c.Comment: 5 pages, 5 figures (1 with 2 parts) -- v2. new data added (updated figures); discussion expande

Earth-like sand fluxes on Mars

Strong and sustained winds on Mars have been considered rare, on the basis of surface meteorology measurements and global circulation models, raising the question of whether the abundant dunes and evidence for wind erosion seen on the planet are a current process. Recent studies showed sand activity, but could not determine whether entire dunes were moving—implying large sand fluxes—or whether more localized and surficial changes had occurred. Here we present measurements of the migration rate of sand ripples and dune lee fronts at the Nili Patera dune field. We show that the dunes are near steady state, with their entire volumes composed of mobile sand. The dunes have unexpectedly high sand fluxes, similar, for example, to those in Victoria Valley, Antarctica, implying that rates of landscape modification on Mars and Earth are similar