Effects of Leaf Removal on Fruit Quality of Wine Grapes Grown in Iowa

Leaf removal practices are common in many viticulture regions, leading to improved fruit quality. However, little research has been done on the benefits and challenges of leaf removal on cold-climate grape cultivars grown in the Upper Midwest that have high acidity. Potential benefits of leaf removal include increased canopy air circulation and sunlight exposure, decreased cluster disease, and improved flavor, pH, and titratable acidity (TA). Potential leaf removal challenges include fruit sunburn, fewer photosynthesizing leaves around clusters, production of fruit offflavors, and increased production cost due to labor-intensive leaf removal. The objective of this study was to determine if leaf removal around grape clusters is beneficial to improve fruit quality (soluble solids, pH, and TA) of grape cultivars grown in Iowa

Fine map of the Gct1 spontaneous ovarian granulosa cell tumor locus

The spontaneous development of juvenile-onset, ovarian granulosa cell (GC) tumors in the SWR/Bm (SWR) inbred mouse strain is a model for juvenile-type GC tumors that appear in infants and young girls. GC tumor susceptibility is supported by multiple Granulosa cell tumor (Gct) loci, but the Gct1 locus on Chr 4 derived from SWR strain background is fundamental for GC tumor development and uniquely responsive to the androgenic precursor dehydroepiandrosterone (DHEA). To resolve the location of Gct1 independently from other susceptibility loci, Gct1 was isolated in a congenic strain that replaces the distal segment of Chr 4 in SWR mice with a 47 × 10(6)-bp genomic segment from the Castaneus/Ei (CAST) strain. SWR females homozygous for the CAST donor segment were confirmed to be resistant to DHEA- and testosterone-induced GC tumorigenesis, indicating successful exchange of CAST alleles (Gct1(CA)) for SWR alleles (Gct1(SW)) at this tumor susceptibility locus. A series of nested, overlapping, congenic sublines was created to fine-map Gct1 based on GC tumor susceptibility under the influence of pubertal DHEA treatment. Twelve informative lines have resolved the Gct1 locus to a 1.31 × 10(6)-bp interval on mouse Chr 4, a region orthologous to human Chr 1p36.22. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00335-012-9439-6) contains supplementary material, which is available to authorized users

Spatial and temporal variability of freshwater discharge into the Gulf of Alaska

A study of the freshwater discharge into the Gulf of Alaska (GOA) has been carried out. Using available streamgage data, regression equations were developed for monthly flows. These equations express discharge as a function of basin physical characteristics such as area, mean elevation, and land cover, and of basin meteorological characteristics such as temperature, precipitation, and accumulated water year precipitation. To provide the necessary input meteorological data, temperature and precipitation data for a 40 year hind-cast period were developed on high-spatial-resolution grids using weather station data, PRISM climatologies, and statistical downscaling methods. Runoff predictions from the equations were found to agree well with observations. Once developed, the regression equations were applied to a network of delineated watersheds spanning the entire GOA drainage basin. The region was divided into a northern region, ranging from the Aleutian Chain to the Alaska/Canada border in the southeast panhandle, and a southern region, ranging from there to the Fraser River. The mean annual runoff volume into the northern GOA region was found to be 792 ± 120 km³ yr⁻¹. A water balance using MODIS-based evapotranspiration rates yielded seasonal storage volumes that were consistent with GRACE satellite-based estimates. The GRACE data suggest that an additional 57 ± 11 km³ yr⁻¹ be added to the runoff from the northern region, due to glacier volume loss (GVL) in recent years. This yields a total value of 849 ± 121 km³ yr⁻¹. The ease of application of the derived regression equations provides an accessible tool for quantifying mean annual values, seasonal variation, and interannual variability of runoff in any ungaged basin of interest

Parental transfer of the antimicrobial protein LBP/BPI protects Biomphalaria glabrata eggs against oomycete infections

Copyright: © 2013 Baron et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was funded by ANR (ANR-07-BLAN-0214 and ANR-12-EMMA-00O7-01), CNRS and INRA. PvW was financially supported by the BBSRC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Homology of SMP domains to the TULIP superfamily of lipid-binding proteins provides a structural basis for lipid exchange between ER and mitochondria

Mitochondria must uptake some phospholipids from the endoplasmic reticulum (ER) for the biogenesis of their membranes. They convert one of these lipids, phosphatidylserine, to phosphatidylethanolamine, which can be re-exported via the ER to all other cellular membranes. The mechanisms underlying these exchanges between ER and mitochondria are poorly understood. Recently, a complex termed ER–mitochondria encounter structure (ERMES) was shown to be necessary for phospholipid exchange in budding yeast. However, it is unclear whether this complex is merely an inter-organelle tether or also the transporter. ERMES consists of four proteins: Mdm10, Mdm34 (Mmm2), Mdm12 and Mmm1, three of which contain the uncharacterized SMP domain common to a number of eukaryotic membrane-associated proteins. Here, we show that the SMP domain belongs to the TULIP superfamily of lipid/hydrophobic ligand-binding domains comprising members of known structure. This relationship suggests that the SMP domains of the ERMES complex mediate lipid exchange between ER and mitochondria

Serum levels of cytokines and C-reactive protein in acute ischemic stroke patients, and their relationship to stroke lateralization, type, and infarct volume

There is increasing evidence that inflammation plays an important role in the progression of acute ischemic stroke (AIS). The primary aims of this study were to examine the serum levels of 13 cytokines, C-reactive protein (CRP), glucose, and hemoglobin in AIS patients, and their relationship to stroke lateralization, type, and infarct volume. Forty-five patients with AIS were evaluated. Blood samples were taken within 72 h, and volumetric analyses performed within 1–7 days after AIS onset. Cytokines were measured in serum from all patients and from 40 control subjects using Luminex Bio-Plex XMap technology. The levels of interleukin (IL)-1ra (p < 0.001), IL-6 (p < 0.001), IL-8 (p < 0.001), IL-9 (p = 0.038), IL-10 (p = 0.001), IL-12 (p = 0.001), IL-18 (p < 0.001), and GRO-α (CXCL1) (p = 0.017) were significantly higher in the AIS patients than in the controls. The IL-8 level was significantly correlated with age in the patient group (r = 0.52, p < 0.001). None of the variables were found to be associated with stroke lateralization. Infarct volume was significantly positively correlated with CRP level (r = 0.47, p = 0.005). Patients with radiologically confirmed infarctions had significantly elevated serum levels of GRO-α (p = 0.023). The cytokine profile of the AIS patients supports not only earlier findings of a proinflammatory response but also early activation of endogenous immunosuppressive mechanisms. Novel findings of this study are elevated serum levels of IL-9 and GRO-α. Elevated GRO-α in AIS patients with radiologically confirmed infarctions suggests that GRO-α is specific for stroke of known etiology. Our results indicate that CRP plays an important role in the progression of cerebral tissue injury