Shaping Arctic’s Tomorrow through Indigenous Knowledge Engagement and Knowledge Co-Production

This perspective presents a statement of the 10th International Congress of Arctic Social Sciences Indigenous Knowledge and knowledge co-production panel and discussion group, 20 July 2021. The statement is designed to serve as a characterization of the state-of-the-art and guidance for further advancement of Indigenous Knowledge and knowledge co-production in the Arctic. It identifies existing challenges and provides specific recommendations for researchers, Indigenous communities, and funding agencies on meaningful recognition and engagement of Indigenous Knowledge systems

A roadmap for Antarctic and Southern Ocean science for the next two decades and beyond

Antarctic and Southern Ocean science is vital to understanding natural variability, the processes that govern global change and the role of humans in the Earth and climate system. The potential for new knowledge to be gained from future Antarctic science is substantial. Therefore, the international Antarctic community came together to ‘scan the horizon’ to identify the highest priority scientific questions that researchers should aspire to answer in the next two decades and beyond. Wide consultation was a fundamental principle for the development of a collective, international view of the most important future directions in Antarctic science. From the many possibilities, the horizon scan identified 80 key scientific questions through structured debate, discussion, revision and voting. Questions were clustered into seven topics: i)Antarctic atmosphere and global connections, ii) Southern Ocean and sea ice in a warming world, iii) ice sheet and sea level, iv) the dynamic Earth, v) life on the precipice, vi) near-Earth space and beyond, and vii) human presence in Antarctica. Answering the questions identified by the horizon scan will require innovative experimental designs, novel applications of technology, invention of next-generation field and laboratory approaches, and expanded observing systems and networks. Unbiased, non-contaminating procedures will be required to retrieve the requisite air, biota, sediment, rock, ice and water samples. Sustained year-round access toAntarctica and the Southern Ocean will be essential to increase winter-time measurements. Improved models are needed that represent Antarctica and the Southern Ocean in the Earth System, and provide predictions at spatial and temporal resolutions useful for decision making. A co-ordinated portfolio of cross-disciplinary science, based on new models of international collaboration, will be essential as no scientist, programme or nation can realize these aspirations alone.Tinker Foundation, Antarctica New Zealand, The New Zealand Antarctic Research Institute, the Scientific Committee on Antarctic Research (SCAR), the Council of Managers of National Antarctic Programs (COMNAP), the Alfred Wegner Institut, Helmholtz Zentrum für Polar und Meeresforschung (Germany), and the British Antarctic Survey (UK).http://journals.cambridge.org/action/displayJournal?jid=ANShb201

Urokinase: Isolation, Purification, Characterization, New Spectrophotometric Bioassay Method and In-vitro Blood Clot Dissolving Activity from Cow Urine

Urokinase, a potent plasminogen activator is an enzyme present in cowurine attributing to the thromboembolic properties of cow urine but its presence in cow urine has never been convincingly documented. In our work, we did isolation, purification, partial characterization and quantitation of urokinase in cowurine using newly developed spectrophotometric bioassay method and studied its invitro blood clot dissolving activity. The cow urine was collected from cows of Indian origin (Bos indicus). Isolation of Urokinase was done by ammonium sulfate precipitation and dialysis. Purification was done by using Sephadex G-200 Gel Filtration Chromatography. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Gelatin Zymography, HPLC and TLC of tryptic digest of the purified urokinase was carried out for partial characterization studies which conformed the molecular weight of 59 kD of the purified Urokinase. Quantification with the help of new spectrophotometric bioassay method showed 956 IU/ml and with the help of HPLC it was 910 IU/ml of urokinase in cow urine. The invitro blood clot dissolving activity on a cotton cloth confirmed the activity and efficacy of the isolated and purified urokinase

The action of red scorpion (Mesobuthus tamulus coconsis, pocock) venom and its isolated protein fractions on blood sodium levels

Red scorpion (Mesobuthus tamulus or Buthus tamulus) venom samples were collected at different regions of India: western (Chiplun and Ahmednagar from Maharashtra State) and southern (Ratnagiri and Chennai from Tamil Nadu State). The action of whole venoms on the blood sodium levels of mice was assessed using flame photometry. Seven peptides were common to all venom samples. They were separated using the native polyacrylamide gel electrophoresis (PAGE) technique and their activities were also studied using flame photometry. There was a decrease in the concentration of sodium ions in the serum, which suggested the blockage of such ions by scorpion venom toxins. Among the 10 protein bands isolated, the band at 79.6 kDa presented maximum activity in decreasing serum sodium ions concentration. Whole venom from Chiplun region also showed maximum activity. The western blotting technique demonstrated that the anti-scorpion venom sera produced by Haffkine Biopharmaceuticals Corporation Ltd., India, neutralized all four venom samples

Effective carbon and nutrient treatment solutions for mixed domestic-industrial wastewater in India

The present study evaluates effectiveness of up-flow anaerobic sludge blanket (UASB) reactor followed by two post-anaerobic treatment options, namely free-surface, up-flow constructed wetland (FUP-CW) and oxygen-limited anaerobic nitrification/denitrification (OLAND) processes in treating sewage from the peri-urban areas in India receiving illegal industrial infiltrations. The UASB studies yielded robust results towards fluctuating strength of sewage and consistently removed 87-98% chemical oxygen demand (COD) at a hydraulic retention time of 1.5-2 d. The FUP-CW removed 68.5 +/- 13% COD, 68 +/- 3% NH4+-N, 38 +/- 5% PO43--P, 97.6 +/- 5% suspended particles and 97 +/- 13% fecal coliforms. Nutrient removal was found to be limiting in FUP-CW, especially in winter. Nitrogen removal in the OLAND process were 100 times higher than the FUP-CW process. Results show that UASB followed by FUP-CW can be an excellent, decentralized sewage treatment option, except during winter when nutrient removal is limited in FUP-CW. Hence, the study proposes bio-augmentation of FUP-CW with OLAND biomass for overall improvement in the performance of UASB followed by FUP-CW process