Proceedings of the Thirteenth International Society of Sports Nutrition (ISSN) Conference and Expo

Meeting Abstracts: Proceedings of the Thirteenth International Society of Sports Nutrition (ISSN) Conference and Expo Clearwater Beach, FL, USA. 9-11 June 201

Transition metal transport

Transition metal transporters are of central importance in the plant metal homeostasis network which maintains internal metal concentrations within physiological limits. An overview is given of the functions of known transition metal transporters in the context of the unique chemical properties of their substrates. The modifications of the metal homeostasis network associated with the adaptation to an extreme metalliferous environment are illustrated in two Brassicaceae metal hyperaccumulator model plants based on cross-species transcriptomics studies. In a comparison between higher plants and unicellular algae, hypotheses are generated for evolutionary changes in metal transporter complements associated with the transition to multicellularity. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved

Circadian Life Without Micronutrients: Effects of Altered Micronutrient Supply on Clock Function in Arabidopsis

The plant circadian clock is formed by a number of interlocked feedback loops that control the expression of thousands of genes. Genetic and pharmacological approaches towards the study of the plant clock are routinely carried out on Murashige and Skoog growth medium, which is both Fe-replete and Cu-deficient. However, it has recently become clear that the plant clock responds to available iron (Fe) supply: circadian pace slows down under conditions of Fe deficiency; circadian period progressively shortens with increasing Fe supply. Here, we describe several growth media that may be used to study the effects of varying micronutrient supply on the circadian clock, in which deficiency in a given micronutrient are imposed by the addition of a specific chelator or, alternatively, by using EDTA-washed agar as gelling agent, thus minimizing micronutrient contamination