Natural resistance to snake venoms

La resistencia natural de algunos animales a los venenos de serpientes se ha logrado demostrar en el suero sanguíneo de mamíferos (Zarigüeyas, erizos, mangostas) y de serpientes. En este artículo se presenta el estado actual del conocimiento acerca de estas proteínas que no son anticuerpos, los métodos de caracterización y los mecanismos de acción de las mismas.The natural resistance of some animals to snake venoms has been demonstrated in the blood serum of mammals (possums, hedgehogs, mongooses) and snakes. This article presents the current state of knowledge about these non-antibody proteins, their characterization methods, and their mechanisms of action

Maintenance of red-tail coral snake (Micrurus mipartitus) in captivity and evaluation of individual venom variability

Red-tail coral snake (Micrurus mipartitus) is a long and thin bicolor coral snake widely distributed in Colombia and is the coral that causes the majority of accidents in the Andean region, so it is important to keep this species in captivity for anti-venom production and research. However, maintaining this species in captivity is very difficult because it refuses to feed, in addition to the high mortality rate due to maladaptation syndrome. In this study a force feeding diet, diverse substrates for maintenance and a milking technique were evaluated. Additionally, individual variability of the venom was determined by High Performance Liquid Chromatography (HPLC), Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coagulant, Anticoagulant and Hemolytic activities. The results of this study demonstrate that it was possible to increase the survival rate of this species in captivity and to determine some of the important factors in the maintenance. As to the individual variability of the venom, we found differences in number and intensity of peaks recovered by chromatography and also displayed variations in some of its biological activities.La coral “rabo de ají” es una coral bicolor larga y delgada. Esta especie está ampliamente distribuida en Colombia y es la coral que causa el mayor número de accidentes en la región Andina, por esto es importante mantener esta especie en cautiverio con fines de producción de antivenenos e investigación. No obstante, el mantenimiento de esta especie en cautiverio es difícil, debido a que se rehúsan a alimentarse voluntariamente y a que presentan alta mortalidad por el denominado síndrome de mal adaptación. En este estudio se evaluaron varios  sustratos para el mantenimiento, además de una dieta forzada y una técnica de ordeño. Adicionalmente, se evaluó la variabilidad individual del veneno a través de cromatografía liquida de alta eficiencia (HPLC), electroforesis (SDS-PAGE) y las actividades coagulante, anticoagulante y hemolítica indirecta. Los resultados de este estudio demostraron que fue posible incrementar la sobrevivencia de esta especie en cautiverio, así como determinar algunos factores importantes en su mantenimiento. A partir de la evaluación del veneno se encontraron diferencias en el número y en la intensidad de picos en la cromatografía, así como en algunas de sus actividades biológicas

Aspectos toxinológicos , clínicos y epidemiológicos del envenenamiento producido por el escorpión Tityus fuhrmanni Kraepelin

ResumenCon el objetivo de determinar las características toxinológicas, clínicas y epidemiológicas del envenenamiento producido por el escorpión T. fuhrmanni, se realizó durante un año un estudio prospectivo en un sector de la ciudad de Medellin(Cerro El volador y los barrios aledaños San germán y El Volador), en una muestra de 9,6% de las casas(180) y de 9,4% de la población del área (719 habitantes). Se incluyó también la recolección de especímenes vivos (128) y de veneno para la parte experimental.[Gómez JP, Otero R, Núñes V, Saldarriaga M, Díaz A, Velásques P. Aspectos toxinológicos , clínicos y epidemiológicos del envenenamiento producido por el escorpión Tityus fuhrmanni Kraepelin. MedUNAB 2002; 5(15):159-65].Palabras clave: Escorpión, Tityus fuhrmanni, envenenamiento, clínica, epidemiológia.&nbsp

Effect of Agraz (Vaccinium meridionale Swartz) on high-density lipoprotein function and inflammation in women with metabolic syndrome

ABSTRACT: Metabolic syndrome (MetS) is associated with low-grade inflammation and high-density lipoprotein (HDL) dysfunction. Polyphenol-rich foods may improve these alterations. Agraz is a fruit rich in polyphenols (mainly anthocyanins); however, there is limited information about its effects on human health. We evaluated the effects of agraz consumption as compared to placebo on HDL function and inflammation in women with MetS. Forty volunteers (25–60 years) were included in this double-blind crossover study. Women consumed agraz or placebo over 4 weeks; separated by a 4-week washout period. HDL function (apoliprotein-A1; paraoxonase 1 (PON1) activity; cholesterol efflux capacity), oxidative stress (myeloperoxidase (MPO), advanced oxidation protein products) and inflammatory markers (serum cytokines/chemokines and peripheral blood mononuclear cell nuclear factor-kB) were measured after each period. Compared to placebo, agraz consumption did not significantly change any of the biomarkers measured. Interestingly, only after agraz period there were significant positive correlations between PON1 activities and cholesterol efflux. Additionally, there were significant inverse correlations between changes in inflammatory markers and HDL function markers and positive correlations with oxidative markers. Although polyphenol-rich foods have been shown to be beneficial for certain conditions; polyphenol-rich agraz fruit consumption did not impact inflammation and HDL function in the current study of women with MetS

Physico-chemical characterization and antioxidant capacity of the colombian berry (Vaccinium meridionale Swartz) with a high-polyphenol content: potential effects in people with metabolic syndrome

ABSTRACT: Colombian berry (agraz) has demonstrated a high antioxidant content in vitro. In the present study, it was elaborated and characterized an agraz nectar, designed a placebo with similar physicochemical and sensorial characteristics of the agraz nectar, but without polyphenols; and evaluated their antioxidant capacity both in vitro and in vivo in adults with cardiovascular risk factors. The polyphenolic concentration of the agraz nectar and its antioxidant capacity was superior than other reported for this fruit. A high content of total cyanidins was observed in the freeze-dried agraz, but not delphinin was detected by HPLC. For the intervention study, sixty-six subjects, with at least three cardiometabolic risk factors, consumed agraz nectar or placebo daily during 4 weeks each, separated by a 4-week washout period. Total phenol concentration and antioxidant capacity in the participants’ serum were not statistically different after consuming agraz nectar and placebo. However, women increased significantly their serum DPPH scavenging capacity after consuming agraz nectar, compared to placebo. This is one of the first studies showing data about the effects of this colombian berry on the antioxidant capacity in people with metabolic syndrome

Toxinological, clinical and epidemiological aspects of the envenomation produced by the scorpion Tityus fuhrmanni Kraepelin

Con el objetivo de determinar las características toxinológicas, clínicas y epidemiológicas del envenenamiento producido por el escorpión T. fuhrmanni, se realizó durante un año un estudio prospectivo en un sector de la ciudad de Medellín (cerro El Volador y los barrios aledaños San Germán y El Volador), en una muestra del9,6% de las casas (180) y del 9,4% de la población del área (719 habitantes). Se incluyó también la recolección de especímenes vivos (128) y de veneno para la parte experimental. La dispersión de escorpiones para el área de estudio fue del 100%, con alta infestación domiciliaria en las viviendas del cerro y del barrio San Germán (100% y 32,3%, respectivamente). Durante todo el estudio se detectaron 32 accidentes por T.fuhrmanni, 7 (21,9%) en niños, 15 en el cerro y 10 en el barrio San Germán, con tasas de ataque del 83% y del 3,9%, respectivamente. Dieciocho (56,3%) accidentes ocurrieron en el interior de las viviendas, principalmente en las manos (31,3%), la cabeza y el cuello (18,8%) y durante la noche o en las primeras horas de la mañana (62,5%). El 90,6% de los casos fueron leves y el 9,4% (3 niños) tuvieron envenenamiento moderado con signos sistémicos (sudoración generalizada, dolor abdominal). No hubo casos graves y los pacientes no requirieron hospitalización. Tityus fuhrmanni produjo 0,56 ± 0,27 mg de veneno por ordeño, ysu DL50 (i.p.) en ratones (18-20 g) fue 79,2 μg (3,9 mg veneno/kg). Los animales presentaron sialorrea, piloerección, somnolencia, sudoración generalizada, taquipnea, cianosis, ataxia y convulsiones antes de morir. La rápida aparición (10-15 min) de los signos de envenenamiento en los ratones y su pronta desaparición(2 horas) en los sobrevivientes, permiten concluir que el veneno es de rápida absorción, distribución y eliminación. [Gómez JP, Otero R, Núñez V, Saldarriaga M, Díaz A, Velásquez P. Aspectos toxinológicos, clínicos y epidemiológicos del envenenamiento producido por el escorpión Tityus fuhrmanni Kraepelin.MEDUNAB 2002; 5(15): 159-65]In order to determine the toxinological, clinical and epidemiological characteristics of the poisoning produced by the scorpion T. fuhrmanni, a prospective study was carried out for a year in a sector of the city of Medellín (Cerro El Volador and the neighboring neighborhoods San Germán and El Volador), in a sample of 9.6% of the houses (180) and 9.4% of the population of the area (719 inhabitants). The collection of live specimens (128) and poison for the experimental part was also included. The dispersion of scorpions for the study area was 100%, with high home infestation in the homes of the hill and the San Germán neighborhood (100% and 32.3%, respectively). Throughout the study, 32 accidents were detected by T. fuhrmanni, 7 (21.9%) in children, 15 in the hill and 10 in the San Germán neighborhood, with attack rates of 83% and 3.9%, respectively. . Eighteen (56.3%) accidents occurred inside homes, mainly in the hands (31.3%), head and neck (18.8%) and during the night or in the early hours of the morning (62.5%). 90.6% of the cases were mild and 9.4% (3 children) had moderate poisoning with systemic signs (generalized sweating, abdominal pain). There were no serious cases and the patients did not require hospitalization. Tityus fuhrmanni produced 0.56 ± 0.27 mg of venom per milking, and its LD50 (i.p.) in mice (18-20 g) was 79.2 μg (3.9 mg venom / kg). The animals presented with salivation, piloerection, drowsiness, generalized sweating, tachypnea, cyanosis, ataxia and seizures before dying. The rapid appearance (10-15 min) of the poisoning signs in the mice and their prompt disappearance (2 hours) in the survivors, allow us to conclude that the venom is of rapid absorption, distribution and elimination. [Gómez JP, Otero R, Núñez V, Saldarriaga M, Díaz A, Velásquez P. Toxinological, clinical and epidemiological aspects of the poisoning produced by the scorpion Tityus fuhrmanni Kraepelin.MEDUNAB 2002; 5 (15): 159-65

Neutralizing capacity of a new monovalent anti-Bothrops atrox antivenom: Comparison with two commercial antivenoms

Three horse-derived antivenoms were tested for their ability to neutralize lethal, hemorrhagic, edema-forming, defibrinating and myotoxic activities induced by the venom of Bothrops atr-ox from Antioquia and Choco (Colombia). The following antivenoms were used: a) polyvalent (crotaline) antivenom produced by Institute Clodomiro Picado (Costa Rica), b) monovalent antibothropic antivenom produced by Institute Nacional de Salud-INS (Bogota), and c) a new monovalent anti-B. atrox antivenom produced with the venom of B. atrox from Antioquia and Choco. The three antivenoms neutralized all toxic activities tested albeit with different potencies. The new monovalent anti-B. atrox antivenom showed the highest neutralizing ability against edema-forming and defibrinating effects of B. atrox venom (41 +/- 2 and 100 +/- 32 mu l antivenom/mg venom, respectively), suggesting that it should be useful in the treatment of B. atrox envenomation in Antioquia and Choco.Universidad de Costa Rica//UCR/Costa RicaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP

Resistencia natural a los venenos de serpientes

ResumenLa resistencia natural de algunos animales a los venenos de serpientes se ha logrado demostrar en el suero sanguíneo de mamiferos (Zarigueyas, erizos, mangostas) y de serpientes. En este artículo se presenta el estado actual del conocimiento acerca de estas proteínas que no son anticuerpos, los métodos de caracterización y los mecanismos de acción de las mismas.Palabras clave: Venenos de serpientes, Hemorragias, Neurotoxinas, Miotoxinas, Resistencia natural

Anti-Neurotoxins from <i>Micrurus mipartitus</i> in the Development of Coral Snake Antivenoms

In Colombia, the genus Micrurus includes 30 species, of which M. mipartitus and M. dumerilii are the most widely distributed. Micrurus causes less than 3% of the approximately 5000 cases of snakebite per year. The elapid envenomation caused by the snakes from the Micrurus genus, are characterized by the severity of their clinical manifestations, due to the venom neurotoxic components such as three-finger toxins (3FTx) and phospholipases (PLA2). The treatment for snakebites is the administration of specific antivenoms, however, some of them have limitations in their neutralizing ability. A strategy proposed to improve antivenoms is to produce antibodies against the main components of the venom. The aim of this work was to produce an antivenom, using an immunization protocol including the main 3FTx and PLA2 responsible for M. mipartitus lethality. The antibody titers were determined by ELISA in rabbits’ serum. The immunized animals elicited a response against toxins and whole venom. The Immunoglobulin G (IgGs) obtained were able to neutralize the lethal effect of their homologous toxins. A combination of antivenom from M. mipartitus with antitoxins improved their neutralizing ability. In the same way, a mixture of anti 3FTx and PLA2 protected the mice from a 1.5 median lethal dose (LD50) of M. mipartitus venom. The results showed that this might be a way to improve antibody titers specificity against the relevant toxins in M. mipartitus venom and indicated that there is a possibility to develop and use recombinant 3FTx and PLA2 toxins as immunogens to produce antivenoms. Additionally, this represents an alternative to reduce the amount of venom used in anti-coral antivenom production