Preparation and characterization of magnetite (Fe3O4) nanoparticles By Sol-Gel method

The magnetite (Fe3O4) nanoparticles were successfully synthesized and annealed under vacuum at different temperature. The Fe3O4 nanoparticles prepared via sol-gel assisted method and annealed at 200-400ºC were characterized by Fourier Transformation Infrared Spectroscopy (FTIR), X-ray Diffraction spectra (XRD), Field Emission Scanning Electron Microscope (FESEM) and Atomic Force Microscopy (AFM). The XRD result indicate the presence of Fe3O4 nanoparticles, and the Scherer`s Formula calculated the mean particles size in range of 2-25 nm. The FESEM result shows that the morphologies of the particles annealed at 400ºC are more spherical and partially agglomerated, while the EDS result indicates the presence of Fe3O4 by showing Fe-O group of elements. AFM analyzed the 3D and roughness of the sample; the Fe3O4 nanoparticles have a minimum diameter of 79.04 nm, which is in agreement with FESEM result. In many cases, the synthesis of Fe3O4 nanoparticles using FeCl3 and FeCl2 has not been achieved, according to some literatures, but this research was able to obtained Fe3O4 nanoparticles base on the characterization results

Star formation associated with neutral hydrogen in the outskirts of early-type galaxies

About 20 percent of all nearby early-type galaxies ($M_{\star} \gtrsim 6 \times 10^{9}$ M$_{\odot}$) outside the Virgo cluster are surrounded by a disc or ring of low-column-density neutral hydrogen (HI) gas with typical radii of tens of kpc, much larger than the stellar body. In order to understand the impact of these gas reservoirs on the host galaxies, we analyse the distribution of star formation out to large radii as a function of HI properties using GALEX UV and SDSS optical images. Our sample consists of 18 HI-rich galaxies as well as 55 control galaxies where no HI has been detected. In half of the HI-rich galaxies the radial UV profile changes slope at the position of the HI radial profile peak. To study the stellar populations, we calculate the FUV-NUV and UV-optical colours in two apertures, 1-3 and 3-10 R$_{eff}$ . We find that HI -rich galaxies are on average 0.5 and 0.8 mag bluer than the HI-poor ones, respectively. This indicates that a significant fraction of the UV emission traces recent star formation and is associated with the HI gas. Using FUV emission as a proxy for star formation, we estimate the integrated star formation rate in the outer regions (R > 1R$_{eff}$) to be on average $6 \times 10^{-3}$ M$_{\odot}$ yr$^{-1}$ for the HI-rich galaxies. This rate is too low to build a substantial stellar disc and, therefore, change the morphology of the host. We find that the star formation efficiency and the gas depletion time are similar to those at the outskirts of spirals.Comment: 27 pages (13 without appendices). 9 figures, 5 tables, 2 appendix tables and 12 appendix figures. Accepted for publication in MNRA

The neonatal splice variant of Nav1.5 potentiates in vitro invasive behaviour of MDA-MB-231 human breast cancer cells

Upregulation of functional voltage-gated Na+ channels (VGSCs) occurs in metastatic human breast cancer (BCa) in vitro and in vivo. The present study aimed to ascertain the specific involvement of the 'neonatal' splice variant of Nav1.5 (nNav1.5), thought to be predominant, in the VGSC-dependent invasive behaviour of MDA-MB-231 cells. Functional activity of nNav1.5 was suppressed by two different methods targeting nNav1.5: (i) small interfering RNA (siRNA), and (ii) a polyclonal antibody (NESO-pAb); effects upon migration and invasion were determined. nNav1.5 mRNA, protein and signalling were measured using real-time PCR, Western blotting, and patch clamp recording, respectively. Treatment with the siRNA rapidly reduced (by similar to 90%) the level of nNav1.5 (but not adult Nav1.5) mRNA, but the protein reduction was much smaller (similar to 30%), even after 13 days. Nevertheless, the siRNA reduced peak VGSC current density by 33%, and significantly increased the cells' sensitivity to nanomolar tetrodotoxin (TTX). Importantly, the siRNA suppressed in vitro migration by 43%, and eliminated the normally inhibitory effect of TTX. Migrated MDA-MB-231 cells expressed more nNav1.5 protein at the plasma membrane than non-migrated cells. Furthermore, NESO-pAb reduced migration by up to 42%, in a dose-dependent manner. NESO-pAb also reduced Matrigel invasion without affecting proliferation. TTX had no effect on cells already treated with NESO-pAb. It was concluded that nNav1.5 is primarily responsible for the VGSC-dependent enhancement of invasive behaviour in MDA-MB-231 cells. Accordingly, targeting nNav1.5 expression/activity may be useful in clinical management of metastatic BCa

Advanced Electrodes for Solid Acid Fuel Cells by Platinum Deposition on CsH_(2)PO_4

We demonstrate cathodes for solid acid fuel cells fabricated by vapor deposition of platinum from the metalorganic precursor Pt(acac)_2 on the solid acid CsH_(2)PO_4 at 210 °C. A network of platinum nanoparticles with diameters of 2−4 nm serves as both the oxygen reduction catalyst and the electronic conductor in the electrode. Electrodes with a platinum content of 1.75 mg/cm^2 are more active for oxygen reduction than previously reported electrodes with a platinum content of 7.5 mg/cm^2. Electrodes containing <1.75 mg/cm^2 of platinum show significantly reduced catalytic activity and increased ohmic resistance indicative of a highly discontinuous catalytic-electronic platinum network