The draft genome sequence of Xanthomonas species strain Nyagatare, isolated from diseased bean in Rwanda.

types: Journal ArticleThis is a pre-copyedited, author-produced PDF of an article accepted for publication in FEMS following peer review. The version of record Aritua, V., Musoni, A., Kabeja, A., Butare, L., Mukamuhirwa, F., Gahakwa, D., . . . Smith, J. (2015). The draft genome sequence of Xanthomonas species strain Nyagatare, isolated from diseased bean in Rwanda, FEMS Microbiology Letters, 2015, Vol. 362, No. 4 pp. 1-4 is available online at: http://femsle.oxfordjournals.org/content/362/4/1.1.exploreWe announce the genome sequence for Xanthomonas species strain Nyagatare, isolated from beans showing unusual disease symptoms in Rwanda. This strain represents the first sequenced genome belonging to an as-yet undescribed Xanthomonas species known as species-level clade 1. It has at least 100 kb of genomic sequence that shows little or no sequence similarity to other xanthomonads, including a unique lipopolysaccharide synthesis gene cluster. At least one genomic region appears to have been acquired from relatives of Agrobacterium or Rhizobium species. The genome encodes homologues of only three known type-three secretion system effectors: AvrBs2, XopF1 and AvrXv4. Availability of the genome sequence will facilitate development of molecular tools for detection and diagnostics for this newly discovered pathogen of beans and facilitate epidemiological investigations of a potential causal link between this pathogen and the disease outbreak.Canadian International Development AgencyBBSRC SCPRI

Pattern and clinical management of penile cancer in Rwanda

Introduction: Penile cancer is rare in developed countries but has a high prevalence in some developing countries. Surgery includes inguinal lymphadenectomy, which remains the mainstay treatment of the disease.Objective: This study reports on the epidemiological profile of penile cancer and clinical management options in Rwanda. Patients and methods: From January 2015 to June 2016, a multicenter cross-sectional, prospective cohort study was conducted involving all male patients presenting with penile cancer after two national radio campaigns and a Ministry of Health instruction to all district hospitals. All patients with positive biopsy were included. Surgical treatment aligned with published guidelines. Clinical characteristics, surgery, pathology, and early follow-up data were collected.Results: Over 18 consecutive months, 30 male patients were enrolled. The mean age was 60 years [range 33–83]. All patients were uncircumcised before symptom onset; 50% had phimosis and 20% were HIV- positive. The estimated prevalence of penile cancer in Rwanda was 0.37 per 100,000 men. At presentation, 96.7% of patients had a T2-4 disease and 43.3% were with clinically non-palpable inguinal lymph nodes (cNO). After penectomy, bilateral inguinal lymphadenectomy was performed in 10 (33.3%) patients (modified and radical in 16 and 4 limbs, respectively). Complications included surgical site infection (10%), lymphocele (10%), urethral meatus stenosis (6.7%), skin necrosis (3.3%) and two (6.7%) patients with metastatic disease died in hospital.Conclusion: Penile cancer is a rare but significant disease in Rwanda. Patients present with advanced disease. After treatment of the primary tumor, modified inguinal lymphadenectomy appears to be a safe method of cure and staging for patients with clinically impalpable inguinal lymph nodes. Our early results provide a compelling insight into this rare but serious disease

Rwanda Nutrition, Markets and Gender Analysis 2015: An integrated approach towards alleviating malnutrition among vulnerable populations in Rwanda

The Nutrition, Markets and Gender (NMG) Survey was conducted in Rwanda to investigate the causes of malnutrition in children under 24 months. The NMG Survey was informed by the 2010 Demographic Health Survey (DHS) for Rwanda that gave some insight into the knowledge and trend of malnutrition in the country for the period 2005 to 2010. The DHS results indicated a 6 percent decline in stunting among children under the age of five years. The key findings from the 2014/2015 DHS that followed the same delineation as the DHS 2010 provided the most current status of malnutrition in Rwanda and showed further overall improvements in child growth outcomes with 37.9 percent of children under five years classified as stunted. These results again indicated a 6.3 percent decline in stunting among children under the age of five years for the period 2010 to 2015. This progressive trend is a testament to the country’s commitments to prioritise nutrition issues and nutrition programmes in its development agenda. The Government of Rwanda, through the Ministry of Health, has prioritised malaria control, nutrition education, and better public healthcare. However, in spite of the advancements made, the consensus is that high rates of chronic malnutrition among children still prevail. Thus a better understanding of risk factors that contribute to child malnutrition at the household level in Rwanda was needed to strengthen the fight against malnutrition in the country. The ‘Comprehensive Food Security and Vulnerability and Nutrition Analysis’ – CFSVA 2012 – report produced by the Ministry of Agriculture and Animal Resources (MINAGRI), the National Institute of Statistics of Rwanda (NISR) and the World Food Programme (WFP) acknowledged that vulnerable households are increasingly reliant on markets as a source of food; providing on average 65 percent of the food consumed by a household. It is therefore clear that household nutrition outcomes in such households are dependent on markets. In addition, it is widely accepted that gender dynamics influence decision-making in the household. Thus gender dynamics affect decisions related to food, care, markets, and health. Therefore this survey focused on nutrition, markets, and gender to determine the factors that influence the nutrition status of children under 24 months. Moreover, a disconnect between agricultural production and nutrition outcomes was revealed in the CFSVA 2012 report that indicated that the northern agricultural zones, considered the bread basket of the country, had stunting rates of up to 66 percent in children under 60 months

Assessing the risk of root rots in common beans in East Africa using simulated, estimated and observed daily rainfall data

This paper seeks to establish the concept that the analysis of high temporal resolution meteorological data adds value to the investigation of the effect of climatic variability on the prevalence and severity of agricultural pests and diseases. Specifically we attempt to improve disease potential maps of root rots in common beans, based on a combination of inherent susceptibility and the risk of exposure to critical weather events. We achieve this using simulated datasets of daily rainfall to assess the probability of heavy rainfall events at particular times during the cropping season. We then validate these simulated events with observations from meteorological stations in East Africa. We also assess the utility of remotely sensed daily rainfall estimates in near real time for the purposes of updating the risks of these events over large areas and for providing warnings of potential disease outbreaks. We find that simulated rainfall data provide the means to assess risk over large areas, but there are too few datasets of observed rainfall to definitively validate the probabilities of heavy rainfall events generated using rainfall simulations such as those generated by MarkSim. We also find that selected satellite rainfall estimates are unable to predict observed rainfall events with any power, but data from a sufficiently dense network of rain gauges are not available in the region. Despite these problems we show that remotely sensed rainfall estimates may provide a more realistic assessment of rainfall over large areas where rainfall observations are not available, and alternative satellite estimates should be explored. © 2011 Cambridge University Press

Inheritance of fusarium wilts (Fusarium oxysporum F. sp. phaseoli) resistance in climbing beans

Fusarium wilt, caused by Fusarium oxysporum F. sp. phaseoli caused growers to abandon the most popular climbing bean cultivar, Umubano (G2333) in Rwanda. The present objective was to determine the nature of inheritance of fusarium wilt resistance and recommend a breeding strategy to introduce resistance into susceptible cultivars. Two cultivars, vuninkingi (G685) and flora were donors of resistance to fusarium wilt whereas G2333 was highly susceptible. Injured root tips of 10-day old seedlings of the parents, progenies of F1 and F2 (G2333 × G685) and (G2333 × Flora), backcrosses F2 (G685 × Flora) were inoculated with 106 conidia ml-1 of Rwandan isolate of F. oxysporum F. sp phaseoli (FOP-RW2) in a glasshouse. The disease severity was rated 28 days later using the CIAT scale of 1 - 9, where 1 - 3 represent resistant, 4 - 6 tolerant and 7 - 9 susceptible reactions. The chi-square analysis was performed to determine the Mendelian segregation ratios of resistant and susceptibility among the inoculated progenies. The F1 and the backcross progenies to the resistant parents segregated in the ratio of 1:0 as did the F2 population (G685 × Flora). The F2 progenies segregated in the ratio of 3:1. The backcross progenies to the susceptible parent segregated in the ratio of 1:1. Resistance to fusarium wilt is conditioned by a single highly heritable major dominant gene. The resistance can be achieved by backcross breeding