Development and application of dynamic models for environmental management of aquaculture in South East Asia

With the world’s population set to reach 9 billion by the mid 21st century food security has never been more important. Increased competition regarding land for agricultural use and over fished seas means it falls to aquaculture to meet the global demands for protein requirements. The largest supply of aquaculture products are cultivated in South East Asia where the industry has seen rapid expansion, particularly of pond production in the past 50 years. This initial expansion has come at a cost with mangrove losses and eutrophication of natural water sources resulting. The impact of these not only affects other stakeholders, including domestic users, but effects will be felt by the aquaculture industry. Indiscriminate release of effluents to the surrounding water reduces the water quality for other users and may impact on the farm discharging the water originally. Poor water quality can then result in poor growth rates and increased mortalities reducing the profitability of the farm and endangering the livelihood of the farmer. If aquaculture is to meet the global food demand it is important that current and future enterprises are developed with sustainability at the fore front. This study investigates the nutrient dynamics in pond culture in South East Asia, focussing initially on four countries outlined by the SEAT (Sustainable Ethical Aquaculture Trade) project, including Thailand, Vietnam, China and Bangladesh. Within the four countries the main species cultured for export were identified resulting in tilapia, shrimp, pangasiid catfish and prawn. Following a farmer survey designed to collect a large volume of data over a range of topics including, water management, social, economic and ethical perceptions, dynamic models were developed, using Powersim Studio 8© (Powersim, Norway), for a generic fish and shrimp ponds separately. The models draw on data from the survey combined with other literature sources to provide outputs for Total Nitrogen and Total Phosphorus in water and sediment as well as dissolved oxygen in the pond water. One of the biggest challenges facing this study was the objective selection of relevant sites for case studies to apply the models to. With such a large preselected set of sites (200 per species per country) it was important that the method be capable of handling such large datasets. Thusly it was decided that a multivariate method be used due to the removal of any pre judgement of the data relevant to the study. In order to investigate the nutrient dynamics water management data was used in the multivariate analysis to identify any similarity between the practices occurring on farms. The case studies in this project focus on Thailand and Vietnam, covering tilapia, shrimp and pangasius. Prawn farms were disregarded as, through the survey, it was discovered most production was for domestic trade. The models were adapted to each farm case study expanding the boundary from pond level to farm level, providing an output for each pond in terms of nutrients in the water and production levels and the farm as a whole for dissolved oxygen and sediment accumulation. The results of the models suggest the culture species to be taking up much of the TN added followed by the accumulation in sediments in shrimp ponds, while TP is mostly taken up by sediments. The fish case studies suggest that most of the TN is discharged to the environment followed by uptake. While Total phosphorus shows similar results to shrimp, accumulating in the sediment. The models presented in this study can be used to estimate outputs from farms of similar water management strategies and can assist in the determination of where improvements can be made to reduce the potential for eutrophication of natural water sources

What’s the Evidence for Involving Parents in Intervention for Speech Sound Disorders?

This systematic overview examines the evidence base for parent involvement in intervention for phonology-based speech sound disorders. Of the 175 identified papers, 61 reported including parents and/or home-based tasks in intervention. However, insufficient detail reported within these papers limits replication and implementation. The clinical and research implications are discussed

The efficacy of training parents to deliver multiple oppositions intervention to children with speech sound disorders

Optimal outcomes for phonology-based speech sound disorders (SSDs) can be achieved when intervention is delivered at high frequencies, such as 3 x week. However, many speech pathologists (SLPs) in Australia are unable to deliver such frequent services, with intervention typically being delivered weekly. One strategy to overcome this intensity shortfall is to engage parents in completing practice at home. The efficacy of this strategy is unclear. The results of this study showed that combined parent-SLP delivery of multiple oppositions can be used to achieve the recommended dose frequency of intervention. Child outcomes were likely influenced by multiple factors, including dosage, fidelity, and the nature of the child’s SSD. Implications for clinical practice will be discussed

An International Comparison of Intervention Practices for Children with Speech Sound Disorder

Two-hundred and seven speech-language pathologists (SLPs) from the US and 335 SLPs from Australia were surveyed about the intervention, service delivery models and intensity of intervention that they provide to children with speech sound disorder. Differences in practices, and implications for evidence-based practice and clinical outcomes, are discussed

Flexible foraging strategies in Pipistrellus pygmaeus in response to abundant but ephemeral prey

There is growing recognition that with sympathetic management, plantation forests may contain more biodiversity than previously thought. However, the extent to which they may support bat populations is contentious. Many studies have demonstrated active avoidance of coniferous plantations and attributed this to the lack of available roost sites and low invertebrate density. In contrast, other work , carried out in plantation dominated landscapes have shown that certain bat species are able to exploit these areas. However, the extent to which bats use plantations for roosting and foraging, or simply move through the plantation matrix to access more favourable sites is unclear. We radio tracked female Pipistrellus pygmaeus over two summers to establish the extent to which individual bats use Sitka Spruce plantations in southern Scotland for foraging and roosting and assess the implications for felling operations on bats. Maternity roosts identified (n=17) were in all in buildings and most were large (> 500 individuals). We found no evidence of bats roosting in mature Sitka Spruce crop trees, although several bats used roosts in old or dead beech and oak trees as an alternative to their main maternity roost. Home ranges were much larger (mean 9.6 ± 3.12 km2) than those reported from other studies ((0.6 – 1.6 km2), and it is likely that roost availability rather than food abundance constrains P. pygmaeus use of Sitka Spruce plantations. At the landscape scale, most individuals selected coniferous habitats over other habitat types, covering large distances to access plantation areas, whilst at a local scale bats used forest tracks to access water, felled stands or patches of broadleaf cover within the plantation. Sitka Spruce plantations support a high abundance of Culicoides impuctatus, the Highland midge which may act as a reliable and plentiful food source for females during lactation, an energetically expensive period. The use of felled stands for foraging by bats has implications for forest management as wind turbines, following small-scale felling operations, are increasingly being installed in plantations; wind turbines have been associated with high bat mortality in some countries. Decisions about siting wind turbines in upland plantations should consider the likelihood of increased bat activity post felling

Evaluation of Parent- and Speech-Language Pathologist–Delivered Multiple Oppositions Intervention for Children With Phonological Impairment: A Multiple-Baseline Design Study

Purpose: Evidence for the multiple oppositions intervention approach indicates it should be delivered 3 times weekly; however, this high dose frequency is not provided by many speech-language pathologists worldwide. This study investigated whether parents could be involved in delivering phonological intervention to fulfill this intensity shortfall. Method: Five children with moderate-to-severe phonological impairment aged 3;3–5;11 (years;months) and 1 of their parents participated in this study using a multiplebaseline across participants design. Participants attended one 60-min clinic-based session per week for 8 weeks, and parents completed home practice 2 times per week over this period after receiving training. Parents also attended a 60-min training session prior to commencing intervention. Results: All children showed a treatment effect to treated words. Three of the 5 children demonstrated a large effect size for generalization to nontreatment words, with 1 child demonstrating a moderate effect and 1 child demonstrating no effect. However, all children showed qualitative changes to their speech system. Three of the 5 children experienced significant changes to communicative participation. Measures of treatment fidelity indicated that parents were able to competently deliver the intervention both within the clinic and at home. Conclusions: Combined parent-and speech-language pathologist–delivered multiple oppositions intervention is effective for some children with moderate-to-severe phonological impairment. The findings indicate that parents can be trained to competently and confidently deliver phonological intervention. Further evidence is needed to identify optimal child and parent characteristics most suited to this modified service delivery approach

Interactions between phonological and motor learning : insights from an intervention study

Background: Interventions for phonological impairment, such as minimal pairs and multiple oppositions, focus on children learning the contrastive function of phonemes. Although phonological teaching procedures are typified by requests for clarification (e.g., "do you mean key or tea?"), production practice remains integral to intervention success. However, relatively little is known about optimal conditions of practice (e.g., amount, distribution, schedule) for children with phonological impairment. Purpose: To explore how the principles of motor learning might guide our understanding and application of conditions of practice for children with phonological impairment characterized by collapses of phonemic contrast. Method: Using retrospective data from five children (aged 3;3 to 5;11) involved in an 8-week intervention study of the multiple oppositions approach, four conditions of practice (practice amount, distribution, schedule, and variability) were compared with each child’s response to intervention as measured by change in percent consonants correct (PCC), generalisation to non-treatment words and phonemes, and size of phonemic collapses pre- and one-month post-intervention. Results: Two of the five children had an impressive response to intervention, with widespread generalistion and increases in PCC of more than 20% post-intervention. The remaining three children showed varied responses to intervention, with increases in PCC of less than 5%, although all added at least one or more phones to their phonetic inventory. Post-hoc analyses revealed an interaction between conditions of practice—particularly practice amount and distribution of practice—and intervention outcomes. Conclusions: Principles of motor learning, including conditions of practice, would seem to be an important consideration in intervention for phonological impairment in children. Further research is needed to better understand how the principles of motor learning might be used to understand children's responses to intervention, and how phonological interventions might be tailored to meet individual children's needs

LIM kinase inhibitors disrupt mitotic microtubule organization and impair tumor cell proliferation

The actin and microtubule cytoskeletons are critically important for cancer cell proliferation, and drugs that target microtubules are widely-used cancer therapies. However, their utility is compromised by toxicities due to dose and exposure. To overcome these issues, we characterized how inhibition of the actin and microtubule cytoskeleton regulatory LIM kinases could be used in drug combinations to increase efficacy. A previously-described LIMK inhibitor (LIMKi) induced dose-dependent microtubule alterations that resulted in significant mitotic defects, and increased the cytotoxic potency of microtubule polymerization inhibitors. By combining LIMKi with 366 compounds from the GSK Published Kinase Inhibitor Set, effective combinations were identified with kinase inhibitors including EGFR, p38 and Raf. These findings encouraged a drug discovery effort that led to development of CRT0105446 and CRT0105950, which potently block LIMK1 and LIMK2 activity in vitro, and inhibit cofilin phosphorylation and increase αTubulin acetylation in cells. CRT0105446 and CRT0105950 were screened against 656 cancer cell lines, and rhabdomyosarcoma, neuroblastoma and kidney cancer cells were identified as significantly sensitive to both LIMK inhibitors. These large-scale screens have identified effective LIMK inhibitor drug combinations and sensitive cancer types. In addition, the LIMK inhibitory compounds CRT0105446 and CRT0105950 will enable further development of LIMK-targeted cancer therapy

Validity and Reliability of the Perceived Readiness for Discharge After Birth Scale

Objective: To assess the psychometric properties of a scale measuring mothers’ perceptions of readiness for discharge after birth. Design: Psychometric analyses including construct validity using factor analysis and known groups comparisons, predictive validity, and reliability. Data were collected at discharge and 6 weeks postdischarge. Setting: Tertiary-level perinatal center in the Midwestern United States. Participants: 1,462 postpartum mothers. Intervention: None. Main Outcome Measures: Perceived Readiness for Discharge After Birth Scale scores; subscale scores for personal status and knowledge factors.\u27 Results: Exploratory and confirmatory factor analyses indicated that the scale contained two factors. Perceived Readiness for Discharge After Birth Scale scores were lower for mothers who were breast-feeding, married, primiparous, and had a short hospital stay (less than 30 hours) than for their comparison groups. The Perceived Readiness for Discharge After Birth Scale personal status factor was predictive of self-reported physical and psychosocial problems and unscheduled utilization of health services in the first 6 weeks postpartum. The knowledge factor was predictive of postdischarge telephone calls to the pediatric provider. Reliability estimates ranged from 0.83 to 0.89 for the total scale and subscales. Conclusions: The Perceived Readiness for Discharge After Birth Scale performed well in psychometric testing. Assessing mothers’perceptions of readiness for discharge is important for measuring outcomes of hospitalization and for identifying mothers at risk for postdischarge problems

Rho kinase inhibition by AT13148 blocks pancreatic ductal adenocarinoma invasion and tumor growth

The high mortality of pancreatic cancer demands that new therapeutic avenues be developed. The orally available small molecule inhibitor AT13148 potently inhibits ROCK1 and ROCK2 kinases that regulate the actomyosin cytoskeleton. We previously reported that ROCK kinase expression increases with human and mouse pancreatic cancer progression and that conditional ROCK activation accelerates mortality in a genetically modified LSL-KrasG12D; LSL-p53R172H; Pdx1-Cre; (KPC) mouse pancreatic cancer model. In this study, we show that treatment of KPC mouse and human TKCC5 patient-derived pancreatic tumor cells with AT13148, as well as the ROCK selective inhibitors Y27632 and H1152, act comparably in blocking ROCK substrate phosphorylation. AT13148, Y27632, and H1152 induced morphological changes and reduced cellular contractile force generation, motility on pliable discontinuous substrates, and three-dimensional collagen matrix invasion. AT13148 treatment reduced subcutaneous tumor growth and blocked invasion of healthy pancreatic tissue by KPC tumor cells in vivo without affecting proliferation, suggesting a role for local tissue invasion as a contributor to primary tumor growth. These results suggest that AT13148 has anti-tumor properties that may be beneficial in combination therapies or in the adjuvant setting to reduce pancreatic cancer cell invasion and slow primary tumor growth. AT13148 might also have the additional benefit of enabling tumor resection by maintaining separation between tumor and healthy tissue boundaries