Landsat analysis of the dynamics of the Chesapeake Bay plume on the continental shelf

The dynamics of the Chesapeake Bay plume have been studied by examination of 81 dates of Landsat images with color additive enhancement and single-band density slicing. The plume was interpreted from surface turbidity discontinuties as revealed in images from multispectral scanner (MSS) bands 4-7. Results show that the Chesapeake Bay plume usually frequents the Virginia coast south of the Bay mouth. Wind and tide vectors are the principal variables affecting the plume dynamics. Southwestern (compared to northern) winds spread and disperse the plume easterly over a large area. Ebb tide images (compared to flood tide images) show a more dispersed plume. Flooding waters produce high turbidity levels over the shallow northern portion of the Bay mouth

Chesapeake Bay Plume Dynamics from LANDSAT

Examination of 81 dates of Landsat images with enhancement and density slicing has shown that the Chesapeake Bay plume usually frequents the Virginia coast south of the Bay mouth. Southwestern (compared to northern) winds spread the plume easterly over a large area. Ebb tide images (compared to flood tide images) show a more dispersed plume. Flooding waters produce high turbidity levels over the shallow northern portion of the Bay mouth.https://scholarworks.wm.edu/vimsbooks/1129/thumbnail.jp

Cancer in animals : novel causes and ways of predicting biological behavior : a collection of published papers presented in application for the degree of Doctor of Science of Massey University

This Doctor of Science comprises a number of published works, listed in the References in the attached file. As such, due to copyright restriction, they are not included here but can be accessed individually from the publisher.This thesis for examination for the Doctor of Science degree consists of a collection of papers published in international peer-reviewed scientific journals. The work described was predominantly performed over the last 15 years at Massey University. While performing the research described in this thesis, I have also been involved in other non-cancer research projects. Some of the papers that describe this research are listed at the end of the reference list, but are not contained within the thesis. My interest in cancer in non-human species first began while I was working as a diagnostic pathologist at the University of Georgia. While in this position I became intrigued by the patterns of cancers that developed in different species and breeds of animals. My interest in cancer further developed into my main research focus when I returned to Massey University in 2004. During the subsequent 15 years, the majority of my research has been into the development and biological behavior of cancer in animals. This research remains important as it was pioneering in suggesting that infectious agents could be important causes of cancer in non-human species. Today, numerous studies are published every year further investigating the role of infectious agents in neoplastic diseases of animals. In addition, considering the rapid progression of veterinary oncology in the last 15 years, determining better ways to predict neoplasm behavior is currently an intense field of research in veterinary medicine

Seasat SAR test of the Virginian Sea Wave Climate Model

Coastal wave refraction imaged by the Seasat Synthetic Aperture Radar is compared to simulations produced by the Virginian Sea Wave Climate Model. Seasat SAR passes 974 at Cape Hatteras, and 974 and 1404 at Long Island, were examined using OFT and ZTS methods. Results generally confirm the validity of linear wave theory in modeling of shallow-water wave refraction --roughly half the deviations between VSWCM and SAR data for direction and wavelength are within 2 degrees and 10 meters. Convergence of wave orthogonals is found in predicted caustic regions. Available bathymetric data were adequate for the analysis. Some details in the pattern of deviations near Cape Hatteras suggested current shear and tidal effects associated with the Gulf Stream

In the dedicated pursuit of dedicated capital: restoring an indigenous investment ethic to British capitalism

Tony Blair’s landslide electoral victory on May 1 (New Labour Day?) presents the party in power with a rare, perhaps even unprecedented, opportunity to revitalise and modernise Britain’s ailing and antiquated manufacturing economy.* If it is to do so, it must remain true to its long-standing (indeed, historic) commitment to restore an indigenous investment ethic to British capitalism. In this paper we argue that this in turn requires that the party reject the very neo-liberal orthodoxies which it offered to the electorate as evidence of its competence, moderation and ‘modernisation’, which is has internalised, and which it apparently now views as circumscribing the parameters of the politically and economically possible

Perturbation of the Dimer Interface of Triosephosphate Isomerase and its Effect on Trypanosoma cruzi

Most of the enzymes of parasites have their counterpart in the host. Throughout evolution, the three-dimensional architecture of enzymes and their catalytic sites are highly conserved. Thus, identifying molecules that act exclusively on the active sites of the enzymes from parasites is a difficult task. However, it is documented that the majority of enzymes consist of various subunits, and that conservation in the interface of the subunits is lower than in the catalytic site. Indeed, we found that there are significant differences in the interface between the two subunits of triosephosphate isomerase from Homo sapiens and Trypanosoma cruzi (TcTIM), which causes Chagas disease in the American continent. In the search for agents that specifically inhibit TcTIM, we found that 2,2′-dithioaniline (DTDA) is far more effective in inactivating TcTIM than the human enzyme, and that its detrimental effect is due to perturbation of the dimer interface. Remarkably, DTDA prevented the growth of Escherichia coli cells that had TcTIM instead of their own TIM and killed T. cruzi epimastigotes in culture. Thus, this study highlights a new approach base of targeting molecular interfaces of dimers

Cross-species oncogenomics offers insight into human muscle-invasive bladder cancer

Background In humans, muscle-invasive bladder cancer (MIBC) is highly aggressive and associated with a poor prognosis. With a high mutation load and large number of altered genes, strategies to delineate key driver events are necessary. Dogs and cats develop urothelial carcinoma (UC) with histological and clinical similarities to human MIBC. Cattle that graze on bracken fern also develop UC, associated with exposure to the carcinogen ptaquiloside. These species may represent relevant animal models of spontaneous and carcinogen-induced UC that can provide insight into human MIBC. Results Whole-exome sequencing of domestic canine (n = 87) and feline (n = 23) UC, and comparative analysis with human MIBC reveals a lower mutation rate in animal cases and the absence of APOBEC mutational signatures. A convergence of driver genes (ARID1A, KDM6A, TP53, FAT1, and NRAS) is discovered, along with common focally amplified and deleted genes involved in regulation of the cell cycle and chromatin remodelling. We identify mismatch repair deficiency in a subset of canine and feline UCs with biallelic inactivation of MSH2. Bovine UC (n = 8) is distinctly different; we identify novel mutational signatures which are recapitulated in vitro in human urinary bladder UC cells treated with bracken fern extracts or purified ptaquiloside. Conclusion Canine and feline urinary bladder UC represent relevant models of MIBC in humans, and cross-species analysis can identify evolutionarily conserved driver genes. We characterize mutational signatures in bovine UC associated with bracken fern and ptaquiloside exposure, a human-linked cancer exposure. Our work demonstrates the relevance of cross-species comparative analysis in understanding both human and animal UC

Early-onset progressive retinal atrophy associated with an IQCB1 variant in African black-footed cats (Felis nigripes)

African black-footed cats (Felis nigripes) are endangered wild felids. One male and full-sibling female African black-footed cat developed vision deficits and mydriasis as early as 3 months of age. The diagnosis of early-onset progressive retinal atrophy (PRA) was supported by reduced direct and consensual pupillary light reflexes, phenotypic presence of retinal degeneration, and a non-recordable electroretinogram with negligible amplitudes in both eyes. Whole genome sequencing, conducted on two unaffected parents and one affected offspring was compared to a variant database from 51 domestic cats and a Pallas cat, revealed 50 candidate variants that segregated concordantly with the PRA phenotype. Testing in additional affected cats confirmed that cats homozygous for a 2 base pair (bp) deletion within IQ calmodulin-binding motif-containing protein-1 (IQCB1), the gene that encodes for nephrocystin-5 (NPHP5), had vision loss. The variant segregated concordantly in other related individuals within the pedigree supporting the identification of a recessively inherited early-onset feline PRA. Analysis of the black-footed cat studbook suggests additional captive cats are at risk. Genetic testing for IQCB1 and avoidance of matings between carriers should be added to the species survival plan for captive management

Precision medicine in cats:novel niemann-pick type C1 diagnosed by whole-genome sequencing

State-of-the-art health care includes genome sequencing of the patient to identify genetic variants that contribute to either the cause of their malady or variants that can be targeted to improve treatment. The goal was to introduce state-of-the-art health care to cats using genomics and a precision medicine approach. To test the feasibility of a precision medicine approach in domestic cats, a single cat that presented to the University of Missouri, Veterinary Health Center with an undiagnosed neurologic disease was whole-genome sequenced. The DNA variants from the cat were compared to the DNA variant database produced by the 99 Lives Cat Genome Sequencing Consortium. Approximately 25× genomic coverage was produced for the cat. A predicted p.H441P missense mutation was identified in NPC1, the gene causing Niemann-Pick type C1 on cat chromosome D3.47456793 caused by an adenine-to-cytosine transversion, c.1322A>C. The cat was homozygous for the variant. The variant was not identified in any other 73 domestic and 9 wild felids in the sequence database or 190 additionally genotyped cats of various breeds. The successful effort suggested precision medicine is feasible for cats and other undiagnosed cats may benefit from a genomic analysis approach. The 99 Lives DNA variant database was sufficient but would benefit from additional cat sequences. Other cats with the mutation may be identified and could be introduced as a new biomedical model for NPC1. A genetic test could eliminate the disease variant from the population