Eficacia de un desinfectante sobre Vibrio ordalii, Vibrio anguillarum, Francisella sp. y Virus de la Necrosis Pancreática Infecciosa (IPNV), patógenos de salmón del Atlántico (Salmo salar) cultivado en Chile

Indexación: ScieloRESUMEN En el presente trabajo se evaluó la eficacia in vitro del desinfectante Duplalim®, una combinación sinérgica de glutaraldehído y sales de amonio cuaternario de cuarta generación, contra 4 patógenos de peces prevalentes de la salmonicultura chilena. Los resultados muestran que todas las concentraciones ensayadas (diluciones entre 1:200 a 1:400) fueron eficaces sobre los aislados de Vibrio ordalii y Vibrio anguillarum post-30 s de exposición, detectando niveles de reducción igual a 1.8 x 106 UFC/ml. Concentraciones superiores de Duplalim® (dilución 1:50) y un tiempo de exposición no menor a 5 min. Fueron necesarios para eliminar completamente al patógeno intracelular Francisella sp. Cuando el desinfectante fue ensayado contra el Virus de la necrosis Pancreática infecciosa (IPNV), se detectó que la dilución 1:400 tiene un efecto significativo después de 2 minutos sin importar los títulos de IPNV testeados (mayor concentración evaluada 107.6 TCID50/ml). Duplalim® se evaluó en condiciones masivas contra los miembros de la familia Vibrionaceae. En comparación a los controles (sin adición desinfectante), la dilución 1:400 de Duplalim® eliminó completamente V. ordalii y V. anguillarum después de 15 minutos de tratamiento, tanto en el agua de cultivo como en la superficie de mallas usadas en el cultivo del salmón. Así, el análisis microbiológico del agua de los controles mostró concentraciones de 1.4 ± 0.3 × 106 UFC/ml, mientras en el caso de las mallas 7.6 ± 3.2 × 105 UFC/ml1. En resumen, los antecedentes obtenidos indican que el uso del desinfectante Duplalim® es efectivo contra V. ordalii, V. anguillarum y IPNV en bajas concentraciones y cortos periodos de exposición (dilución 1:400 por 15 min.), mientras que para el patógeno intracelular se requiere una concentración mayor. Palabras clave: desinfectante, patógenos de peces, salmón del atlántico. SUMMARY The efficacy of the disinfectant Duplalim®, a synergistic blend of superquats and glutaraldehyde, was analysed in vitro against 4 fish pathogens. All concentrations tested (1:200 to 1:400 dilutions) were efficacious on killing Vibrio ordalii and Vibrio anguillarum in seawater after 30 s, being the level of reduction equal to 1.8 x 106 CFU/ml. Higher concentration of Duplalim® (1:50 dilutions) and time of exposure (at least 5 min) is needed to kill completely Francisella sp, an intracellular freshwater pathogen. When Infectious Pancreatic Necrosis Virus (IPNV) was treated with 1:400 disinfectant dilution, this concentration had a significant effect after 2 minutes, regardless of the IPNV titres employed (concentration greater than 107.6 TCID50/ml). Duplalim® was tested in large scale against Vibrionaceae members. In comparison to the controls (without the disinfectant), 1:400 dilutions of Duplalim® totally killed V. ordalii and V. anguillarum in seawater as well as on the surface of the fishing net (used in the cages of cultured salmon) after 15 min. Cultivable bacteria remained constant in the buckets without the disinfectant (1.4 ± 0.3 × 106 CFU/ml), regardless of the period sampled. In the case of the adherence on the fishing net, bacteria not exposed to the disinfectant were detected at a concentration of 7.6 ± 3.2 × 105 CFU/ml. These data indicate that the use of Duplalim® against V. ordalii, V. anguillarum and IPNV is effective in low concentration and short time of exposure (15 min at a concentration of 1:400 dilutions), while the intracellular pathogen requires higher concentration. Key words: disinfectant, Chilean fish pathogens, Atlantic salmon

Assessment of the potential of MERIS near-infrared water vapour products to correct ASAR interferometric measurements

Atmospheric water vapour is a major limitation for high precision Interferometric Synthetic Aperture Radar (InSAR) applications due to its significant impact on microwave signals. We propose a statistical criterion to test whether an independent water vapour product can reduce water vapour effects on InSAR interferograms, and assess the potential of the Medium Resolution Imaging Spectrometer (MERIS) near-infrared water vapour products for correcting Advanced SAR (ASAR) data. Spatio-temporal comparisons show c. 1.1mm agreement between MERIS and GPS/radiosonde water vapour products in terms of standard deviations. One major limitation with the use of MERIS water vapour products is the frequency of cloud free conditions. Our analysis indicates that in spite of the low global cloud free conditions (~25%), the frequency can be much higher for certain areas such as Eastern Tibet (~38%) and Southern California (~48%). This suggests that MERIS water vapour products show potential for correcting ASAR interferometric measurements in certain regions

Stratigraphical evidence of Elysium sea ice from HiRise images

Abstract not available

Morphological evidence for a sea-ice origin for Elysium Planitia platy terrain

Abstract not available

MISR stereoscopic image matchers: techniques and results

The Multi-angle Imaging SpectroRadiometer (MISR) instrument, launched in December 1999 on the NASA EOS Terra satellite, produces images in the red band at 275-m resolution, over a swath width of 360 km, for the nine camera angles 70.5/spl deg/, 60/spl deg/, 45.6/spl deg/, and 26.1/spl deg/ forward, nadir, and 26.1/spl deg/, 45.6/spl deg/, 60/spl deg/, and 70.5/spl deg/ aft. A set of accurate and fast algorithms was developed for automated stereo matching of cloud features to obtain cloud-top height and motion over the nominal six-year lifetime of the mission. Accuracy and speed requirements necessitated the use of a combination of area-based and feature-based stereo-matchers with only pixel-level acuity. Feature-based techniques are used for cloud motion retrieval with the off-nadir MISR camera views, and the motion is then used to provide a correction to the disparities used to measure cloud-top heights which are derived from the innermost three cameras. Intercomparison with a previously developed "superstereo" matcher shows that the results are very comparable in accuracy with much greater coverage and at ten times the speed. Intercomparison of feature-based and area-based techniques shows that the feature-based techniques are comparable in accuracy at a factor of eight times the speed. An assessment of the accuracy of the area-based matcher for cloud-free scenes demonstrates the accuracy and completeness of the stereo-matcher. This trade-off has resulted in the loss of a reliable quality metric to predict accuracy and a slightly high blunder rate. Examples are shown of the application of the MISR stereo-matchers on several difficult scenes which demonstrate the efficacy of the matching approach

Sedimentary rocks in Bequerel crater: origin as polar layered deposits during high obliquity

Abstract not available

Hot subdwarf stars in close-up view. I. Rotational properties of subdwarf B stars in close binary systems and nature of their unseen companions

Original article can be found at: http://www.aanda.org/ Copyright The European Southern Observatory (ESO)The origin of hot subdwarf B stars (sdBs) is still unclear. About half of the known sdBs are in close binary systems for which common envelope ejection is the most likely formation channel. Little is known about this dynamic phase of binary evolution. Since most of the known sdB systems are single-lined spectroscopic binaries, it is difficult to derive masses and unravel the companions' nature, which is the aim of this paper. Due to the tidal influence of the companion in close binary systems, the rotation of the primary becomes synchronised to its orbital motion. In this case it is possible to constrain the mass of the companion, if the primary mass, its projected rotational velocity as well as its surface gravity are known. For the first time we measured the projected rotational velocities of a large sdB binary sample from high resolution spectra. We analysed a sample of 51 sdB stars in close binaries, 40 of which have known orbital parameters comprising half of all such systems known today. Synchronisation in sdB binaries is discussed both from the theoretical and the observational point of view. The masses and the nature of the unseen companions could be constrained in 31 cases. We found orbital synchronisation most likely to be established in binaries with orbital periods shorter than . Only in five cases it was impossible to decide whether the sdB's companion is a white dwarf or an M dwarf. The companions to seven sdBs could be clearly identified as late M stars. One binary may have a brown dwarf companion. The unseen companions of nine sdBs are white dwarfs with typical masses. The mass of one white dwarf companion is very low. In eight cases (including the well known system KPD1930+2752) the companion mass exceeds , four of which even exceed the Chandrasekhar limit indicating that they may be neutron stars. Even stellar mass black holes are possible for the most massive companions. The distribution of the inclinations of the systems with low mass companions appears to be consistent with expectations, whereas a lack of high inclinations becomes obvious for the massive systems. We show that the formation of such systems can be explained with common envelope evolution and present an appropriate formation channel including two phases of unstable mass transfer and one supernova explosion. The sample also contains a candidate post-RGB star, which rotates fast despite its long orbital period. The post-RGB stars are expected to spin-up caused by their ongoing contraction. The age of the sdB is another important factor. If the EHB star is too young, the synchronisation process might not be finished yet. Estimating the ages of the target stars from their positions on the EHB band, we found PG 2345+318, which is known not to be synchronised, to lie near the zero-age extreme horizontal branch as are the massive candidates PG 1232-136, PG 1432+159 and PG 1101+249. These star may possibly be too young to have reached synchronisation. The derived large fraction of putative massive sdB binary systems in low inclination orbits is inconsistent with theoretical predictions. Even if we dismiss three candidates because they may be too young and assume that the other sdB primaries are of low mass, PG 1743+477 and, in particular, HE 0532-4503 remain as candidates whose companions may have masses close to or above the Chandrasekhar limit. X-ray observations and accurate photometry are suggested to clarify their nature. As high inclination systems must also exist, an appropriate survey has already been launched to find such binaries.Peer reviewe

A 3-D Multilateration: A Precision Geodetic Measurement System

A system was designed with the capability of determining 1-cm accuracy station positions in three dimensions using pulsed laser earth satellite tracking stations coupled with strictly geometric data reduction. With this high accuracy, several crucial geodetic applications become possible, including earthquake hazards assessment, precision surveying, plate tectonics, and orbital determination

A 'p-n' diode with hole and electron-doped lanthanum manganite

The hole-doped manganite La0.7Ca0.3MnO3 and the electron-doped manganite La0.7Ce0.3MnO3 undergo an insulator to metal transition at around 250 K, above which both behave as a polaronic semiconductor. We have successfully fabricated an epitaxial trilayer (La0.7Ca0.3MnO3/SrTiO3/La0.7Ce0.3MnO3), where SrTiO3 is an insulator. At room temperature, i.e. in the semiconducting regime, it exhibits asymmetric current-voltage (I-V) characteristics akin to a p-n diode. The observed asymmetry in the I-V characteristics disappears at low temperatures where both the manganite layers are metallic. To the best of our knowledge, this is the first report of such a p-n diode, using the polaronic semiconducting regime of doped manganites.Comment: PostScript text and 2 figures, to be published in Appl. Phys. Lett

Chemical analysis and aqueous solution properties of Charged Amphiphilic Block Copolymers PBA-b-PAA synthesized by MADIX

We have linked the structural and dynamic properties in aqueous solution of amphiphilic charged diblock copolymers poly(butyl acrylate)-b-poly(acrylic acid), PBA-b-PAA, synthesized by controlled radical polymerization, with the physico-chemical characteristics of the samples. Despite product imperfections, the samples self-assemble in melt and aqueous solutions as predicted by monodisperse microphase separation theory. However, the PBA core are abnormally large; the swelling of PBA cores is not due to AA (the Flory parameter chiPBA/PAA, determined at 0.25, means strong segregation), but to h-PBA homopolymers (content determined by Liquid Chromatography at the Point of Exclusion and Adsorption Transition LC-PEAT). Beside the dominant population of micelles detected by scattering experiments, capillary electrophoresis CE analysis permitted detection of two other populations, one of h-PAA, and the other of free PBA-b-PAA chains, that have very short PBA blocks and never self-assemble. Despite the presence of these free unimers, the self-assembly in solution was found out of equilibrium: the aggregation state is history dependant and no unimer exchange between micelles occurs over months (time-evolution SANS). The high PBA/water interfacial tension, measured at 20 mN/m, prohibits unimer exchange between micelles. PBA-b-PAA solution systems are neither at thermal equilibrium nor completely frozen systems: internal fractionation of individual aggregates can occur.Comment: 32 pages, 16 figures and 4 tables submitted to Journal of Interface and Colloidal Scienc