527 research outputs found

    Bell Measurements and Observables

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    A general matrix approach to study entangled states is presented, based on operator completeness relations. Bases of unitary operators are considered, with focus on irreducible representations of groups. Bell measurements for teleportation are considered, and robustness of teleportation to various kinds of non idealities is shown.Comment: 11 pages. Elsart styl

    (Pre)analytical considerations concerning the analysis of synovial calprotectin

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    Objectives: Several studies have demonstrated that synovial calprotectin is a highly accurate biomarker in diagnosing periprosthetic joint infections (PJI). Assuring reliability is of great importance and coincides with adequate preanalytical handling. This study focuses on potentially interfering factors.Methods: To assess the stability of synovial calprotectin, the effect of time, storage temperature, EDTA, freeze-thaw cycles, viscosity, and blood and lipid contamination was investigated. In the blood and lipid contamination experiments, hemolyzed and non-hemolyzed blood, homogenized adipose tissue, intralipid and chylomicrons were added. The effect of viscosity was investigated using freeze-thaw cycles, enzymatic pretreatment and sonification.Results: No effect on synovial calprotectin levels was observed in synovial samples kept at room temperature compared to samples kept at 4°C for up to seven days of storage. Freeze-thaw cycles did not result in significantly different calprotectin levels, although samples without EDTA resulted in higher recoveries after 1 and 2 freeze-thaw cycles. Blood and lipid contamination did not interfere with accurate synovial calprotectin analysis. Sample pretreatment to reduce sample viscosity by pretreating samples with DNAse and/or hyaluronidase did not influence calprotectin analysis. Sonification, however, resulted in increased calprotectin values.Conclusions: Synovial calprotectin is a stable biomarker and its analysis is not easily influenced by potential interfering factors.</p

    (Pre)analytical considerations concerning the analysis of synovial calprotectin

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    Objectives: Several studies have demonstrated that synovial calprotectin is a highly accurate biomarker in diagnosing periprosthetic joint infections (PJI). Assuring reliability is of great importance and coincides with adequate preanalytical handling. This study focuses on potentially interfering factors.Methods: To assess the stability of synovial calprotectin, the effect of time, storage temperature, EDTA, freeze-thaw cycles, viscosity, and blood and lipid contamination was investigated. In the blood and lipid contamination experiments, hemolyzed and non-hemolyzed blood, homogenized adipose tissue, intralipid and chylomicrons were added. The effect of viscosity was investigated using freeze-thaw cycles, enzymatic pretreatment and sonification.Results: No effect on synovial calprotectin levels was observed in synovial samples kept at room temperature compared to samples kept at 4°C for up to seven days of storage. Freeze-thaw cycles did not result in significantly different calprotectin levels, although samples without EDTA resulted in higher recoveries after 1 and 2 freeze-thaw cycles. Blood and lipid contamination did not interfere with accurate synovial calprotectin analysis. Sample pretreatment to reduce sample viscosity by pretreating samples with DNAse and/or hyaluronidase did not influence calprotectin analysis. Sonification, however, resulted in increased calprotectin values.Conclusions: Synovial calprotectin is a stable biomarker and its analysis is not easily influenced by potential interfering factors.</p

    (Pre)analytical considerations concerning the analysis of synovial calprotectin

    Get PDF
    Objectives: Several studies have demonstrated that synovial calprotectin is a highly accurate biomarker in diagnosing periprosthetic joint infections (PJI). Assuring reliability is of great importance and coincides with adequate preanalytical handling. This study focuses on potentially interfering factors.Methods: To assess the stability of synovial calprotectin, the effect of time, storage temperature, EDTA, freeze-thaw cycles, viscosity, and blood and lipid contamination was investigated. In the blood and lipid contamination experiments, hemolyzed and non-hemolyzed blood, homogenized adipose tissue, intralipid and chylomicrons were added. The effect of viscosity was investigated using freeze-thaw cycles, enzymatic pretreatment and sonification.Results: No effect on synovial calprotectin levels was observed in synovial samples kept at room temperature compared to samples kept at 4°C for up to seven days of storage. Freeze-thaw cycles did not result in significantly different calprotectin levels, although samples without EDTA resulted in higher recoveries after 1 and 2 freeze-thaw cycles. Blood and lipid contamination did not interfere with accurate synovial calprotectin analysis. Sample pretreatment to reduce sample viscosity by pretreating samples with DNAse and/or hyaluronidase did not influence calprotectin analysis. Sonification, however, resulted in increased calprotectin values.Conclusions: Synovial calprotectin is a stable biomarker and its analysis is not easily influenced by potential interfering factors.</p

    ac Josephson effect in the resonant tunneling through mesoscopic superconducting junctions

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    We investigate ac Josephson effect in the resonant tunneling through mesoscopic superconducting junctions. In the presence of microwave irradiation, we show that the trajectory of multiple Andreev reflections can be closed by emitting or absorbing photons. Consequently, photon-assisted Andreev states are formed and play the role of carrying supercurrent. On the Shapiro steps, dc component appears when the resonant level is near a series of positions with spacing of half of the microwave frequency. Analytical result is derived in the limit of infinite superconducting gap, based on which new features of ac Josephson effect are revealed.Comment: 11 pages, 3 figure

    Anomalous diffusion and the first passage time problem

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    We study the distribution of first passage time (FPT) in Levy type of anomalous diffusion. Using recently formulated fractional Fokker-Planck equation we obtain three results. (1) We derive an explicit expression for the FPT distribution in terms of Fox or H-functions when the diffusion has zero drift. (2) For the nonzero drift case we obtain an analytical expression for the Laplace transform of the FPT distribution. (3) We express the FPT distribution in terms of a power series for the case of two absorbing barriers. The known results for ordinary diffusion (Brownian motion) are obtained as special cases of our more general results.Comment: 25 pages, 4 figure

    Cercospora zeina from maize in South Africa exhibits high genetic diversity and lack of regional population differentiation

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    Muller, M.F., Barnes, I., Kunene, N.T., Crampton, B.G., Bluhm, B.H., Phillips, S., Olivier, N.A. and Berger, D.K. 2016. Cercospora zeina from maize in South Africa exhibits high genetic diversity and lack of regional population differentiation. Phytopathology 106 ; 1194-1205. South Africa is one of the leading maize-producing countries in sub-Saharan Africa. Since the 1980s, Cercospora zeina, a causal agent of gray leaf spot (GLS) of maize, has become endemic in South Africa, and is responsible for substantial yield reductions. To assess genetic diversity and population structure of C. zeina in South Africa, 369 isolates were collected from commercial maize farms in three provinces (KwaZulu-Natal, Mpumalanga and North West). These isolates were evaluated with fourteen microsatellite markers and species-specific mating type markers that were designed from draft genome sequences of C. zeina isolates from Africa (CMW 25467) and USA (USPA-4). Sixty alleles were identified across 14 loci, and gene diversity values within each province ranged from 0.18 to 0.35. High levels of gene flow were observed (Nm = 5.51), and in a few cases, identical multilocus haplotypes were found in different provinces. Overall, 242 unique multilocus haplotypes were identified with a low clonal fraction of 34%. No distinct population clusters were identified using STRUCTURE, Principle Co-ordinate analysis or Weir’s theta θ statistic. The lack of population differentiation was supported with AMOVA analyses which indicated that only 2% of the variation was attributed to variability between populations from each province. Mating-type ratios of MAT1-1 and MAT1-2 idiomorphs from 335 isolates were not significantly different from a 1:1 ratio in all provinces, which provided evidence for sexual reproduction. The draft genome of C. zeina CMW 25467 exhibited a complete genomic copy of the MAT1-1 idiomorph as well as exonic fragments of MAT genes from both idiomorphs. The high level of gene diversity, shared haplotypes at different geographical locations within South Africa, and presence of both MAT idiomorphs at all sites indicates widespread dispersal of C. zeina between maize fields in the country as well as evidence for sexual recombination. The outcomes of this genome-enabled study are important for disease management since the high diversity has implications for dispersal of fungicide resistance should it emerge and the need for diversified resistance breeding.This work is based on research supported by the National Research Foundation (NRF) of South Africa (Grants #73650, #RTF14012762416) and the Genomics Research Institute at the University of Pretoria (UP), South Africa, and the National Science Foundation, USA (Grant # 0920287 to BHB). DKB was supported by a USDA Norman E Borlaug International Agricultural Science and Technology Fellowship at the University of Arkansas, USA. Opinions expressed and conclusions arrived at, are those of the authors and are not necessarily to be attributed to NRF, UP or NSF. We acknowledge PANNAR SEED Pty Ltd, the KwaZulu-Natal Department of Agriculture, Baynesfield Estate and local farmers for access to collection sites. We thank Phillip San Miguel and the Purdue Genomics Core Facility, Purdue University, USA for genome sequencing, H.Kim for assistance with microsatellite marker design, M.Greve for drafting Fig. 1, and the University of Pretoria sequencing facility for the use of the ABI3500xl genetic analyser, supported by the NRF RISP equipment grant (# 78566).http://apsjournals.apsnet.org/loi/phytohb2016GeneticsMicrobiology and Plant PathologyPlant Scienc

    Energy Flow in the Hadronic Final State of Diffractive and Non-Diffractive Deep-Inelastic Scattering at HERA

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    An investigation of the hadronic final state in diffractive and non--diffractive deep--inelastic electron--proton scattering at HERA is presented, where diffractive data are selected experimentally by demanding a large gap in pseudo --rapidity around the proton remnant direction. The transverse energy flow in the hadronic final state is evaluated using a set of estimators which quantify topological properties. Using available Monte Carlo QCD calculations, it is demonstrated that the final state in diffractive DIS exhibits the features expected if the interaction is interpreted as the scattering of an electron off a current quark with associated effects of perturbative QCD. A model in which deep--inelastic diffraction is taken to be the exchange of a pomeron with partonic structure is found to reproduce the measurements well. Models for deep--inelastic epep scattering, in which a sizeable diffractive contribution is present because of non--perturbative effects in the production of the hadronic final state, reproduce the general tendencies of the data but in all give a worse description.Comment: 22 pages, latex, 6 Figures appended as uuencoded fil
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