Variation in the quality of meat from Irish steers at the time of slaughter.

End of Project ReportThere is no information on the variation in quality, in particular tenderness, that exists in Irish Beef nor is there information on the variation that would remain if optimum practices were imposed at all stages of the beef production chain. Evaluation of the success of measures to improve beef consistency requires information on existing variation and the minimum variation achievable.The objectives of this project were (i) to establish the variation that exists in the quality of meat from Irish cattle, (ii) to quantify the minimum variation in meat quality that can be achieved in a practical beef production system, (iii) to determine the effects and mechanisms of additional sources of variation. The conclusions from this project are: • The M. longissimus dorsi (loin) was found to be more variable than the M. semimembranosus (topside) for most quality attributes examined (tenderness, sarcomere length and pH). The scale of variation within the loin was similar to that reported by the other research groups within the EU and US. Heifers were more variable than steers for most attributes, while there was no consistent classification effect on the variability of meat quality attributes. • Tenderness was equally variable in meat from genetically similar steers, managed similarly, compared to commercial steers randomly selected from a factory lairage but matched for weight and grade.This was likely a result of both groups being crossbred beef cattle of similar age, fat score, carcass weight and managed identically post-mortem. However, variation in tenderness of both groups was less than that observed in a survey of commercial throughput (experiment 1). This decrease is attributed to better pre-and-post-slaughter handling practices. • The data suggest that selection of sires (within a breed) with better than average conformation has no deleterious effect on the eating quality of beef of their progeny.A more comprehensive comparison of sires within a breed and between breeds is required to confirm the generality of this conclusion. • In a comparison of genotypes, gender and slaughter weights, there was no evidence that variation around the mean value for tenderness differed between breeds or liveweights after 14 days ageing. Bulls were more variable than steers for some quality traits but the variation in tenderness was similar for bulls and steers after 14 days ageing. • While optimising the management of animals during the pre and post-slaughter period reduced variation in tenderness, some residual variation remained. A large percentage of the residual variation in tenderness (Warner Bratzler shear force) after 2 and 7 days post-mortem was explained by proteolysis (breakdown of myofibrillar proteins).Variation in tenderness (Warner Bratzler shear force) after 2 days post-mortem was largely explained by phosphates (energy) and proteolysis, while sensory tenderness was largely explained by phosphates and glycolytic potential. • Further work is required to reduce residual variation in Irish beef and to determine the causes of this variation

RR Lyrae mid-infrared Period-Luminosity-Metallicity and Period-Wesenheit-Metallicity relations based on Gaia DR3 parallaxes

We present new empirical infrared Period-Luminosity-Metallicity (PLZ) and Period-Wesenheit-Metallicity (PWZ) relations for RR Lyrae based on the latest Gaia EDR3 parallaxes. The relations are provided in the WISE $W1$ and $W2$ bands, as well as in the $W(W1, V - W1)$ and $W(W2, V - W2)$ Wesenheit magnitudes. The relations are calibrated using a very large sample of Galactic halo field RR Lyrae stars with homogeneous spectroscopic [Fe/H] abundances (over 1,000 stars in the $W1$ band), covering a broad range of metallicities ($-2.5 \lesssim \textrm{[Fe/H]} \lesssim 0.0$). We test the performance of our PLZ and PWZ relations by determining the distance moduli of both galactic and extragalactic stellar associations: the Sculptor dwarf spheroidal galaxy in the Local Group (finding $\bar{\mu}_{0}=19.47 \pm 0.06$), the Galactic globular clusters M4 ($\bar{\mu}_{0}=11.16 \pm 0.05$) and the Reticulum globular cluster in the Large Magellanic Cloud ($\bar{\mu}_{0}=18.23 \pm 0.06$). The distance moduli determined through all our relations are internally self-consistent (within $\lesssim$ 0.05 mag) but are systematically smaller (by $\sim$ 2-3$\sigma$) than previous literature measurements taken from a variety of methods/anchors. However, a comparison with similar recent RR Lyrae empirical relations anchored with EDR3 likewise shows to varying extents a systematically smaller distance modulus for PLZ/PWZ RR Lyrae relations.Comment: Accepted by ApJ, 14 pages, 5 Figures, 2 Table

Optimization of textural and technological parameters using response surface methodology for the development of beef products for older consumers

Red meat has a tougher texture compared with many other food products, therefore consumption is often reduced among older adults. Acidic treatments had a positive effect on WBSF values (reduced the WBSF values from 23.35 N for control to 14.83 N), and texture parameters and a combination with apple fiber and rice starch may improve the health profile of a meat product with benefits for consumers, particularly for older population. A novel meat product with a softer texture (apple fiber 0.15%, rice starch 0.30% and citric acid 0.16 M) was optimized and successfully validated in this study. The results obtained for the objective measurements of tenderness were confirmed by consumers' tenderness results (p < .05) moreover texture‐optimized beef samples were found to be more acceptable by older consumers compared with control. Meat processors have an important role in increasing the availability of suitable meat products for older consumers, by developing products that will meet their nutritional and textural needs. In this study, a novel meat product with a softer texture was developed, successfully validated and the product was found to be acceptable by older consumers. Meat processors could consider using rice starch, apple fiber, and citric acid as feasible alternative to traditional ingredients used for beef injection, in order to obtain a softer product, that appeal to older consumers with difficulties in mastication

On the dwarf irregular galaxy NGC 6822. I. Young, intermediate and old stellar populations

We present accurate and deep multi-band ($g,r,i$) photometry of the Local Group dwarf irregular galaxy NGC 6822. The images were collected with wide field cameras at 2m/4m- (INT,CTIO,CFHT) and 8m-class telescopes (SUBARU) covering a 2 square degrees FoV across the center of the galaxy. We performed PSF photometry of $\approx$7,000 CCD images and the final catalog includes more than 1 million objects. We developed a new approach to identify candidate field and galaxy stars, and performed a new estimate of the galaxy center by using old stellar tracers finding that it differs by 1.15 (RA) and 1.53 (DEC) arcmin from previous estimates. We also found that young (Main Sequence, Red Supergiants), intermediate (Red Clump, Asymptotic Giant Branch [AGB]) and old (Red Giant Branch [RGB]) stars display different radial distributions. Old stellar population is spherically distributed and extends to radial distances larger than previously estimated ($\sim$1 degree). The young population shows a well defined bar and a disk-like distribution, as suggested by radio measurements, that is off-center compared with old population. We discuss pros and cons of the different diagnostics adopted to identify AGB stars and develop new ones based on optical-NIR-MIR color-color diagrams (CCDs) to characterize Oxygen and Carbon (C) rich stars. We found a mean population ratio between Carbon and M-type (C/M) stars of 0.67$\pm$0.08 (optical/NIR/MIR) and we used the observed C/M ratio with empirical C/M-metallicity relations to estimate a mean iron abundance of [Fe/H]$\sim$-1.25 ($\sigma$=0.04 dex) that agrees quite well with literature estimates.Comment: Accepted for publication in ApJ, 34 pages, 22 figures, 6 table

Behavioral Profiles of Affected and Unaffected Siblings of Children with Autism: Contribution of Measures of Mother–Infant Interaction and Nonverbal Communication

We investigated whether deficits in social gaze and affect and in joint attention behaviors are evident within the first year of life among siblings of children with autism who go on to be diagnosed with autism or ASD (ASD) and siblings who are non-diagnosed (NoASD-sib) compared to low-risk controls. The ASD group did not differ from the other two groups at 6 months of age in the frequency of gaze, smiles, and vocalizations directed toward the caregiver, nor in their sensitivity to her withdrawal from interaction. However, by 12 months, infants in the ASD group exhibited lower rates of joint attention and requesting behaviors. In contrast, NoASD-sibs did not differ from comparison infants on any variables of interest at 6 and 12 months

Proteomic Candidate Biomarkers of Drug-Induced Nephrotoxicity in the Rat

Improved biomarkers of acute nephrotoxicity are coveted by the drug development industry, regulatory agencies, and clinicians. In an effort to identify such biomarkers, urinary peptide profiles of rats treated with two different nephrotoxins were investigated. 493 marker candidates were defined that showed a significant response to cis-platin comparing a cis-platin treated cohort to controls. Next, urine samples from rats that received three consecutive daily doses of 150 or 300 mg/kg gentamicin were examined. 557 potential biomarkers were initially identified; 108 of these gentamicin-response markers showed a clear temporal response to treatment. 39 of the cisplatin-response markers also displayed a clear response to gentamicin. Of the combined 147 peptides, 101 were similarly regulated by gentamicin or cis-platin and 54 could be identified by tandem mass spectrometry. Most were collagen type I and type III fragments up-regulated in response to gentamicin treatment. Based on these peptides, classification models were generated and validated in a longitudinal study. In agreement with histopathology, the observed changes in classification scores were transient, initiated after the first dose, and generally persistent over a period of 10–20 days before returning to control levels. The data support the hypothesis that gentamicin-induced renal toxicity up-regulates protease activity, resulting in an increase in several specific urinary collagen fragments. Urinary proteomic biomarkers identified here, especially those common to both nephrotoxins, may serve as a valuable tool to investigate potential new drug candidates for the risk of nephrotoxicity

Rightward hemispheric asymmetries in auditory language cortex in children with autistic disorder: an MRI investigation

Purpose: determine if language disorder in children with autistic disorder (AD) corresponds to abnormalities in hemispheric asymmetries in auditory language cortex. Methods: MRI morphometric study in children with AD (n = 50) to assess hemispheric asymmetries in auditory language cortex. A key region of interest was the planum temporale (PT), which is larger in the left hemisphere in most healthy individuals. Results: (i) Heschl’s gyrus and planum polare showed typical hemisphere asymmetry patterns; (ii) posterior Superior Temporal Gyrus (pSTG) showed significant rightward asymmetry; and (iii) PT showed a trend for rightward asymmetry that was significant when constrained to right-handed boys (n = 30). For right-handed boys, symmetry indices for pSTG were significantly positively correlated with those for PT. PT asymmetry was age dependent, with greater rightward asymmetry with age. Conclusions: results provide evidence for rightward asymmetry in auditory association areas (pSTG and PT) known to subserve language processing. Cumulatively, our data provide evidence for a differing maturational path for PT for lower functioning children with AD, with both pre- and post-natal experience likely playing a role in PT asymmetry

Premating Reproductive Barriers between Hybridising Cricket Species Differing in Their Degree of Polyandry

Understanding speciation hinges on understanding how reproductive barriers arise between incompletely isolated populations. Despite their crucial role in speciation, prezygotic barriers are relatively poorly understood and hard to predict. We use two closely related cricket species, Gryllus bimaculatus and G. campestris, to experimentally investigate premating barriers during three sequential mate choice steps. Furthermore, we experimentally show a significant difference in polyandry levels between the two species and subsequently test the hypothesis that females of the more polyandrous species, G. bimaculatus, will be less discriminating against heterospecific males and hence hybridise more readily. During close-range mating behaviour experiments, males showed relatively weak species discrimination but females discriminated very strongly. In line with our predictions, this discrimination is asymmetric, with the more polyandrous G. bimaculatus mating heterospecifically and G. campestris females never mating heterospecifically. Our study shows clear differences in the strength of reproductive isolation during the mate choice process depending on sex and species, which may have important consequences for the evolution of reproductive barriers