Genetic Diversity of Gilthead Seabream (Sparus aurata) Broodstocks as Determined by RAPD-PCR

The objective of this study was to determine the genetic diversity in gilthead seabream brood- stocks from two hatcheries on the northern Aegean Coast of Turkey by RAPD-PCR. Forty primers were tested for each broodstock. Twenty-five produced scorable RAPD bands in stock from the Seferihisar hatchery and 28 in stock from the Aliaga hatchery. Nineteen revealed pat- terns with scorable amplified primers in both broodstocks. Depending on primer, the number of bands varied 3-16, ranging in size from 438 to 2520 base pairs (bp). The average genetic simi- larity within stocks was 0.466 for the Seferihisar stock and 0.617 for the Aliaga. The average genetic similarity between the two broodstocks was 0.420, lower than the values within the broodstocks, and the genetic distance between the two broodstocks was 0.245

Phenotypic Differences between Lactococcus garvieae Isolates Obtained from Rainbow Trout Farms in Turkey

WOS: 000579759800017Lactococcus garvieae is one of the main pathogenic agents in rainbow trout farms in Turkey. Twenty-two L. garvieae isolates obtained from different regions in Turkey were evaluated phenotypically in the study. in all isolates, cream colored, bright, round and S-type colonies with smooth margins were seen in TSA medium. They were alive during native examination without movement. It was observed that morphologically all isolates were Gram (+), alpha-hemolytic (BA), oxidase and catalase negative and were reproduced under 21, 37 and 45 degrees C temperatures with 0-6.5 % NaCl salinity. As a result of the examination of biochemical properties with API Rapid ID 32 Strep test, it was observed that 2 L. garvieae isolates were different from other isolates in respect of sucrose utilization. 1 and 19 number isolates were negative for sucrose whereas other isolates gave positive results. 1 number isolate was different from other isolates based on maltose profile. While isolate 22 was maltose negative, the other isolates gave positive results. According to phenotypic differences, all isolates used in the study were classified as three different groups.Van Yuzuncu Yil UniversityYuzuncu Yil University [2015-VF-B179]This research was supported by Research Fund of the Van Yuzuncu Yil University. Project Number: 2015-VF-B179