57 research outputs found
Potential Beneficial Effects of Tulbaghia violacea William Henry Harvey (Alliaceae) on Cardiovascular System - A Review
Tulbaghia violacea William Henry Harvey (Harv. Alliaceae) is a small bulbous herb belonging to the family Alliaceae. It is used in South Africa to treat fever, colds, asthma, paralysis, and hypertension. Meanwhile, cardiovascular disease accounts for about 30 % of total global death, with most of these deaths occurring in low and middle-income countries. Furthermore, people in low-income countries are still largely dependent on plants in their surroundings for both prophylaxis and treatment of diseases, partly due to limited access to and cost of pharmaceuticals, and folkloric evidence of the potency of medicinal plants and/or local belief systems. Therefore, the present review aims to proffer possible ways by which T. violacea may improve cardiovascular outcomes. An extensive and systematic review of the literature was carried out, and relevant findings presented in this review. There is evidence that T. violacea may modulate the renin-angiotensin system, the autonomic nervous system, oxidative stress and haemostasis, with resultant protection of the cardiovascular system in both health and disease.Keywords: Tulbaghia violacea, Spontaneously hypertensive rats, Hypertension, Blood pressure, Heart rate, Renin-angiotensin aldosterone syste
Selecting sites to prove the concept of IAR4D in the Lake Kivu Pilot Learning Site
Selecting sites is an essential step in enabling the assessment of the impact of Integrated
Agricultural Research for Development (IAR4D) in the Lake Kivu Pilot Learning Site. This paper
reports on the process of identifying distinct administrative territories (sites) in which to establish
innovation platforms and to monitor similar communities that are experiencing alternative
agricultural research for development interventions. We show how the research design for the Sub-
Saharan Africa Challenge Programme (SSACP) has been modified to take into account the key
conditioning factors of the LKPLS without relinquishing robustness. A key change is the explicit
incorporation of accessibility to multiple markets. Candidate sites were stratified according to the
national political context, followed by good and poor accessibility to markets and finally according
to security considerations and agro-ecology. Randomisation was carried out at all levels, although
the need for paired counterfactual sites required the diagnosis of conditioning factors at the site
level. Potential sites were characterised in terms of existing or recent agricultural research
initiatives, as well as local factors that would have a direct effect on the success of interventions
seeking to improve productivity, ameliorate the degradation of natural resources and enhance
incomes through better links to markets. Fourteen sites were selected during the initial phase, and a
further ten sites were added one year afterwards due to the need for more innovation platforms to
test IAR4D. The site selection was successful in pairing action and counterfactual sites in terms of
the baseline socioeconomic conditions of farming households. The unavoidable proximity of action
and counterfactual sites, however, allows the possibility of spill-over effects and could reduce the
measurable impact of IAR4D
Institutional Innovations for Building Impact-oriented Agricultural Research, Knowledge and Development Institutions
The central development question in African agriculture is how to catalyze a more competitive, equitable and sustainable agricultural growth within the context of smallholder production systems, inefficient agricultural marketing, inefficient investments by private sector amidst degradation prone natural resources base (Lynam and Blackie, 1994; IAC, 2004; World bank, 2006 ). Concerted scholarly analyses of Science and Technology (S&T) strategies have given birth to Integrated Agricultural Research for Development (IAR4D) an organizing concept of the Innovation Systems Approach (ISA) as the promise holder. It is hypothesized that the generation, diffusion and application of impactful innovations critically depend on systemic integration of knowledge systems that promote communication, interaction and cooperation between agricultural research, education, extension, farmers, private sector and policy regulatory systems. This paper examines how the different institutional innovations arising from various permutations of linkages and interactions of ARD organizations (national, international advanced agricultural research centres and universities) influenced the different outcomes in addressing identified ARD problems. A multi-institutional, multi-disciplinary phased Participatory Action Research approach was used to pool knowledge to address outstanding and emerging challenges in three countries (DRC, Rwanda and Uganda) with 2, 16 and 24 years out of conflict,
respectively) of the Lake Kivu Pilot Learning Site. A landmark institutional innovation was the participatory establishment of twelve (12) Innovation Platforms as tools for pooling knowledge across the agricultural business, education, research and extension systems. The knowledge “pool” was to generate, diffuse and apply innovations to reduce transactions costs and create value chain based “win-win” situations. A number of innovations (e.g. International Public Goods-IPGs, market binding contracts, registered brands and/or certification processes, diversity, density and quality of networks/collective action, bulking centres, ICT application and depth of knowledge pools) were initiated. There were major breakthroughs which included bringing on board non-traditional private sector and policy maker partners, overcoming the predominant “farmer handout syndrome”, building consensus and addressing common interest challenge. Making markets work, bringing various stakeholders including universities to the community and vice-versa, appreciation of indigenous knowledge system, propelling collective soil and water conservation and demand/utilization of technologies hitherto on-shelf were other very significant breakthroughs. Sustainable operations of the Innovation Systems knowledge “pool” nurturing institutional learning were ensured through the availability of a “functional body”. The body undertook the social enterprise of organizing farmers and traders, facilitating/brokering ARD organization linkages by using multi-media to build social capital to overcome emergent knowledge, credit, market, technology and resource degradation challenges under different policy regulatory systems
Does Diabetes Accelerate the Progression of Aortic Stenosis through Enhanced Inflammatory Response within Aortic valves?
Diabetes predisposes to aortic stenosis (AS). We aimed to investigate if diabetes affects the expression of selected coagulation proteins and inflammatory markers in AS valves. Twenty patients with severe AS and concomitant type 2 diabetes mellitus (DM) and 40 well-matched patients without DM scheduled for valve replacement were recruited. Valvular tissue factor (TF), TF pathway inhibitor (TFPI), prothrombin, C-reactive protein (CRP) expression were evaluated by immunostaining and TF, prothrombin, and CRP transcripts were analyzed by real-time PCR. DM patients had elevated plasma CRP (9.2 [0.74–51.9] mg/l vs. 4.7 [0.59–23.14] mg/l, p = 0.009) and TF (293.06 [192.32–386.12] pg/ml vs. 140 [104.17–177.76] pg/ml, p = 0.003) compared to non-DM patients. In DM group, TF−, TFPI−, and prothrombin expression within valves was not related to demographics, body mass index, and concomitant diseases, whereas increased expression related to DM was found for CRP on both protein (2.87 [0.5–9]% vs. 0.94 [0–4]%, p = 0.01) and transcript levels (1.3 ± 0.61 vs. 0.22 ± 0.43, p = 0.009). CRP-positive areas were positively correlated with mRNA TF (r = 0.84, p = 0.036). Diabetes mellitus is associated with enhanced inflammation within AS valves, measured by CRP expression, which may contribute to faster AS progression
The contributions of muscarinic receptors and changes in plasma aldosterone levels to the anti-hypertensive effect of Tulbaghia violacea
Background: Tulbaghia violacea Harv. (Alliaceae) is used to treat various ailments, including hypertension (HTN) in
South Africa. This study aims to evaluate the contributions of muscarinic receptors and changes in plasma
aldosterone levels to its anti-hypertensive effect.
Methods: In the acute experiments, methanol leaf extracts (MLE) of T. violacea (30–120 mg/kg), muscarine (0.16
-10 μg/kg), and atropine (0.02 - 20.48 mg/kg), and/or the vehicle (dimethylsulfoxide (DMSO) and normal saline (NS))
were respectively and randomly administered intravenously in a group of spontaneously hypertensive (SHR)
weighing 300 to 350 g and aged less than 5 months. Subsequently, T. violacea (60 mg/kg) or muscarine (2.5 μg/kg)
was infused into eight SHRs, 20 min after atropine (5.12 mg/kg) pre-treatment. In the chronic (21 days) experiments,
the SHRs were randomly divided into three groups, and given the vehicle (0.2 ml/day of DMSO and NS), T. violacea
(60 mg/kg/day) and captopril (10 mg/kg/day) respectively into the peritoneum, to investigate their effects on blood
pressure (BP), heart rate (HR), and plasma aldosterone levels. Systolic BP and HR were measured using tail-cuff
plethysmography during the intervention. BP and HR were measured via a pressure transducer connecting the
femoral artery and the Powerlab at the end of each intervention in the acute experiment; and on day 22 in the
chronic experiment.
Results: In the acute experiments, T. violacea, muscarine, and atropine significantly (p < 0.05) reduced BP
dose-dependently. T. violacea and muscarine produced dose-dependent decreases in HR, while the effect of
atropine on HR varied. After atropine pre-treatment, dose-dependent increases in BP and HR were observed with
T. violacea; while the BP and HR effects of muscarine were nullified. In the chronic experiments, the T. violaceatreated
and captropril-treated groups had signicantly lower levels of aldosterone in plasma when compared to
vehicle-treated group. Compared to the vehicle-treated group, significant reduction in BP was only seen in the
captopril-treated group; while no difference in HR was observed among the groups.
Conclusion: The results obtained in this study suggest that stimulation of the muscarinic receptors and a reduction
in plasma aldosterone levels contribute to the anti-hypertesive effect of T. violacea.IS
Differential Effects of Early- and Late-Life Access to Carotenoids on Adult Immune Function and Ornamentation in Mallard Ducks (Anas platyrhynchos)
Environmental conditions early in life can affect an organism’s phenotype at adulthood, which may be tuned to perform optimally in conditions that mimic those experienced during development (Environmental Matching hypothesis), or may be generally superior when conditions during development were of higher quality (Silver Spoon hypothesis). Here, we tested these hypotheses by examining how diet during development interacted with diet during adulthood to affect adult sexually selected ornamentation and immune function in male mallard ducks (Anas platyrhynchos). Mallards have yellow, carotenoid-pigmented beaks that are used in mate choice, and the degree of beak coloration has been linked to adult immune function. Using a 2×2 factorial experimental design, we reared mallards on diets containing either low or high levels of carotenoids (nutrients that cannot be synthesized de novo) throughout the period of growth, and then provided adults with one of these two diets while simultaneously quantifying beak coloration and response to a variety of immune challenges. We found that both developmental and adult carotenoid supplementation increased circulating carotenoid levels during dietary treatment, but that birds that received low-carotenoid diets during development maintained relatively higher circulating carotenoid levels during an adult immune challenge. Individuals that received low levels of carotenoids during development had larger phytohemagglutinin (PHA)-induced cutaneous immune responses at adulthood; however, dietary treatment during development and adulthood did not affect antibody response to a novel antigen, nitric oxide production, natural antibody levels, hemolytic capacity of the plasma, or beak coloration. However, beak coloration prior to immune challenges positively predicted PHA response, and strong PHA responses were correlated with losses in carotenoid-pigmented coloration. In sum, we did not find consistent support for either the Environmental Matching or Silver Spoon hypotheses. We then describe a new hypothesis that should be tested in future studies examining developmental plasticity
Principles, design and processes of integrated agricultural research for development: experiences and lessons from LKPLS under the SSACP
With increasing recognition holding the promise of overcoming the outstanding problems faced byAfrican agriculture, IAR4D faces the danger of being ‘blurred’ by past approaches and falling short of its potential to deliver the desired impacts in diverse multi-stakeholder, biophysical, socio- economic, cultural, technological and market contexts unless its actualisation and working is clearly understood. In this paper, we present the conceptualisation and principles of and knowledge-based experiences and lessons from the implementation of the sub-Saharan Africa Challenge Programme (SSACP) in the Lake Kivu Pilot Learning Site (LKPLS). The presentation covers the formation and facilitation of IPs for the actualisation of IAR4D to evolve mechanisms for the early recognition of interlinked issues in natural resource management, productivity and value addition technologies, markets, gender and policy arrangements. These have autonomously triggered flexible, locally directed interactions to innovate options from within or outside their
environment for resolving the challenges, and have moved along a new institutional and
technological change trajectory. Emerging lessons point to the endowment of IP members with selfhelp
knowledge interactions, training in IAR4D, quality of facilitation and research to be key
determinants of the power behind of self-regulating mechanisms
The role of APOBEC3B in lung tumor evolution and targeted cancer therapy resistance
In this study, the impact of the apolipoprotein B mRNA-editing catalytic subunit-like (APOBEC) enzyme APOBEC3B (A3B) on epidermal growth factor receptor (EGFR)-driven lung cancer was assessed. A3B expression in EGFR mutant (EGFRmut) non-small-cell lung cancer (NSCLC) mouse models constrained tumorigenesis, while A3B expression in tumors treated with EGFR-targeted cancer therapy was associated with treatment resistance. Analyses of human NSCLC models treated with EGFR-targeted therapy showed upregulation of A3B and revealed therapy-induced activation of nuclear factor kappa B (NF-κB) as an inducer of A3B expression. Significantly reduced viability was observed with A3B deficiency, and A3B was required for the enrichment of APOBEC mutation signatures, in targeted therapy-treated human NSCLC preclinical models. Upregulation of A3B was confirmed in patients with NSCLC treated with EGFR-targeted therapy. This study uncovers the multifaceted roles of A3B in NSCLC and identifies A3B as a potential target for more durable responses to targeted cancer therapy.</p
Uncovering the mechanisms of research capacity development in health and social care: a realist synthesis
BACKGROUND: Research capacity development (RCD) is considered fundamental to closing the evidence-practice gap, thereby contributing to health, wealth and knowledge for practice. Numerous frameworks and models have been proposed for RCD, but there is little evidence of what works for whom and under what circumstances. There is a need to identify mechanisms by which candidate interventions or clusters of interventions might achieve RCD and contribute to societal impact, thereby proving meaningful to stakeholders. METHODS: A realist synthesis was used to develop programme theories for RCD. Structured database searches were conducted across seven databases to identify papers examining RCD in a health or social care context (1998-2013). In addition, citation searches for 10 key articles (citation pearls) were conducted across Google Scholar and Web of Science. Of 214 included articles, 116 reported on specific interventions or initiatives or their evaluation. The remaining 98 articles were discussion papers or explicitly sought to make a theoretical contribution. A core set of 36 RCD theoretical and conceptual papers were selected and analysed to generate mechanisms that map across macro contexts (individual, team, organisational, network). Data were extracted by means of 'If-Then' statements into an Excel spreadsheet. Models and frameworks were deconstructed into their original elements. RESULTS: Eight overarching programme theories were identified featuring mechanisms that were triggered across multiple contexts. Three of these fulfilled a symbolic role in signalling the importance of RCD (e.g. positive role models, signal importance, make a difference), whilst the remainder were more functional (e.g. liberate talents, release resource, exceed sum of parts, learning by doing and co-production of knowledge). Outcomes from one mechanism produced changes in context to stimulate mechanisms in other activities. The eight programme theories were validated with findings from 10 systematic reviews (2014-2017). CONCLUSIONS: This realist synthesis is the starting point for constructing an RCD framework shaped by these programme theories. Future work is required to further test and refine these findings against empirical data from intervention studies
- …