Preparasi Kitosan Dari Limbah Udang Windu dan Uji Penurunan Kolesterol pada Kelinci (Oryctolagus cuniculus) secara in vivo

The study was conducted to examined the effects of chitosan   on cholesterol rabbit under hypercholesterolemic condition. From 100 g chitin of shrimp produce 55 g chitosan. Total of twelve male rabbits were used for this research.Those rabbits were divided into three groups; (K-)) negative control group, (K+) positive (Hypercholesterolemic) group, which were high cholesterol diet for 7 weeks, P1 and P2 preventive groups , same with group B and  supplemented with 5%, 10% chitosan suspension. The results showed that groups which supplemented of chitosan suspension has shown effects on prevent the level of cholesterol

Uji Aktivitas Ekstrak Kombinasi Rimpang Kunyit (Curcuma domestica Val.) dan Kurma (Phoenix dactylifera L) sebagai Antiinflamasi Secara In Vitro

Inflamasi adalah respon perlindungan normal terhadap cedera jaringan yang disebabkan oleh trauma fisik, bahan kimia berbahaya, atau agen mikrobiologi. Lisosom dapat mensekresi enzim yang dapat menginduksi terjadinya inflamasi. Membran lisosom analog dengan membran sel darah merah manusia. Penelitian ini bertujuan untuk menentukan potensi antiinflamasi dari ekstrak kombinasi rimpang kunyit (curcuma domestica val) dan kurma (Phoenix dactylifera L) ditinjau dari kemampuannya menstabilkan membran sel darah merah. Dengan metode stabilitas membran eritrosit. Penelitian dimulai dengan pengambilan darah dan dibuat suspensi sel darah merah. Suspensi sel darah merah dibagi menjadi 3 kelompok perlakuan yaitu kontrol negatif, kontrol positif (Natrium diklofenak), dan larutan uji dengan konsentrasi 50, 75, dan 100 ppm, kemudian  di diamkan selama 30 menit dan disentrifuge. Supernatan diukur serapan dengan menggunakan spektrofotometer UV-Vis pada panjang gelombang 413 nm. Hasil penelitian diperoleh bahwa konsentrasi 100 ppm memberikan persen inhibisi paling besar yaitu 65,64%. Dari hasil yang diperoleh dapat disimpulkan bahwa semakin tinggi konsentrasi ekstrak, maka daya inflamasinya semakin bai

Analysis of Phenols and Antioxidants Infused Sappan Wood (Caesalpiniasappan L.)

Caesalpinia sappan L. is one of Caesalpiniaceae family found in Indonesia and a health drink that is often consumed by society at large in Indonesia. This study aims to theanalysis of phenol and flavonoid infuse a sappan wood (Caesalpiniasappan L.) and determine the activities of the wooden cup remedy by using DPPH (1,1-picrylhydrazyldiphenyl- 2). Sappanwood extracted using distilled solvent was conducted using infuse for 15 minutes at a temperature of 900C. Extracts infuse obtained analyzed qualitatively using the reactive dye and quantitative determination using spectrophotometry UV-Vis at a wavelength of 730 nm for analysis of phenol in total and 430 nm for analysis flavonoidtotal average level of phenols total of 554,86 mgGAE/L samples and flavonoid total of 1,0377 mgQAE/L samples. Samples wood sappan made with a concentration of 1%, 2%, 3%, 4%, 5% and 6% with the addition of DPPH by using a UV -Visible spectrophotometer. The results showed that the samples of the sappan wood 6% have the highest antioxidant activity at a wavelength of 513.1 nm

FORMULASI SARI BUAH TOMAT VARIETAS APEL (Lycopersicum esculentum Mill Pyriforme) SEBAGAI KRIM MASKER

An research has been done about formulation of tomato essence (Lycopersicum esculentum Mill) as a Cream Mask by using variation concentration of nonionic emulgator (polysorbat 20 and sorbitan monostearic) that were  formula A (concentration 4%), formula B (concentration 3%), and formula C (concentration 2%). There aim of investigation was to determine the Formulation which has an optimum stability. Stability evaluation has done by observation of organoleptic, creaming volume, dispersed globule, viscosity and yield value, and emulsion type determination before and after stress condition at 50C and 350C for 10 cycles. Observation’s result shown that there wasn’t change before and after stress condition of orgenoleptic, creaming volume, emulsion type, rheology type before and after stress condition. Statistic analyse result of parameter viscosity and yield value by means of random black design shown that formula B (concentration 3%) obtained apparent indifferent in dispersed globule, viscosity and yield value between before and after stress condition. The conclusion that cream mask has the most optimal stability is that by formula B (concentration 3%).Key Words : Formulation, Cream Mask, Tomato essenc

ANALISIS KADAR LOGAM BERAT TEMBAGA (Cu) DAN KADMIUM (Cd) PADA IKAN KAKAP (Lates calcalifer) ASAL TAKALAR SECARA SPEKTROFOTOMETRI SERAPAN ATOM

The analysis of Cuprum (Cu) and Cadmium (Cd) content had been done of Kakap fish  (Lates calcalifer)  in Tope Jawa and Satanga Island. This research aim to analyse and determine the quantity of Cuprum (Cu) and Cadnium (Cd) of Kakap fish  (Lates calcalifer)  by Atomic Absorption Spectrophotometry (AAS). This research was done by dry destruction both types of the metal, then the result of destruction dissolved in acid. Its result then analysed qualitative by specific reaktan and analysed quantitative by Atomic Absorption Spectrophotometry of wavelength 324,8 nm for the Cuprum and 228,8 nm for the Cadmium. The result of research obtained that Kakap fish in Tope Jawa and Satanga Island contain the metal of Cuprum (Cu) and Cadmium (Cd) but not exceed the boundary of rate maximum specified by DITJEN POMKey Word: Cuprum (Cu), Cadmium (Cd), Kakap fish  and AAS

Penentuan Kadar Fenolik Total Ekstrak Etanol Daun Nilam (Pogostemon Cablin Benth.) Dengan Metode Spektrofotometri Uv-vis

One of the plants that is well known in the community and is used as a traditional medicine is patchouli (Pogostemon cablin Benth.). It contains essential oils, flavonoids, saponins, tannins, glycosides, terpenoids and steroids. The alcohol content, such as patchouli alcohol and its derivatives, phenol, and a group of terpenoids in patchouli oil has antibacterial activity. The aim at this research to determine the total phenolic condition the ethanol extract from the patchouli leaves. The extract obtained was analyzed ist content using UV-Vis spectrophotometer. The measurements were done at the maximum wavelength of 662,85 nm. The result showed that the total phenolic in patchouli leave is 327,84 mgGAE /gram of extract

ANALISIS KADAR FLAVONOID DAN FENOLIK TOTAL FRAKSI ETIL ASETAT DAUN KETEPENG CINA (Senna alata (L.) Roxb) DENGAN METODE SPEKTROFOTOMETRI UV-VISIBLE

Analysis of flavonoid and phenolic content of ethyl acetate fraction have done in chinese ketepeng leaves (Senna alata (L.) Roxb) using UV-Visible spectrophotometric method. This research aimed to analyzed of flavonoids and phenolic content of ethyl acetate fraction of chinese ketepeng leaves. Chinese ketepeng leaves were extracted using ethanol 96%, and then were partitioned using a liquid-liquid partition method of ethyl acetate to obtain ethyl acetate fraction. Flavonoid and phenolic content determined using UV-VIS spectrophotometric at 415 nm wavelength for flavonoid and 730 nm for phenolic. This research results showed that the flavonoid content of ethyl acetate fraction of chinese ketepeng leaves is 2,665 mgRE/g and phenolic content of ethyl acetate fraction of chinese ketepeng leaves is 3,729 mgRE/g