Activation of Target Gene Expression in Neurons by the C. elegans RFX Transcription Factor, DAF-19

DAF-19, the only RFX transcription factor found in C. elegans, is required for the formation of neuronal sensory cilia. Four isoforms of the DAF-19 protein have been reported, and the m86 nonsense (null) mutation affecting all four isoforms has been shown to prevent cilia formation. Transcriptome analyses employing microarrays of L1 and adult stage worms were completed using RNA from daf-19(m86) worms and an isogenic wild type strain to identify additional putative DAF-19 target genes. Using transcriptional fusions with GFP, we compared the expression patterns of several potential gene targets using fluorescence confocal microscopy. Expression patterns were characterized in various genetic backgrounds in order to determine isoform-specific expression patterns. Additionally, we completed rescue experiments using cDNAs encoding specific DAF-19 isoforms in a daf-19 null genetic background. Our data indicate that several new genes are activated by DAF-19 in both ciliated and non-ciliated neurons. We are currently developing isoform-specific CRISPR/Cas9 mutants to further explore the precise mechanisms by which different DAF-19 isoforms regulate their target genes

Transcriptional studies on stress-induced meiosis in the anaerobic gut fungi Pecoramyces ruminantium strain Hef-5

This study focused on the presence of a cryptic sexual life cycle of Pecoramyces ruminantium strain Hef-5. This strain of anaerobic gut fungi (AGF) can be found in the rumen of herbivorous animals and is known to enhance plant biomass metabolism via fermentation and saccharation. In all past research, this fungus has been described as strictly asexual; however, this research has been conducted based on the behavior of the organism in its natural habitat. Many other fungal organisms have been described to exhibit cryptic sexual life cycles which may only appear while the organism is under some stress. The presence of a cryptic sexual cycle can be sensed by the expression of core meiotic genes (CMGs). The three CMGs studied are Hop2, required for homologous pairing, Mnd1, required for recombination and nuclear division, and Dmc1, required for dsDNA break repair and homologous pairing. To impart stress on the organism, samples were flushed with oxygen for increasing increments of five minutes up to one hour, two samples for each time frame. The expression of Hop2 came first at the 10-minute mark and continues to be expressed with few discrepancies through the 60 minute time frame. Dmc1 expression begins at the 20-minute mark and stays, also with few discrepancies, through until the 55-minute mark. Mnd1 is the final CMG to be expressed after 25 minutes of oxygen exposure and continues to be expressed through the 60-minute trial. These results indicate that the AGF strain Hef-5 could have a cryptic sexual cycle under these conditions. The negative control showed no expression for any of the three CMGs, supporting the hypothesis. The continuous expression of these CMGs aligns well with the time frame for meiosis I is S. cerevisiae. Future research to expand this includes a full transcriptomic study of Hef-5 as well as the study of additional CMGs involved in other phases of meiosis

Employees’ feelings about more meetings: An overt analysis and recommendations for improving meetings

Purpose – The purpose of this paper is to identify how employees feel about having more meetings and what can be done to improve employees’ feelings about their work meetings. Design/methodology/approach – Data were obtained from three samples of working adults. The first was a convenience sample recruited by undergraduate students (n = 120), the second was a stratified random sample from a metropolitan area in the southern USA (n = 126), and the third was an internet-based panel sample (n = 402). Constant comparative analysis of responses to open-ended questions was used to investigate the overarching research questions. Findings – It is found that employees enjoy meetings when they have a clear objective, and when important relevant information is shared. Consistent with conservation of resources theory, most employees are unhappy with meetings when they reduce their work-related resources (e.g. meetings constrain their time, lack structure and are unproductive). Practical implications – The data suggest that meetings appear to be both resource-draining and resource-supplying activities in the workplace. Researchers and managers should consider overtly asking about how people feel about meetings, as a means of identifying areas for future research inquiry and targets for improvement in the workplace generally. Originality/value – The paper describes one of the few studies on meetings that ask the participants overtly what their feelings are regarding their workplace meetings. Additionally, the paper illustrates the usefulness of qualitative data analysis as a means for further understanding workplace activities viewing respondents as informants. Keywords United States of America, Employees behaviour, Employees attitudes, Employees participation, Meetings, Meeting demands, Qualitative research, Thematic analysi

Risk of first and recurrent stroke in childhood cancer survivors treated with cranial and cervical radiation therapy.

PurposeTo assess, in a retrospective cohort study, rates and predictors of first and recurrent stroke in patients treated with cranial irradiation (CRT) and/or cervical irradiation at ≤18 years of age.Methods and materialsWe performed chart abstraction (n=383) and phone interviews (n=104) to measure first and recurrent stroke in 383 patients who received CRT and/or cervical radiation at a single institution between 1980 and 2009. Stroke was defined as a physician diagnosis and symptoms consistent with stroke. Incidence of first stroke was number of first strokes per person-years of observation after radiation. We used survival analysis techniques to determine cumulative incidence of first and recurrent stroke.ResultsAmong 325 subjects with sufficient follow-up data, we identified 19 first strokes (13 ischemic, 4 hemorrhagic, 2 unknown subtype) occurring at a median age of 24 years (interquartile range 17-33 years) in patients treated with CRT. Imaging was reviewed when available (n=13), and the stroke was confirmed in 12. Overall rate of first stroke was 625 (95% confidence interval [CI] 378-977) per 100,000 person-years. The cumulative incidence of first stroke was 2% (95% CI 0.01%-5.3%) at 5 years and 4% (95% CI 2.0%-8.4%) at 10 years after irradiation. With each 100-cGy increase in the radiation dose, the stroke hazard increased by 5% (hazard ratio 1.05; 95% CI 1.01-1.09; P=.02). We identified 6 recurrent strokes; 5 had available imaging that confirmed the stroke. Median time to recurrence was 15 months (interquartile range 6 months-3.2 years) after first stroke. The cumulative incidence of recurrent stroke was 38% (95% CI 17%-69%) at 5 years and 59% (95% CI 27%-92%) at 10 years after first stroke.ConclusionCranial irradiation puts childhood cancer survivors at high risk of both first and recurrent stroke. Stroke prevention strategies for these survivors are needed

High-vacuum-compatible high-power Faraday isolators for gravitational-wave interferometers

Faraday isolators play a key role in the operation of large-scale gravitational-wave detectors. Second-generation gravitational-wave interferometers such as the Advanced Laser Interferometer Gravitational-Wave Observatory (LIGO) and Advanced Virgo will use high-average-power cw lasers (up to 200 W) requiring specially designed Faraday isolators that are immune to the effects resulting from the laser beam absorption–degraded isolation ratio, thermal lensing, and thermally induced beam steering. In this paper, we present a comprehensive study of Faraday isolators designed specifically for high-performance operation in high-power gravitational-wave interferometers

A Validated Method for Identifying Unplanned Pediatric Readmission

Objective To validate the accuracy of pre-encounter hospital designation as a novel way to identify unplanned pediatric readmissions and describe the most common diagnoses for unplanned readmissions among children. Study design We examined all hospital discharges from 2 tertiary care children's hospitals excluding deaths, normal newborn discharges, transfers to other institutions, and discharges to hospice. We performed blinded medical record review on 641 randomly selected readmissions to validate the pre-encounter planned/unplanned hospital designation. We identified the most common discharge diagnoses associated with subsequent 30-day unplanned readmissions. Results Among 166 994 discharges (hospital A: n = 55 383; hospital B: n = 111 611), the 30-day unplanned readmission rate was 10.3% (hospital A) and 8.7% (hospital B). The hospital designation of “unplanned” was correct in 98% (hospital A) and 96% (hospital B) of readmissions; the designation of “planned” was correct in 86% (hospital A) and 85% (hospital B) of readmissions. The most common discharge diagnoses for which unplanned 30-day readmissions occurred were oncologic conditions (up to 38%) and nonhypertensive congestive heart failure (about 25%), across both institutions. Conclusions Unplanned readmission rates for pediatrics, using a validated, accurate, pre-encounter designation of “unplanned,” are higher than previously estimated. For some pediatric conditions, unplanned readmission rates are as high as readmission rates reported for adult conditions. Anticipating unplanned readmissions for high-frequency diagnostic groups may help focus efforts to reduce the burden of readmission for families and facilities. Using timing of hospital registration in administrative records is an accurate, widely available, real-time way to distinguish unplanned vs planned pediatric readmissions

Preserving photon qubits in an unknown quantum state with Knill dynamical decoupling: Towards an all optical quantum memory

The implementation of polarization-based quantum communication is limited by signal loss and decoherence caused by the birefringence of a single-mode fiber. We investigate the Knill dynamical decoupling scheme, implemented using half-wave plates, to minimize decoherence and show that a fidelity greater than 99% can be achieved in absence of rotation error and fidelity greater than 96% can be achieved in presence of rotation error. Such a scheme can be used to preserve any quantum state with high fidelity and has potential application for constructing all optical quantum delay line, quantum memory, and quantum repeater

Rates and characteristics of radiographically detected intracerebral cavernous malformations after cranial radiation therapy in pediatric cancer patients.

Rates and characteristics of intracerebral cavernous malformations after cranial radiation therapy remain poorly understood. Herein we report on intracerebral cavernous malformations detected on follow-up imaging in pediatric cancer patients who received cranial radiation therapy at age ≤18 years from 1980 to 2009. Through chart reviews (n = 362) and phone interviews (n = 104) of a retrospective cohort, we identified 10 patients with intracerebral cavernous malformations. The median latency time for detection of intracerebral cavernous malformations after cranial radiation therapy was 12 years (range 1-24 years) at a median age of 21.4 years (interquartile range = 15-28). The cumulative incidence was 3% (95% confidence interval 1%-8%) at 10 years post cranial radiation therapy and 14% (95% confidence interval 7%-26%) at 15 years. Three patients underwent surgical resection. Two surgical specimens were pathologically similar to sporadically occurring intracerebral cavernous malformations; one was consistent with capillary telangiectasia. Intracerebral cavernous malformations are common after cranial radiation therapy and can show a spectrum of histologic features