Tropic Transfer and Bioaccumulation of Lead Along Soil-Plant-Aphid-Ladybird Food Chain

Lead (Pb) contamination of agroecosystems is a serious issue as Pb is a persistent pollutant that is retained in soil for long, causing toxicities to organisms. This study examines biotransfer of Pb from soils treated with different concentrations of Pb through a broad bean (Vicia faba L.)–aphid (Aphis fabae Scop.)–ladybird (Coccinella transversalis Fabricius) food chain and its consequent inference for natural biological control, the ladybird. The soil was amended with Pb at the rates of 0, 25, 50, 75 and 100 mg kg−1(w/w). The amount of Pb in plant, aphid and ladybird increased in a dose-dependent manner to Pb contents in the soil. The results showed that Pb biomagnified from soil to root with transfer coefficient always \u3e 1. Biominimization of Pb occurred at the second trophic level in aphids and at the third trophic level in ladybirds as their respective transfer coefficients from shoot to aphid and aphid to ladybird were always \u3c 1. The increased elimination of Pb via aphid excreta (honeydew) and pupal exuviae in a dose-dependent manner suggests that these are possible detoxification mechanisms at two different trophic levels which control Pb bioaccumulation along the food chain. The statistically significant (p ≤ 0.05) decreases in biomass and predation rate of predatory ladybirds at 100 mg kg−1 Pb indicate that high dose of Pb in soil may have sub-lethal effects on ladybirds. Further studies at cellular and sub-cellular levels are needed to further document the potential mechanisms of achieving Pb homeostasis in ladybirds under Pb stress

Assessment of biotransfer and bioaccumulation of cadmium, lead and zinc from fly ash amended soil in mustard-aphid-beetle food chain.

The present study investigates the extent of biotransfer and bioaccumulation of cadmium (Cd), lead (Pb) and zinc (Zn) from fly ash amended soil in mustard (Brassica juncea)-aphid (Lipaphis erysimi)-beetle (Coccinella septempunctata) food chain and its subsequent implications for the beetle. The soil was amended with fly ash at the rates of 0, 5, 10, 20 and 40% (w/w). Our results showed that the uptake of Cd, Pb and Zn from soil to mustard root increased with the increase in fly ash application rates, but their root to shoot translocation was relatively restricted. Increase in chlorophyll content and dry mass of mustard plant on treatments ≥20% even at elevated accumulation of Cd (1.67mgkg(-1)), Pb (18.25mgkg(-1)) and Zn (74.45mgkg(-1) dry weight) in its shoot showed relatively higher tolerance of selected mustard cultivar to heavy metal stress. The transfer coefficient (TC(1)) of Cd from mustard shoot to aphid was always >1, indicating that Cd biomagnified in aphids at second trophic level. But, there was no biomagnification of Cd in adult beetles at third trophic level. Zinc accumulation was 2.06 to 2.40 times more in aphids than their corresponding host shoots and 1.26-1.35 times more in adult beetles than their prey (aphids) on which they fed. Lead was only metal whose TC was 0.05) biomass and predation rate of predatory beetles indicated that all levels of soil amendments with fly ash did not have any lethal or sub-lethal effects on beetles

The transfer and fate of Pb from sewage sludge amended soil in a multi-trophic food chain: a comparison with the labile elements Cd and Zn

The contamination of agroecosystems due to the presence of trace elements in commonly used agricultural materials is a serious issue. The most contaminated material is usually sewage sludge, and the sustainable use of this material within agriculture is a major concern. This study addresses a key issue in this respect, the fate of trace metals applied to soil in food chains. The work particularly addresses the transfer of Pb, which is an understudied element in this respect, and compares the transfer of Pb with two of the most labile metals, Cd and Zn. The transfer of these elements was determined from sludge-amended soils in a food chain consisting of Indian mustard (Brassica juncea), the mustard aphid (Lipaphis erysimi) and a predatory beetle (Coccinella septempunctata). The soil was amended with sludge at rates of 0, 5, 10 and 20 % (w/w). Results showed that Cd was readily transferred through the food chain until the predator trophic level. Zn was the most readily transferred element in the lower trophic levels, but transfer to aphids was effectively restricted by the plant regulating shoot concentration. Pb had the lowest level of transfer from soil to shoot and exhibited particular retention in the roots. Nevertheless, Pb concentrations were significantly increased by sludge amendment in aphids, and Pb was increasingly transferred to ladybirds as levels increased. The potential for Pb to cause secondary toxicity to organisms in higher trophic levels may have therefore been underestimated

Transfer of Copper (Cu) in the Soil–Plant–Mealybug–Ladybird Beetle Food Chain

Copper, an essential trace element, is vital for living organisms’ survival; however, despite its importance, an excessive amount of this micro-nutrient can cause harmful effects to plants and animals. The present study investigates Cu bio-transfer across multi-trophic food chain comprising soil (spiked with various concentrations of Cu), eggplant (Solanum melongena), mealybug (Ferrisia virgata), and ladybird (Nephus ryuguus). Soils were spiked with Cu at rates of 0, 100, 200, 400, and 800 mg/kg (w/w). A dose-dependent increase in the levels of Cu in plant, mealybug, and ladybird was observed in response to Cu contents of soil. Different Cu amendment caused a significant reduction in the average root and shoot dry weights per plant as well as the average body weights of F. virgata and N. ryuguus. Our findings affirmed the importance of additional research to explain the processes involved in the bio-transfer of copper across the food chain