Transaminase Enzyme and Liver Histological Profile of Mice Administered Extract of Pegagan (Centella Asiatica (L.) Urban)

The purpose of this study was to determine whether pegagan caused toxic effects on the liver. This study used completely randomized design with four treatments, ie 125, 200, 275 mg/kg BW and control, each treatment consisting of six replicates. Variables observed were the level of GPT, GOT and histological profile of liver. GPT and GOT levels were analyzed by analysis of single variant of 0.05. Histological picture of the liver include central venous dilation, inflammation, and damage to the structure of liver cells were performed by descriptive evaluation. Pegagan leaf extract did not show significant effect on GPT and GOT level in the liver of mice, whereas the histology results did not reveal any visible damage to liver cells in each dose. Administration of pegagan extract up to dose of 275 mg/kg BW was safe and would not cause damage to the liver cells

Transaminase Enzyme and Liver Histological Profile of Mice Administered Extract of Pegagan (Centella asiatica (L.) Urban)

The purpose of this study was to determine whether pegagan caused toxic effects on the liver. This study used completely randomized design with four treatments, ie 125, 200, 275 mg/kg BW and control, each treatment consisting of six replicates. Variables observed were the level of GPT, GOT and histological profile of liver. GPT and GOT levels were analyzed by analysis of single variant of 0.05. Histological picture of the liver include central venous dilation, inflammation, and damage to the structure of liver cells were performed by descriptive evaluation. Pegagan leaf extract did not show significant effect on GPT and GOT level in the liver of mice, whereas the histology results did not reveal any visible damage to liver cells in each dose. Administration of pegagan extract up to dose of 275 mg/kg BW was safe and would not cause damage to the liver cells

KEMAMPUAN ANTI MAYOR PHYSIOLOGICAL PROTEIN SUBSTRAT ECTO CYCLIC AMP INDEPENDENT SERIN/THEONIN PROTEIN KINASE (MPS ecto-CIK) DALAM MENGHAMBAT VIABILITAS SPERMATOZOA KAMBING DAN SAPI

Penelitian ini bertujuan mengetahui pengaruh dosis dan lama inkubasi anti MPS ecto-CIK dalam menghambat viabilitas spermatozoa kambing dan sapi. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan pola faktorial yang terdiri atas 2 faktor yakni dosis pengenceran (0, 5, 10, dan 15 µl) dan lama inkubasi (5, 30, 60, dan 120 menit) masing-masing 6 kali ulangan. Data yang diperoleh dianalisis dengan analisis varian dua arah, yang dilanjutkan dengan uji jarak Duncan dengan taraf signifikansi 5%. Pemberian anti MPS ecto-CIK membran spermatozoa kambing dengan konsentrasi 0, 5, 10, dan 15 µl dan lama inkubasi 5, 30, 60, dan 120 menit berpengaruh signifikan terhadap viabilitas spermatozoa kambing dan sapi (P0,05). Perlakuan anti MPS ecto-CIK pada dosis 15 µl dan lama inkubasi 120 menit terhadap spermatozoa kambing dan sapi merupakan perlakuan yang paling optimal dalam menghambat viabilitas spermatozoa kambing (45,50±11,16 dan 44,87±9,40%) dan sapi (39,08±14,40 dan 36,67±11,93%)

Phytochemicals, Antioxidant and Antifungal Properties of Acorus Calamus, Curcuma Mangga, and Allium Sativum

The purpose of this study to determine the content of phytochemicals, antioxidant and antifungal properties of the combination of Acorus calamus, Curcuma mangga, and Allium sativum. This research was descriptive qualitative, extractions were done by maceration method with ethanol with 3 different combinations (C1, C2 and C3). Phytochemical test reagent included 4 kinds of test, namely: alkaloids, flavonoids, triterpenoids, saponins and tannins. As for the antioxidant test, the method used was DPPH. The concentration used at 25, 50, 100, 200, and 400 ppm. As for the antifungal test conducted on Candida albicans with Kirby-Bauer disc methods with a concentration of 100%, followed by the MIC and MBC test with a concentration of 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78% and 0.39%. Phytochemical test results indicated the presence of the alkaloids, flavonoids and triterpenoids compounds in 3 different combinations (C1, C2 and C3). The highest antioxidant levels founded in C1 (61.75) followed by C3 (47.94) and the lowest levels founded in C2 (42.76). The antifungal test showed the inhibitory zone against C. albicans. The highest inhibitory zone was found in C1 at 5.44 ± 1.78 mm (medium category), followed by C2 at 4.08 ± 0.86 mm (medium category), and C3 at 3.05 ± 0.23 mm (medium category). As for the minimum inhibitory concentration (MIC) value got on the concentration of 0:39% and minimum fungisidal concentration (MFC) values were at a concentration of 0.78%