Genetic characterisation of a range of geographically distinct Helicoverpa armigera nucleopolyhedrovirus (HearNPV) isolates and evaluation of biological activity against South African populations of the African bollworm, Helicoverpa armigera (Hu bner) (Lepidoptera: Noctuidae)

The African bollworm, Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is a pest of economic and agricultural importance globally. It is a polyphagous pest that feeds on a wide range of host plants including economically important crops. The impact it has on agricultural systems makes its control a priority. The most common method of control is using chemical pesticides; however, continuous application of the pesticides has resulted in the development of resistance. The use of biological control has been investigated and established as an effective method of control as a standalone or part of an integrated pest management (IPM) system. The use of the baculovirus Helicoverpa armigera nucleopolyhedrovirus (HearNPV), has shown promise in the control of H. armigera. Commercial formulations based on the virus are available in many global markets. However, the identification of novel HearNPV isolates will aid in the control of H. armigera as well as provide alternative isolates that may have better virulence. Three new HearNPV isolates were purified and identified from three distinct geographical South African locations H. armigera cadavers and named HearNPV-Albany, HearNPV-KZN and HearNPV-Haygrove. The genomes of two of the HearNPV isolates, namely HearNPV-Albany and HearNPV-KZN were genetically characterised and compared to other geographically distinct HearNPV isolates. Virulence studies were performed comparing the new HearNPV isolates against established commercial HearNPV formulations, Helicovir™ and Helicovex® and other geographically distinct isolated HearNPV, HearNPV-G4 and HearNPV-SP1. Two laboratory colonies were established using H. armigera collected from South African fields in the Belmont Valley near Grahamstown labelled as Albany colony and a colony provided from Haygrove Eden farm near George labelled as Haygrove colony. Biological studies were carried out using the Albany H. armigera colony comparing the rate of development, survival and fertility on bell green peppers, cabbage leaves and on artificial diet. From the biological studies, it was recorded that development and survivorship was best on artificial diet. Regular quality control was required for the maintenance of the colony and continuous generations of healthy larvae were eventually established. Diseased cadavers with signs of baculovirus infection were collected after bioprospecting from the Kwa-Zulu Natal Province in South Africa and were labelled KZN isolate; Belmont Valley near Grahamstown and were labelled Albany isolate; and Haygrove Eden farm near George and were labelled Haygrove isolate for the study. A fourth isolate made up of a crude extract of occlusion bodies (OBs) first described by Whitlock was also analysed and labelled Whitlock isolate. Occlusion bodies were extracted, purified and morphologically identified from the KZN, Albany, Haygrove and Whitlock isolates using TEM. Genomic DNA, which was extracted from the purified OBs. Using PCR, the identity of the OBs as HearNPV was confirmed. Genomic analyses were performed on HearNPV-Albany and HearNPV-KZN through genetic characterisation and comparison with other geographically distinct HearNPV genomes to confirm novelty and establish potential genetic relationships between the isolates through evolutionary distances. Full genomic sequencing of the isolated HearNPV and comparison with other geographically distinct HearNPV isolates identified genomic differences that showed that the HearNPV isolates were novel. HearNPV-Albany and HearNPV-KZN were successfully sequenced and identified as novel isolates with unique fragment patterns and unique gene sequences through deletions or insertions when compared to other geographically distinct HearNPV. This raised the potential for differences in biological activity against H. armigera larvae when tested through biological assays. HearNPV-Whit genome assembly had low quality data which resulted in many gaps and failed assembly. The biological activity of HearNPV isolates from Spain, China, South Africa and two commercial formulations were studied against the laboratory established H. armigera South African colony. The LC50 values of the different South African HearNPV isolates were established to be between 7.7 × 101 OBs.ml-1 for the most effective and 3.2 × 102 OBs.ml-1 for the least effective. The Spanish and Chinese HearNPV isolates resulted in LC50 values of 2.0 × 102 OBs.ml-1 and 1.2 × 101 OBs.ml-1 respectively. The commercial formulations resulted in the least virulence observed with an LC50 of 5.84× 102 OBs.ml-1 and 9.0 × 102 OBs.ml-1 for Helicovex® and Helicovir™ respectively. In this study, novel South African HearNPV isolates were isolated and identified. Through characterisation and bioassays against South African H. armigera populations the HearNPV isolates were shown to have different virulence in comparison to geographically distinct isolates. From this research, there is potential for development of new H. armigera biopesticides based on the novel isolates after field trial testing

Zinc fertilization increases productivity and grain nutritional quality of cowpea (Vigna unguiculata [L.] Walp.) under integrated soil fertility management

Cowpea (Vigna unguiculata [L.] Walp.) is an important but under-studied grain legume which can potentially contribute to improved dietary zinc (Zn) intake in sub-Saharan Africa. In this study, surveys were conducted on smallholder farms in Zimbabwe during 2014/15 to determine the influence of diverse soil fertility management options on cowpea grain productivity and nutrition quality. Guided by the surveys, field experiments were conducted to investigate the influence of Zn fertilizer on the productivity and quality of cowpea under integrated soil fertility management (ISFM). Experiments were conducted on two soil-types, namely, sandy (6% clay) and red clay (57% clay) in 2014/15 and 2015/16 where cowpea was grown in rotation with staple maize (Zea mays L.) and fertilized with combinations of Zn, nitrogen (N), phosphorus (P) and two organic nutrient resources, cattle manure and woodland leaf litter. Cowpea grain yields on surveyed farms ranged from 0.3 to 0.9 t ha−1, with grain Zn concentration ranging from 23.9 to 30.1 mg kg−1. The highest grain Zn concentration was on fields where organic nutrient resources were applied in combination with mineral N and P fertilizers. Within the field experiments, mean grain yields of cowpea increased by between 12 and 18% on both soil types when Zn fertilizer was applied, from a baseline of 1.6 and 1.1 t ha−1 on red clay and sandy soils, respectively. When Zn fertilizer was co-applied with organic nutrient resources, grain Zn concentrations of cowpea reached 42.1 mg kg−1 (red clay) and 44.7 mg kg−1 (sandy) against grain Zn concentrations of 35.9 mg kg−1 and 31.1 mg kg−1 measured in cowpea grown with no Zn fertilizer on red clay and sandy soils, respectively. Agronomic biofortification of legumes is feasible and has the potential to contribute significantly towards increasing dietary Zn intake by humans. A greater increase in grain Zn of cowpea grown on sandy than red clay soils under Zn fertilization illustrates the influence of soil type on Zn uptake, which should be explored further in agronomic biofortification programs

CLIMATE CHANGE AND VARIABILITY: SMALLHOLDER FARMING COMMUNITIES IN ZIMBABWE PORTRAY A VARIED UNDERSTANDING

Increasing awareness of risks associated with climate change and variability among smallholder farmers is critical in building their capacity to develop the necessary adaptive measures. Using farmer participatory research approaches and formal questionnaire surveys, interaction has been made with >800 farmers in two distinct smallholder farming systems of Makoni and Wedza Districts in eastern Zimbabwe to determine the current level of understanding of climate change and variability, current responses to perceived changes, as well as identify sources of agro-meteorological information. The results indicated that farmers portrayed a varied understanding both within and across the study sites. While poor rainfall distribution was seen as the major indicator for climate change by over two-thirds of the respondents in both sites, more farmers in Makoni attributed delay in onset of rains, high incidences of flush floods and unpredictable \u2018wind movements\u2019 yielding cyclones to climate change. In Wedza, it was recurrent droughts, winter and summer temperature extremes, and increased pest and disease incidences for both crops and livestock that indicated climate change. Perceived changes were linked more to natural and human forces (Makoni), unknown forces as well as breakdown in cultural norms and beliefs and rise of Christianity (Wedza). Disparities between the two sites could be attributed to the inherent differences of the communities in terms to their social settings. The national extension, Agritex, was ranked first by 50-60% of the farmers as major source of weather information. Electronic media (radio and television) ranked second with 47% of farmers in Makoni and 35% in Wedza. Concerns were raised over inadequacies of such information, apparent lack of reliability, timing and frequency of dissemination that directly influenced the utilisation of the information. Common coping strategies included early planting which was the more prevalent in Makoni, while combinations of cereals and legumes were a preferred solution in Wedza. We concluded that farmers\u2019 interaction with various information sources and knowledge sharing platforms needs to be enhanced in order to deepen their understanding as well as increase their capacity to respond to pressures of climate variability and change.L\u2019am\ue9lioration des connaissances des petits fermiers sur les risques lies au changement du climat et sa variabilit\ue9 est un fait important pour l\u2019\ue9dification de leur capacit\ue9 de d\ue9velopper des mesures n\ue9cessaires d\u2019adaptation. En utilisant des m\ue9thodes de recherche participative et un questionnaire d\u2019enqu\ueate formelle, l\u2019interaction a \ue9t\ue9 faite avec plus de 800 fermiers de deux syst\ue8mes d\u2019exploitation distincts de districts de Makoni et Wedza \ue0 l\u2019Est du Zimbabwe, afin de d\ue9terminer le niveau actuel de compr\ue9hension du changement du climat et sa variabilit\ue9, les m\ue9canismes d\u2019adaptation aux changements per\ue7us, ainsi qu\u2019identifier les sources d\u2019information agro m\ue9t\ue9orologique. Les r\ue9sultats ont indiqu\ue9 que les fermiers avaient un niveau vari\ue9 de compr\ue9hension dans les deux sites d\u2019\ue9tude.Alors qu\u2019une faible distribution des pr\ue9cipitations \ue9tait consid\ue9r\ue9e comme \ue9tant l\u2019indicateur majeur du changement du climat par plus de 2/3 des r\ue9pondants dans les deux sites, la plupart des fermiers de Makoni ont affirm\ue9 que le retard dans l\u2019apparition des pluies, incidence \ue9lev\ue9e des inondations et mouvement du vent impr\ue9dictible r\ue9sultant du cyclone par le changement du climat. En Wedza, le changement du climat \ue9tait manifest\ue9 \ue0 travers les s\ue9cheresses r\ue9currentes, des temp\ue9ratures extr\ueames pendant l\u2019hiver et l\u2019\ue9t\ue9 ainsi que l\u2019accroissement d\u2019incidences de pestes et maladies affectant des cultures et le b\ue9tail. Les changements per\ue7us \ue9taient plus li\ue9s aux forces naturelles et humaines (Makoni), des forces inconnues aussi bien que la non-observance des normes culturales et les croyances et le d\ue9veloppement de la chr\ue9tient\ue9 (Wedza). Les disparit\ue9s entre les deux sites pourraient \ueatre attribu\ue9es aux diff\ue9rences inh\ue9rentes des deux communaut\ue9s en termes de leurs environnements sociaux. La vulgarisation nationale, Agritex, \ue9tait rang\ue9e la premi\ue8re par 50-60% des fermiers comme source majeur de l\u2019information sur le climat suivi du media \ue9lectronique (radio et t\ue9l\ue9vision) avec 47% des fermiers dans Makoni et 35% dans Wedza. Les probl\ue8mes \ue9voqu\ue9s \ue9taient li\ue9s au manque de consistance de l\u2019information, manque apparent de fiabilit\ue9, le temps et la fr\ue9quence de diss\ue9mination qui directement influencent l\u2019utilisation de l\u2019information. Les strat\ue9gies communes d\u2019adaptation consistaient en la plantation \ue0 temps sp\ue9cialement dans Makoni, pendant que les combinaisons des c\ue9r\ue9ales et des l\ue9gumes \ue9taient une solution pr\ue9f\ue9r\ue9e en Wedza. En conclusion, l\u2019interaction des fermiers avec des sources vari\ue9es d\u2019information et les plateformes de partage de connaissance ont besoin d\u2019\ueatre d\ue9velopp\ue9e afin d\u2019am\ue9liorer leur niveau de compr\ue9hension aussi bien renforcer leur capacit\ue9 \ue0 r\ue9pondre aux pressions du changement du climat et sa variabilit\ue9

Role of soil texture, clay mineralogy, location, and temperature in coarse wood decomposition—a mesocosm experiment

Of all the major pools of terrestrial carbon (C), the dynamics of coarse woody debris (CWD) are the least understood. In contrast to soils and living vegetation, the study of CWD has rarely relied on ex situ methods for elaborating controls on decomposition rates. In this study, we report on a mesocosm incubation experiment examining how clay amount (8%, 16%, and 24% clay), clay type (soil reconstructed with kaolinite vs. montmorillonite), wood placement (on litter layer surface, at the litter layer–soil interface, buried in the mineral soil), and laboratory incubation temperature (10°, 20°, or 30°C) control decomposition rates of highly standardized stakes and blocks of coarse aspen wood. Clay type effect was pronounced, with wood decomposing more quickly in kaolinite- than in montmorillonite-amended soils, perhaps due to a combined effect of moisture and microbial access to the substrate. Clay amount had only very limited effect on wood decomposition, which was a function of contact with the mineral soil (Surface \u3c Interface \u3c Mineral), perhaps due to greater contact with the decomposer community. Temperature effects were significant and dependent on interactions with clay type and wood placement. Effects of temperature on wood decomposition declined as the effects of soil variables increased, suggesting a hierarchy of controls on wood decomposition rates. Both water content and temperature had a strong effect on wood decomposition. Our results highlight that multiple interacting factors likely regulate wood decomposition processes. Multifactorial field experiments are needed to examine the physical, chemical, and biological factors controlling wood decompositio

Can nitrogen fertilizer management improve grain iron concentration of agro-biofortified crops in Zimbabwe?

Improving iron (Fe) concentration in staple grain crops could help reduce Fe-deficiency anaemia in communities dependent on plant-based diets. Co-application of nitrogen (N) and zinc (Zn) fertilizers has been reported to improve both yield and grain Zn concentration of crops in smallholder farming systems. This study was conducted to determine if similar effects are observed for grain Fe concentration. Field experiments were conducted in two years, in two contrasting agro-ecologies in Zimbabwe, on maize (Zea mays L.), cowpea (Vigna unguiculata [L.] Walp) and two finger millet (Eleusine coracana (L.) Gaertn.) “seed pools”. The two finger millet “seed pools” were collected during previous farmer surveys to represent “high” and “low” Fe concentrations. All plots received foliar Fe-ethylene diamine tetra-acetic acid (EDTA) fertilizer and one of seven N treatments, representing mineral or organic N sources, and combinations thereof. Higher grain yields were observed in larger N treatments. Grain Fe concentration increased according to species: maize < finger millet < cowpea but varied widely according to treatment. Significant effects of N-form on grain Fe concentration were observed in the low finger millet “seed pool”, for which mineral N fertilizer application increased grain Fe concentration to a greater extent than other N forms, but not for the other species. Whilst good soil fertility management is essential for yield and grain quality, effects on grain Fe concentration are less consistent than reported previously for Zn

Eliciting experts’ tacit models for the interpretation of soil information, an example from the evaluation of potential benefits from conservation agriculture

© 2020 The Authors We examined a procedure to elicit the tacit models underlying expert opinions on environmental factors that affect the absolute yield benefits expected from the adoption of conservation agriculture (CA) practices in southern Africa. The procedure is based on expert evaluation of the expected improvement in crop yield on adoption of CA in a particular scenario or ‘state’, a state being a specified set of soil conditions captured by a standard soil profile description from a specified agroecological zone (AEZ) of Zambia. Mixed groups of scientists including soil scientists, agronomists, agricultural economists and other environmental scientists, facilitated by experienced senior researchers, were presented with multiple subsets each of three states, and asked to rank the states in each subset with respect to expected yield improvement under CA. The groups of scientists could be divided into two sets. Each set comprised two groups, and the agreement on ranking between groups within each set was larger than would be expected if the ranking were done at random. For both sets of groups the ranking could be modelled with respect to properties of the soil, and the contrast between AEZ. The models revealed two contrasting groups of conceptual assumptions. One group broadly expected larger absolute yield improvements from conservation agriculture in settings where water is most likely to be limiting and the carbon status of the soil is poor. By contrast, the other group expected larger improvements where water was less likely to be limiting. These contrasting views are relevant to current discussions as to whether conservation agriculture, which is promoted as a ‘climate smart’ strategy for cropping, is sufficiently attractive for smallholder producers in conditions where crop production is already challenging, and whether the potential benefits in areas where water availability is not of itself a common limitation should be considered. The elicited models could be translated directly into competing hypotheses to be tested, perhaps in on-farm trials of conservation agriculture practices over contrasting soils in the different AEZ. The method, based on modelling the ranking process, could be of more general interest for the elicitation of expert opinion about complex soil, crop and environmental systems

Fertilizer management and soil type influence grain zinc and iron concentration under contrasting smallholder cropping systems in Zimbabwe

Micronutrient deficiencies remain prevalent in food systems of southern Africa, although advances in biofortification through crop breeding and agronomy provide opportunities to address these. We determined baseline soil availability of zinc (Zn) and iron (Fe) and the effects of soil type and farmer management on extractable soil Zn and Fe and subsequent concentration in cereal and legume grains under two contrasting agro-ecologies in Zimbabwe. Soil and crop surveys were conducted in Hwedza and Mutasa Districts of Zimbabwe in 2015–16 on 350 locations over different soil types. Fields with different levels of productivity (designated as “most” and “least” productive fields) were sampled using an inherited hierarchical randomized sampling design. Grain Zn and Fe concentration in maize (Zea mays), sorghum (Sorghum bicolor), finger millet (Eleusine coracana) and cowpea (Vigna unguiculata) were generally insufficient for adequate human nutrition. A Linear Mixed Effects (LME) model revealed that diethylene triamine penta-acetic acid- (DTPA) extractable soil Zn concentration and grain Zn concentration were affected primarily by field productivity level. DTPA-extractable soil Zn concentration was more than two-fold greater on the most productive fields (mean 0.8 mg kg−1) than on the least productive fields, with mean grain Zn concentration of 25.2 mg grain Zn kg−1 which was 13% greater than seen on the least productive fields. An interaction effect of field productivity level and total soil Zn concentration on DTPA-extractable soil Zn concentration suggests potential contribution of organic matter management to unlocking unavailable forms of soil Zn. DTPA-extractable soil Fe and grain Fe concentration were primarily affected by soil type and crop type, respectively. The LME modelling approach revealed additional soil geochemical covariates affected DTPA-extractable soil Zn and Fe concentration and grain Zn and Fe concentration within Districts. Future studies can therefore be powered to detect their roles at wider spatial scales for sustainable management of crop Zn and Fe nutrition

Good soil management can reduce dietary zinc deficiency in Zimbabwe

Dietary zinc (Zn) deficiency is widespread in sub-Saharan Africa (SSA) with adverse impacts on human health. Agronomic biofortification with Zn fertilizers and improved soil fertility management, using mineral and organic nutrient resources, has previously been shown to increase Zn concentration of staple grain crops, including maize. Here, we show the potential of different soil fertility management options on maize crops to reduce dietary Zn deficiency in Zimbabwe using secondary data from a set of survey and field experiments. An ex-ante approach was used, informed by published evidence from studies in three contrasting smallholder production systems in Zimbabwe. To estimate current Zn deficiency in Zimbabwe, data on dietary Zn supply from non-maize sources from the Global Expanded Nutrient Supply (GENuS) data set were linked to maize grain Zn composition observed under typical current soil fertility management scenarios. A baseline dietary Zn deficiency prevalence of 68% was estimated from a reference maize grain Zn composition value of 16.6 mg kg-1 and an estimated dietary Zn intake of 9.3 mg capita-1 day-1 from all food sources. The potential health benefits of reducing Zn deficiency using different soil fertility management scenarios were quantified within a Disability Adjusted Life Years (DALYs) framework. A scenario using optimal mineral NPK fertilizers and locally available organic nutrient resources (i.e. cattle manure and leaf litter), but without additional soil Zn fertilizer applications, is estimated to increase maize grain Zn concentration to 19.3 mg kg-1. This would reduce the estimated prevalence of dietary Zn deficiency to 55%, potentially saving 2,238 DALYs year-1. Universal adoption of optimal fertilizers, to include soil Zn applications and locally available organic leaf litter, is estimated to increase maize grain Zn concentration to 32.4 mg kg-1 and reduce dietary Zn deficiency to 16.7%, potentially saving 9,119 DALYs year-1. Potential monetized yield gains from adopting improved soil fertility management range from 49-to 158-fold larger than the potential reduction in DALYs, if the latter are monetized using standard methods. Farmers should be incentivized to adopt improved soil fertility management to improve both crop yield and quality

Resource flows, crops and soil fertility management in smallholder farming systems in semi-arid Zimbabwe

Poor soil fertility and erratic rains are major constraints to crop production in semi-arid environments. In the smallholder farming systems of sub-Saharan Africa, these constraints are manifested in frequent crop failures and endemic food insecurity. We characterized a semi-arid smallholder farming system in south-western Zimbabwe to assess crop production, nutrient use and factors that constrain productivity. The farming system was studied using resource flow mapping, farmer interviews and calculations of crop production over three cropping seasons (2002/2003, 2003/2004 and 2004/2005) to capture variability between years. Farmers were categorized into three groups: better resourced, medium resourced and poorly resourced. Better resourced farmers produced adequate grain for basic household consumption, except in the drought year (2002/2003). Poorly resourced farmers had large grain deficits, whereas the medium resourced class had smaller deficits. Better resourced and medium resourced farmers produced adequate amounts of staple cereal in two of the seasons, while poorly resourced farmers produced inadequate amounts of food in all three seasons. All farmers produced less than 300 kg/ha of legumes per season. Lack of seed was cited as the main reason for poor legume production. Better resourced farmers used animal manure (2000-5000 kg per season) and some fertilizer on their cereal crops, while the medium resourced group used less manure (1000 kg or less) and no fertilizer. The use of manure varied strongly across the years. Poorly resourced farmers used no nutrient inputs on any of their crops. All groups had negative nitrogen balances during the three cropping seasons, although the values varied strongly between seasons. Investigation of the potential strategies for developing sustainable production systems are required to address the problems of food security in the semi-arid parts of the country and the region