Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments

Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Bernardis, Aldo Ceferino. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Luna, Claudia Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Collavino, Mónica Mariana. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.In vitro regeneration of complete plants ffom nodal single bud segments of “yerba mate” {Ilex paraguariensis St. Hil.) was studied under defined nutritional and environmental conditions. Nodal segments harvested ffom actively growing shoots o f conventionally raised plants were cultured on nutrient médium with the mineral salts and vitamins o f Murashige and Skoog médium at 1/4 strength, supplemented with various concentrations o f sucrose and 6-benzyladenine (BAP). Shoot regeneration ffom explants o f both young (2 years oíd) and adult (20 years oíd) mother plants were readily achieved in the médium supplemented with 0.04 - 0.09 M sucrose with or without BAP. As many as 60 - 65% o f the nodal segments cultured formed shoots. Rooting o f regenerated shoots was observed in 50% o f the explants harvested ffom young plants, whereas 25% o f the explants rooted when the nodal explants were harvested ffom adult plants. The best rooting induction was achieved on 1/4 strength MS médium with vermiculite as the substrate and supplemented with 1 - 1.5% IBA (indolebutyric acid) and 1 - 2% PPZ (3- methyl - 1 - phenyl - 2 pyrazolin - 5 - one). Plantlets were successfully transferred to soil

Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration

The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.Fil: Surenciski, Mauro Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Flachsland, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Terada, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentin

Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration

ABSTRACT: The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucros

Resonanz-Raman-spektroskopische Untersuchung der Chromophorheterogenit t und Photokonversion in Phytochromkristallen und -losungen

Kristalle des Photorezeptors Phytochrom wurden zerstörungsfrei mithilfe der Resonanz‐Raman‐Spektroskopie untersucht (siehe Bild). Die Spektren der Chromophorbindungsdomäne (CBD) des Phytochroms aus Deinococcus radiodurans, von dem die dreidimensionale Struktur bestimmt wurde, zeigen im Kristall und in Lösung dieselbe Struktur. Im Unterschied zur CBD gehen Kristalle des Phytochroms aus Agrobacterium tumefaciens, das die CBD‐ und die PHY‐Domäne enthält, lichtinduziert in einen Meta‐Rc‐ähnlichen Zustand über.Fil: Von Stetten, David. Technishe Universitat Berlin; AlemaniaFil: Günther, Mina. Technishe Universitat Berlin; AlemaniaFil: Scheerer, Patrick. Universittsmedizin Berlin; AlemaniaFil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Mroginski, Maria Andrea. Technishe Universitat Berlin; AlemaniaFil: Krauß, Norbert. University of London; Reino UnidoFil: Lamparter, Tilman. Universitat Karlsruhe; AlemaniaFil: Zhang, Junrui. University of Wisconsin; Estados UnidosFil: Anstrom, David M.. University of Wisconsin; Estados UnidosFil: Vierstra, Richard D.. University of Wisconsin; Estados UnidosFil: Forest, Katrina T.. University of Wisconsin; Estados UnidosFil: Hildebrandt, Peter. Technishe Universitat Berlin; Alemani

Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments

In vitro regeneration of complete plants ffom nodal single bud segments of “yerba mate” {Ilex paraguariensis St. Hil.) was studied under defined nutritional and environmental conditions. Nodal segments harvested ffom actively growing shoots o f conventionally raised plants were cultured on nutrient médium with the mineral salts and vitamins o f Murashige and Skoog médium at 1/4 strength, supplemented with various concentrations o f sucrose and 6-benzyladenine (BAP). Shoot regeneration ffom explants o f both young (2 years oíd) and adult (20 years oíd) mother plants were readily achieved in the médium supplemented with 0.04 - 0.09 M sucrose with or without BAP. As many as 60 - 65% o f the nodal segments cultured formed shoots. Rooting o f regenerated shoots was observed in 50% o f the explants harvested ffom young plants, whereas 25% o f the explants rooted when the nodal explants were harvested ffom adult plants. The best rooting induction was achieved on 1/4 strength MS médium with vermiculite as the substrate and supplemented with 1 - 1.5% IBA (indolebutyric acid) and 1 - 2% PPZ (3- methyl - 1 - phenyl - 2 pyrazolin - 5 - one). Plantlets were successfully transferred to soil