Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus

Dengue disease is an increasing global health problem that threatens one-third of the world's population. Despite decades of efforts, no licensed vaccine against dengue is available. With the aim to develop an affordable vaccine that could be used in young populations living in tropical areas, we evaluated a new strategy based on the expression of a minimal dengue antigen by a vector derived from pediatric live-attenuated Schwarz measles vaccine (MV). As a proof-of-concept, we inserted into the MV vector a sequence encoding a minimal combined dengue antigen composed of the envelope domain III (EDIII) fused to the ectodomain of the membrane protein (ectoM) from DV serotype-1. Immunization of mice susceptible to MV resulted in a long-term production of DV1 serotype-specific neutralizing antibodies. The presence of ectoM was critical to the immunogenicity of inserted EDIII. The adjuvant capacity of ectoM correlated with its ability to promote the maturation of dendritic cells and the secretion of proinflammatory and antiviral cytokines and chemokines involved in adaptive immunity. The protective efficacy of this vaccine should be studied in non-human primates. A combined measles–dengue vaccine might provide a one-shot approach to immunize children against both diseases where they co-exist

Profound Depletion of HIV-1 Transcription in Patients Initiating Antiretroviral Therapy during Acute Infection

Early intervention resulted in profound depletion of PBMC expressing HIV-1 RNA. This is contrary to chronically infected patients who predominantly showed continuous UsRNA expression on cART. Thus, antiretroviral treatment initiated during the acute phase of infection prevented establishment or expansion of long-lived transcriptionally active viral cellular reservoirs in peripheral blood

Effect of drying conditions on indole glucosinolate level in broccoli

Broccoli is interesting as raw vegetable for domestic use and as well as dehydrated powder due to content of numerous biologically active compounds — phytochemicals. We examined the influence of blanching and drying air temperature and velocity on glucosinolate composition and content under conditions which are usually applied in industrial processing of broccoli. Broccoli blanching prior to drying caused a significant decrease in the glucosinolate content, which additionally decreased during the drying process. Drying at 50 °C and 60 °C with air velocity of 2.25 m s−1 was the most favourable in most of the cases

Exergy Analysis of Food Drying Processes

It is well known that micro-level system parameters may have some great impact on macro-level energy aspects, the environment, and sustainability. Of course, if one wants to approach these thermodynamically, there are two ways: energy analysis through the first law of thermodynamics and exergy analysis through the second law of thermodynamics. Exergy analysis is an essential tool to expose the impacts of a power generating device on exergy-based sustainability; sustainability is necessary to overcome current ecological, economic, and developmental problems (Dincer and Rosen, 2005). In this regard, some new exergy-based sustainability parameters for a PEM fuel cell have been developed and studied parametrically to investigate how they will help measure the level of environmental impact and sustainable development (Midilli and Dincer, 2009). These types of parameters may also be applied to other energy-related systems.TÜBİTAK Project No. 106M48