Mental Health and Wellbeing of South Sudanese-Australians

The majority of South Sudanese-Australians arrived in Australia, and other host countries outside Africa, after spending a greater part of their lives in refugee camps or conflict-affected areas. In addition, refugees are often not able to return to their home countries because the causes of their departure (wars, insecurity, hunger) continue to apply in their country of origin. The purpose of this paper is to examine some of the mental health and wellbeing issues some South Sudanese-Australians experience as a result of settlement difficulties and their earlier experience of conflict. The study looks at experiences of resettlement and settlement difficulties, and, more importantly, mental health and wellbeing issues in the wake of the aforesaid challenges. The data was collected from a qualitative method which comprised a series of semi-structured, one-on-one interviews with a total of 20 South Sudanese-Australians living in Melbourne. There were 11 males and nine females, with ages ranging from 18 to 64 years, who volunteered to participate in this study. Findings indicated that, as with many other people from refugee backgrounds, South Sudanese-Australians face a range of settlement-related challenges, and a host of post-resettlement adaptation experiences such as limited change of gender roles, language proficiency, unemployment, host society unacceptance or intolerance, constrained recreational opportunities, lack of community connectedness and overall mental health and general wellbeing issues. Experiencing conflict can be traumatic and refuge in neighbouring countries that are socio-culturally different can be challenging. In conclusion, these issues can not only be troublesome on a day-to-day basis, but also adversely affect the long-term mental health and wellbeing of refugees

PERCEIVED CHALLENGES OF ICT AS AN EXAMINABLE CURRICULUM SUBJECT IN RURAL SECONDARY SCHOOLS: VOICES OF TEACHERS AND LEARNERS IN SOUTHERN ZAMBIA

The study interrogated teachers’ and pupils’ perceptions of Information Communication Technology (ICT) as an examinable curriculum subject in secondary schools of Mazabuka district in Southern Zambia. In 2013 Ministry of Education (MoE) reviewed the National School Curriculum introducing ICT as an examinable subject at the junior secondary school level. This development was, however, received with mixed feelings among different stakeholders across the nation. The study employed Davis’ (1989) Technology Acceptance Model (TAM) as a theoretical framework to establish the teachers’ and pupils’ perceptions of ICT as an examinable curriculum subject. A descriptive research design was used to collect qualitative data from a sample size of 36 participants which included school administrators, ICT teachers, and pupils. Purposive and quota sampling was applied to select the participants. Data were collected using unstructured questionnaires, interviews, and focus group discussions. The data were analysed thematically by categorization of major and sub-themes that emerged from the study. The study showed that both teachers and pupils had positive perceptions towards ICT as an examinable curriculum subject. The majority of the participants strongly felt that the current times demanded for ICT knowledge in order for learners to fit well in the world of technology. The study also identified challenges faced in the teaching and learning of ICT such as; a lack of trained teachers, inadequate computer hardware and software, and irregular power supply. Various measures were employed in mitigating the challenges such as community engagement, voluntary teaching, class splitting, use of alternative sources of power, and explorations of online resources. The study recommended that the Ministry of Education should revert to its earlier policy position where all schools were to offer ICT as a compulsory examinable subject at the junior secondary school level. The government also should consider the reduction of taxes and tariffs on end-user consumer ICT products such as desktops computers, iPads, projectors, and others.  Article visualizations

SURFIN4.1, a schizont-merozoite associated protein in the SURFIN family of Plasmodium falciparum

<p>Abstract</p> <p>Background</p> <p>In its effort to survive the human immune system, <it>Plasmodium falciparum </it>uses several parasite-derived antigens most of which are expressed at the surface of the parasitized red blood cells (pRBCs). Recently SURFINs, a new family of antigens encoded by the <it>surf </it>multi-gene family, has been reported. One member of the family, SURFIN_4.2, was found present both at the pRBC-surface and at the merozoite apex.</p> <p>Methods</p> <p>The presence of a second SURFIN member, SURFIN_4.1(PFD0100c, PFD0105c) is reported here. Bioinformatic tools were used to study the structure of the <it>surf</it>_4.1gene. To investigate the expression of <it>surf </it>genes PCR and real-time quantitative PCR (Rt-QPCR) were employed and Northern and Western blots were used to confirm the size of the <it>surf</it>_4.1gene and the SURFIN_4.1protein respectively. Localization of SURFIN_4.1was determined using immunofluorescence assays.</p> <p>Results</p> <p>The <it>surf</it>_4.1gene was found present in one copy by Rt-QPCR in some parasites (3D7AH1, 3D7S8, 7G8) whereas six copies of the gene were identified in FCR3 and FCR3S1.2. <it>surf</it>_4.1was found transcribed in the late asexual stages of the parasite beginning ≈32 hours post invasion and throughout the schizont stages with the level of transcription peaking at late schizogony. The levels of transcript correlated with the number of gene copies in FCR3 and 3D7S8. <it>surf</it>_4.1was found to encode a polypeptide of ≈Mw 258 kDa (SURFIN_4.1) present within the parasitophorous vacuole (PV), around free merozoites as merozoite-associated material, but not at the pRBC-surface. Despite multiple <it>surf</it>_4.1gene copies in some parasites this was not reflected in the levels of SURFIN_4.1polypeptide.</p> <p>Conclusion</p> <p>SURFIN_4.1is a member of the SURFINs, present in the PV and on the released merozoite. The results suggest different SURFINs to be expressed at different locations in the parasite and at distinct time-points during the intra-erythrocytic cycle.</p

The potential of antitranspirants in drought management of arable crops: a review

About 80 % of global farmland is under rain-fed conditions and most of it is prone to drought, which limits crop productivity. Due to climate change, drought will become more frequent and severe, threatening world food security. Antitranspirants, materials that reduce transpiration, could potentially result in greater food production by realising more of a crop’s potential yield during drought. Despite antitranspirants reducing photosynthesis, research has shown that they can mitigate drought stress and increase grain yield. Although this paper is not restricted to specific years, part of it is a systematic review of 173 original research articles published between 2009 and 2018. Overall, the analysis suggests that interest in the potential of antitranspirants is growing. One major achievement in antitranspirant research during the past decade was establishing the optimal timing of application of the substances, which is linked to reproductive processes most vulnerable to drought. Despite research evidence of the efficacy of antitranspirants in ameliorating drought stress, they are not widely used for commercial arable crop production. However, in fruit horticulture, products with antitranspirant effects are already being used commercially for various non-antitranspirant purposes. More recent research shows that with knowledge of a crop's growth stage and soil moisture, antitranspirants can be a valuable option for managing drought impacts on yield in arable crops

Differential var gene expression in the organs of patients dying of falciparum malaria

Sequestration of parasitized erythrocytes in the microcirculation of tissues is thought to be important in the pathogenesis of severe falciparum malaria. A major variant surface antigen, var/Plasmodium falciparum erythrocyte membrane protein 1, expressed on the surface of the infected erythrocyte, mediates cytoadherence to vascular endothelium. To address the question of tissue-specific accumulation of variant types, we used the unique resource generated by the clinicopathological study of fatal paediatric malaria in Blantyre, Malawi, to analyse var gene transcription in patients dying with falciparum malaria. Despite up to 102 different var genes being expressed by P. falciparum populations in a single host, only one to two of these genes were expressed at high levels in the brains and hearts of these patients. These major var types differed between organs. However, identical var types were expressed in the brains of multiple patients from a single malaria season. These results provide the first evidence of organ-specific accumulation of P. falciparum variant types and suggest that parasitized erythrocytes can exhibit preferential binding in the body, supporting the hypothesis of cytoadherence-linked pathogenesis

Multilevel analysis of factors associated with unmet need for family planning among Malawian women

Background: Malawi has a high fertility rate which is also characterized by a relatively high prevalence of unmet need for contraception. However, little is known about the influence of individual- and community- level characteristics on unmet need in Malawi. This study examined the individual- and community- level factors associated with unmet need for family planning (FP) among Malawian women. Methods: Data from the 2015–16 Malawi demographic and health survey were used to analyze 15, 931 women. The association between individual- and community- level factors and unmet need was assessed using multilevel binary logistic regression models. Results: The prevalence of total unmet need was 21.0%. Women aged ≥35 years were more likely to have total unmet need [adjusted odds ratio (aOR) = 1.19, 95% confidence interval (CI) = 1.04–1.35] compared with those aged 15–24 years. Women who were married [aOR = 0.41, 95% CI = 0.35–0.48], and those employed [aOR = 0.78, 95% CI = 0.71–0.85] were associated with less likelihood of having total unmet need compared with unmarried, and unemployed women, respectively. At community-level, women from communities with a high percentage of women from rich households [aOR = 0.81, 95% CI = 0.67–0.96], and those from communities with a middle and high percentage of educated women [aOR = 0.86, 95% CI = 0.76–0.96 and aOR = 0.81, 95% CI = 0.70–0.93, respectively] were less likely to have total unmet need for FP compared with those from communities with low percentages of rich and educated women, respectively. The proportional change in variance showed that about 36.0% of total variations in the odds of unmet need across the communities were explained by both individual- and community-level factors. Moreover, the intraclass correlation showed that about 3.0% of the total variation remained unexplained even after controlling for both individual- and community-level factors. Conclusion: Both individual- and community- level factors influenced unmet need for FP in Malawi. Public health practitioners should conduct community profiling and consider individual and community factors when designing FP programs

On Prioritising Global Health’s Triple Crisis of Sepsis, COVID-19 and Antimicrobial Resistance: A mixed-methods study from Malawi

Sepsis causes 20% of global deaths, particularly among children and vulnerable populations living in developing countries. This study investigated how sepsis is prioritised in Malawi’s health system to inform health policy. In this mixed-methods study, twenty multisectoral stakeholders were qualitatively interviewed and asked to quantitatively rate the likelihood of sepsis-related medium-term policy outcomes being realised. Respondents indicated that sepsis is not prioritised in Malawi due to a lack of local sepsis-related evidence and policies. However, they highlighted strong linkages between sepsis and maternal health, antimicrobial resistance and COVID-19, which are already existing national priorities, and offers opportunities for sepsis researchers as policy entrepreneurs. To address the burden of sepsis, we recommend that funding should be channelled to the generation of local evidence, evidence uptake, procurement of resources and treatment of sepsis cases, development of appropriate indicators for sepsis, adherence to infection prevention and control measures, and antimicrobial stewardship

Comparison of Row Cover Systems for Pest Management in Organic Muskmelon in Iowa

Organic growers of cucurbit (Cucurbitaceae) crops in the midwestern United States have difficulty managing bacterial wilt—a fatal disease with a pathogen (Erwinia tracheiphila) that is transmitted by striped and spotted cucumber beetles (Acalymma vittatum and Diabrotica undecimpunctata howardi, respectively). Registered organic insecticides lack effectiveness, and host plant resistance is rare in commercial cultivars of many cucurbit crops. Row covers are widely used as barriers to minimize pest access, but the spunbonded polypropylene fabric covering traditional low tunnels must be removed at bloom to prevent overheating and facilitate pollination, thereby exposing the crop for the rest of the season. “Mesotunnels”—nylon mesh fabric covering 3.5-ft-high hoops—provide more space than low tunnels and mitigate overheating. In field experiments at Iowa State University (Ames, IA, USA) during 2016–18, two variations of mesotunnels—full-season tunnels [with purchased bumble bees (Bombus impatiens) added for pollination] and part-season tunnels (with covers removed for 2 weeks during bloom to provide pollinator access)—were compared with low tunnels and a noncovered treatment for organic ‘Athena’ muskmelon (Cucumis melo) production. Based on scouting results, full-season mesotunnels required no insecticides and part-season mesotunnels averaged 0.6 spray per season compared with 1.0 and 5.0 sprays per season for the low-tunnel and noncovered treatments, respectively. Incidence of pest and disease damage was zero for the full-season mesotunnels, 5% to 22% for the part-season mesotunnels, and 37% to 70% for both of the other treatments. Marketable yield for the full-season mesotunnel treatment exceeded the noncovered treatment significantly each year, and mean marketable yields were greater numerically than for the other treatments. Both mesotunnel treatments had a marketable yield that averaged more than twice that of the noncovered treatment in each year. Economic analysis (partial budget and cost-efficiency ratio) indicated that mesotunnels were likely to be more profitable in Iowa, USA, than either the low-tunnel or noncovered systems, but also that the year-to-year differential among treatments in profitability could be substantial. Additional experiments are needed to evaluate the efficacy of these integrated pest management practices and their profitability at spatial scales representative of commercial farms

Allele Frequency–Based and Polymorphism-Versus-Divergence Indices of Balancing Selection in a New Filtered Set of Polymorphic Genes in Plasmodium falciparum

Signatures of balancing selection operating on specific gene loci in endemic pathogens can identify candidate targets of naturally acquired immunity. In malaria parasites, several leading vaccine candidates convincingly show such signatures when subjected to several tests of neutrality, but the discovery of new targets affected by selection to a similar extent has been slow. A small minority of all genes are under such selection, as indicated by a recent study of 26 Plasmodium falciparum merozoite-stage genes that were not previously prioritized as vaccine candidates, of which only one (locus PF10_0348) showed a strong signature. Therefore, to focus discovery efforts on genes that are polymorphic, we scanned all available shotgun genome sequence data from laboratory lines of P. falciparum and chose six loci with more than five single nucleotide polymorphisms per kilobase (including PF10_0348) for in-depth frequency–based analyses in a Kenyan population (allele sample sizes >50 for each locus) and comparison of Hudson–Kreitman–Aguade (HKA) ratios of population diversity (π) to interspecific divergence (K) from the chimpanzee parasite Plasmodium reichenowi. Three of these (the msp3/6-like genes PF10_0348 and PF10_0355 and the surf4.1 gene PFD1160w) showed exceptionally high positive values of Tajima's D and Fu and Li's F indices and have the highest HKA ratios, indicating that they are under balancing selection and should be prioritized for studies of their protein products as candidate targets of immunity. Combined with earlier results, there is now strong evidence that high HKA ratio (as well as the frequency-independent ratio of Watterson's θ/K) is predictive of high values of Tajima's D. Thus, the former offers value for use in genome-wide screening when numbers of genome sequences within a species are low or in combination with Tajima's D as a 2D test on large population genomic samples