De novo design of proteins housing excitonically coupled chlorophyll special pairs

Natural photosystems couple light harvesting to charge separation using a ‘special pair’ of chlorophyll molecules that accepts excitation energy from the antenna and initiates an electron-transfer cascade. To investigate the photophysics of special pairs independently of the complexities of native photosynthetic proteins, and as a first step toward creating synthetic photosystems for new energy conversion technologies, we designed C2-symmetric proteins that hold two chlorophyll molecules in closely juxtaposed arrangements. X-ray crystallography confirmed that one designed protein binds two chlorophylls in the same orientation as native special pairs, whereas a second designed protein positions them in a previously unseen geometry. Spectroscopy revealed that the chlorophylls are excitonically coupled, and fluorescence lifetime imaging demonstrated energy transfer. The cryo-electron microscopy structure of a designed 24-chlorophyll octahedral nanocage with a special pair on each edge closely matched the design model. The results suggest that the de novo design of artificial photosynthetic systems is within reach of current computational methods

Overview of the MFTF electrical systems

The Mirror Fusion Test Facility, scheduled for completion in October 1981, will contain a complex, state-of-the-art array of electrical and electronics equipment valued at over 60 M$. Three injector systems will be employed to initiate and sustain the MFTF deuterium plasma. A plasma streaming system and a startup neutron beam system will be used to establish a target plasma. A sustaining neutral beam system will be used to fuel and sustain the MFTF plasma for 0.5 s. Additional power supply systems required on MFTF include two magnet power supplies with quench protection circuitry for powering the superconducting YIN/YANG magnet pair and eight 10 KHz power supplies for powering the Ti gettering system. Due to the complexity, physical size, and multiple systems of MFTF, a distributed, hierarchial, computer control and instrumentation system will be used. Color graphic, touch-panel, control consoles will provide the man-machine interface. The MFTF will have the capability of conducting an experiment every five minutes