Psychometric evaluation of a parental rating scale for estimating language and cognitive functions in preschoolers: A pilot investigation

Στόχος αυτής της πιλοτικής μελέτης είναι η ψυχομετρική διερεύνηση μιας κλίμακας για την αναπτυξιακή εκτίμηση παιδιών 4 έως 7 ετών από τους γονείς τους, με απώτερο στόχο την συμβολή των σχετικών πληροφοριών στην έγκαιρη ανίχνευση μαθησιακών δυσκολιών. Η κλίμακα βασίστηκε στην αγγλική κλίμακα Ratings of Everyday Academic and Cognitive Skills- REACS (Lamb, 2008) και περιλαμβάνει ερωτήσεις για τη γνώση της ονομασίας γραμμάτων, αριθμητικών συμβόλων και επίγνωσης του γραπτού λόγου. Επίσης, περιλαμβάνει ερωτήσεις που αφορούν γνωστικές λειτουργίες όπως βραχύχρονη και μακρόχρονη μνήμη, φωνολογική επεξεργασία, μη λεκτική επικοινωνία και μη λεκτική πρόσληψη λόγου, ικανότητα επίλυσης προβλημάτων και εκτίμηση του ρυθμού μάθησης. Η πιλοτική μελέτη εξέτασε αρχικά δείκτες εσωτερικής συνέπειας και αξιοπιστίας των απαντήσεων ενός τυχαίου δείγματος γονέων (Ν=243), ενώ η διερευνητική ανάλυση παραγόντων επιβεβαίωσε την παραγοντική δομή του αρχικού εργαλείου. Επιπλέον, αξιολογήθηκαν ατομικά 29 παιδιά ηλικίας 4-7 ετών με συστοιχία σταθμισμένων κι άτυπων μετρήσεων γνωστικών και γλωσσικών δεξιοτήτων. Η συγκλίνουσα εγκυρότητα της κλίμακας εκτιμήθηκε με συγκρίσεις μεταξύ των επιδόσεων των παιδιών και τα αποτελέσματα των εκτιμήσεων από τους γονείς τους. Συμπερασματικά, το συγκεκριμένο εργαλείο πληροί σχετικά ψυχομετρικά χαρακτηριστικά χαρακτηριζόμενο από υψηλή εσωτερική συνέπεια και υψηλή σταθερότητα στον χρόνο (αξιοπιστία επανεξέτασης) καθιστώντας το υποψήφιο για εξέταση της συνεισφοράς του στις ανιχνευτικές διαδικασίες που ακολουθούνται για την πρόληψη των μαθησιακών δυσκολιών στο πλαίσιο του γενικού σχολείου.This pilot study examined the psychometric characteristics of a scale to be used by parents of young children for rating cognitive skills and literacy development. The scale was roughly based on the parent form of “Ratings of Everyday Academic & Cognitive Skills” (REACS) (Lamb, 2008) in an effort to develop a cost-effective tool that could potentially increase the predictive validity of early screening assessments. The original scale had been developed to contain three indexes (academic skills, cognitive skills, self-regulation) related to school functioning subscales (Math, Reading, Writing, Language, Learning, Memory, Problem Solving, Attention, Hyperactivity Control, Impulse Control, and Organization). Raters respond to each item on a 6-point scale: 1 = Never, 2 = Rarely, 3 = Sometimes, 4 = Usually, and 5 = Always and 6= Unknown. For this study we collected 243 forms from parents while individualized testing was conducted with 22 Kindergarten children. A subsample of parents (N=44) completed the scale a second time to investigate reliability of their ratings. Test-retest reliability of the raters' estimations and the internal consistency of the scale were examined, while the validity of the Greek scale was established by inspecting the factor structure through exploratory factor analyses. Associations with child achievement measures (letter names and sounds, phonological awareness, vocabulary, invented spelling, word reading, etc.) were also examined. Study's initial findings regarding the psychometric properties of the scale support our claim that it should be used by parents of young children for rating child's cognitive, language and academic skills. They are also discussed within the context of early identification of at-risk children within the school for providing early intervention programs

miRNA Molecules—Late Breaking Treatment for Inflammatory Bowel Diseases?

MicroRNAs (miRNAs) are a group of non-coding RNAs that play a critical role in regulating epigenetic mechanisms in inflammation-related diseases. Inflammatory bowel diseases (IBDs), which primarily include ulcerative colitis (UC) and Crohn’s disease (CD), are characterized by chronic recurrent inflammation of intestinal tissues. Due to the multifactorial etiology of these diseases, the development of innovative treatment strategies that can effectively maintain remission and alleviate disease symptoms is a major challenge. In recent years, evidence for the regulatory role of miRNAs in the pathogenetic mechanisms of various diseases, including IBD, has been accumulating. In light of these findings, miRNAs represent potential innovative candidates for therapeutic application in IBD. In this review, we discuss recent findings on the role of miRNAs in regulating inflammatory responses, maintaining intestinal barrier integrity, and developing fibrosis in clinical and experimental IBD. The focus is on the existing literature, indicating potential therapeutic application of miRNAs in both preclinical experimental IBD models and translational data in the context of clinical IBD. To date, a large and diverse data set, which is growing rapidly, supports the potential use of miRNA-based therapies in clinical practice, although many questions remain unanswered

The Role of Leptin in Childhood Immune Thrombocytopenia (ITP): An Anti-Inflammatory Agent?

To investigate the effect of leptin in childhood ITP, we measured plasma leptin in 39 children with acute ITP, after treatment and in remission, and in 33 healthy age/BMI-matched controls. We also cultured ITP and control peripheral blood mononuclear cells (PBMCs) with recombinant leptin to assess its direct effect on pro/anti-inflammatory cytokine gene expression. A significant increase in leptin was observed in children with active disease compared to controls. A significant inverse correlation of leptin with platelet count was also observed in children with acute ITP. Leptin remained high after treatment with IVIg, whereas steroid treatment lowered leptin below control levels. In remission, leptin was in the control range. Cytokine gene expression was significantly increased in children with acute ITP compared with controls, with highest expression for IFN-γ and IL-10. IVIg/steroid treatment significantly decreased IFN-γ and IL-10 expression. In remission, IFN-γ and IL-10 expression remained low. Addition of leptin to PBMCs isolated from patients in remission resulted in a significant increase in IL-10 gene expression compared to controls. Further experiments with purified T-cells and monocytes identified monocytes as the source of leptin-induced IL-10. We suggest that leptin acts as an active anti-inflammatory agent in childhood ITP by promoting IL-10 secretion by monocytes

Alterations in gut immunological barrier in SARS-CoV-2 infection and their prognostic potential

Although coronavirus disease 2019 (COVID-19) is primarily associated with mild respiratory symptoms, a subset of patients may develop more complicated disease with systemic complications and multiple organ injury. The gastrointestinal tract may be directly infected by SARS-CoV-2 or secondarily affected by viremia and the release of inflammatory mediators that cause viral entry from the respiratory epithelium. Impaired intestinal barrier function in SARS-CoV-2 infection is a key factor leading to excessive microbial and endotoxin translocation, which triggers a strong systemic immune response and leads to the development of viral sepsis syndrome with severe sequelae. Multiple components of the gut immune system are affected, resulting in a diminished or dysfunctional gut immunological barrier. Antiviral peptides, inflammatory mediators, immune cell chemotaxis, and secretory immunoglobulins are important parameters that are negatively affected in SARS-CoV-2 infection. Mucosal CD4+ and CD8+ T cells, Th17 cells, neutrophils, dendritic cells, and macrophages are activated, and the number of regulatory T cells decreases, promoting an overactivated immune response with increased expression of type I and III interferons and other proinflammatory cytokines. The changes in the immunologic barrier could be promoted in part by a dysbiotic gut microbiota, through commensal-derived signals and metabolites. On the other hand, the proinflammatory intestinal environment could further compromise the integrity of the intestinal epithelium by promoting enterocyte apoptosis and disruption of tight junctions. This review summarizes the changes in the gut immunological barrier during SARS-CoV-2 infection and their prognostic potential

Ets-2 Acts As a Transcriptional Repressor of the Human Immunodeficiency Virus Type 1 through Binding to a Repressor–Activator Target Sequence of 5′-LTR

HIV-1 is transcriptionally active in activated T helper (Th)-cells and inactive in naive or resting memory Th-cells. Ets-2 is a preinduction transcriptional repressor of the IL-2 gene in naive Th-cells and a candidate transcriptional repressor of HIV-1 in the same cells, because the −279 to −250 upstream region of HIV-1-LTR [repressor–activator target sequence (RATS)], that participates in HIV-1-LTR transcriptional silencing, encompasses the AAGGAG Ets-2 binding site. In this proof of concept study, we investigated whether Ets-2 represses the expression of HIV-1. To assess whether Ets-2 can repress HIV-1 transcriptional activation acting through RATS, we transfected Jurkat cells with an Ets-2 overexpression plasmid (pCDNA3-ets-2) or Ets-2 silencing plasmids (ets-2-shRNA) and, as target genes, plasmids carrying the whole HIV-1-LTR sequence (HIV-1-LTR-CAT) or two copies of the RATS sequence (2× RATS-CAT) or a point mutation in the Ets-2 binding site (2× mutantRATS-CAT) or CMV-CAT (control). Ets-2 overexpression resulted in a significant reduction of HIV-1-LTR-CAT and 2× RATS-CAT activities in stimulated cells, but not of the 2× mutantRATS-CAT or CMV-CAT. Ets-2 silencing led to increased activities of HIV-1-LTR-CAT and 2× RATS-CAT in unstimulated cells, but had no effect on the activities of 2× mutantRATS-CAT and CMV-CAT. To assess Ets-2 binding to HIV-1-LTR–RATS in naive Th-cells, we isolated naive Th-cell nuclear proteins and passed them through an Ets-2 antibody column; electrophoretic mobility shift assays were performed using an RATS probe mixed with consecutive protein eluates. Ets-2 bound to the HIV-1-LTR–RATS in a dose-dependent manner. To assess Ets-2 binding to RATS in vivo, Jurkat cells were transfected with 2× RATS-CAT and stained for the Ets-2 protein and the RATS sequence by combining immunofluorescence and fluorescence in situ hybridization techniques. In unstimulated cells, Ets-2 bound to RATS, whereas no binding was observed in stimulated cells. To test for RATS specificity, the same experiments were performed with 2× mutantRATS-CAT, and no binding of Ets-2 was observed. The results were corroborated by chromatin immunoprecipitation assays performed with the same cells. Our results show that Ets-2 is a transcriptional repressor of HIV-1. Repression of HIV-LTR-RATS mediated by Ets-2 may account for the low-level transcription and replication of HIV-1 in naive Th-cells, and contribute to the viral latency and maintenance of viral reservoirs in patients, despite long-term therapy

Facing internet fake-medicine and web para-pharmacy in the total absence of official recommendations from medical societies

Purpose: Internet fake information, parapharmacy and counterfeit drugs are a market of hundreds of billion dollars. Misleading internet data decrease patients' compliance to medical care, promote use of questionable and detrimental practices, and jeopardize patient outcome. This is particularly harmful among cancer patients, especially when pain and nutritional aspects are considered. Provision of Web recommendations for the general audience (patients, relatives, general population) from official medical-providers might be useful to outweigh the detrimental internet information produced by non-medical providers. Methods: 370 oncology and anesthesiology related societies were analyzed. Our objective was to evaluate the magnitude of web-recommendation for cancer cachexia and cancer pain for the general audience provided by official medical organizations' web sites at global level. Results: Magnitude of web-recommendations at global level was surprisingly scant both for coverage and consistency. Seven official medical societies provided updated web-recommendation for cancer cachexia to their patients/family members, and 15 for cancer pain. Scantiness was unrelated by continent, developmental index, oncology tradition, economic-geographic area and society type scrutinized. Conclusions: Patients need expert advice when exposed to fake internet information largely dominated by paramedical market profits. In this era of "new media" the patients' net-education represents a new major educational challenge for medical societies

Facing internet fake-medicine and web para-pharmacy in the total absence of official recommendations from medical societies

Purpose: Internet fake information, parapharmacy and counterfeit drugs are a market of hundreds of billion dollars. Misleading internet data decrease patients' compliance to medical care, promote use of questionable and detrimental practices, and jeopardize patient outcome. This is particularly harmful among cancer patients, especially when pain and nutritional aspects are considered. Provision of Web recommendations for the general audience (patients, relatives, general population) from official medical-providers might be useful to outweigh the detrimental internet information produced by non-medical providers. Methods: 370 oncology and anesthesiology related societies were analyzed. Our objective was to evaluate the magnitude of web-recommendation for cancer cachexia and cancer pain for the general audience provided by official medical organizations' web sites at global level. Results: Magnitude of web-recommendations at global level was surprisingly scant both for coverage and consistency. Seven official medical societies provided updated web-recommendation for cancer cachexia to their patients/family members, and 15 for cancer pain. Scantiness was unrelated by continent, developmental index, oncology tradition, economic-geographic area and society type scrutinized. Conclusions: Patients need expert advice when exposed to fake internet information largely dominated by paramedical market profits. In this era of "new media" the patients' net-education represents a new major educational challenge for medical societies