Extraction Optimization, Functional and Thermal Properties of Protein from Cherimoya Seed as an Unexploited By-Product

Plant-based proteins are gaining in attraction compared with animal-based proteins due to their superior ethical profiles, growing concerns on the part of various organizations about animal health and welfare, and increased global greenhouse-gas emissions in meat production. In this study, the response surface methodology (RSM) using a Box-Behnken design (BBD) was applied to optimize the ultrasound-assisted alkaline extraction of cherimoya-seed proteins as valuable by-products. The effects of three pH, temperature, and time factors on the protein-extraction yield and protein content were investigated. The pH at 10.5 and temperature of 41.8 °C for 26.1 min were considered the optimal ultrasound-assisted alkaline-extraction conditions since they provided the maximum extraction yield (17.3%) and protein content (65.6%). An established extraction technique was employed to enhance the cherimoya-seed protein yield, purity, and functional properties. A thermogravimetric analysis (TGA) of the samples showed that the ultrasound-assisted alkaline extraction improved the thermal stability of the protein concentrate

Levels of polycyclic aromatic hydrocarbons in milk and milk powder samples and their likely risk assessment in Iranian population

An MSPE/GC–MS method was used for the measuring of 16 PAHs and assessing of the effect of different factors on PAHs concentrations among different types of dairy products. Moreover, the probabilistic health risk assessment due to ingestion of PAHs by the consumption of milk and milk powder was evaluated.The limit of detection (LOD) and limit of quantitation LOQ were ranged between 0.040-0.075 and 0.121-0.227 μg/kg, respectively. The highest mean of total PAHs was noted in milk powdered (2.28 ± 0.39 μg/kg), while the lowest content was observed in pasteurized milk (0.87 ± 0.18 μg/kg). Except for a few samples of milk powder, the PAHs contents of the other samples was lower than standard limits while the concentration of BaP was lower than of standard levels proposed by EU (0.02-0.06 μg/kg). Considering season effect, the samples in winter had the highest level of PAHs. The percentile 95% actual THQ was the 3.64E-04 value that was lower than 1 value. Hence, the consumers are not at considerable non carcinogenic health risk while the actual ILCR was 3.53E-03 as higher than 1E-04. Therefore, consumers are at considerable carcinogenic risk. Generally speaking, approaching the control plans for a decrease in the concentration of PAHs in dairy products in order to control carcinogenic health risk is crucial

Levels of polycyclic aromatic hydrocarbons in milk and milk powder samples and their likely risk assessment in Iranian population

An MSPE/GC-MS method was used for the measuring of 16 PAHs and assessing of the effect of different factors on PAHs concentrations among different types of dairy products. Moreover, the probabilistic health risk assessment due to ingestion of PAHs by the consumption of milk and milk powder was evaluated. The limit of detection (LOD) and limit of quantitation LOQ were ranged between 0.040-0.075 and 0.121-0.227 mu g/kg, respectively. The highest mean of total PAHs was noted in milk powdered (2.28 +/- 0.39 mu/kg), while the lowest content was observed in pasteurized milk (0.87 +/- 0.18 mu g/kg). Except for a few samples of milk powder, the PAHs contents of the other samples was lower than standard limits while the concentration of BaP was lower than of standard levels proposed by EU (0.02-0.06 mu g/kg). Considering season effect, the samples in winter had the highest level of PAHs. The percentile 95% actual THQ was the 3.64E-04 value that was lower than 1 value. Hence, the consumers are not at considerable non-carcinogenic health risk while the actual ILCR was 3.53E-03 as higher than 1E-04. Therefore, consumers are at considerable carcinogenic risk. Generally speaking, approaching the control plans for a decrease in the concentration of PAHs in dairy products in order to control carcinogenic health risk is crucial8

Extraction Optimization, Functional and Thermal Properties of Protein from Cherimoya Seed as an Unexploited By-Product

Plant-based proteins are gaining in attraction compared with animal-based proteins due to their superior ethical profiles, growing concerns on the part of various organizations about animal health and welfare, and increased global greenhouse-gas emissions in meat production. In this study, the response surface methodology (RSM) using a Box–Behnken design (BBD) was applied to optimize the ultrasound-assisted alkaline extraction of cherimoya-seed proteins as valuable by-products. The effects of three pH, temperature, and time factors on the protein-extraction yield and protein content were investigated. The pH at 10.5 and temperature of 41.8 °C for 26.1 min were considered the optimal ultrasound-assisted alkaline-extraction conditions since they provided the maximum extraction yield (17.3%) and protein content (65.6%). An established extraction technique was employed to enhance the cherimoya-seed protein yield, purity, and functional properties. A thermogravimetric analysis (TGA) of the samples showed that the ultrasound-assisted alkaline extraction improved the thermal stability of the protein concentrate