68 research outputs found
Talla de madurez y relación peso-talla del tiburón de profundidad Etmopterus bigelowi (Squaliformes: Etmopteridae) capturado en el sudeste de Brasil
The blurred lantern shark Etmopterus bigelowi, a deep-water squaloid shark, is globally widespread in temperate and tropical
waters, but there is little available information about its biology owing to its relative rarity of capture and taxonomic confusion with its sibling
species Etmopterus pusillus. Specimens used in this study were collected from July to December 2004 as by-catch in the commercial deepwater
trawl fishery targeting red shrimp Aristaeomorpha foliacea in the southwestern Atlantic. We examined 55 specimens (22 males and
33 females) ranging in total length (TL) from 31.5 to 73 cm and in total weight from 103 to 1600 g. Length-weight relationships were explored
for each sex and significant differences were found in the slope coefficients of the male and female regressions. The size at maturity for each
sex was evaluated fitting a logistic regression. Females matured at larger sizes than males, with estimated sizes at first maturity of 56.0 cm TL
for females and 45.1 cm TL for males. The present study provides important preliminary information about E. bigelowi that can be incorporated
in risk assessment and stock assessment models, essential for efficient management practices aimed at avoiding overexploitation of these
vulnerable deep-sea sharks.El tiburón de profundidad Etmopterus bigelowi se distribuye globalmente en aguas templadas y tropicales; sin embargo, hay poca
información disponible sobre su biología ya que raramente se captura y existe confusión taxonómica con su especie hermana Etmopterus
pusillus. Los ejemplares utilizados en este trabajo se obtuvieron de julio a diciembre de 2004 como captura incidental de la pesca comercial
de arrastre de aguas profundas dirigida al camarón rojo Aristaeomorpha foliacea en el Atlántico sudoccidental. Se examinaron 55 ejemplares
(22 machos y 33 hembras) de 31.5 a 73 cm de longitud total (LT) y de 103 a 1600 g de peso total. Se analizó la relación peso-talla para ambos
sexos, observándose diferencias significativas en las pendientes de las regresiones, tanto de hembras como de machos. La talla de madurez para
cada sexo se evaluó ajustando una regresión logística. La talla de primera madurez de las hembras (56.0 cm LT) fue mayor que la de los machos
(45.1 cm LT). El presente trabajo aporta información preliminar esencial sobre E. bigelowi que puede ser incorporada en modelos de evaluación
de riesgo y evaluación de la población, cruciales para prácticas de gestión dirigidas a evitar la sobreexplotación de estos vulnerables tiburones
de aguas profundas.info:eu-repo/semantics/publishedVersio
Size at maturity and length-weight relationships of the blurred lantern shark Etmopterus bigelowi (Squaliformes: Etmopteridae) caught off southeastern Brazil
The blurred lantern shark Etmopterus bigelowi, a deep-water squaloid shark, is globally widespread in temperate and tropical waters, but there is little available information about its biology owing to its relative rarity of capture and taxonomic confusion with its sibling species Etmopterus pusillus. Specimens used in this study were collected from July to December 2004 as by-catch in the commercial deep-water trawl fishery targeting red shrimp Aristaeomorpha foliacea in the southwestern Atlantic. We examined 55 specimens (22 males and 33 females) ranging in total length (TL) from 31.5 to 73 cm and in total weight from 103 to 1600 g. Length-weight relationships were explored for each sex and significant differences were found in the slope coefficients of the male and female regressions. The size at maturity for each sex was evaluated fitting a logistic regression. Females matured at larger sizes than males, with estimated sizes at first maturity of 56.0 cm TL for females and 45.1 cm TL for males. The present study provides important preliminary information about E. bigelowi that can be incorporated in risk assessment and stock assessment models, essential for efficient management practices aimed at avoiding overexploitation of these vulnerable deep-sea sharks.National Council for Scientific and Technological Development (CNPq, Brazil)info:eu-repo/semantics/publishedVersio
Spatio-temporal distribution and target species in a longline fishery off the southeastern coast of Brazil
No presente estudo, uma análise de agrupamento foi utilizada para classificar 6.486 lances feitos pela frota espinheleira sediada no Estado de São Paulo, Brasil, de 1998 até 2006, em relação à composição das espécies presentes nas capturas. Baseado nas proporções de doze espécies e três grupos de espécies, três agrupamentos foram identificados: C1: outros peixes; C2: tubarão-azul; C3: espadarte. Os resultados indicaram que ao longo do período estudado, a frota direcionou suas capturas principalmente para o tubarão-azul e para o espadarte, além de terem evidenciado que a importância do tubarão-azul nessa pescaria tem crescido progressivamente ao longo dos anos. Áreas mais afastadas da costa foram exploradas principalmente no primeiro e quarto trimestres (em especial a partir de 2001), enquanto que um esforço de pesca mais concentrado perto da quebra da plataforma continental foi observado durante o segundo e terceiro trimestres (para todo o período estudado). A frota espinheleira sediada em São Paulo mudou a estratégia de pesca para diferentes espécies-alvo, o que produziu importantes mudanças nas principais espécies capturadas por esforço de pesca (CPUE). A análise de agrupamento parece ter identificado apropriadamente essas alterações ao longo do tempo, em relação às quais não há, em geral, qualquer informação nos mapas de bordo.In the present study, a cluster analysis, in relation to the species composition of the catches, was used to classify 6,486 fishing sets by a longline fleet based in São Paulo State, Brazil, from 1998 to 2006. Based on the proportions of 12 species and three broader species groups, three clusters were identified: C1: other fishes; C2: blue shark; C3: swordfish. Results indicated that the fleet targeted mainly blue shark and swordfish and also showed that the blue shark importance in this fishery has been growing progressively trough the years. Offshore areas were exploited mainly in the first and fourth quarters (from 2001 mainly), while the fishing effort was more concentrated near the continental shelf break, during the second and third quarters (for the whole period). The longline fishery based in Sao Paulo State changed fishing strategy to target different species which produced important changes in catch-per-unit-effort (CPUE) of the main species caught. Cluster analysis seems to have appropriately identified these changes over time, which is an important information, often missing in logbooks
Short-term movements and habitat preferences of sailfish, Istiophorus platypterus (Istiophoridae), along the southeast coast of Brazil
Anesthesiology Consensus in the Management of the Airway
Os consensos na gestão clínica da via aérea em anestesiologia pretendem disponibilizar informação, baseada na evidência atual ou, na falta desta, na opinião de peritos, no que respeita à abordagem da via aérea difícil previsível ou não previsível.
Reforçamos a importância da avaliação da via aérea e da identificação de potenciais problemas que possam condicionar dificuldade na sua abordagem e a adoção de uma estratégia segura que permita identificar e responder em crescendo de intervenção às dificuldades encontradas. Na impossibilidade de intubação traqueal (não intubo) otimizada e limitada a 4 tentativas, da impossibilidade de ventilar e oxigenar (não oxigeno) após 2 tentativas de usar um dispositivo supraglótico ou de uso de máscara facial inicialmente adequada é importante realizar, em tempo útil, uma cricotirotomia para assegurar oxigenação.
As situações clínicas de exceção só com planos simples, conhecidos por todos e regularmente treinados e adaptados à nossa atividade clinica podem assegurar melhores “outcomes”.
O registo destes eventos e a informação ao nosso doente da dificuldade encontrada e modo como foi resolvido o problema é essencial e constitui ainda um desafio a alargar a uma base nacional.info:eu-repo/semantics/publishedVersio
Adaptively evolved Escherichia coli for improved ability of formate utilization as a carbon source in sugar???free conditions
Background: Formate converted from CO2 reduction has great potential as a sustainable feedstock for biological production of biofuels and biochemicals. Nevertheless, utilization of formate for growth and chemical production by microbial species is limited due to its toxicity or the lack of a metabolic pathway. Here, we constructed a formate assimilation pathway in Escherichia coli and applied adaptive laboratory evolution to improve formate utilization as a carbon source in sugar-free conditions.
Results: The genes related to the tetrahydrofolate and serine cycles from Methylobacterium extorquens AM1 were overexpressed for formate assimilation, which was proved by the 13C-labeling experiments. The amino acids detected by GC/MS showed significant carbon labeling due to biomass production from formate. Then, 150 serial subcultures were performed to screen for evolved strains with improved ability to utilize formate. The genomes of evolved mutants were sequenced and the mutations were associated with formate dehydrogenation, folate metabolism, and biofilm formation. Last, 90 mg/L of ethanol production from formate was achieved using fed-batch cultivation without addition of sugars.
Conclusion: This work demonstrates the effectiveness of the introduction of a formate assimilation pathway, combined with adaptive laboratory evolution, to achieve the utilization of formate as a carbon source. This study suggests that the constructed E. coli could serve as a strain to exploit formate and captured CO2
Photo-Fenton process for treating biological laboratory wastewater containing formaldehyde
Global Women’s Breakfast (GWB): #UnidaspelaQuímica
Global Women’s Breakfast (GWB):
#BoundbyChemistry. Global Women’s
Breakfast is an initiative of the International Union of Pure and Applied Chemistry, aiming to give women scientists, from all over the world, the opportunity to know each other, communicating virtually and sharing their experiences. Many countries joined this initiative and Portugal was not an exception, with its participation already at the first Networking Breakfast in 2011, celebrating the year of the centenaries of Marie Curie Nobel Prize in Chemistry, the Portuguese Chemical Society and the Faculdade de Ciências da Universidade de Lisboa as well. The success of these networking breakfasts, involving students, young researchers, and scientists, encouraged its further organization in Portugal, annually since 2019. This article describes the
interventions of Portuguese women scientists,
coming from Institutions throughout Portugal,
in the Global Women’s Breakfast as partners in their mission as scientists, creative and open to international collaborations.
Global Women's Breakfast é uma iniciativa
criada pela International Union of Pure and
Applied Chemistry para dar oportunidade
às mulheres cientistas de todo o mundo de
se conhecerem, comunicando virtualmente
e compartilhando as suas experiências.
A adesão dos países foi muito elevada e
Portugal não foi exceção, participando já
no primeiro Networking Breakfast em 2011,
Ano Internacional da Química, no qual se
celebraram os centenários do Prémio Nobel
da Química a Marie Curie, da Sociedade
Portuguesa de Química e da Faculdade de
Ciências da Universidade de Lisboa. O sucesso
destes pequenos-almoços em rede, que
envolvem estudantes, jovens investigadoras
e cientistas, encorajou a continuação da
participação de Portugal anualmente, desde
2019. Este artigo descreve a intervenção de
mulheres cientistas portuguesas, pertencentes
a instituições de Norte a Sul do país, no
Global Women’s Breakfast, cúmplices na sua
missão de cientistas, criativas e abertas à
colaboração internacional
Carbon Dioxide Utilisation -The Formate Route
UIDB/50006/2020 CEEC-Individual 2017 Program Contract.The relentless rise of atmospheric CO2 is causing large and unpredictable impacts on the Earth climate, due to the CO2 significant greenhouse effect, besides being responsible for the ocean acidification, with consequent huge impacts in our daily lives and in all forms of life. To stop spiral of destruction, we must actively reduce the CO2 emissions and develop new and more efficient “CO2 sinks”. We should be focused on the opportunities provided by exploiting this novel and huge carbon feedstock to produce de novo fuels and added-value compounds. The conversion of CO2 into formate offers key advantages for carbon recycling, and formate dehydrogenase (FDH) enzymes are at the centre of intense research, due to the “green” advantages the bioconversion can offer, namely substrate and product selectivity and specificity, in reactions run at ambient temperature and pressure and neutral pH. In this chapter, we describe the remarkable recent progress towards efficient and selective FDH-catalysed CO2 reduction to formate. We focus on the enzymes, discussing their structure and mechanism of action. Selected promising studies and successful proof of concepts of FDH-dependent CO2 reduction to formate and beyond are discussed, to highlight the power of FDHs and the challenges this CO2 bioconversion still faces.publishersversionpublishe
- …