6,872 research outputs found
Output functions and fractal dimensions in dynamical systems
We present a novel method for the calculation of the fractal dimension of
boundaries in dynamical systems, which is in many cases many orders of
magnitude more efficient than the uncertainty method. We call it the Output
Function Evaluation (OFE) method. The OFE method is based on an efficient
scheme for computing output functions, such as the escape time, on a
one-dimensional portion of the phase space. We show analytically that the OFE
method is much more efficient than the uncertainty method for boundaries with
, where is the dimension of the intersection of the boundary with a
one-dimensional manifold. We apply the OFE method to a scattering system, and
compare it to the uncertainty method. We use the OFE method to study the
behavior of the fractal dimension as the system's dynamics undergoes a
topological transition.Comment: Uses REVTEX; to be published in Phys. Rev. Let
Beyond Counting: New Perspectives on the Active IPv4 Address Space
In this study, we report on techniques and analyses that enable us to capture
Internet-wide activity at individual IP address-level granularity by relying on
server logs of a large commercial content delivery network (CDN) that serves
close to 3 trillion HTTP requests on a daily basis. Across the whole of 2015,
these logs recorded client activity involving 1.2 billion unique IPv4
addresses, the highest ever measured, in agreement with recent estimates.
Monthly client IPv4 address counts showed constant growth for years prior, but
since 2014, the IPv4 count has stagnated while IPv6 counts have grown. Thus, it
seems we have entered an era marked by increased complexity, one in which the
sole enumeration of active IPv4 addresses is of little use to characterize
recent growth of the Internet as a whole.
With this observation in mind, we consider new points of view in the study of
global IPv4 address activity. Our analysis shows significant churn in active
IPv4 addresses: the set of active IPv4 addresses varies by as much as 25% over
the course of a year. Second, by looking across the active addresses in a
prefix, we are able to identify and attribute activity patterns to network
restructurings, user behaviors, and, in particular, various address assignment
practices. Third, by combining spatio-temporal measures of address utilization
with measures of traffic volume, and sampling-based estimates of relative host
counts, we present novel perspectives on worldwide IPv4 address activity,
including empirical observation of under-utilization in some areas, and
complete utilization, or exhaustion, in others.Comment: in Proceedings of ACM IMC 201
NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo–Cu cluster
Biomol NMR Assign (2007) 1:81–83
DOI 10.1007/s12104-007-9022-3We report the 98% assignment of the apo-form
of an orange protein, containing a novel Mo–Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments
Energy metabolism in human pluripotent stem cells and their differentiated counterparts
Background: Human pluripotent stem cells have the ability to generate all cell types present in the adult organism, therefore harboring great potential for the in vitro study of differentiation and for the development of cell-based therapies. Nonetheless their use may prove challenging as incomplete differentiation of these cells might lead to tumoregenicity. Interestingly, many cancer types have been reported to display metabolic modifications with features that might be similar to stem cells. Understanding the metabolic properties of human pluripotent stem cells when compared to their differentiated counterparts can thus be of crucial importance. Furthermore recent data has stressed distinct features of different human pluripotent cells lines, namely when comparing embryo-derived human embryonic stem cells (hESCs) and induced pluripotent stem cells (IPSCs) reprogrammed from somatic cells. Methodology/Principal Findings: We compared the energy metabolism of hESCs, IPSCs, and their somatic counterparts. Focusing on mitochondria, we tracked organelle localization and morphology. Furthermore we performed gene expression analysis of several pathways related to the glucose metabolism, including glycolysis, the pentose phosphate pathway and the tricarboxylic acid (TCA) cycle. In addition we determined oxygen consumption rates (OCR) using a metabolic extracellular flux analyzer, as well as total intracellular ATP levels by high performance liquid chromatography (HPLC). Finally we explored the expression of key proteins involved in the regulation of glucose metabolism. Conclusions/Findings: Our results demonstrate that, although the metabolic signature of IPSCs is not identical to that of hESCs, nonetheless they cluster with hESCs rather than with their somatic counterparts. ATP levels, lactate production and OCR revealed that human pluripotent cells rely mostly on glycolysis to meet their energy demands. Furthermore, our work points to some of the strategies which human pluripotent stem cells may use to maintain high glycolytic rates, such as high levels of hexokinase II and inactive pyruvate dehydrogenase (PDH). © 2011 Varum et al
Determinação da atividade de invertase em extratos enzimáticos.
bitstream/CNPAT-2010/10231/1/Dc-108.pd
The Vampire and the FOOL
This paper presents new features recently implemented in the theorem prover
Vampire, namely support for first-order logic with a first class boolean sort
(FOOL) and polymorphic arrays. In addition to having a first class boolean
sort, FOOL also contains if-then-else and let-in expressions. We argue that
presented extensions facilitate reasoning-based program analysis, both by
increasing the expressivity of first-order reasoners and by gains in
efficiency
Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of glycerol membrane permeability
© The Animal Consortium 2011In the meat industry, the manipulation of fat deposition in cattle is of pivotal importance to improve production efficiency, carcass composition and ultimately meat quality. There is an increasing interest in the identification of key factors and molecular mechanisms responsible for the development of specific fat depots. This study aimed at elucidating the influence of breed and diet on adipose tissue membrane permeability and fluidity and their interplay on fat deposition in bovines. Two Portuguese autochthonous breeds, Alentejana and Barrosã, recognized as late- and early-maturing breeds, respectively, were chosen to examine the effects of breed and diet on fat deposition and on adipose membrane composition and permeability. Twenty-four male bovines from these breeds were fed on silage-based or concentrate-based diets for 11 months. Animals were slaughtered to determine their live slaughter and hot carcass weights, as well as weights of subcutaneous and visceral adipose depots. Mesenteric fat depots were excised and used to isolate adipocyte membrane vesicles where cholesterol content, fatty acid profile as well as permeability and fluidity were determined. Total accumulation of neither subcutaneous nor visceral fat was influenced by breed. In contrast, mesenteric and omental fat depots weights were higher in concentrate-fed bulls relative to silage-fed animals. Membrane fluidity and permeability to water and glycerol in mesenteric adipose tissue were found to be independent of breed and diet. Moreover, the deposition of cholesterol and unsaturated fatty acids, which may influence membrane properties, were unchanged among experimental groups. Adipose membrane lipids from the mesenteric fat depot of ruminants were rich in saturated fatty acids, and unaffected by polyunsaturated fatty acids dietary levels. Our results provide evidence against the involvement of cellular membrane permeability to glycerol on fat accumulation in mesenteric fat tissue of concentrate-fed bovines, which is consistent with the unchanged membrane lipid profile found among experimental groups.This study was supported by Fundação para a Ciência e a Tecnologia (FCT) through grant PTDC/CVT/2006/66114 and individual fellowships to Ana P. Martins (SFRH/BD/2009/65046), Ana S. H. Costa (SFRH/BD/2009/61068) and Susana V. Martins (SFRH/BPD/2009/63019). Paula A. Lopes is a researcher from the program ‘‘Ciência 2008’’ from FC
Molybdenum Induces the expression of a protein containing a new heterometallic Mo-Fe cluster in desulfoVibrio alaskensis
Biochemistry. 2009 Feb 10;48(5):873-82. doi: 10.1021/bi801773t.The characterization of a novel Mo-Fe protein (MorP) associated with a system that responds to Mo in Desulfovibrio alaskensis is reported. Biochemical characterization shows that MorP is a periplasmic homomultimer of high molecular weight (260 +/- 13 kDa) consisting of 16-18 monomers of 15321.1 +/- 0.5 Da. The UV/visible absorption spectrum of the as-isolated protein shows absorption peaks around 280, 320, and 570 nm with extinction coefficients of 18700, 12800, and 5000 M(-1) cm(-1), respectively. Metal content, EXAFS data and DFT calculations support the presence of a Mo-2S-[2Fe-2S]-2S-Mo cluster never reported before. Analysis of the available genomes from Desulfovibrio species shows that the MorP encoding gene is located downstream of a sensor and a regulator gene. This type of gene arrangement, called two component system, is used by the cell to regulate diverse physiological processes in response to changes in environmental conditions. Increase of both gene expression and protein production was observed when cells were cultured in the presence of 45 microM molybdenum. Involvement of this system in Mo tolerance of sulfate reducing bacteria is proposed
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