Biometric Indicators and Yield of Tomato under Conventional and Unconventional Biostimulators

The research consisted of an experiment applying unconventional stimulators to tomato crop, as a measure against chemical stimulators, known as having negative effects on human health. In our research there have been used four unconventional stimulators (Ecostim, AuCl4-50μg, AuCl4-30μg and Chitosan) and a conventional stimulator BNOA, all compared with the untreated control.Application of stimulators in unconventional farming determined lower productions compared to conventional farming, but is an alternative because determined healthy products. In three of the four unconventional variants, the content of macro- and microelements in plants was higher

Mixture support for essential elements and its effect on Zn and Cd sorption

Zinc and Cadmium are elements similar in chemical properties but different in mobility and availability, used as stress factors and contaminants. The aim of the study was to establish the sorption capacity of Zn (II) and Cd (II) on a nutrient mixture, the conditions under which the process is maximal and the changes that undergo in the chemical composition of the mixture. The mixture consists in eutrophic peat and compost (60:40%), which contains in addition to organic matter, macronutrients (N, P, K) and certain content of inorganic contaminants (Ni, Pb, Co, Cd, Zn). The parameters that influence the sorption process on nutrient mixture were: initial solution pH (1.0-9.0), contact time (0-1200 min), Zn and Cd concentrations (50-250 mg/l) and organic matter content (52%). The results show that certain characteristics such as phosphorus and potassium content did not change significantly, while the organic matter content decreased to 45%. The humic acids from the organic matter composition caused a significant immobilization of Zn (II) and only a slight immobilization of Cd (II). The values of Cd and Zn from nutritive solution are higher (42.24 mg/kg Zn and 11.69 mg/kg C) than accessible fraction (19.26 mg/kg Zn and 1.4675 mg/kg Cd). The heavy metals content decreases with the increase of pH (5.0 mg/kg Cd, 3.0 mg/kg Zn). The content of accessible fraction reveal reduced values of Cd and Zn that does not represent a threat for human health. These organic materials improve soil fertility, can change the availability of heavy metals and increase crop production

Advanced Design for Experimental Optimisation of Physico-Mechanical Characteristics of Sustainable Local Hemp Concrete

The meaning of technological progress is to produce economic development and to increase the level of personal comfort. Sustainability can only be achieved if, at the microsystem level as well as at the macrosystem level, the secondary effects of the activities undertaken by people on the environment are in a state of neutrality compared to the impact they can produce on natural conditions. This neutrality can be intrinsic or can be achieved through coercive and compensatory measures. If we take into account the production of carbon dioxide that accompanies a product from the stages of conceptualisation, design, procurement of materials, execution, operation, maintenance, decommissioning and recycling the waste produced at the end of use, then nothing can be sustainable in pure form. Nevertheless, there are products whose production, both as a raw material and as a technological process, can be neutral in terms of carbon emissions. Moreover, they can even become carbon negative over time. This is also the case with eco-sustainable hemp concrete, whose capacity to absorb carbon dioxide starts from the growth phase of the plant from which the raw material is obtained and continues throughout the existence of the constructed buildings. Not only does it absorb carbon dioxide, but it also stores it for a period of at least 50 years as long as the construction is guaranteed, being at the same time completely recyclable. However, in order to obtain an optimal mixture from the point of view of raw material consumption, represented by industrial hemp wood chips and the binder based on lime and cement, multiple experiments are necessary. The study presented in this work is based on the use of an advanced method of experimental planning (design of experiments method), which makes possible the correlation between the values obtained experimentally and the algorithm that generated the matrix arrangement of the quantities of materials used in the recipes. This approach helps to create the necessary framework for parametric optimisation with a small number of trials. Thus, it is possible to obtain the mathematical law valid within the minimum and maximum limits of the studied domain that defines the characteristics of the material and allows the achievement of optimisation. The material is thus designed to satisfy the maximum thermal insulation requirements that it can achieve depending on a certain minimum admissible compressive strength

Influence of Environmental Factors on Some Biochemical and Physiological Indicators in Grapevine from Copou Vineyard, Iasi, Romania

Climate factors strongly impact the growth of grapes and their flavonoid composition, especially due to heat and drought stress. Four varieties—Gelu, Moldova, Purpuriu, and Coarna Neagra—from a Copou vineyard were analyzed to assess the impact of climate change on the anthocyanin concentration in grapes and total chlorophyll content of the leaves, and find possible correlations between these parameters during the two studied years, such as to raise producer awareness regarding the climate impact on table grape vine growth. Moldova and Purpuriu show adaptation to a slight temperature increase above the normal average and also to a water deficit, with increased concentrations of anthocyanins of up to three times. The Moldova variety accumulated low amounts of chlorophyll pigments in most analyzed development phases, indicating a negative correlation between these pigments. Coarna neagra exhibited an increase of the foliar pigments, a variety that proved to be less valuable from the point of view of anthocyanin content. Positive correlations were found between the anthocyanin and chlorophyll concentrations in the case of Gelu, Moldova, and Coarna neagra cultivars during the latter phase of the fruit development, whereas the water deficit in the second year induced negative correlations during fruit development and at the time of harvest for all studied varieties except Moldova

Chitosan-Based Delivery Systems Loaded with Glibenclamide and Lipoic Acid: Formulation, Characterization, and Kinetic Release Studies

Glibenclamide and lipoic acid are two drugs frequently recommended for the management of diabetes mellitus, and so, the development of a new formulation containing both substances has a great benefit in terms of efficiency and compliance, acting also as a multi-target drug system. Accordingly, the aim of this study was the formulation and physicochemicalcharacterization of new polymeric systems based on chitosan (CS) in whose matrix were encapsulated glibenclamide (Gly) and lipoic acid (LA). The polymeric systems were prepared as microparticles (CS–Gly, CS–LA, and CS–Gly–LA) through ionic gelation method, using pentasodium tripolyphosphate (TPP) as crosslinking agent. The polymeric systems obtained were characterized in terms of particle size and morphology, IR spectroscopy, entrapment efficiency and drug loading, swelling degree, and therelease of the active substances from the chitosan matrix. The polymeric systems obtained were stable systems; the presence of glibenclamide and lipoic acid into the polymer matrix were proved by IR spectroscopy. The entrapment efficiency was 94.66% for Gly and 39.68% for LA. The developed polymeric systems proved a favorable swelling degree and drug release profile, the percentage of release being 88.68% for LA and 75.17% for Gly from CS–Gly–LA systems

The impact of salinity stress on antioxidant response and bioactive compounds of Nepeta cataria L.

In this study, the ability of Nepeta cataria L. to grow and synthesize bioactive compounds on soil treated with different salt concentrations was tested to evaluate the opportunity of cultivating it in soils affected by salinization. N. cataria L. was grown in soil containing specific amounts of NaCl, Na2SO4, and their mixture. After harvesting, the plants were analyzed from the morphological and physiological point of view. Salinity stress inhibited the growth, with the highest decrease of the plant yield up to about 70% in the case of salt mixture, and smaller values for the separate salt treatments. In the same time, as a defense mechanism, there was an increase of granular trichomes’ density, as observed with the scanning electron microscope. For mild concentrations of salt, the amount of chlorophyll pigments was enhanced, while for stronger salinity stress, it decreased. The opposite behavior was evidenced for the polyphenol content, as antioxidant activity was used as a protective mechanism against reactive oxygen species produced under salinity stress. The antioxidant activity was considerably higher for separate NaCl and Na2SO4 treatments than for the salt mixture variants. The results showed that the species Nepeta cataria L. reacts well to high salinity levels, with an increased content of bioactive compounds and antioxidant activity even for the highest studied salinity conditions

Salinity Stress Influences the Main Biochemical Parameters of Nepeta racemosa Lam.

In this work, the effects of salt stress on Nepeta racemosa Lam. were studied to analyze the possibility of using it as a potential culture for salinity-affected soils. A total of nine concentrations of salts—NaCl (18, 39, and 60 mg/100 g soil), Na2SO4 (50, 85, and 120 mg/100 g soil), and a mixture (9 g NaCl + 25 g Na2SO4, 19 g NaCl + 43 g Na2SO4, and 30 g NaCl + 60 g Na2SO4/100 g soil)—simulated real salinity conditions. Environmental electron microscopy offered information about the size and distribution of glandular trichomes, which are very important structures that contain bioactive compounds. The chlorophyll pigments, polyphenols, flavonoids, and antioxidant activity were determined based on spectrophotometric protocols. The results have shown a different impact of salinity depending on the salt type, with an increase in bioactive compound concentrations in some cases. The highest polyphenol concentrations were obtained for Na2SO4 variants (47.05 and 46.48 mg GA/g dw for the highest salt concentration in the first and second year, respectively), while the highest flavonoid content was found for the salt mixtures (42.77 and 39.89 mg QE/g dw for the highest concentrations of salt in the first and, respectively, the second year), approximately 100% higher than control. From the Pearson analysis, strong correlations were found between chlorophyll pigments (up to 0.93), antioxidant activity and yield for the first harvest (up to 0.38), and antioxidant activity and flavonoid content for the second harvest (up to 0.95). The results indicate the possibility of growing the studied plants in salt-stress soils, obtaining higher concentrations of bioactive compounds

Prenylated phenolics as promising candidates for combination antibacterial therapy: morusin and kuwanon G

Combination of antibiotics with natural products is a promising strategy for potentiating antibiotic activity and overcoming antibiotic resistance. The purpose of the present study was to investigate whether morusin and kuwanon G, prenylated phenolics in Morus species, have the ability to enhance antibiotic activity and reverse antibiotic resistance in Staphylococcus aureus and Staphylococcus epidermidis. Commonly used antibiotics (oxacillin, erythromycin, gentamicin, ciprofloxacin, tetracycline, clindamycin) were selected for the combination studies. Checkerboard and time-kill assays were used to investigate potential bacteriostatic and bactericidal synergistic interactions, respectively between morusin or kuwanon G and antibiotics. According to both fractional inhibitory concentration index and response surface models, twenty combinations (14 morusin-antibiotic combinations, six kuwanon G-antibiotic combinations) displaying bacteriostatic synergy were identified, with 4–512-fold reduction in the minimum inhibitory concentration values of antibiotics in combination. Both morusin and kuwanon G reversed oxacillin resistance of methicillin-resistant Staphylococcus aureus. In addition, morusin reversed tetracycline resistance of Staphylococcus epidermidis. At half of the minimum inhibitory concentrations, combinations of morusin with oxacillin or gentamicin showed bactericidal synergy against methicillin-resistant Staphylococcus aureus. Fluorescence and differential interference contrast microscopy and scanning electron microscopy showed an increase in the membrane permeability and massive leakage of cellular content in methicillin-resistant Staphylococcus aureus exposed to morusin or kuwanon G. Overall, our findings strongly indicate that both prenylated compounds are good candidates for the development of novel antibacterial combination therapies

Mesenchymal stem cells increase proliferation but do not change quiescent state of osteosarcoma cells: Potential implications according to the tumor resection status

Conventional therapy of primary bone tumors includes surgical excision with wide resection, which leads to physical and aesthetic defects. For reconstruction of bone and joints, allografts can be supplemented with mesenchymal stem cells (MSCs). Similarly, adipose tissue transfer (ATT) is supplemented with adipose-derived stem cells (ADSCs) to improve the efficient grafting in the correction of soft tissue defects. MSC-like cells may also be used in tumor-targeted cell therapy. However, MSC may have adverse effects on sarcoma development. In the present study, human ADSCs, MSCs and pre-osteoclasts were co-injected with human MNNG-HOS osteosarcoma cells in immunodeficient mice. ADSCs and MSCs, but not the osteoclast precursors, accelerated the local proliferation of MNNG-HOS osteosarcoma cells. However, the osteolysis and the metastasis process were not exacerbated by ADSCs, MSCs, or pre-osteoclasts. In vitro proliferation of MNNG-HOS and Saos-2 osteosarcoma cells was increased up to 2-fold in the presence of ADSC-conditioned medium. In contrast, ADSC-conditioned medium did not change the dormant, quiescent state of osteosarcoma cells cultured in oncospheres. Due to the enhancing effect of ADSCs/MSCs on in vivo/in vitro proliferation of osteosarcoma cells, MSCs may not be good candidates for osteosarcoma-targeted cell therapy. Although conditioned medium of ADSCs accelerated the cell cycle of proliferating osteosarcoma cells, it did not change the quiescent state of dormant osteosarcoma cells, indicating that ADSC-secreted factors may not be involved in the risk of local recurrence

Osseointegration evaluation of ZrTi alloys with hydroxyapatite-zirconia-silver layer in pig's tibiae

In this work we studied the main surface characteristics together with the in vivo osseointegration of three Zr-Ti alloys with hydroxyapatite-zirconia-silver (HAP-ZrO2- Ag) coating layer. The main purpose was to provide a quantitative evaluation and interpretation from micro to nano scale of the processes involved in the osseointegration of the HAP-ZrO2-Ag coated Zr-Ti specimens in a pig model. The surface characteristics were studied by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). After the insertion of the implants into pig tibia, the osseointegration was investigated by means of biochemical analysis as well as bone histomorphometric and computed tomography measurements. The results of this in vivo study sustains that the capacity of the Zr-Ti specimens coated with HAPZrO2- Ag to promote osteogenesis is significant during the first month after implantation as compared with the control, being influenced by the interactions between bone tissue and HAP-ZrO2-Ag surfaces, but also by the chemical composition of the alloys