Tools for optimal operation and planning or urban distribution systems

This paper reports on the results of the implementation of a set of software tools for the comprehensive analysis and optimisation of distribution systems, developed jointly by Azienda Energetica Metropolitana (AEM) Torino S.p.A. and Dipartimento di Ingegneria Elettrica Industriale - Politecnico di Torino. The software tools cover several aspects of distribution system analysis, fault current calculation, reliability assessment, total operating cost evaluation, optimal reconfiguration, optimal planning and service restoration

Life of man on the High Alps

Analyse: Chapter 23: The new observatory and alpine station on Monte Rosa (Regina Margherita)

Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells

Current methods for dengue virus quantitation are either time consuming, technically demanding or costly. As an alternative, the commercial enzyme immunoassay Platelia™ Dengue NS1 AG (Bio-Rad Laboratories) was used to monitor semiquantitatively dengue virus replication in cultured cells. The presence of NS1 protein was evaluated in supernatants from Vero and C6/36 HT cells infected with dengue virus. The amount of NS1 detected in the supernatants of infected cells was proportional to the initial MOI used and to the time of post infection harvest. This immunoassay was also able to detect the presence of NS1 in the supernatants of infected human macrophages. Inhibition of dengue virus replication in C6/36 HT cells treated with lysosomotropic drugs was readily monitored with the use of this assay. These results suggest that the Platelia™ Dengue NS1 AG kit can be used as a fast and reliable surrogate method for the relative quantitation of dengue virus replication in cultured cells

Cytochrome P450 from Plants: Platforms for Valuable Phytopharmaceuticals

Cytochrome P450 enzymes are important for biotechnology due to their capacity to modify diverse secondary metabolites that may produce chemicals with pharmacological properties. Most terpenes, flavonoids and alkaloids require P450 catalytic functions to reach their biological activity. In the last ten years, several efforts have focused on the expression and production of these three main types of secondary metabolites in engineered microorganisms and plants using P450 of ethnobotanical origin. Despite this, several P450 coding sequences from plant sources are discovered yearly but only a few have been screened by functional genomics. Amongst them, only a few have shown potentials for use in sustainable production of novel drugs and highly valuable products. Cytochrome P450 involvement in the biosynthesis of these products is discussed in this work.Keywords: Biotechnological platforms, Cytochrome P450, Phytopharmaceuticals, Yield improvement, Terpenes, Flavonoids, Alkaloids, Microbial expressio

Quantifying the contribution of single joint kinematics to the overall ergonomic discomfort

Work-related musculoskeletal disorders (WMSDs) represent one of the major issues concerning the occupational safety and health of workers. Thus, a reliable evaluation of workers’ exposure to the risk factors that may contribute to WMSDs development is mandatory, above all, within an industrial context. At present, standard synthetic indices are widely used in this frame, presenting – however - several limitations due to poor reliability and time efficiency. The aim of this work was to investigate the contribution of the displacement quantified for each single joint during the execution of simple reaching tasks, to the overall discomfort of the worker evaluated by means of standard observational methods. Forty-five healthy volunteers were included in the analysis; each subject was asked to reach and rotate 2 spheres placed on a custom-made rack in standardized positions, i.e., above the head and one at floor level at centre side. Whole-body kinematics was acquired via a system based on wearable inertial measurement units. Standard ergonomic scales including RULA (Rapid Upper Limb Assessment), REBA (Rapid Entire Body Assessment), and MMGA (Method for Movement and Gesture Assessment), were assessed for each subject and each sphere position. Moreover, a quantitative index based on actual joint kinematics, i.e., W1 index, was computed for each joint angle involved in the task. Correlation analysis was performed for W1 relative to each joint with respect to RULA, REBA, and MMGA scores. Considering REBA and MMGA scores, the most comfortable reaching areas were the ones in which the sphere was positioned at the top; in contrast, the lowest positions evidenced the most increased discomfort indexes. The RULA did not result sensitive to the different positions, while REBA and MMGA seemed to be more influenced by the range of motion of the lower limb joint angles than the upper limb ones. This study underlines the necessity to focus on multiple potential contributors to WMSDs and underlines the importance of subject-specific approaches toward risk assessment by exploiting quantitative measurements and wearable technologies, which indeed represent key enabling approaches even in consideration of the novel “Industry 5.0” perspective

Recent distribution of Red-legged Partridge Alectoris rufa in Piedmont (North Western Italy): signs of recent spreading.

The red-legged partridge Alectoris rufa in Piedmont (NW Italy) is historically limited to the hilly and low-mountain areas of the southern half of the region, in particular lying in the Langhe and the Apennines on the Ligurian border, in the districts of Cuneo, Asti and Alessandria. In the Cuneo district, on the Maritime Alps, hybrid populations with Alectoris graeca were also known. The distribution of the species has been mapped in some occasions with the \u201cAtlases\u201d projects of the GPSO (Gruppo Piemontese Studi Ornitologici) and special enquiries. On this occasion we tried to update the geographical distribution of the species using data conferred by bird-watchers on www.regione.piemonte.it/aves/, managed by the GPSO, enquiries among hunters\u2019 organizations and specific field researches. The resulting distribution is shown on a 10 km square grid. The species shows signs of distribution dynamism, with the occupation of some lowland areas, where it was totally absent until the \u201890s. A little range expansion is noticeable also in the Western Monferrato. The situation seems to be more stable (or even declining) in the Langhe area and the species is now disappeared from the alpine border. In general we are seeing an areal contraction or density reduction on hills and mountains and an extension on lowlands. The observed changes seem to be attributable mainly to environmental and climate changes

Quantum effects:heat flow in atomic bottlenecks

The Wiedemann–Franz law linking electrical and thermal conductance has now been verified experimentally in atomic junctions