Pinpointing Key Dates in Jesus’ Life: His Birth, the Start of His Ministry, and His Crucifixion

The abundant chronological details embedded in Scripture, along with external time markers in other ancient histories and in astronomical events, persuasively support the view that Jesus was born in mid-June, 2 BC, not on December 25, 4 BC, and that His ministry endured not for three years, as so often claimed, but for 1,350 days or nearly four years, from His baptism on Sunday, 7 Elul, September 2, AD 29 to His ascension on Friday, 26 Iyar, May 13, AD 33

An Expanded View of the Eukaryotic Cytoskeleton

A rich and ongoing history of cell biology research has defined the major polymer systems of the eukaryotic cytoskeleton. Recent studies have identified additional proteins that form filamentous structures in cells and can self-assemble into linear polymers when purified. This suggests that the eukaryotic cytoskeleton is an even more complex system than previously considered. In this essay, I examine the case for an expanded definition of the eukaryotic cytoskeleton and present a series of challenges for future work in this area

Does performance information about public services affect citizens' perceptions, satisfaction, and voice behaviour? Field experiments with absolute and relative performance information

© 2014 John Wiley & Sons LtdThis is the peer reviewed version of the following article: James. O. and Moseley, A. 2014. ‘Does Performance Information about Public Services Affect Citizens’ Perceptions, Satisfaction and Voice Behaviour? Field Experiments with Absolute and Relative Performance Information’, Public Administration, 92(2): 493-511, which has been published in final form at doi: 10.1111/padm.12066. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.We evaluate a theory of the effects of publishing performance information on citizens' collective voice to local providers about public service performance and the perceptions and attitudes that influence their voice. Field experiments show that information about low absolute and relative performance of local government household waste recycling services lowers citizens' perceptions of performance, and information about high absolute and relative performance raises perceived performance. Relative information makes citizens judge local providers as being more responsible for outcomes in the case of high performance, suggesting that systems for comparative performance reporting increase local accountability for outcomes. Negativity bias is evident, with information about low absolute performance reducing citizens' satisfaction but information about high performance not raising satisfaction. Information about low performance did not trigger collective voice protest behaviour as hypothesized, suggesting that providers who need citizens' collective voice the most do not get it. © 2014 John Wiley & Sons Ltd.Economic and Social Research Counci

Eisosomes Provide Membrane Reservoirs for Rapid Expansion of the Yeast Plasma Membrane

Cell surface area rapidly increases during mechanical and hypoosmotic stresses. Such expansion of the plasma membrane requires \u27membrane reservoirs\u27 that provide surface area and buffer membrane tension, but the sources of this membrane remain poorly understood. In principle, the flattening of invaginations and buds within the plasma membrane could provide this additional surface area, as recently shown for caveolae in animal cells. Here, we used microfluidics to study the rapid expansion of the yeast plasma membrane in protoplasts, which lack the rigid cell wall. To survive hypoosmotic stress, yeast cell protoplasts required eisosomes, protein-based structures that generate long invaginations at the plasma membrane. Both budding yeast and fission yeast protoplasts lacking eisosomes were unable to expand like wild-type protoplasts during hypoosmotic stress, and subsequently lysed. By performing quantitative fluorescence microscopy on single protoplasts, we also found that eisosomes disassembled as surface area increased. During this process, invaginations generated by eisosomes at the plasma membrane became flattened, as visualized by scanning electron microscopy. We propose that eisosomes serve as tension-dependent membrane reservoirs for expansion of yeast cells in an analogous manner to caveolae in animal cells

Naval Postgraduate School NPSAT1 Small Satellite

Paper presented at the ESA Small Satellite Systems and Services SymposiumThe NPSAT1 mission, conceived and developed by the Naval Postgraduate School (NPS) Space Systems Academic Group (SSAG), is sponsored and executed by the DoD Space Test Program (SMC SDD). The small satellite is manifested for launch aboard the STP-1 Atlas V Mission due to launch in December 2006. The main objective of the NPSAT1 program is to provide educational opportunities for the offi cer students in the Space Systems Curricula at NPS through the design, testing, integration, and fl ight operations of a small satellite. The 82 kg (180 lbs) satellite will be earth-pointing using a novel, low-cost, 3-axis attitude control scheme. NPSAT1 will provide a platform for a number of spacecraft technology experiments, including a lithium-ion battery, a confi gurable, fault-tolerant processor (CFTP) experiment, and fl ight demonstrations of commercial, off-the-shelf (COTS) components such as microelectromechanical systems (MEMS) rate sensors and a digital camera. The spacecraft command and data handling (C&DH) subsystem is NPS-designed, featuring low-power with error-detection-and-correction (EDAC) memory, an ARM720T microprocessor, and running Linux as the operating system. Two other experiments are provided by the Naval Research Laboratory to investigate ionospheric physics. This paper presents an overview of the spacecraft, its subsystems, and the challenges of a small satellite program in a university environment.Naval Postgraduate School, Monterey, California

Introducing Dialogue to Graduate Students

Scholars and practitioners in adult education commonly agree on the importance of communication and interaction in the adult classroom. Frequently, however, learners are not provided with guidelines on how to interact and communicate with others in such settings. This paper reports on an experiential orientation to the practice of dialogue for adults in a graduate progra

Moodling Around: A Tool for Interactive Technologies

Virtual Learning Environments (VLEs) are becoming a widespread means of facilitating learning. The Moodle VLE is one of many technology tools that enable and support online learning. It is a user-friendly, free, opensource tool used to deliver instruction online and to supplement face-to-face learning

Phosphatases Generate Signal Specificity Downstream of Ssp1 Kinase in Fission Yeast

AMPK-related protein kinases (ARKs) coordinate cell growth, proliferation, and migration with environmental status. It is unclear how specific ARKs are activated at specific times. In the fission yeast Schizosaccharomyces pombe, the CaMKK-like protein kinase Ssp1 promotes cell cycle progression by activating the ARK Cdr2 according to cell growth signals. Here, we demonstrate that Ssp1 activates a second ARK, Ssp2/AMPKα, for cell proliferation in low environmental glucose. Ssp1 activates these two related targets by the same biochemical mechanism: direct phosphorylation of a conserved residue in the activation loop (Cdr2-T166 and Ssp2-T189). Despite a shared upstream kinase and similar phosphorylation sites, Cdr2 and Ssp2 have distinct regulatory input cues and distinct functional outputs. We investigated this specificity and found that distinct protein phosphatases counteract Ssp1 activity toward its different substrates. We identified the PP6 family phosphatase Ppe1 as the primary phosphatase for Ssp2-T189 dephosphorylation. The phosphatase inhibitor Sds23 acts upstream of PP6 to regulate Ssp2-T189 phosphorylation in a manner that depends on energy but not on the intact AMPK heterotrimer. In contrast, Cdr2-T166 phosphorylation is regulated by protein phosphatase 2A but not by the Sds23-PP6 pathway. Thus, our study provides a phosphatase-driven mechanism to induce specific physiological responses downstream of a master protein kinase