Effect of different salinity concentration on kidney of benni, Barbus sharpeyi

For this study, 144 healthy Barbus sharpeyi with an average weight of 350 ± 2.36 grams and length 25 ± 1.25 cm in five groups were studied. The first group as control located in municipal dechlorination water and the next four groups respectively were kept in salinity 4ppt, 8ppt, 12ppt and 16ppt in the same condition. On days 1 , 3 , 7 , 14 , 21 and 28 sample of kidney with maximum thickness of 0.5 cm prepare and were placed in bouin's solution.Then the standard method of parafin sections were done and 5- 6 micrometer thick of tissue sections prepared and stained with H&E methods. Results showed the gradual transfer of fish to water with high salinity caused obvious changes as increase the number and diameter of the glomeruli especially in high salinity but the severity was reduced at the end of the period (p<0.05). Also highest diameter and thickness of the collecting tubules were reported in fresh water at 28 days (p<0.05). These findings suggest that fish Barbus sharpeyi was friendly with salinity and ability to set vital to different salinity

Effects of different salinity on number and area of chloride cells in gill of juvenile grouper (Epinephelus coioides)

The purpose of this study was to evaluate the ability of osmoregulation by mitochondria-rich cells in grouper fish with high tolerance of salinity variation levels. For this purpose, groupers were transferred from salt water with an average salinity of 40 ppt to the waters with 10 ppt, 20 ppt and 60 ppt rates of salinity. Changes in the number and area of chloride cells in the different treatments were observed during two months adjustment period. Experiment was carried out by sampling three fish from each tank within 8 phases at the moment of the transition, 12 hours, day 1, day 3, day 7, day 14, day 30 and day 60. Histomorphological and immunohistochemical observations were done after fixing in Bouin's solution for 24 hours. Samples were dehydrated with increasing series of ethanol, followed by paraffin, and cleared by xylene. Paraffin blocks were cut at 5 microns and stained by hematoxylin – eosin. Changes in the number and area of chloride cells in 5% level were very different at high and brackish salinity. So in the early hours of the transfer, number and area of chloride cells were low in the brackish water than to that in high salinity. From second week towards the end of period, number of chloride cells in 60 ppt was more than that in control treatment from seventh days towards the end of period, the area of chloride cells was more than that in control group. The high ability and adaptability of this species in response to different environmental salinities might be associated with changes in gill histological evaluation and model osmoregulation mechanisms at different salinities

Effect of different salinity concentration on different areas of the body skin of benni, Barbuss harpeyi

For this purpose, 144 specimens were studied in five healthy groups. The first group was the control group put in dechlorinated tap water and four respectively in the next group were kept with salinity 4ppt, 8ppt, 12ppt, 16ppt in the same condition. In days (1, 3, 7, 14, 21, 28) samples were collected from skin of head, ventral and lateral lines. Sections with 5- 6µm thickness were made and stained with PAS and H&E methods. In histometrical survey, epiderm thickness, numbering of club cells and goblet cells in 200 µm of epiderm length in any area, was investigated comparatively. The results showed that in different salinity concentrations, skin of head, ventral and lateral lines were influenced and changes in epiderm thickness, reduce the number of club cells and increase the number of goblet cells were demonstrated. These changes in different concentrations for each parameter, for significantly increased (p<0.05)

Anatomical and histological study of kidney in Barbus grypus

At this study, kidneys of 10 normal fishes have been studied macroscopic and microscopically. The kidneys were collected by autopsy and tissue samples were sectioned by routine paraffin embedding and stained by H&E and PAS. Macroscopic observation showed that the kidney in Barbus grypus situated in the ventral region of vertebral column. The kidney could be divided into; head, body and tail portions according to their relative position. Microscopic studies showed that the kidneys consisted of excretory portion and hematopoietic-lymphoid tissues. Anterior portion of kidney (head) had more Hematopoietic lymphoid tissues than the posterior portion (body and tail). Hematopoietic-lymphoid tissue consisted of cells which were found in different stages of development to blood cells. Excretory portion of kidney parenchyma consisted of renal corpuscle and urinary tubules. In microscopic section of glomerulus, endothelial and mesangial cells and nucleated red blood cells were seen. The number of renal corpuscles in the posterior portion of kidney was more than the anterior portion significantly, while there was no significant difference between the average diameter of renal corpuscles in the head, body and tail portions of kidney. Urinary tubules consisted of neck segment, proximal tubule (first and second portion), intermediate segment and distal segment. The collecting ducts were situated after the distal segments. No significant difference was observed in the measured parameters between the males and females fishes in this study. Based on the results of this study, the kidney of Barbus grypus is very similar to other species of the family Cyprinidae, histologically and anatomically

The Effects of Vitamin E on Liver and Kidney Damage Induced by Dianabol in Small Laboratory Mice

BACKGROUND AND OBJECTIVE: Anabolic steroids, especially dianabol, are used by athletes as a performance-enhancing drugs that damage the liver and cause structural changes. The aim of this study was to evaluate the effects of vitamin E on liver and kidney toxicity caused by dianabol. METHODS: In this experimental study, 72 adult male mice were randomly divided into 8 groups of 9. Four groups of mice received 100 IU / kg vitamin E orally for 42 days through gavage. Three groups of the above groups received 5, 10 and 20 mg / kg oral dianabol four hours after receiving vitamin E, respectively. The control group and the groups receiving only 5, 10 and 20 mg / kg oral dianabol were also considered. 24 hours after the final treatment, serum samples were collected for biochemical evaluations and tissue samples were collected for histological, histomorphometric and histochemical evaluations. FINDINGS: The results showed that dianabol significantly increased the level of AST (158.52±9.76), ALT (113.70±11.02), and ALP (141.30±5.94), and significantly decreased albumin (1.04±0.47) compared to the control group (72.61±7.54, 41.47±7.03, 112.80±4.30, 3.14±0.25, respectively) (p<0.05). Administration of vitamin E significantly increased the level of AST (110.56±9.86), ALT (80.19±4.02) and ALP (120.52±4.94) and improved albumin (2.1±0.28) (p<0.05). CONCLUSION: The results of the study showed that vitamin E can reduce the oxidative damage caused by dianabol in the liver and kidney of the mouse

Histological and histomorphometrical study of intestinal bulb in shirbot, Barbus grypus

Barbus grypus is native fishes in Khuzestan province which its species with a wide distribution in the rivers that considered fisheries research in province. The fish have no stomach, space of intestine is narrow to store of food, for this reason anterior intestine dilated bowel and called intestinal bulb. Study of histological structure of intestinal bulb in fishes can help to nutrition and food needs so that we can extend of this species. In this study, 10 adults Barbus grypus of both sexes with average weigh 354.25 ± 60.52 g and average length 36.25 ±4.16 cm was chosen, and after easy drawing, samples were removed from intestinal bulb. The 0.5cm were fixed by Bouin´s fixative soluble and 5-6µ thickness sections were made by paraffin embedding method and were stained by H&E and PAS. The histomorphometrical studies were done using digital Dino-Lite lens and Dino-capture1 software. The results showed that the number of goblet cells in the intestine increased toward the end of the intestinal bulb. The intestinal epithelium height in the anterior part of the intestinal bulb was higher than other parts. Muscular thickness was different in different parts of the intestinal bulb; the middle part of intestinal bulb has thickest muscle between other parts of intestinal bulb. The results of this research indicate that there is some analog histological structure of the intestinal bulb in Barbus grypus to compare of other same family: from wall diameter and intestinal epithelium height and diameter of mucosa

The Effect of Wheat Sprout Extract on Skin Injury Following Injection of Lead Acetate in Rat

Abstract &nbsp; Background & aim: Skin is constantly exposed to environmental contaminants such as heavy metals (lead).Medicinal plants have been concern for the treatment of of human pain. and pain. Wheat Sprout is one of medicinal plants which are rich in vitamins, minerals and powerful antioxidant compounds respectively. The aim of this study was to evaluate the effect of wheat Sprout extract on tissue texture following injection of lead acetate in rats. &nbsp; Methods: Thirty healthy adult Wistar rats were divided randomly into six groups: Control group received 1 ml/kg/day of normal saline, group 2 received 20 mg/kg/day of lead acetate intraperitoneally respectively, group 3 and group 4 received 100 mg/kg/day and 200 mg/kg/day of wheat sprout extract by gavage feeding, group 5 and group 6 received 100 mg/kg/day and 200 mg/kg/day of wheat sprout extract by gavage feeding along with 20 mg/kg/day of lead acetate intraperitoneally. After five weeks, skin tissue of dorsal region and blood samples were collected for histomorphometric studies and serum assessment. Serum samples were tested for determining antioxidant activity (AOA) based on power ferric reduction antioxidant (FRAP) assay and peroxidation of lipids by measuring malondialdehyde (MDA). The 5 to 6 &mu;m thickness sections were made using paraffin embedding method after stained by hematoxylin and eosin, safranin and masson trichrome. For microscopic study, Dino-Lite digital lens and Dino Capture 2 Software were used. &nbsp; Results: The lead significantly decreased the total thickness of the skin, the dermal layer, hypoderm, the number and maximum depth of hair follicles and the epidermis pod thickness of the hair root compared with the control group (p <0.05). A significant increase was seen in numbers of sebaceous glands and hair follicles in group receiving 200 mg/kg/day of wheat sprout, compared to that in control group (P <0.001). Wheat sprout simultaneously with lead increased the epidermis sheath thickness of the root (p <0.01) but no significant increase was seen in the depth of hair follicles in comparison with the lead group.MDA level showed a significant increase in lead group, compared to control group (P <0.01). AOA level showed a significant increase in wheat sprout (200 mg/kg/day) group, compared to other groups (P <0.001). &nbsp; Conclusion: The results showed that lead can induce negative effects in skin tissues. Wheat sprout extract (200 mg/kg/day) can inhibit toxic effects of lead in skin tissues and that leads to refreshing in skin. &nbsp; &nbsp

Pharmacological, histopathological, and biochemical assessments of cutaneous wound healing potential of Tragopogon graminifolius aqueous extract ointment in rats

The recent experiments have revealed the property of ethno-medicinal plants on the treatment of the cutaneous wound. The purpose of the experiment was to investigate the wound healing potential of Tragopogon graminifolius aqueous extract ointment. DPPH free radical scavenging test was carried out to examine the antioxidant effect of T. graminifolius aqueous extract, which indicated similar antioxidant activity with butylated hydroxy toluene (BHT) as a positive control. In vivo design, 120 Sprague Dawley male rats were used. After creating the cutaneous wound, the animals were randomly divided into four groups; untreated control, treatment with Eucerin ointment, treatment with 3 tetracycline ointment, and treatment with 3 T. graminifolius aqueous extract ointment (3 g of T. graminifolius aqueous extract + 97 g base ointment). On days 10, 20, and 30 after creating the wound, for histopathological and biochemical analysis of the cutaneous wound healing trend, a section was prepared from all dermal thicknesses. The data were analyzed using one-way ANOVA followed by Duncan post hoc test. On days 10, 20, and 30, T. graminifolius aqueous extract ointment could significantly (p ≤ 0.05) decrease the level of the wound area, total cell, macrophage, lymphocyte, and neutrophil, and enhance the level of wound contracture, fibrocyte, hexuronic acid, hexosamine, and hydroxyproline as compared to the basal ointment and control groups. In conclusion, the acquired findings showed the cutaneous wound healing potential of T. graminifolius aqueous extract ointment. © 2019, Springer-Verlag London Ltd., part of Springer Nature