The Effects of Vitamin E on Liver and Kidney Damage Induced by Dianabol in Small Laboratory Mice

Abstract

BACKGROUND AND OBJECTIVE: Anabolic steroids, especially dianabol, are used by athletes as a performance-enhancing drugs that damage the liver and cause structural changes. The aim of this study was to evaluate the effects of vitamin E on liver and kidney toxicity caused by dianabol. METHODS: In this experimental study, 72 adult male mice were randomly divided into 8 groups of 9. Four groups of mice received 100 IU / kg vitamin E orally for 42 days through gavage. Three groups of the above groups received 5, 10 and 20 mg / kg oral dianabol four hours after receiving vitamin E, respectively. The control group and the groups receiving only 5, 10 and 20 mg / kg oral dianabol were also considered. 24 hours after the final treatment, serum samples were collected for biochemical evaluations and tissue samples were collected for histological, histomorphometric and histochemical evaluations. FINDINGS: The results showed that dianabol significantly increased the level of AST (158.52±9.76), ALT (113.70±11.02), and ALP (141.30±5.94), and significantly decreased albumin (1.04±0.47) compared to the control group (72.61±7.54, 41.47±7.03, 112.80±4.30, 3.14±0.25, respectively) (p<0.05). Administration of vitamin E significantly increased the level of AST (110.56±9.86), ALT (80.19±4.02) and ALP (120.52±4.94) and improved albumin (2.1±0.28) (p<0.05). CONCLUSION: The results of the study showed that vitamin E can reduce the oxidative damage caused by dianabol in the liver and kidney of the mouse