70 research outputs found
Frequency of Blood Types A, B and AB in a Population of Non-Pedigree Domestic Cats from Central Italy
Blood transfusion reactions and neonatal isoerythrolysis are common events in the feline population due to the presence of natural alloantibodies in the AB blood group system. It is known that the frequency of feline blood types varies according to the geographic region and breed. Therefore, the aims of this study were to investigate the frequency of AB blood groups in non-pedigree domestic cats in Central Italy and estimate the risk of a life-threatening transfusion reaction and neonatal isoerythrolysis, caused by mismatched transfusion or incompatible random mating, respectively. The AB blood group was determined on non-pedigree domestic feline patients and potential blood donors submitted at the Veterinary Teaching Hospitals of the Universities of Teramo (Abruzzo Region, Teramo, Italy) and Perugia (Umbria Region, Teramo, Italy), and visited at veterinary practitioners in Rome (Lazio Region, Teramo, Italy) using commercial immunochromatographic cartridges and commercial agglutination cards. There were four hundred and eighty-three cats included in the study. The frequencies of the blood types were: 89.9% type A, 7.0% type B, and 3.1% type AB. The probability of an acute hemolytic transfusion reaction or a neonatal isoerythrolysis was 6.5%. Although the frequency of type B in non-pedigree domestic cats living in Central Italy was relatively low, to reduce the risk of fatal transfusion reactions, blood group typing is recommended before each transfusion
Triple-Phase Multidetector Computed Tomography in Distinguishing Canine Hepatic Lesions
The liver has a unique vascular supply, and triple-phase contrast-enhanced computed
tomography examinations are being performed in order to characterize liver lesions. This study
aimed to look for any associations between the attenuation values of liver lesions and their histological
classification. The inclusion criteria for this retrospective study were focal or multifocal liver lesions
and histological diagnosis. All of the dogs underwent pre-contrast and triple-phase postcontrast
computed tomography (CT) examinations with identical timings of the postcontrast series. Thirtyone dogs were included in the study, and various benign and malignant pathologies were identified.
The results did not identify any significant differences between the benign and malignant liver
lesions, nor between the individual histological diagnoses. Inflammatory lesions were significantly
different compared to the normal liver parenchyma, and significant hypoattenuation was found in
the portal and delayed venous phases. Hemangiosarcomas were significantly hypoattenuating to the
normal liver parenchyma in the pre-contrast and arterial phases, and also to all of the benign lesions
in the arterial phase. The other pathologies showed variable attenuation patterns in the different
postcontrast phases, and differentiation was not possible. On the basis of this study, triple-phase
contrast-enhanced computed tomography cannot differentiate between benign and malignant liver
lesions, and biopsy and further histological analysis are necessar
controlled field study evaluating the clinical efficacy of a topical formulation containing emodepside and praziquantel in the treatment of natural cat aelurostrongylosis
BackgroundAelurostrongylus abstrusus is the most important nematode affecting the respiratory tract of cats in terms of prevalence and clinical relevance. The aim of this randomised controlled field study was to confirm the efficacy of the spot-on containing emodepside/praziquantel (Profender, Bayer Animal Health) in the treatment of aelurostrongylosis.MethodsSeventeen cats with aelurostrongylosis and presenting with clinical and/or radiographic signs were included in the study. Eight cats received two biweekly doses of emodepside/praziquantel, while nine cats were allocated to a control group and received a rescue treatment at the end of the study. Clinical response was the primary outcome, while the secondary end point was the reduction of larval shedding in faeces.ResultsTwo weeks after the first application, the cats showed a significant, though partial, recovery of clinical signs with complete clinical and parasitological resolution. The resolution of inflammatory leucogram and a significant reduction of radiographic lesions were observed two weeks after the second treatment. Red blood cells and albumin values significantly increased after eight weeks from the second application, together with the complete regression of radiographic patterns.ConclusionTwo applications of this spot-on solution two weeks apart assured complete cessation of larval shedding and led to a complete clinical, clinicopathological and radiographic recovery
Invasive pleural malignant mesothelioma with rib destruction and concurrent osteosarcoma in a dog
A 7-year-old Dachshund was clinically examined because of a 10-day history of lameness in the left hind limb. On the
basis of radiological and cytological findings, an osteosarcoma of the left acetabular region was suspected. The dog
underwent a hemipelvectomy and osteosarcoma was diagnosed by subsequent histopathological examination. An
immovable subcutaneous mass was noted on the left chest wall during the physical examination and non-septic neutrophilic
inflammation was diagnosed by cytology. Forty days later, the dog showed signs of respiratory distress with
an in-diameter increase of the subcutaneous mass up to 4 cm. Thoracic radiography and ultrasonography revealed
pleural effusion and a lytic process in the fourth left rib. Furthermore, ultrasound examination revealed a mixed
echogenic mobile structure with a diameter of around 2 cm floating within the pleural fluid of the left hemithorax
close to the pericardium. The dog underwent surgery for an en bloc resection of the subcutaneous mass together
with the fourth rib and the parietal pleura. Moreover, the left altered lung lobe, corresponding to the mobile structure
detected by ultrasound, was removed. Based on cytological, histopathological, and immunohistochemical examinations,
an invasive epithelioid pleural malignant mesothelioma was diagnosed
ZOOMICS: comparative metabolomics of red blood cells from dogs, cows, horses and donkeys during refrigerated storage for up to 42 days
The use of omics technologies in human transfusion medicine has improved our understanding of the red blood cell (RBC) storage lesion(s). Despite significant progress towards understanding the storage lesion(s) of human RBCs, a comparison of basal and post-storage RBC metabolism across multiple species using omics technologies has not yet been reported, and is the focus of this study
Evaluation of Bronchoscopy and Bronchoalveolar Lavage Findings in Cats With Aelurostrongylus abstrusus in Comparison to Cats With Feline Bronchial Disease
The cat lungworm Aelurostrongylus abstrusus is a cause of lower respiratory tract disease worldwide. Bronchoscopy and bronchoalveolar lavage (BAL) are important tools for diagnosing respiratory diseases in cats. Therefore, the aim of the study was to investigate the usefulness of bronchoscopy and BAL in the diagnosis of A. abstrusus. Findings from bronchoscopic examination and BAL of 24 naturally infected cats were evaluated and compared with those of 12 cats with idiopathic Feline Bronchial Diseases (FBDs). Data were analyzed using Mann-Whitney or Fisher's exact tests. No significant bronchoscopic differences were detected between cats with aelurostrongylosis and FBDs in bronchial mucus, nodular lesions, and airway collapse. On the other hand, airway hyperemia, epithelial irregularities, and bronchial stenosis were observed more frequently in cats affected by FBDs than aelurostrongylosis, while bronchiectasis was found only in cats infected by A. abstrusus. Neutrophilic, eosinophilic, lymphocytic, and mixed inflammation were recorded in both groups. Bacteria or bacterial DNA was identified regardless of the presence or absence of A. abstrusus with no significant differences between groups. Larvae of A. abstrusus were cytologically detected in 5 of the 24 cats (20.8%) with aelurostrongylosis. These results indicate that, although some findings on bronchoscopic examination (i.e., bronchiectasis) can be described more frequently in cats infected by A. abstrusus, bronchial alterations and cytological findings in aelurostrongylosis are not specific unless larvae are observed and overlap with those of other feline airway diseases
Clinical Value of CT-Guided Fine Needle Aspiration and Tissue-Core Biopsy of Thoracic Masses in the Dog and Cat
Diagnosis of thoracic lesions on the basis of history and physical examination is often challenging. Diagnostic imaging is therefore of paramount importance in this field. Radiology has traditionally been considered the diagnostic procedure of choice for these diseases. Nevertheless, it is often not possible to differentiate inflammatory/infectious lesions from neoplastic diseases. A correct cytological and histopathologic diagnosis is therefore needed for an accurate diagnosis and subsequent prognostic and therapeutic approach. In human medicine, Computed Tomography (CT) and CT-guided biopsy are used in the presence of lesions which are not adequately diagnosed with other procedures. In the present study, thoracic lesions from 52 dogs and 10 cats of different sex, breed and size underwent both CT-guided fine-needle aspiration (FNAB) and tissue-core biopsy (TCB). Clinical examination, hematobiochemical analysis and chest radiography were performed on all animals. In this study, 59 of 62 histopathological samples were diagnostic (95.2%). Cytology was diagnostic in 43 of 62 samples (69.4%). General sensitivity, accuracy and PPV for FNAB and TCB were 67.7%, 67.7% and 100% and 96.7%, 95.2% and 98.3%, respectively. Combining the two techniques, the overall mean accuracy for diagnosis was 98.4%. Nineteen of 62 cases showed complications (30.6%). Mild pneumothorax was seen in 16 cases, whereas mild hemorrhage occurred in three cases. No major complications were encountered. CT-guided FNAB cytology can be considered a useful and reliable technique, especially for small lesions or lesions located close to vital organs and therefore dangerous to biopsy in other way
Early Renal Involvement in Cats with Natural Feline Morbillivirus Infection
Feline morbillivirus (FeMV) is a newly discovered paramyxovirus infecting domestic cats and its role in the pathogenesis of feline chronic kidney disease (CKD) has been suggested, however not confirmed. The primary aim of the study was to evaluate the renal damage associated with FeMV infection in cats. In this retrospective study, clinical and clinicopathological data were compared among 14 FeMV naturally infected, 21 CKD and 22 healthy cats. FeMV positive cats had serum chemistry analytes and main urine chemistry results similar to the healthy subjects. FeMV positive cats had significantly decreased urine specific gravity (median 1054, range 1022-1065) and urine creatinine (median 227.23 mg/dL, range 83.02-489.75) when compared with healthy cats (median 1067, range 1040-1080, P < 0.001; median 406.50 mg/dL, range 195.32-575.58; P < 0.001, respectively). Urine protein:creatinine ratio (UPC) results of FeMV and CKD were not different (median 0.20, range 0.08-1.03; median 0.23, range 0.10-0.80, respectively), however UPC results were significantly increased in both groups, if compared with healthy cats (median 0.1, range 0.04-0.250, P < 0.01). Based on clinical data, serum creatinine concentration, urine specific gravity and UPC results, CKD was suspected by clinicians in 3/14 FeMV cats. Urine protein sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in 10/13 (77%) FeMV cats indicated a tubular pattern, with a decrease of uromodulin and an increase in the number and intensity of low molecular weight proteins. FeMV infection can be associated with different grades of renal dysfunction ranging from mild tubular proteinuria with less concentrated urine to azotemia in cats younger than those typically affected by CKD
Invasive pleural malignant mesothelioma with rib destruction and concurrent osteosarcoma in a dog
A 7-year-old Dachshund was clinically examined because of a 10-day history of lameness in the left hind limb. On the
basis of radiological and cytological findings, an osteosarcoma of the left acetabular region was suspected. The dog
underwent a hemipelvectomy and osteosarcoma was diagnosed by subsequent histopathological examination. An
immovable subcutaneous mass was noted on the left chest wall during the physical examination and non-septic neutrophilic
inflammation was diagnosed by cytology. Forty days later, the dog showed signs of respiratory distress with
an in-diameter increase of the subcutaneous mass up to 4 cm. Thoracic radiography and ultrasonography revealed
pleural effusion and a lytic process in the fourth left rib. Furthermore, ultrasound examination revealed a mixed
echogenic mobile structure with a diameter of around 2 cm floating within the pleural fluid of the left hemithorax
close to the pericardium. The dog underwent surgery for an en bloc resection of the subcutaneous mass together
with the fourth rib and the parietal pleura. Moreover, the left altered lung lobe, corresponding to the mobile structure
detected by ultrasound, was removed. Based on cytological, histopathological, and immunohistochemical examinations,
an invasive epithelioid pleural malignant mesothelioma was diagnosed
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