Evaporation of bi-component droplets in a heated, highly turbulent flow

This work aims to understand the phenomena that occur in a combustion chamber where multi-component fuel droplets are injected. Many evaporation models exist but the influence of turbulence on spray vaporization is not yet well understood. This study gives a useful database to improve these models. The objective of the work is to measure the dispersion and the evaporation of bi-component (octane/3-pentanone) droplets and the resulting vapor mixing in a well-known, heated, highly turbulent channel flow. The carrier flow shows high turbulence levels, flat profiles for the mean velocity and the velocity fluctuations. The injected droplets have a large variety of behaviors due to the large polydispersion and to the turbulence. The evolution of 3-pentanone liquid concentration, mass flux, and droplet clusters are described. Mean concentration, fluctuations of concentration, and mixing of the vapor phase are characterized

Évaporation et dispersion d'un spray bi-composant dans un écoulement de canal chauffé fortement turbulent : une approche expérimentale

Cette étude s'inscrit dans le cadre de la compréhension des phénomènes ayant lieu dans les chambres de combustion aéronautiques. Ces phénomènes étant multiples et complexes, des simplifications sont nécessaires. L'étude se focalise uniquement sur l'évaporation de gouttelettes bi-composant en écoulements turbulents. De nombreux modèles d'évaporation existent mais l'influence de la turbulence dans le cas d'un spray est encore mal comprise. Alors que la turbulence augmente l'évaporation d'une goutte isolée, elle peut amener à la création d'amas de gouttes qui vont au contraire ralentir l'évaporation. Cette étude a donc pour but de fournir un certain nombre de données quantitatives permettant une meilleure compréhension de ces phénomènes et une amélioration des modèles. L'approche est expérimentale. L'objectif est de quantifier, d'une part l'évaporation et la dispersion de gouttelettes, d'autre part le mélange vapeur dans un écoulement de canal dont les caractéristiques seront connues. Afin de simplifier les conditions expérimentales, la température est moins élevée que dans le cas réel et la pression est la pression atmosphérique. De plus, les gouttelettes sont bi-composant (octane/3-pentanone). Le banc utilisé est divisé en deux parties. Sa partie supérieure est composée d'un système de génération de l'écoulement turbulent et d'un injecteur de gouttelettes. Sa partie inférieure est composée d'une veine dans laquelle l'écoulement diphasique est analysé. L'écoulement porteur est étudié sans le spray par Anémométrie Laser Doppler. L'écoulement présente une forte turbulence, des profils plats de vitesses moyennes et de f uctuations de vitesses, en zone établie. Les propriétés d'isotropie et la décroissance de la turbulence sont proches de celles obtenues en turbulence de grille. La phase dispersée est suivie à chaud à l'aide de deux méthodes : par Anémométrie Phase Doppler et par Fluorescence Laser Induite. L'Anémométrie Phase Doppler permet d'avoir accès simultanément au diamètre et à la vitesse de chaque goutte passant à travers le volume de mesure. En raison de la forte polydispersion, les comportements des gouttelettes vis à vis de la turbulence sont très différents. En revanche, l'homogénéisation est rapide quelque soit la classe de taille. La présence d'amas dont la quantité diminue en aval dans la veine est mis en avant. La Fluorescence Laser Induite mesure la quantité de molécules de 3-pentanone en phase liquide. L'évolution de la concentration liquide, des flux de masse et des amas est décrite. La Fluorescence Induite par Laser permet aussi de suivre la quantité de 3-pentanone en phase vapeur. L'évolution des prof ls radiaux et axiaux de concentration moyenne et des f uctuations de concentration est présentée. L'homogénéisation du mélange est quantifiée. ABSTRACT : This work aims to understand the phenomena that occur in a combustion chamber. Due to the complexity of the phenomena encountered, simplifications are made. This study only focuses on multicomponent droplet evaporation in turbulent flows. Many evaporation models exist, but the influence of turbulence on a spray is yet not well understood. On one hand, turbulence increases the droplet evaporation rate. On the other hand, it may generate clusters, in which saturation stops the process. This study aims to give a database that can be used to improve the physical understanding of the process and to improve model performances. This is an experimental approach. The objective is to measure evaporation and dispersion of droplets and vapour mixing in a well-known turbulent flow. In the simplified test case studied here, the temperature is lower than in a real case and the pressure is atmospheric. The droplets are bi-component(octane/3-pentanone). The experimental set-up is divided into two parts. The first part, at the top, consists in a turbulence flow generator and a droplet injection device. The second part is a channel in which the two- hase flow is analysed. The carrier flow is measured using Laser Doppler Anemometry. The main flow properties are : high turbulence levels, flat profiles for the mean velocity and velocity fluctuations. The turbulence decreases and isotropic properties are close to those of grid turbulence. The dispersed phase is measured using Phase Doppler Anemometry (PDA) and Laser Induced Fluorescence (LIF). The velocity and diameter of each droplet passing through the measurement volume is measured by the PDA technique. There is a large variety of droplet behaviours due to the large polydispersion and turbulence. Droplet clusters are measured. Their amounts decrease with the distance from the injector. The concentration of 3-pentanone can be measured with the LIF technique. The evolution of the liquid concentration, mass flux and droplet clusters is described. The mean vapour concentration and its fluctuations are measured along the axial and radial axis. The mixing of the vapour is characterise

Inside-out: unpredicted Annexin A2 localisation on the surface of extracellular vesicles

This is the author accepted manuscript. The final version is available from Science Matters via https://www.sciencematters.io/articles/201602000015Inside-out: unpredicted Annexin A2 localisation on the surface of extracellular vesicles Extracellular vesicles (EVs) contain many proteins, both cytosolic and surface bound. The current model for EV biogenesis dictates that cytosolic proteins remain in the lumen and cell surface proteins reside on the outside of vesicles. This is consistent with the traditional protein trafficking pathway, where proteins destined for the plasma membrane contain a signal sequence targeting them to the secretory pathway. According to this ‘classical’ pathway for membrane and secretory protein trafficking, proteins lacking a signal sequence should not reside at the cell surface. It has been shown that transmembrane proteins are retained in the membrane of EVs and RNAs reside in the lumen of EVs. However, there is little known about the packaging and location of other proteins enriched in EVs. Annexin A2 is a cytosolic protein abundant in EVs. We show for the first time that Annexin A2 is expressed not only in the lumen of EVs as predicted but also on the surface of EVs. This raises fundamental questions regarding Annexin A2 transport to the outer leaflet of the EV membrane as it lacks a signal peptide for secretion.This work was supported by Wellcome Trust Strategic Award [100574/Z/12/Z] and MRC Metabolic Diseases Unit [MRC_MC_UU_12012/5], from the Italian Multiple Sclerosis Association (AISM, grant 2010/R/31), the Italian Ministry of Health (GR08-7), the European Research Council (ERC) under the ERC-2010-StG Grant agreement n° 260511-SEM_SEM, the UK Regenerative Medicine Platform Acellular hub (Partnership award RG69889), and core support grant from the Wellcome Trust and MRC to the Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute. FG is supported by a scholarship of the Gates Cambridge Trust

Vaporization of bi-component droplets in a turbulent over-heated flow : Experiments and Numerical Simulation

This paper deals with an experimental study of bi-component droplets evaporation in a turbulent over-heated channel flow. Droplets are made of a mixture of n-octane and 3-pentanone (15% in volume). Planar Laser Induced Fluorescence (PLIF) of 3-pentanone is used to derive both concentration of this molecule in liquid and gas phases. Concomitant dispersion of the liquid droplets and evaporation can be clearly observed with a trend to homogenisation of the 3-pentanone vapour in the flowfield due to high turbulence rate, on the order of 40%. The experimental results, in term of concentration of 3-pentanone in both liquid and gas phases have been compared to a simplified numerical simulation based on the discrete component model, taking into account the droplet composition, associated to the isolated droplet model. It appears that the measurements performed on the liquid phase overestimate the calculated evaporation, as the gas phase measurements performed far from the injection point are in correct agreement with the calculations. One of the explanations is that the PLIF CCD camera dynamic is not sufficient to detect simultaneously the biggest and smallest droplets. In the present cases, most of the numerous smallest droplets seem to be ignored by the measurement, which tends to overestimate the droplet evaporation. The second important cause of discrepancy between experimental and numerical results is linked to the strong extinction of the fluorescence signal issuing from the liquid phase, due to the absorptio

A 9,9'-spirobifluorene bases Metal-Organic Framework: systhesis, structure analysis and gas sorption properties

International audienceThe new square planar tetracarboxylate ligand L (4,4′,4′′,4′′′-(9,9′-spirobi[fluorene]-2,2′,7,7′-tetrayl)tetrabenzoic acid) was synthesized and used for synthesis of the Metal-Organic Framework Cu2L(H2O)2*(EtOH)4 denoted SBF-Cu. This material possesses the classical 4-4 regular tiling topology with paddle-wheel inorganic building units. Due to the presence of SBF cores, the interactions between the layers of this MOF confer it specific properties: high specific surface area, open metal sites under activation, and promising hydrogen uptake capacity

Computational evaluation of the impact of incorporated nitrogen and oxygen heteroatoms on the affinity of polyaromatic ligands for carbon dioxide and methane in metal–organic frameworks

Density functional theory is employed to explore the binding of carbon dioxide and methane in a series of isoreticular metal–organic frameworks, with particular emphasis on understanding the impact of directly incorporated nitrogen and oxygen heteroatoms on the affinity of the ligand for CO2 and CH4. While the strongest binding sites for both CO2 and CH4 were found to be directly above the aromatic rings of the core of the ligand, the introduction of heteroatoms to the core systems was shown to significantly alter both the binding strength and preferred binding locations of CH4 and CO2. The presence of pyrazine rings within the ligand was observed to create new binding sites for both CO2 and CH4 and, in the case of CO2, severely reduce the binding strength or entirely eliminate binding sites that were prominent in the analogous carbocyclic ligands. These results suggest that while the presence of framework nitrogen and oxygen heteroatoms provides a route to ligands with enhanced affinity for methane, a similar increase in affinity for CO2 is not guaranteed

Computational evaluation of the impact of incorporated nitrogen and oxygen heteroatoms on the affinity of polyaromatic ligands for carbon dioxide and methane in metal–organic frameworks

Density functional theory is employed to explore the binding of carbon dioxide and methane in a series of isoreticular metal–organic frameworks, with particular emphasis on understanding the impact of directly incorporated nitrogen and oxygen heteroatoms on the affinity of the ligand for CO2 and CH4. While the strongest binding sites for both CO2 and CH4 were found to be directly above the aromatic rings of the core of the ligand, the introduction of heteroatoms to the core systems was shown to significantly alter both the binding strength and preferred binding locations of CH4 and CO2. The presence of pyrazine rings within the ligand was observed to create new binding sites for both CO2 and CH4 and, in the case of CO2, severely reduce the binding strength or entirely eliminate binding sites that were prominent in the analogous carbocyclic ligands. These results suggest that while the presence of framework nitrogen and oxygen heteroatoms provides a route to ligands with enhanced affinity for methane, a similar increase in affinity for CO2 is not guaranteed

Etude experimentale de l'evaporation d'un brouillard de gouttelettes dans un ecoulement de canal chauffé et turbulent

L’étude présentée traite de l’évaporation de gouttelettes polydisperses bi-composants dans un écoulement de canal chauffé et très fortement turbulent. Les diamètres et les composantes axiale et radiale de la vitesse des gouttelettes sont mesurés par anémométrie phase doppler