Unravelling Antarctica’s past through the stratigraphy of a deep ice core: an image-analysis study of the EPICA-DML line-scan images

Polar ice research has undergone great progress in the last six decades. One of its recent technological achievements has been the development of new techniques for digital image recording and analysis of ice-core stratigraphy and microstructure. In this work we investigate one such image records, namely the line-scan image records of the EPICA-DML (European Project for Ice Coring in Antarctica, Dronning Maud Land) deep ice core. These images provide a multiscale depiction of the stratigraphy and structure of the Antarctic Ice Sheet. While previous studies have focused on the ice-core optical stratigraphy on the micro- and mesoscale (<1 mm and 10−3–1 m, respectively), in this work we present several methods to obtain fast and reliable information on the ice-core stratigraphy on the macroscale (1–103 m), including the full ice-sheet thickness. The paleoclimatic relevance of the ice-core optical stratigraphy on the macroscale is demonstrated through the comparison of the line-scan grey-value record of the EPICA-DML deep ice core with its mineral dust record, which is used as a proxy for microinclusions and for several other types of climate proxies. Additionally, we introduce a novel method to estimate the macroscopic air-bubble concentration (including number and size of bubbles) in ice cores, which is simpler, faster, and almost as reliable as painstaking microscopic studies. After a brief excursion on the relation between macroscopic and mesoscopic measures of optical stratigraphy, we close this work by making the case for a multi-measure analysis of ice-core line-scan images, which enables us to obtain a broad perspective of the optical stratigraphy of the whole ice core, with relevance for paleoclimate and ice-sheet-flow studies.This research is supported by the Spanish Government through the María de Maeztu excellence accreditation 2018–2022 (Ref. MDM-2017-0714), and by the Basque Government through the BERC 2018–2021 programme. SHF acknowledges support from the Spanish Ministry of Science, Innovation, and Universities (MCIU) through the project iMechPro (RTI2018–100696–B–I00), and from the Ramón y Cajal grant RYC–2012–12167 of the Spanish Ministry of Economy, Industry and Competitiveness (MINECO

Rapid detection of multidrug-resistant Mycobacterium tuberculosis by multiplex allele-specific polymerase chain reaction

OBJECTIVE: To evaluate a multiplex allele-specifi c polymerase chain reaction (MAS-PCR) to detect multidrugresistant tuberculosis (MDR-TB) clinical isolates and to describe the main mutations conferring resistance to isoniazid (INH) and rifampicin (RMP). DESIGN: Drug-resistant Mycobacterium tuberculosis clinical isolates were tested to detect mutations using MAS-PCR. The genes involved were katG, inhA promoter and rpoB. RESULTS: Among 193 clinical isolates included in the study, 52.6% of the INH-resistant isolates presented a mutation in the katG (315) gene, 28.1% in the inhAP (−15) and 3.0% in both. For the rpoB gene, 60% of the RMP-resistant isolates showed a mutation in codon 531, 17.5% in 526 and 2.5% in 516. Results were compared with those obtained by sequencing, and 100% concordance was obtained for the detection of the mutation in katG (315), 94.1% for inhAP (−15), and 97.8% for rpoB. The global concordance between both methods was 98%. CONCLUSIONS: The MAS-PCR system allowed the simultaneous and rapid detection of approximately 80.0% of the drug-resistant clinical isolates. This method could be used as a rapid and simple screening tool to detect drug-resistant TB in clinical practice.Fil: Imperiale, Belén Rocío. Provincia de Buenos Aires. Ministerio de Salud. Hospital ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Morcillo, N. S.. Provincia de Buenos Aires. Ministerio de Salud. Hospital ; Argentin

Interaction of two modulational instabilities in a semiconductor resonator

The interaction of two neighboring modulational instabilities in a coherently driven semiconductor cavity is investigated. First, an asymptotic reduction of the general equations is performed in the limit of a nearly vertical input-output characteristic. Next, a normal form is derived in the limit where the two instabilities are close to one other. An infinity of branches of periodic solutions are found to emerge from the unstable portion of the homogeneous branch. These branches have a nontrivial envelope in the bifurcation diagram that can either smoothly join the two instability points or form an isolated branch of solutions

Atmospheric corrosion of reference metals in Antarctic sites

This paper presents the results obtained at three Antarctic test sites participating in the >Ibero-American Map of Atmospheric Corrosiveness> (MICAT), a project on atmospheric corrosion carried out during the period 1988-1994 at some 70 sites distributed across 12 countries of the Latin-American region, Spain and Portugal. The three Antarctic sites are located near the coastline. The singular climatic characteristics of Antarctic regions are related with the purity of the air, the absence of rainfall and the formation of ice on the metallic surface during an important part of the exposure time. However, electrochemical activity is possible below ice layers. This situation affects the structure and morphology of corrosion product films and the resulting corrosion rates of metallic surfaces. © 2004 Elsevier B.V. All rights reserved.Peer Reviewe

Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif

Heterotrimeric G proteins are quintessential signalling switches activated by nucleotide exchange on Gα. Although activation is predominantly carried out by G-protein-coupled receptors (GPCRs), non-receptor guanine-nucleotide exchange factors (GEFs) have emerged as critical signalling molecules and therapeutic targets. Here we characterize the molecular mechanism of G-protein activation by a family of non-receptor GEFs containing a Gα-binding and -activating (GBA) motif. We combine NMR spectroscopy, computational modelling and biochemistry to map changes in Gα caused by binding of GBA proteins with residue-level resolution. We find that the GBA motif binds to the SwitchII/α3 cleft of Gα and induces changes in the G-1/P-loop and G-2 boxes (involved in phosphate binding), but not in the G-4/G-5 boxes (guanine binding). Our findings reveal that G-protein-binding and activation mechanisms are fundamentally different between GBA proteins and GPCRs, and that GEF-mediated perturbation of nucleotide phosphate binding is sufficient for Gα activation

Two and three-dimensional oscillons in nonlinear Faraday resonance

We study 2D and 3D localised oscillating patterns in a simple model system exhibiting nonlinear Faraday resonance. The corresponding amplitude equation is shown to have exact soliton solutions which are found to be always unstable in 3D. On the contrary, the 2D solitons are shown to be stable in a certain parameter range; hence the damping and parametric driving are capable of suppressing the nonlinear blowup and dispersive decay of solitons in two dimensions. The negative feedback loop occurs via the enslaving of the soliton's phase, coupled to the driver, to its amplitude and width.Comment: 4 pages; 1 figur

Frequency selection by soliton excitation in nondegenerate intracavity downconversion

We show that soliton excitation in intracavity downconversion naturally selects a strictly defined frequency difference between the signal and idler fields. In particular, this phenomenon implies that if the signal has smaller losses than the idler then its frequency is pulled away from the cavity resonance and the idler frequency is pulled towards the resonance and {\em vice versa}. The frequency selection is shown to be closely linked with the relative energy balance between the idler and signal fields.Comment: 5 pages, 3 figures. To appear in Phys Rev Let

Five-year atmospheric corrosion of cu, cr and ni weathering steels in a wide range of environments

This work considers fourteen weathering steels manufactured by adding Cu, Cr and Ni alloying elements to extra mild steel. The steels were exposed for up to 5 years in different atmospheres. The atmospheric corrosion resistance of the steels was evaluated and the rust layers formed were characterised by X-ray diffraction, optical microscopy and scanning electron microscopy. The addition of Ni, particularly at 3.0 wt%, leads to a notable increase in corrosion resistance. The rusts formed contain lepidocrocite, goethite and spinel phases. X-ray diffraction also detected small amounts of kornelite (industrial atmosphere) and akaganeite (marine atmosphere)